LOCUS       NZ_CP061645             3859 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS40 plasmid pAS40-5, complete
            sequence.
ACCESSION   NZ_CP061645
VERSION     NZ_CP061645.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092224
            Assembly: GCF_014721815.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,365
            CDSs (total)                      :: 4,268
            Genes (coding)                    :: 4,118
            CDSs (with protein)               :: 4,118
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 76 of 150
            Pseudo Genes (incomplete)         :: 73 of 150
            Pseudo Genes (internal stop)      :: 21 of 150
            Pseudo Genes (multiple problems)  :: 16 of 150
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061645.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS40"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS40-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(3460..3859,1..563))
                     /locus_tag="IC777_RS21675"
                     /old_locus_tag="IC777_21660"
     CDS             complement(join(3460..3859,1..563))
                     /locus_tag="IC777_RS21675"
                     /old_locus_tag="IC777_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     gene            complement(850..2751)
                     /locus_tag="IC777_RS21680"
                     /old_locus_tag="IC777_21665"
     CDS             complement(850..2751)
                     /locus_tag="IC777_RS21680"
                     /old_locus_tag="IC777_21665"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2748..3092)
                     /gene="mobC"
                     /locus_tag="IC777_RS21685"
                     /old_locus_tag="IC777_21670"
     CDS             complement(2748..3092)
                     /gene="mobC"
                     /locus_tag="IC777_RS21685"
                     /old_locus_tag="IC777_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_044103698.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLAQIDAT
                     QGNAKKKNQENHYTKLDRDFLLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIG
                     IDQTLKELKK"
                     /name="mobC"
     Typing          complement(857..2751)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.575"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caaaaacgaa cggtatcgaa catcatacag ttacgcccaa gccctgaaac ttcttttaag
       61 tcggttttta gttcctgaag ctctacaaaa tcggctaaat aatttaggtc gtatggttga
      121 acgccaaagg tatgattttc ccatgttccg tgacatggat ttttagtgat taaccccgta
      181 tagccacgat ctgcacctaa cgccaagcta tacgcctgct caatcttggc aaggtactgt
      241 atgacattct gctttgctcg atgcgtggta cacactggag ctaaaagcat atagccaata
      301 tgtgcatgtc cattttggcg atttttggct atccatgttg gggtcggtaa atttgcgtca
      361 gaccacgcaa aataagcgtc ttggcggtct atatcgaaca ccaacgagct aacaagacaa
      421 gggggattat gctgaatgta tggcttctgg attgcggtac gttttgggcg aataatcacc
      481 ccacgcccta aatcatccga gcaatacggt ttttttggga gtttttcgta aaaatcgtct
      541 gaaaattgcg ttttattgag catgaaacac gtccaagtgt tgcattggct caaaacatca
      601 gtaaaataca agtatcgaat ttgagtttta cggatgcttt aagcgtttgt ttaacaaaga
      661 gaaaaaagcg ggtcgccaaa cctgcttttt tttcgcctga tgaaaatatt acaccttttt
      721 gaattgctgt caaatgactg attttcaaaa caaaatattg cgacaaaatg tcgcaaatat
      781 gtgacctttg aagatgcttt tttttcgagg acagcattgc aagggcaatg ctccgcagaa
      841 aaaaaagtat caaaatctag gtgaataact cctgtcattt ttacgttgtt gctccagttg
      901 caactctcta cgtctgtcca gttcatctaa agccttgaag atatttgttt gatcaaaccc
      961 tcgaattttt cgtgatactg ttgcactgag tctagtttcc tttgaagctg ttcgttcaat
