LOCUS       NZ_CP061633             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS47 plasmid pAS47-4, complete
            sequence.
ACCESSION   NZ_CP061633
VERSION     NZ_CP061633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092227
            Assembly: GCF_014721415.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:20:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,260
            Genes (coding)                    :: 4,105
            CDSs (with protein)               :: 4,105
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 71 of 155
            Pseudo Genes (internal stop)      :: 23 of 155
            Pseudo Genes (multiple problems)  :: 18 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061633.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS47"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS47-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(5720..6078,1..127))
                     /locus_tag="IC780_RS21620"
                     /old_locus_tag="IC780_21605"
     CDS             complement(join(5720..6078,1..127))
                     /locus_tag="IC780_RS21620"
                     /old_locus_tag="IC780_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(236..2050)
                     /locus_tag="IC780_RS21625"
                     /old_locus_tag="IC780_21610"
     CDS             complement(236..2050)
                     /locus_tag="IC780_RS21625"
                     /old_locus_tag="IC780_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2040..2447)
                     /locus_tag="IC780_RS21630"
                     /old_locus_tag="IC780_21615"
     CDS             complement(2040..2447)
                     /locus_tag="IC780_RS21630"
                     /old_locus_tag="IC780_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3450..4136
                     /locus_tag="IC780_RS21635"
                     /old_locus_tag="IC780_21620"
     CDS             3450..4136
                     /locus_tag="IC780_RS21635"
                     /old_locus_tag="IC780_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            4227..4760
                     /gene="aadB"
                     /locus_tag="IC780_RS21640"
                     /old_locus_tag="IC780_21625"
     CDS             4227..4760
                     /gene="aadB"
                     /locus_tag="IC780_RS21640"
                     /old_locus_tag="IC780_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(4919..5587)
                     /locus_tag="IC780_RS21645"
                     /old_locus_tag="IC780_21630"
     CDS             complement(4919..5587)
                     /locus_tag="IC780_RS21645"
                     /old_locus_tag="IC780_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     ARG             4228..4757
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             4216..4760
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(240..2050)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcacacaaac tagataatcg cactttcctg cattatgagc atttagtctc caagactgac
       61 taccaccttc ttttaaaatt ctagtctttc cacgagcagt tagtactact acacagttat
      121 tattcataac accaccaaaa tatataaaca agcctatact acattcaata tagctaaatt
      181 taaacattag tacaagctaa tactaagaaa taaacaccaa catactagaa aataattaca
      241 tcccaaaatc catacctcga tcttgcttag gtcgaggttt ttctatctct tgtttacgct
      301 cattcatctg gacgagattt ttttcaatat ctcggatgtt tcgagcaaat tctgctgcgc
      361 tttggctact tctgacgaga cagaatgctg tgtggttgag attctctcca gaatttcttc
      421 gttgttggct gtgataaggc tcatctctcg gactttgcca ttgatctgct cggtatagct
