LOCUS       NZ_CP061638             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS41 plasmid pAS41-4, complete
            sequence.
ACCESSION   NZ_CP061638
VERSION     NZ_CP061638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092225
            Assembly: GCF_014721575.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:22:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,196
            Genes (coding)                    :: 4,043
            CDSs (with protein)               :: 4,043
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 78 of 153
            Pseudo Genes (incomplete)         :: 73 of 153
            Pseudo Genes (internal stop)      :: 22 of 153
            Pseudo Genes (multiple problems)  :: 16 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS41"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS41-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            464..871
                     /locus_tag="IC778_RS21300"
                     /old_locus_tag="IC778_21285"
     CDS             464..871
                     /locus_tag="IC778_RS21300"
                     /old_locus_tag="IC778_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            861..2675
                     /locus_tag="IC778_RS21305"
                     /old_locus_tag="IC778_21290"
     CDS             861..2675
                     /locus_tag="IC778_RS21305"
                     /old_locus_tag="IC778_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2784..3269
                     /locus_tag="IC778_RS21310"
                     /old_locus_tag="IC778_21295"
     CDS             2784..3269
                     /locus_tag="IC778_RS21310"
                     /old_locus_tag="IC778_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            3402..4070
                     /locus_tag="IC778_RS21315"
                     /old_locus_tag="IC778_21300"
     CDS             3402..4070
                     /locus_tag="IC778_RS21315"
                     /old_locus_tag="IC778_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(4229..4762)
                     /gene="aadB"
                     /locus_tag="IC778_RS21320"
                     /old_locus_tag="IC778_21305"
     CDS             complement(4229..4762)
                     /gene="aadB"
                     /locus_tag="IC778_RS21320"
                     /old_locus_tag="IC778_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(4853..5539)
                     /locus_tag="IC778_RS21325"
                     /old_locus_tag="IC778_21310"
     CDS             complement(4853..5539)
                     /locus_tag="IC778_RS21325"
                     /old_locus_tag="IC778_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     ARG             complement(4233..4762)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(4230..4774)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          862..2672
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctgcatggt cagcaaaacc atgtgcggat acgaccgatg cgtggctccg tcagcccaat
       61 tacaggttaa tcggcaatac ttaagaggat atttgatttt tttaaatcaa agcctcttag
      121 ggaaagtatt gccgaaactc tctcaacgtt caccatcagc ccgagcaaag cgagggatgt
      181 ggggacggtg agaaagagtt gagcgcattt gtttttacaa gattcatgcc aatacctact
      241 agagtttata taccgtgtca tttgattagt ttttattgaa tttcactcgt ccatttttaa
      301 ataagcaagg atacacagct acccctattt aacttcgtta aacagtgata gtctgtgacc
      361 ttgatcaggg gcttcacccc cgataccccc gaaaagcttt aaacagaaaa atcaaagatt
      421 ttccaattta aagcttacaa aagccaaagc agggtagatt gaaatgtcag gacaggaaca
