LOCUS       NZ_CP061619             3858 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS51 plasmid pAS51-6, complete
            sequence.
ACCESSION   NZ_CP061619
VERSION     NZ_CP061619.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092230
            Assembly: GCF_014720855.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:39:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,434
            Genes (coding)                    :: 4,286
            CDSs (with protein)               :: 4,286
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 87 of 148
            Pseudo Genes (incomplete)         :: 60 of 148
            Pseudo Genes (internal stop)      :: 25 of 148
            Pseudo Genes (multiple problems)  :: 20 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061619.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS51"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS51-6"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(3489..3858,1..1532))
                     /locus_tag="IC783_RS22525"
                     /old_locus_tag="IC783_22515"
     CDS             complement(join(3489..3858,1..1532))
                     /locus_tag="IC783_RS22525"
                     /old_locus_tag="IC783_22515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1529..1678)
                     /gene="mobC"
                     /locus_tag="IC783_RS22820"
     CDS             complement(1529..1678)
                     /gene="mobC"
                     /locus_tag="IC783_RS22820"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_223861193.1"
                     /translation="MLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLK
                     ELKK"
                     /name="mobC"
     gene            complement(1690..1974)
                     /locus_tag="IC783_RS22825"
     CDS             complement(1690..1974)
                     /locus_tag="IC783_RS22825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223861191.1"
                     /translation="MKRKDTWRPLCYADVFHDLVQGQAPETPKSKGKSMTAIKRNKVYK
                     VRFSDAEVQKIKDANILNIAKYLRDTSLAQIDATQGNAKKRIRKTTTQN"
                     /name="hypothetical protein"
     gene            complement(2239..3201)
                     /locus_tag="IC783_RS22535"
                     /old_locus_tag="IC783_22525"
     CDS             complement(2239..3201)
                     /locus_tag="IC783_RS22535"
                     /old_locus_tag="IC783_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     Typing          complement(4..1532)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctttctcgtt caactggttg cagtgcttca ctagtttgct gttgtagttc tctaattcgt
       61 ctatggttct gttctgtgcc tgaatgagct gcaaggcttg ctcttgctgc ttctgttgct
      121 ctttgagctg ctgctgttgc tgctcgataa tctgcaataa ctgtgcttcg agattcattt
      181 aattggtttc ctgcactatt cgcatgttgt tgctgtactg ctgcgctttc gggtcgatct
      241 ggacgcacca gttccatttt ttctctgtcc cacacttctg aatattcagg ctcgcccctt
      301 ggctttttaa ccactggact ttgttctcca tctctgtgat gtcgctgtat ttgctcttgc
      361 tgacgtaccc cagacaaata ctgctgataa gcgttataca tgccagtgaa atcaaggcta
      421 tattcgatgt aataagggtt ttctttaatt tggctgcttg ggctgtttct tggctcaagc
      481 tgttcaactg ctgcaagttc gcttgtagct gtttgatctg ctgttgttgt tcttgcaata
      541 gggctgtcat gccttgttgc tgctgcttct tggtttgagc tgtagtctgg tcgatctgct
      601 gcaatatctc gttcgttgct tcgctctgtt tctgctgtat atacgttttg gtgctgtcta
