LOCUS       NZ_CP061644             6162 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS40 plasmid pAS40-4, complete
            sequence.
ACCESSION   NZ_CP061644
VERSION     NZ_CP061644.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092224
            Assembly: GCF_014721815.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,365
            CDSs (total)                      :: 4,268
            Genes (coding)                    :: 4,118
            CDSs (with protein)               :: 4,118
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 76 of 150
            Pseudo Genes (incomplete)         :: 73 of 150
            Pseudo Genes (internal stop)      :: 21 of 150
            Pseudo Genes (multiple problems)  :: 16 of 150
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061644.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS40"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS40-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5980..6162,1..1632)
                     /locus_tag="IC777_RS21645"
                     /old_locus_tag="IC777_21630"
     CDS             join(5980..6162,1..1632)
                     /locus_tag="IC777_RS21645"
                     /old_locus_tag="IC777_21630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1741..2226
                     /locus_tag="IC777_RS21650"
                     /old_locus_tag="IC777_21635"
     CDS             1741..2226
                     /locus_tag="IC777_RS21650"
                     /old_locus_tag="IC777_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            2359..3027
                     /locus_tag="IC777_RS21655"
                     /old_locus_tag="IC777_21640"
     CDS             2359..3027
                     /locus_tag="IC777_RS21655"
                     /old_locus_tag="IC777_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3270..3803)
                     /gene="aadB"
                     /locus_tag="IC777_RS21660"
                     /old_locus_tag="IC777_21645"
     CDS             complement(3270..3803)
                     /gene="aadB"
                     /locus_tag="IC777_RS21660"
                     /old_locus_tag="IC777_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3894..4580)
                     /locus_tag="IC777_RS21665"
                     /old_locus_tag="IC777_21650"
     CDS             complement(3894..4580)
                     /locus_tag="IC777_RS21665"
                     /old_locus_tag="IC777_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5583..5990
                     /locus_tag="IC777_RS21670"
                     /old_locus_tag="IC777_21655"
     CDS             5583..5990
                     /locus_tag="IC777_RS21670"
                     /old_locus_tag="IC777_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(3274..3803)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(3271..3815)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          2..1629
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5981..6162
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="1.52e-32"
ORIGIN
        1 gagcatgatt tatctgacca ggataaacag aaaatcatgc aaaattttga ggaatgttta
       61 ttcccgggac tagataaaga ccaatatcaa attttatggg tacagcacca agacaaaatt
      121 aatcaggaca caggtgagac aaggcttgag cttaattttg tgattcctaa tgttgagctt
      181 tcaacaggca aacgcctaca gccattctat gcgccagtcg atttagatcg agtcgatcta
      241 tttaagcaga tcactaatac cgaacattca ctgtatgacc ccgatgatcc tgagcatcga
      301 cagctatttt taaataaaaa gaacctaccc aaagatatta aggattttaa agcgcagtta
      361 catcaacgtg tttaccgagc ggttgccaat ggtgatgttg ccgatcggca agaattggtg
