LOCUS       NZ_CP061624             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS49 plasmid pAS49-4, complete
            sequence.
ACCESSION   NZ_CP061624
VERSION     NZ_CP061624.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092229
            Assembly: GCF_014721075.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:41:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,310
            Genes (coding)                    :: 4,156
            CDSs (with protein)               :: 4,156
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 82 of 154
            Pseudo Genes (incomplete)         :: 69 of 154
            Pseudo Genes (internal stop)      :: 26 of 154
            Pseudo Genes (multiple problems)  :: 19 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061624.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS49"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS49-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5638..6078,1..93)
                     /gene="aadB"
                     /locus_tag="IC782_RS21875"
                     /old_locus_tag="IC782_21860"
     CDS             join(5638..6078,1..93)
                     /gene="aadB"
                     /locus_tag="IC782_RS21875"
                     /old_locus_tag="IC782_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(252..920)
                     /locus_tag="IC782_RS21880"
                     /old_locus_tag="IC782_21865"
     CDS             complement(252..920)
                     /locus_tag="IC782_RS21880"
                     /old_locus_tag="IC782_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(1053..1538)
                     /locus_tag="IC782_RS21885"
                     /old_locus_tag="IC782_21870"
     CDS             complement(1053..1538)
                     /locus_tag="IC782_RS21885"
                     /old_locus_tag="IC782_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(1647..3461)
                     /locus_tag="IC782_RS21890"
                     /old_locus_tag="IC782_21875"
     CDS             complement(1647..3461)
                     /locus_tag="IC782_RS21890"
                     /old_locus_tag="IC782_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3451..3858)
                     /locus_tag="IC782_RS21895"
                     /old_locus_tag="IC782_21880"
     CDS             complement(3451..3858)
                     /locus_tag="IC782_RS21895"
                     /old_locus_tag="IC782_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4861..5547
                     /locus_tag="IC782_RS21900"
                     /old_locus_tag="IC782_21885"
     CDS             4861..5547
                     /locus_tag="IC782_RS21900"
                     /old_locus_tag="IC782_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     ARG             5627..6078
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.96"
                     /coverage="85.31"
     Typing          complement(1651..3461)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacatagagt cctacaggtt cgcatgcacc tcactcgggg cggaaaaggt tgaggtcttg
       61 cgtgccgctt tcaggtcgcg atatgcggcc taacaattcg tccaagccga cgccgctttg
      121 cggcgcggct taactcaggt gttaggaaac actgctaccg ttatcggatt tactgcttta
      181 ctactaacct tgttaattca acatttgcaa aatagtctga cctttcgatt gtttggggtg
      241 aaattcttat cttaaaaatc taaatattct tttagatcta atgcagattg ctcgataaaa
      301 tcgtatgtat taatacaacg acagtaaata aattttgtta aaacctctaa atcattttct
      361 agattacggt taaaaataat cggttcatga tgagcaatcc tgtttcttaa ttcacgtaat