      481 agaaaacaaa cagagcaaag cagttcgaga acgaaccttt aaagtcagac tttcagaatc
      541 agaatatcaa ttgctcgatt ccaataatcc ttatggttcg attgcaaagt tgcttagaca
      601 agcagcagta gagaaagcga ctaaaattca taattccaaa gcagaagatg accaaaaaat
      661 taaagcaaca aaggttgttg aatcttcgta tagcaagaca gagcgagcat taattttaga
      721 gcttggtcgg attggcaaca atgtgaatca gattgctaaa gcagtgaata ccgatattgc
      781 aggggtaggt acttttgaca aagtgaagtt acttcatttg ttgatttcca tagatcagca
      841 gcttcgggag ttacgtccag atgatcgttg atttctttag acatggttca ggtttatcaa
      901 aaggctgtct cgattatttg ttaggtgaag atcgagagcg tgaacatgct caagtgctca
      961 atggtgatgt agaactgaca gcacaactca ttgatagcag tccttttgcc aaaaaatata
     1021 ccagtggttg tttgtccttt tatgagcatg atttatctga ccaggataaa cagaaaatca
     1081 tgcaaaattt tgaggaatgt ttattcccgg gactagataa agaccaatat caaattttat
     1141 gggtacagca ccaagacaaa attaatcagg acacaggtga gacaaggctt gagcttaatt
     1201 ttgtgattcc taatgttgag ctttcaacag gcaaacgcct acagccattc tatgcgccag
     1261 tcgatttaga tcgagtcgat ctatttaagc agatcactaa taccgaacat tcactgtatg
     1321 accccgatga tcctgagcat cgacagctat ttttaaataa aaagaaccta cccaaagata
     1381 ttaaggattt taaagcgcag ttacatcaac gtgtttaccg agcggttgcc aatggtgatg
     1441 ttgccgatcg gcaagaattg gtgcaatggc ttgaatcgaa tcaaatcaat gtcactcgac
     1501 aagtaaaaaa ctccatatcc attgaaaacc catatgaggg cgcaaaacgt cccatccgtt
     1561 tagagggcga gatttatgag caaggcttta gagctactgg cgaatataga caagaagtac
     1621 aacaacgaat tgaagaatac agaggaacaa cttctgagcg gtatcgaaca aacgtcacag
     1681 actatcagag acagcttgaa cataagagcc aatatcactg cgaccgctat ccaacagtcg
     1741 gacgagaaaa tagcccagag cattcaaaac aacgcccaga cggtcgagaa gcagttaagc
     1801 ctgtatcaaa ccttgctgca atcgaaattg agccatttaa cgcaattaaa cgagcaaata
     1861 cagagccaag aacaacaagc ccagaatcaa gcggtacaga aaaaacttac cactttgaat
     1921 atgggactga tttcagtagc agttattttg cttatagcaa ttttctcgct tggtctcgtc
     1981 accaaaaaca agtacagcga gatgcagacg ctaaataccg tgatcaacga gaagcaaatg
     2041 agggtcgacc atttgaaccg atcgggaggc agtttgacga tcagcacatg caagcgacca
     2101 gacagcaaag cacagcgcct atgtattcag atcaacaaga acgcaggcga atggcagaac
     2161 ggctacatga ttccaatggg gtattaaacg atgaccgaat tagaaacaca attattgaga
     2221 atcatcgaag aacaacagca acaattaatg caacaacaag tgcaattgca gaagcaacag
     2281 caagctttag acacaatgca aaccaaaatt atccaagcct tgtcagcaca atcaaagaca
     2341 atcaagagcg atcagcaaag cttacagcaa gcacagaaat cattagcgac aatgcagaag
     2401 caatttcacg agctagaacg aagtacagca agctataccg agcagatcaa tggcaaagtc
     2461 cgagagatga gccttatcac agccaacaac gaagaaattc tggagagaat ctcaaccaca
     2521 cagcattctg tctcgtcaga agtagccaaa gcgcagcaga atttgctcga aacatccgag
     2581 atattgaaaa aaatctcgtc cagatgaatg agcgtaaaca agagatagaa aaacctcgac
     2641 ctaagcaaga tcgaggtatg gattttggga tgtaattatt ttctagtatg ttggtgttta
     2701 tttcttagta ttagcttgta ctaatgttta aatttagcta tattgaatgt agtataggct
     2761 tgtttatata ttttggtggt gttatgaata ataactgtgt agtagtacta actgctcgtg
     2821 gaaagactag aattttaaaa gaaggtggta gtcagtcttg gagactaaat gctcataatg
     2881 caggaaagtg cgattatcta gtttgtgtgc aaaataggaa acaatcttgg ggagaaccag
     2941 aggctgatca tcacactgca tttttagttg gcaaaatatc tagtatcagc caatctcaag
     3001 aagaagatgc agatggtaga tggataataa atatctccga atatgctgat atatctattc
     3061 ccaatatgtg ggatggtaat cggaatccag tttcttatag ttcattagaa gagatgaata
     3121 tcgatttgaa tgagctgaaa tttaccaaat taaatgaaga taattcttct aaaacactgg
     3181 ataaaatatc tattcctgaa gctaagagac tattagctaa ttattttgat gtccctcaag
     3241 aaaatatttc aattacaatc aatatgtaag cttttaattg accaaaaaaa gaaaaaagcc