      481 tgctgtactt cgttctagct cgtgaaattg cttctgcatt gtcgctaatg atttctgtgc
      541 ttgctgtaag ctttgctgat cgctcttgat tgtctttgat tgtgctgaca aggcttggat
      601 aattttggtt tgcattgtgt ctaaagcttg ctgttgcttc tgcaattgca cttgttgttg
      661 cattaattgt tgctgttgtt cttcgatgat tctcaataat tgtgtttcta attcggtcat
      721 cgtttaatac cccattggaa tcatgtagcc gttctgccat tcgcctgcgt tcttgttgat
      781 ctgaatacat aggcgctgtg ctttgctgtc tggtcgcttg catgtgctga tcgtcaaact
      841 gcctcccgat cggttcaaat ggtcgaccct catttgcttc tcgttgatca cggtatttag
      901 cgtctgcatc tcgctgtact tgtttttggt gacgagacca agcgagaaaa ttgctataag
      961 caaaataact gctactgaaa tcagtcccat attcaaagtg gtaagttttt tctgtaccgc
     1021 ttgattctgg gcttgttgtt cttggctctg tatttgctcg tttaattgcg ttaaatggct
     1081 caatttcgat tgcagcaagg tttgatacag gcttaactgc ttctcgaccg tctgggcgtt
     1141 gttttgaatg ctctgggcta ttttctcgtc cgactgttgg atagcggtcg cagtgatatt
     1201 ggctcttatg ttcaagctgt ctctgatagt ctgtgacgtt tgttcgatac cgctcagaag
     1261 ttgttcctct gtattcttca attcgttgtt gtacttcttg tctatattcg ccagtagctc
     1321 taaagccttg ctcataaatc tcgccctcta aacggatggg acgttttgcg ccctcatatg
     1381 ggttttcaat ggatatggag ttttttactt gtcgagtgac attgatttga ttcgattcaa
     1441 gccattgcac caattcttgc cgatcggcaa catcaccatt ggcaaccgct cggtaaacac
     1501 gttgatgtaa ctgcgcttta aaatccttaa tatctttggg taggttcttt ttatttaaaa
     1561 atagctgtcg atgctcagga tcatcggggt catacagtga atgttcggta ttagtgatct
     1621 gcttaaatag atcgactcga tctaaatcga ctggcgcata gaatggctgt aggcgtttgc
     1681 ctgttgaaag ctcaacatta ggaatcacaa aattaagctc aagccttgtc tcacctgtgt
     1741 cctgattaat tttgtcttgg tgctgtaccc ataaaatttg atattggtct ttatctagtc
     1801 ccgggaataa acattcctca aaattttgca tgattttctg tttatcctgg tcagataaat
     1861 catgctcata aaaggacaaa caaccactgg tatatttttt ggcaaaagga ctgctatcaa
     1921 tgagttgtgc tgtcagttct acatcaccat tgagcacttg agcatgttca cgctctcgat
     1981 cttcacctaa caaataatcg agacagcctt ttgataaacc tgaaccatgt ctaaagaaat
     2041 caacgatcat ctggacgtaa ctcccgaagc tgctgatcta tggaaatcaa caaatgaagt
     2101 aacttcactt tgtcaaaagt acctacccct gcaatatcgg tattcactgc tttagcaatc
     2161 tgattcacat tgttgccaat ccgaccaagc tctaaaatta atgctcgctc tgtcttgcta
     2221 tacgaagatt caacaacctt tgttgcttta attttttggt catcttctgc tttggaatta
     2281 tgaattttag tcgctttctc tactgctgct tgtctaagca actttgcaat cgaaccataa
     2341 ggattattgg aatcgagcaa ttgatattct gattctgaaa gtctgacttt aaaggttcgt
     2401 tctcgaactg ctttgctctg tttgttttct tgttcctgtc ctgacatttc aatctaccct
     2461 gctttggctt ttgtaagctt taaattggaa aatctttgat ttttctgttt aaagcttttc
     2521 gggggtatcg ggggtgaagc ccctgatcaa ggtcacagac tatcactgtt taacgaagtt
     2581 aaataggggt agctgtgtat ccttgcttat ttaaaaatgg acgagtgaaa ttcaataaaa
     2641 actaatcaaa tgacacggta tataaactct agtaggtatt ggcatgaatc ttgtaaaaac
     2701 aaatgcgctc aactctttct caccgtcccc acatccctcg ctttgctcgg gctgatggtg
     2761 aacgttgaga gagtttcggc aatactttcc ctaagaggct ttgatttaaa aaaatcaaat
     2821 atcctcttaa gtattgccga ttaacctgta attgggctga cggagccacg catcggtcgt
     2881 atccgcacat ggttttgctg accatgcaga caaggactaa aggttttgca ccttctccca
     2941 cgcgctccct gtcgaagtat ggtggtatct ctgcgaggat tactccaaca tgcaacttaa
     3001 ccaacctata caactacttc caacgctccg cctatgtgag atcccctgta agcggtttag
     3061 ccttgcgact atcgctacga tgcaccagtg acacttattc cctccacatg caacgcatat
     3121 cttccttaaa aaaggcgcac agaagtagat aaagctcgac cgttaggtgc ttatttgatg
     3181 atttaaactg ctgaattaac gaaggacttc aaattgaggc ttgtcaaact gttctaattc
     3241 tttaaaatag atagtggtac tagcctttaa ggtgaatatc agtatgagtt gcaagctctg