      421 caatggcttg aatcgaatca aatcaatgtc actcgacaag taaaaaactc catatccatt
      481 gaaaacccat atgagggcgc aaaacgtccc atccgtttag agggcgagat ttatgagcaa
      541 ggctttagag ctactggcga atatagacaa gaagtacaac aacgaattga agaatacaga
      601 ggaacaactt ctgagcggta tcgaacaaac gtcacagact atcagagaca gcttgaacat
      661 aagagccaat atcactgcga ccgctatcca acagtcggac gagaaaatag cccagagcat
      721 tcaaaacaac gcccagacgg tcgagaagca gttaagcctg tatcaaacct tgctgcaatc
      781 gaaattgagc catttaacgc aattaaacga gcaaatacag agccaagaac aacaagccca
      841 gaatcaagcg gtacagaaaa aacttaccac tttgaatatg ggactgattt cagtagcagt
      901 tattttgctt atagcaattt tctcgcttgg tctcgtcacc aaaaacaagt acagcgagat
      961 gcagacgcta aataccgtga tcaacgagaa gcaaatgagg gtcgaccatt tgaaccgatc
     1021 gggaggcagt ttgacgatca gcacatgcaa gcgaccagac agcaaagcac agcgcctatg
     1081 tattcagatc aacaagaacg caggcgaatg gcagaacggc tacatgattc caatggggta
     1141 ttaaacgatg accgaattag aaacacaatt attgagaatc atcgaagaac aacagcaaca
     1201 attaatgcaa caacaagtgc aattgcagaa gcaacagcaa gctttagaca caatgcaaac
     1261 caaaattatc caagccttgt cagcacaatc aaagacaatc aagagcgatc agcaaagctt
     1321 acagcaagca cagaaatcat tagcgacaat gcagaagcaa tttcacgagc tagaacgaag
     1381 tacagcaagc tataccgagc agatcaatgg caaagtccga gagatgagcc ttatcacagc
     1441 caacaacgaa gaaattctgg agagaatctc aaccacacag cattctgtct cgtcagaagt
     1501 agccaaagcg cagcagaatt tgctcgaaac atccgagata ttgaaaaaaa tctcgtccag
     1561 atgaatgagc gtaaacaaga gatagaaaaa cctcgaccta agcaagatcg aggtatggat
     1621 tttgggatgt aattattttc tagtatgttg gtgtttattt cttagtatta gcttgtacta
     1681 atgtttaaat ttagctatat tgaatgtagt ataggcttgt ttatatattt tggtggtgtt
     1741 atgaataata actgtgtagt agtactaact gctcgtggaa agactagaat tttaaaagaa
     1801 ggtggtagtc agtcttggag actaaatgct cataatgcag gaaagtgcga ttatctagtt
     1861 tgtgtgcaaa ataggaaaca atcttgggga gaaccagagg ctgatcatca cactgcattt
     1921 ttagttggca aaatatctag tatcagccaa tctcaagaag aagatgcaga tggtagatgg
     1981 ataataaata tctccgaata tgctgatata tctattccca atatgtggga tggtaatcgg
     2041 aatccagttt cttatagttc attagaagag atgaatatcg atttgaatga gctgaaattt
     2101 accaaattaa atgaagataa ttcttctaaa acactggata aaatatctat tcctgaagct
     2161 aagagactat tagctaatta ttttgatgtc cctcaagaaa atatttcaat tacaatcaat
     2221 atgtaagctt ttaattgacc aaaaaaagaa aaaagccttt cacgttaatg aaaggctttt
     2281 ttgaatctta gcgcttggga gggaagatct tagatccgca gtctctcaaa cttacaacta
     2341 tatattaggg tatatacggt ggaattgtca aatgatgcag tgcttaagat agagcaggct
     2401 ttatctatac cacgcttatc taagtatgaa aatttctata aagataaagg tgaaccatat
     2461 gaaaaatctg atgtattgat gctttatgaa cgtaatttaa taatttccaa taagtttttt
     2521 tacttattaa attattttga agttgttctg agaaatgctg ttgttcaggc aatagaaata
     2581 tcttttaggt gtaatgaaac aaatagttgg catgaaaatg aagcttttat tcgaagcctt
     2641 agccgtagag ggaggtatag ccctaaaagt atgtttgata gtgctaaaga gaagttccca
     2701 gattcgccta gtaaaatgat ccctgagctt aaatttgtat tctggcaaaa gatgcttatg
     2761 gctaattatg aagaacgtat ttggcaggga tgttttaatt ctatttttcc taatgcaaca
     2821 gttgaaaaca ggcaaatttt ttacgattgg acagatcaat tacgtgaatt aagaaacagg
     2881 attgctcatc atgaaccgat tatttttaac cgtaatctag aaaatgattt agaggtttta
     2941 acaaaattta tttactgtcg ttgtattaat acatacgatt ttatcgagca atctgcatta
     3001 gatctaaaag aatatttaga tttttaagat aagaatttca ccccaaacaa tcgaaaggtc
     3061 agactatttt gcaaatgttg aattaacaag gttgcattta tcgtgaaacg ctttcgcgtt
     3121 tttcgtgcgc cgcttcaaaa aaaataaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagt
     3181 agtaaagcag taaatccgat aacggtagca gtgtttccta acacctgagt taagccgcgc
     3241 cgcaaagcgg cgtcggcttg gacgaattgt taggccgcat atcgcgacct gaaagcggca