     3301 tttcacgtta atgaaaggct tttttgaatc ttagcgcttg ggagggaaga tcttagatcc
     3361 gcagtctctc aaacttacaa ctatatatta gggtatatac ggtggaattg tcaaatgatg
     3421 cagtgcttaa gatagagcag gctttatcta taccacgctt atctaagtat gaaaatttct
     3481 ataaagataa aggtgaacca tatgaaaaat ctgatgtatt gatgctttat gaacgtaatt
     3541 taataatttc caataagttt ttttacttat taaattattt tgaagttgtt ctgagaaatg
     3601 ctgttgttca ggcaatagaa atatctttta ggtgtaatga aacaaatagt tggcatgaaa
     3661 atgaagcttt tattcgaagc cttagccgta gagggaggta tagccctaaa agtatgtttg
     3721 atagtgctaa agagaagttc ccagattcgc ctagtaaaat gatccctgag cttaaatttg
     3781 tattctggca aaagatgctt atggctaatt atgaagaacg tatttggcag ggatgtttta
     3841 attctatttt tcctaatgca acagttgaaa acaggcaaat tttttacgat tggacagatc
     3901 aattacgtga attaagaaac aggattgctc atcatgaacc gattattttt aaccgtaatc
     3961 tagaaaatga tttagaggtt ttaacaaaat ttatttactg tcgttgtatt aatacatacg
     4021 attttatcga gcaatctgca ttagatctaa aagaatattt agatttttaa gataagaatt
     4081 tcaccccaaa caatcgaaag gtcagactat tttgcaaatg ttgaattaac aaggttagta
     4141 gtaaagcagt aaatccgata acggtagcag tgtttcctaa cacctgagtt aagccgcgcc
     4201 gcaaagcggc gtcggcttgg acgaattgtt aggccgcata tcgcgacctg aaagcggcac
     4261 gcaagacctc aaccttttcc gccccgagtg aggtgcatgc gaacctgtag gactctatgt
     4321 gctttgtagg ccagtccact ggtggtactt catcggcata gtaaaagtaa tcccagatga
     4381 tcgcctccca gctgttacaa cggactggcc gcccggcgat gacaccctca gccgcctctg
     4441 ggcacgagcc ctgcggagcc tccgcgattt catacgcttc gtctgcccac caagcaggtt
     4501 cgcagtcaag taactcatcc ccgatctccg ctaagaatcc atagtccaac tcctccgtga
     4561 cgcgcccgcc gagcatttca actattgcct cgagctcgcc gcgcctctcg ccgggaaaag
     4621 tcagatcaat atcatcgtgc ttgcgtgtta cacgccctag ccgtgcatcg atcgcccagc
     4681 ccccaccgat ccagagcggc agatttcgct catctgccgc agctagaatt tggtgtatca
     4741 atgcgacctg cgttgtgtcc atgcggccta acatagtaag ccctcccaga atgtcagtaa
     4801 ttagactatt aattgaattg ttacatccaa tagtgagctc ccagtgattg tcttaactat
     4861 ccaacctttc gattggatgg ggcagagcac cattattctg tttgaatgcg gtgttagtgt
     4921 caactaggtt ctttatttct tcaaggagtt tgaaagtgct aagtattttt actttacttt
     4981 gttcctcaat ataaattttt ttaagaccat ttagcagctt gtatttggat attttggata
     5041 ggagaatgaa taaggttgcg gtattgattc ttcccccttt tagatgaagt aaacggtttt
     5101 tttgcagtat tggaatattt ctatattctg ttagttgatc ttttaagtca tcaggaattt
     5161 tcatttcgta aaaattatat tttttagaac agtctaatat cgagtgattt aggctttctg
     5221 tatttgtatt aataaaaatg catttttcaa atgtgcaatt tttaaagcga caatctctaa
     5281 aaatagcagc ataaaatacg cactttttga agtgaacatt cgtaaaccta ttcttattta
     5341 agattactcc tgaaaaatca gataaaacaa tttttatatt tttgaatgaa catgatcgta
     5401 ggcgggatcc tctcaaatta acattaaaaa atgttttatt agagaaacgt actttagaag
     5461 aaatagattt ggctaggagg ctatttgaat acttacgttt ttcgatgtgc ttgttatgaa
     5521 atctaggaag ttttgacatt aatagtccaa agaataggag tttcagctta attcttaggt
     5581 gtaatcaaaa aaaataatgg acaggtctct tgcgaaatct gtccattagt tttaaattcg
     5641 atgggtacag ttatttgatg cttgcaacat caattaacga aagatcggca gagcttgcaa
     5701 ctcatactga tattcacctt aaaggctagt accactatct attttaaaga attagaacag
     5761 tttgacaagc ctcaatttga agtccttcgt taattcagca gtttaaatca tcaaataagc
     5821 acctaacggt cgagctttat ctacttctgt gcgccttttt taaggaagat atgcgttgca
     5881 tgtggaggga ataagtgtca ctggtgcatc gtagcgatag tcgcaaggct aaaccgctta
     5941 caggggatct cacataggcg gagcgttgga agtagttgta taggttggtt aagttgcatg
     6001 ttggagtaat cctcgcagag ataccaccat acttcgacag ggagcgcgtg ggagaaggtg
     6061 caaaaccttt agtccttg
//