     3301 ccgatctttc gttaattgat gttgcaagca tcaaataact gtacccatcg aatttaaaac
     3361 taatggacag atttcgcaag agacctgtcc attatttttt ttgattacac ctaagaatta
     3421 agctgaaact cctattcttt ggactattaa tgtcaaaact tcctagattt cataacaagc
     3481 acatcgaaaa acgtaagtat tcaaatagcc tcctagccaa atctatttct tctaaagtac
     3541 gtttctctaa taaaacattt tttaatgtta atttgagagg atcccgccta cgatcatgtt
     3601 cattcaaaaa tataaaaatt gttttatctg atttttcagg agtaatctta aataagaata
     3661 ggtttacgaa tgttcacttc aaaaagtgcg tattttatgc tgctattttt agagattgtc
     3721 gctttaaaaa ttgcacattt gaaaaatgca tttttattaa tacaaataca gaaagcctaa
     3781 atcactcgat attagactgt tctaaaaaat ataattttta cgaaatgaaa attcctgatg
     3841 acttaaaaga tcaactaaca gaatatagaa atattccaat actgcaaaaa aaccgtttac
     3901 ttcatctaaa agggggaaga atcaataccg caaccttatt cattctccta tccaaaatat
     3961 ccaaatacaa gctgctaaat ggtcttaaaa aaatttatat tgaggaacaa agtaaagtaa
     4021 aaatacttag cactttcaaa ctccttgaag aaataaagaa cctagttgac actaacaccg
     4081 cattcaaaca gaataatggt gctctgcccc atccaatcga aaggttggat agttaagaca
     4141 atcactggga gctcactatt ggatgtaaca attcaattaa tagtctaatt actgacattc
     4201 tgggagggct tactatgtta ggccgcatgg acacaacgca ggtcgcattg atacaccaaa
     4261 ttctagctgc ggcagatgag cgaaatctgc cgctctggat cggtgggggc tgggcgatcg
     4321 atgcacggct agggcgtgta acacgcaagc acgatgatat tgatctgact tttcccggcg
     4381 agaggcgcgg cgagctcgag gcaatagttg aaatgctcgg cgggcgcgtc acggaggagt
     4441 tggactatgg attcttagcg gagatcgggg atgagttact tgactgcgaa cctgcttggt
     4501 gggcagacga agcgtatgaa atcgcggagg ctccgcaggg ctcgtgccca gaggcggctg
     4561 agggtgtcat cgccgggcgg ccagtccgtt gtaacagctg ggaggcgatc atctgggatt
     4621 acttttacta tgccgatgaa gtaccaccag tggactggcc tacaaagcac atagagtcct
     4681 acaggttcgc atgcacctca ctcggggcgg aaaaggttga ggtcttgcgt gccgctttca
     4741 ggtcgcgata tgcggcctaa caattcgtcc aagccgacgc cgctttgcgg cgcggcttaa
     4801 ctcaggtgtt aggaaacact gctaccgtta tcggatttac tgctttacta ctaaccttgt
     4861 taattcaaca tttgcaaaat agtctgacct ttcgattgtt tggggtgaaa ttcttatctt
     4921 aaaaatctaa atattctttt agatctaatg cagattgctc gataaaatcg tatgtattaa
     4981 tacaacgaca gtaaataaat tttgttaaaa cctctaaatc attttctaga ttacggttaa
     5041 aaataatcgg ttcatgatga gcaatcctgt ttcttaattc acgtaattga tctgtccaat
     5101 cgtaaaaaat ttgcctgttt tcaactgttg cattaggaaa aatagaatta aaacatccct
     5161 gccaaatacg ttcttcataa ttagccataa gcatcttttg ccagaataca aatttaagct
     5221 cagggatcat tttactaggc gaatctggga acttctcttt agcactatca aacatacttt
     5281 tagggctata cctccctcta cggctaaggc ttcgaataaa agcttcattt tcatgccaac
     5341 tatttgtttc attacaccta aaagatattt ctattgcctg aacaacagca tttctcagaa
     5401 caacttcaaa ataatttaat aagtaaaaaa acttattgga aattattaaa ttacgttcat
     5461 aaagcatcaa tacatcagat ttttcatatg gttcaccttt atctttatag aaattttcat
     5521 acttagataa gcgtggtata gataaagcct gctctatctt aagcactgca tcatttgaca
     5581 attccaccgt atatacccta atatatagtt gtaagtttga gagactgcgg atctaagatc
     5641 ttccctccca agcgctaaga ttcaaaaaag cctttcatta acgtgaaagg cttttttctt
     5701 tttttggtca attaaaagct tacatattga ttgtaattga aatattttct tgagggacat
     5761 caaaataatt agctaatagt ctcttagctt caggaataga tattttatcc agtgttttag
     5821 aagaattatc ttcatttaat ttggtaaatt tcagctcatt caaatcgata ttcatctctt
     5881 ctaatgaact ataagaaact ggattccgat taccatccca catattggga atagatatat
     5941 cagcatattc ggagatattt attatccatc taccatctgc atcttcttct tgagattggc
     6001 tgatactaga tattttgcca actaaaaatg cagtgtgatg atcagcctct ggttctcccc
     6061 aagattgttt cctatttt
//