      421 tgatctgtcc aatcgtaaaa aatttgcctg ttttcaactg ttgcattagg aaaaatagaa
      481 ttaaaacatc cctgccaaat acgttcttca taattagcca taagcatctt ttgccagaat
      541 acaaatttaa gctcagggat cattttacta ggcgaatctg ggaacttctc tttagcacta
      601 tcaaacatac ttttagggct atacctccct ctacggctaa ggcttcgaat aaaagcttca
      661 ttttcatgcc aactatttgt ttcattacac ctaaaagata tttctattgc ctgaacaaca
      721 gcatttctca gaacaacttc aaaataattt aataagtaaa aaaacttatt ggaaattatt
      781 aaattacgtt cataaagcat caatacatca gatttttcat atggttcacc tttatcttta
      841 tagaaatttt catacttaga taagcgtggt atagataaag cctgctctat cttaagcact
      901 gcatcatttg acaattccac cgtatatacc ctaatatata gttgtaagtt tgagagactg
      961 cggatctaag atcttccctc ccaagcgcta agattcaaaa aagcctttca ttaacgtgaa
     1021 aggctttttt ctttttttgg tcaattaaaa gcttacatat tgattgtaat tgaaatattt
     1081 tcttgaggga catcaaaata attagctaat agtctcttag cttcaggaat agatatttta
     1141 tccagtgttt tagaagaatt atcttcattt aatttggtaa atttcagctc attcaaatcg
     1201 atattcatct cttctaatga actataagaa actggattcc gattaccatc ccacatattg
     1261 ggaatagata tatcagcata ttcggagata tttattatcc atctaccatc tgcatcttct
     1321 tcttgagatt ggctgatact agatattttg ccaactaaaa atgcagtgtg atgatcagcc
     1381 tctggttctc cccaagattg tttcctattt tgcacacaaa ctagataatc gcactttcct
     1441 gcattatgag catttagtct ccaagactga ctaccacctt cttttaaaat tctagtcttt
     1501 ccacgagcag ttagtactac tacacagtta ttattcataa caccaccaaa atatataaac
     1561 aagcctatac tacattcaat atagctaaat ttaaacatta gtacaagcta atactaagaa
     1621 ataaacacca acatactaga aaataattac atcccaaaat ccatacctcg atcttgctta
     1681 ggtcgaggtt tttctatctc ttgtttacgc tcattcatct ggacgagatt tttttcaata
     1741 tctcggatgt ttcgagcaaa ttctgctgcg ctttggctac ttctgacgag acagaatgct
     1801 gtgtggttga gattctctcc agaatttctt cgttgttggc tgtgataagg ctcatctctc
     1861 ggactttgcc attgatctgc tcggtatagc ttgctgtact tcgttctagc tcgtgaaatt
     1921 gcttctgcat tgtcgctaat gatttctgtg cttgctgtaa gctttgctga tcgctcttga
     1981 ttgtctttga ttgtgctgac aaggcttgga taattttggt ttgcattgtg tctaaagctt
     2041 gctgttgctt ctgcaattgc acttgttgtt gcattaattg ttgctgttgt tcttcgatga
     2101 ttctcaataa ttgtgtttct aattcggtca tcgtttaata ccccattgga atcatgtagc
     2161 cgttctgcca ttcgcctgcg ttcttgttga tctgaataca taggcgctgt gctttgctgt
     2221 ctggtcgctt gcatgtgctg atcgtcaaac tgcctcccga tcggttcaaa tggtcgaccc
     2281 tcatttgctt ctcgttgatc acggtattta gcgtctgcat ctcgctgtac ttgtttttgg
     2341 tgacgagacc aagcgagaaa attgctataa gcaaaataac tgctactgaa atcagtccca
     2401 tattcaaagt ggtaagtttt ttctgtaccg cttgattctg ggcttgttgt tcttggctct
     2461 gtatttgctc gtttaattgc gttaaatggc tcaatttcga ttgcagcaag gtttgataca
     2521 ggcttaactg cttctcgacc gtctgggcgt tgttttgaat gctctgggct attttctcgt
     2581 ccgactgttg gatagcggtc gcagtgatat tggctcttat gttcaagctg tctctgatag
     2641 tctgtgacgt ttgttcgata ccgctcagaa gttgttcctc tgtattcttc aattcgttgt
     2701 tgtacttctt gtctatattc gccagtagct ctaaagcctt gctcataaat ctcgccctct
     2761 aaacggatgg gacgttttgc gccctcatat gggttttcaa tggatatgga gttttttact
     2821 tgtcgagtga cattgatttg attcgattca agccattgca ccaattcttg ccgatcggca
     2881 acatcaccat tggcaaccgc tcggtaaaca cgttgatgta actgcgcttt aaaatcctta
     2941 atatctttgg gtaggttctt tttatttaaa aatagctgtc gatgctcagg atcatcgggg
     3001 tcatacagtg aatgttcggt attagtgatc tgcttaaata gatcgactcg atctaaatcg
     3061 actggcgcat agaatggctg taggcgtttg cctgttgaaa gctcaacatt aggaatcaca
     3121 aaattaagct caagccttgt ctcacctgtg tcctgattaa ttttgtcttg gtgctgtacc
     3181 cataaaattt gatattggtc tttatctagt cccgggaata aacattcctc aaaattttgc