     3301 cgcaagacct caaccttttc cgccccgagt gaggtgcatg cgaacctgta ggactctatg
     3361 tgctttgtag gccagtccac tggtggtact tcatcggcat agtaaaagta atcccagatg
     3421 atcgcctccc agctgttaca acggactggc cgcccggcga tgacaccctc agccgcctct
     3481 gggcacgagc cctgcggagc ctccgcgatt tcatacgctt cgtctgccca ccaagcaggt
     3541 tcgcagtcaa gtaactcatc cccgatctcc gctaagaatc catagtccaa ctcctccgtg
     3601 acgcgcccgc cgagcatttc aactattgcc tcgagctcgc cgcgcctctc gccgggaaaa
     3661 gtcagatcaa tatcatcgtg cttgcgtgtt acacgcccta gccgtgcatc gatcgcccag
     3721 cccccaccga tccagagcgg cagatttcgc tcatctgccg cagctagaat ttggtgtatc
     3781 aatgcgacct gcgttgtgtc catgcggcct aacatagtaa gccctcccag aatgtcagta
     3841 attagactat taattgaatt gttacatcca atagtgagct cccagtgatt gtcttaacta
     3901 tccaaccttt cgattggatg gggcagagca ccattattct gtttgaatgc ggtgttagtg
     3961 tcaactaggt tctttatttc ttcaaggagt ttgaaagtgc taagtatttt tactttactt
     4021 tgttcctcaa tataaatttt tttaagacca tttagcagct tgtatttgga tattttggat
     4081 aggagaatga ataaggttgc ggtattgatt cttccccctt ttagatgaag taaacggttt
     4141 ttttgcagta ttggaatatt tctatattct gttagttgat cttttaagtc atcaggaatt
     4201 ttcatttcgt aaaaattata ttttttagaa cagtctaata tcgagtgatt taggctttct
     4261 gtatttgtat taataaaaat gcatttttca aatgtgcaat ttttaaagcg acaatctcta
     4321 aaaatagcag cataaaatac gcactttttg aagtgaacat tcgtaaacct attcttattt
     4381 aagattactc ctgaaaaatc agataaaaca atttttatat ttttgaatga acatgatcgt
     4441 aggcgggatc ctctcaaatt aacattaaaa aatgttttat tagagaaacg tactttagaa
     4501 gaaatagatt tggctaggag gctatttgaa tacttacgtt tttcgatgtg cttgttatga
     4561 aatctaggaa gttttgacat taatagtcca aagaatagga gtttcagctt aattcttagg
     4621 tgtaatcaaa aaaaataatg gacaggtctc ttgcgaaatc tgtccattag ttttaaattc
     4681 gatgggtaca gttatttgat gcttgcaaca tcaattaacg aaagatcggc agagcttgca
     4741 actcatactg atattcacct taaaggctag taccactatc tattttaaag aattagaaca
     4801 gtttgacaag cctcaatttg aagtccttcg ttaattcagc agtttaaatc atcaaataag
     4861 cacctaacgg tcgagcttta tctacttctg tgcgcctttt ttaaggaaga tatgcgttgc
     4921 atgtggaggg aataagtgtc actggtgcat cgtagcgata gtcgcaaggc taaaccgctt
     4981 acaggggatc tcacataggc ggagcgttgg aagtagttgt ataggttggt taagttgcat
     5041 gttggagtaa tcctcgcaga gataccacca tacttcgaca gggagcgcgt gggagaaggt
     5101 gcaaaacctt tagtccttgt ctgcatggtc agcaaaacca tgtgcggata cgaccgatgc
     5161 gtggctccgt cagcccaatt acaggttaat cggcaatact taagaggata tttgattttt
     5221 ttaaatcaaa gcctcttagg gaaagtattg ccgaaactct ctcaacgttc accatcagcc
     5281 cgagcaaagc gagggatgtg gggacggtga gaaagagttg agcgcatttg tttttacaag
     5341 attcatgcca atacctacta gagtttatat accgtgtcat ttgattagtt tttattgaat
     5401 ttcactcgtc catttttaaa taagcaagga tacacagcta cccctattta acttcgttaa
     5461 acagtgatag tctgtgacct tgatcagggg cttcaccccc gatacccccg aaaagcttta
     5521 aacagaaaaa tcaaagattt tccaatttaa agcttacaaa agccaaagca gggtagattg
     5581 aaatgtcagg acaggaacaa gaaaacaaac agagcaaagc agttcgagaa cgaaccttta
     5641 aagtcagact ttcagaatca gaatatcaat tgctcgattc caataatcct tatggttcga
     5701 ttgcaaagtt gcttagacaa gcagcagtag agaaagcgac taaaattcat aattccaaag
     5761 cagaagatga ccaaaaaatt aaagcaacaa aggttgttga atcttcgtat agcaagacag
     5821 agcgagcatt aattttagag cttggtcgga ttggcaacaa tgtgaatcag attgctaaag
     5881 cagtgaatac cgatattgca ggggtaggta cttttgacaa agtgaagtta cttcatttgt
     5941 tgatttccat agatcagcag cttcgggagt tacgtccaga tgatcgttga tttctttaga
     6001 catggttcag gtttatcaaa aggctgtctc gattatttgt taggtgaaga tcgagagcgt
     6061 gaacatgctc aagtgctcaa tggtgatgta gaactgacag cacaactcat tgatagcagt
     6121 ccttttgcca aaaaatatac cagtggttgt ttgtcctttt at
//