     1021 ggtgctgtct gcttctcgat ttctgtatag atcatcgttg gcaatagttc gttgatctcg
     1081 ttctttagat ttaacccagt cactaattgc tctaaatggc tctgtaatcg ctgttttaag
     1141 ctgctctcca agccttgcaa taaatcctct gatagcttcg gattctgtag tttcttgatg
     1201 atattgatca gctcgttttc tttctcgttc aactggttgc agtgcttcac tagtttgctg
     1261 ttgtagttct ctaattcgtc tatggttctg ttctgtgcct gaatgagctg caaggcttgc
     1321 tcttgctgct tctgttgctc tttgagctgc tgctgttgct gctcgataat ctgcaataac
     1381 tgtgcttcga gattcattta attggtttcc tgcactattc gcatgttgtt gctgtactgc
     1441 tgcgctttcg ggtcgatctg gacgcaccag ttccattttt tctctgtccc acacttctga
     1501 atattcaggc tcgccccttg gctttttaac cactggactt tgttctccat ctctgtgatg
     1561 tcgctgtatt tgctcttgct gacgtacccc agacaaatac tgctgataag cgttatacat
     1621 gccagtgaaa tcaaggctat attcgatgta ataagggttt tctttaattt ggctgcttgg
     1681 gctgtttctt ggctcaagct gttcaactgc tgcaagttcg cttgtagctg tttgatctgc
     1741 tgttgttgtt cttgcaatag ggctgtcatg ccttgttgct gctgcttctt ggtttgagct
     1801 gtagtctggt cgatctgctg caatatctcg ttcgttgctt cgctctgttt ctgctgtata
     1861 tacgttttgg tgctgtctat actgctgctc aagctctgtg cttcgctttg cactgtattc
     1921 cgcatatctt tgaatatctg actgatttcg ctgtttagct tctcgtctat atcgttctga
     1981 tgctgcgata gtaagttgtt ggctcgtttc tgtaagtcta aaatcttgct catagattgc
     2041 acctgtaagc cttatcgggc gggctttttc gtcatcattc ggatttttaa tggataggct
     2101 tttcttggtt tctctggtga cttcaagacc aagatccaat agccattttt tcatgctctg
     2161 ccgatctgta attagtccac cttcaacggc tctcgttgct tcaatatcaa gcgtttcctt
     2221 gatttcatta acagttttag gcaagttttt tgccgttgta acggcttggc gatagagggg
     2281 gtcatctgca tcatgcagtt gatattcata gtttgtaatc ttcttaaaca gatcaatacg
     2341 aggacgatca gctttatcat aataaggctg taaacgttgc cctgtaagga tttcaacatt
     2401 agggattagg aagttaagct ctaatcttcg ctcccctgtt tcttcgttta atttgtcttg
     2461 atgctcaatc cacaagacac ggtactggct ctgatccatg ccagggaata aggcttgctc
     2521 aaaatcctgc atgatttttt gtttatcctg gtcagataaa tcatgctcat aaaaacttag
     2581 gcagccggat gtatattttt ttgcataagg tgacgtatca attaatcctg atacttcgga
     2641 aacatcgccc tgtaggactt ttgcataatc tcgatcacgg tctttaccaa gtaaataatc
     2701 cagtggacct ttacttaaac cgttcccata tctaaagaat tgaacaatca ttttttcaat
     2761 tcctttaagg tctgatcaat ccctattaac aggtgcaaaa gccttgcagc atcaagggga
     2821 cgactgcttg caatatcagt atttattgcc tttgcaatct gattgatgtt gcccccaatt
     2881 cgggatagct caagcaaaaa gtcacggtct aattttgtgt agtggttttc ctgattcttt
     2941 tttttggcgt tgccttgggt tgcatcaatt tgggcaagcg aagtatccct tagatatttg
     3001 gcaatattta ggatattggc atctttgatt ttctgtacct ctgcatcaga aaatcggact
     3061 ttgtaaacct tattacgttt gatcgctgtc atgcttttgc ctttgctttt gggggttttc
     3121 gggggcttgc ccctgaacaa ggtcatggaa cacatctgcg tagcagaggg gtctccatgt
     3181 atccttgcgt ttcattacga tagcagaccc ctagacgtaa ttcaattaag attaagcagg
     3241 tcatagcaag actatggaca atcgcaagcg accgtaaaac ctgatctgtg cctaaacact
     3301 atgcacaact ccgcataacg ataaagccgt tatacgttga gttgcacaca ggtttagatc
     3361 acagggtttt actttgccca cagccgttgc tcattccctt taataataat ttttaaaaat
     3421 gaaatgggaa aaaagcaccc tagggggctg atatctgatt taggcttgca ctttccgctt
     3481 ttttatccac gttctgattg ttctatccga aacattcaat tgtagggcta tatctttctg
     3541 ctttatgccc tgtatatgca gttttaaggc ttcctgacgc atatcactgt attgctgtga
     3601 cctagcctta ccgccatgac tggaatcact gcttagaccg cctttacgcc ctttgacagt
     3661 ctgacgctcg ataaatttgg catgtgcttc tgtgtgattg cgccataccc aacgagcaat
     3721 agacttagcg gttgccttta cctctgaata tggcaatggc tctaaaaacg caccatttgc
     3781 gtttatagcg acttttagca cttccgcata ccaactatca ggcgaccccg aaataaacgc
     3841 tctaatcgct tcatacgcc
//