     3241 atgattttct gtttatcctg gtcagataaa tcatgctcat aaaaggacaa acaaccactg
     3301 gtatattttt tggcaaaagg actgctatca atgagttgtg ctgtcagttc tacatcacca
     3361 ttgagcactt gagcatgttc acgctctcga tcttcaccta acaaataatc gagacagcct
     3421 tttgataaac ctgaaccatg tctaaagaaa tcaacgatca tctggacgta actcccgaag
     3481 ctgctgatct atggaaatca acaaatgaag taacttcact ttgtcaaaag tacctacccc
     3541 tgcaatatcg gtattcactg ctttagcaat ctgattcaca ttgttgccaa tccgaccaag
     3601 ctctaaaatt aatgctcgct ctgtcttgct atacgaagat tcaacaacct ttgttgcttt
     3661 aattttttgg tcatcttctg ctttggaatt atgaatttta gtcgctttct ctactgctgc
     3721 ttgtctaagc aactttgcaa tcgaaccata aggattattg gaatcgagca attgatattc
     3781 tgattctgaa agtctgactt taaaggttcg ttctcgaact gctttgctct gtttgttttc
     3841 ttgttcctgt cctgacattt caatctaccc tgctttggct tttgtaagct ttaaattgga
     3901 aaatctttga tttttctgtt taaagctttt cgggggtatc gggggtgaag cccctgatca
     3961 aggtcacaga ctatcactgt ttaacgaagt taaatagggg tagctgtgta tccttgctta
     4021 tttaaaaatg gacgagtgaa attcaataaa aactaatcaa atgacacggt atataaactc
     4081 tagtaggtat tggcatgaat cttgtaaaaa caaatgcgct caactctttc tcaccgtccc
     4141 cacatccctc gctttgctcg ggctgatggt gaacgttgag agagtttcgg caatactttc
     4201 cctaagaggc tttgatttaa aaaaatcaaa tatcctctta agtattgccg attaacctgt
     4261 aattgggctg acggagccac gcatcggtcg tatccgcaca tggttttgct gaccatgcag
     4321 acaaggacta aaggttttgc accttctccc acgcgctccc tgtcgaagta tggtggtatc
     4381 tctgcgagga ttactccaac atgcaactta accaacctat acaactactt ccaacgctcc
     4441 gcctatgtga gatcccctgt aagcggttta gccttgcgac tatcgctacg atgcaccagt
     4501 gacacttatt ccctccacat gcaacgcata tcttccttaa aaaaggcgca cagaagtaga
     4561 taaagctcga ccgttaggtg cttatttgat gatttaaact gctgaattaa cgaaggactt
     4621 caaattgagg cttgtcaaac tgttctaatt ctttaaaata gatagtggta ctagccttta
     4681 aggtgaatat cagtatgagt tgcaagctct gccgatcttt cgttaattga tgttgcaagc
     4741 atcaaataac tgtacccatc gaatttaaaa ctaatggaca gatttcgcaa gagacctgtc
     4801 cattattttt tttgattaca cctaagaatt aagctgaaac tcctattctt tggactatta
     4861 atgtcaaaac ttcctagatt tcataacaag cacatcgaaa aacgtaagta ttcaaatagc
     4921 ctcctagcca aatctatttc ttctaaagta cgtttctcta ataaaacatt ttttaatgtt
     4981 aatttgagag gatcccgcct acgatcatgt tcattcaaaa atataaaaat tgttttatct
     5041 gatttttcag gagtaatctt aaataagaat aggtttacga atgttcactt caaaaagtgc
     5101 gtattttatg ctgctatttt tagagattgt cgctttaaaa attgcacatt tgaaaaatgc
     5161 atttttatta atacaaatac agaaagccta aatcactcga tattagactg ttctaaaaaa
     5221 tataattttt acgaaatgaa aattcctgat gacttaaaag atcaactaac agaatataga
     5281 aatattccaa tactgcaaaa aaaccgttta cttcatctaa aagggggaag aatcaatacc
     5341 gcaaccttat tcattctcct atccaaaata tccaaataca agctgctaaa tggtcttaaa
     5401 aaaatttata ttgaggaaca aagtaaagta aaaatactta gcactttcaa actccttgaa
     5461 gaaataaaga acctagttga cactaacacc gcattcaaac agaataatgg tgctctgccc
     5521 catccaatcg aaaggttgga tagttaagac aatcactggg agctcactat tggatgtaac
     5581 aattcaatta atagtctaat tactgacatt ctgggagggc ttactatgtt aggccgcatg
     5641 gacacaacgc aggtcgcatt gatacaccaa attctagctg cggcagatga gcgaaatctg
     5701 ccgctctgga tcggtggggg ctgggcgatc gatgcacggc tagggcgtgt aacacgcaag
     5761 cacgatgata ttgatctgac ttttcccggc gagaggcgcg gcgagctcga ggcaatagtt
     5821 gaaatgctcg gcgggcgcgt cacggaggag ttggactatg gattcttagc ggagatcggg
     5881 gatgagttac ttgactgcga acctgcttgg tgggcagacg aagcgtatga aatcgcggag
     5941 gctccgcagg gctcgtgccc agaggcggct gagggtgtca tcgccgggcg gccagtccgt
     6001 tgtaacagct gggaggcgat catctgggat tacttttact atgccgatga agtaccacca
     6061 gtggactggc ctacaaag
//