      661 tactgctgct caagctctgt gcttcgcttt gcactgtatt ccgcatatct ttgaatatct
      721 gactgatttc gctgtttagc ttctcgtcta tatcgttctg atgctgcgat agtaagttgt
      781 tggctcgttt ctgtaagtct aaaatcttgc tcatagattg cacctgtaag ccttatcggg
      841 cgggcttttt cgtcatcatt cggattttta atggataggc ttttcttggt ttctctggtg
      901 acttcaagac caagatccaa tagccatttt ttcatgctct gccgatctgt aattagtcca
      961 ccttcaacgg ctctcgttgc ttcaatatca agcgtttcct tgatttcatt aacagtttta
     1021 ggcaagtttt ttgccgttgt aacggcttgg cgatagaggg ggtcatctgc atcatgcagt
     1081 tgatattcat agtttgtaat cttcttaaac agatcaatac gaggacgatc agctttatca
     1141 taataaggct gtaaacgttg ccctgtaagg atttcaacat tagggattag gaagttaagc
     1201 tctaatcttc gctcccctgt ttcttcgttt aatttgtctt gatgctcaat ccacaagaca
     1261 cggtactggc tctgatccat gccagggaat aaggcttgct caaaatcctg catgattttt
     1321 tgtttatcct ggtcagataa atcatgctca taaaaactta ggcagccgga tgtatatttt
     1381 tttgcataag gtgacgtatc aattaatcct gatacttcgg aaacatcgcc ctgtaggact
     1441 tttgcataat ctcgatcacg gtctttacca agtaaataat ccagtggacc tttacttaaa
     1501 ccgttcccat atctaaagaa ttgaacaatc attttttcaa ttcctttaag gtctgatcaa
     1561 tccctattaa caggtgcaaa agccttgcag catcaagggg acgactgctt gcaatatcag
     1621 tatttattgc ctttgcaatc tgattgatgt tgcccccaat tcgggatagc tcaagcaaaa
     1681 agtcacggtc taattttgtg tagtggtttt cctgattctt tttttggcgt tgccttgggt
     1741 tgcatcaatt tgggcaagcg aagtatccct tagatatttg gcaatattta ggatattggc
     1801 atctttgatt ttctgtacct ctgcatcaga aaatcggact ttgtaaacct tattacgttt
     1861 gatcgctgtc atgcttttgc ctttgctttt gggggtttcg ggggcttgcc cctgaacaag
     1921 gtcatggaac acatctgcgt agcagagggg tctccatgta tccttgcgtt tcattacgat
     1981 agcagacccc tagacgtaat tcaattaaga ttaagcaggt catagcaaga ctatggacaa
     2041 tcgcaagcga ccgtaaaacc tgatctgtgc ctaaacacta tgcacaactc cgcataacga
     2101 taaagccgtt atacgttgag ttgcacacag gtttagatca cagggtttta ctttgcccac
     2161 agccgttgct cattcccttt aataataatt tttaaaaatg aaatgggaaa aaagcaccct
     2221 agggggctga tatctgattt aggcttgcac tttccgcttt tttatccacg ttctgattgt
     2281 tctatccgaa acattcaatt gtagggctat atctttctgc tttatgccct gtatatgcag
     2341 ttttaaggct tcctgacgca tatcactgta ttgctgtgac ctagccttac cgccatgact
     2401 ggaatcactg cttagaccgc ctttacgccc tttgacagtc tgacgctcga taaatttggc
     2461 atgtgcttct gtgtgattgc gccataccca acgagcaata gacttagcgg ttgcctttac
     2521 ctctgaatat ggcaatggct ctaaaaacgc accatttgcg tttatagcga cttttagcac
     2581 ttccgcatac caactatcag gcgaccccga aataaacgct ctaatcgctt catacgccca
     2641 aaaacgaacg gtatcgaaca tcatacagtt acgcccaagc cctgaaactt cttttaagtc
     2701 ggtttttagt tcctgaagct ctacaaaatc ggctaaataa tttaggtcgt atggttgaac
     2761 gccaaaggta tgattttccc atgttccgtg acatggattt ttagtgatta accccgtata
     2821 gccacgatct gcacctaacg ccaagctata cgcctgctca atcttggcaa ggtactgtat
     2881 gacattctgc tttgctcgat gcgtggtaca cactggagct aaaagcatat agccaatatg
     2941 tgcatgtcca ttttggcgat ttttggctat ccatgttggg gtcggtaaat ttgcgtcaga
     3001 ccacgcaaaa taagcgtctt ggcggtctat atcgaacacc aacgagctaa caagacaagg
     3061 gggattatgc tgaatgtatg gcttctggat tgcggtacgt tttgggcgaa taatcacccc
     3121 acgccctaaa tcatccgagc aatacggttt ttttgggagt ttttcgtaaa aatcgtctga
     3181 aaattgcgtt ttattgagca tgaaacacgt ccaagtgttg cattggctca aaacatcagt
     3241 aaaatacaag tatcgaattt gagttttacg gatgctttaa gcgtttgttt aacaaagaga
     3301 aaaaagcggg tcgccaaacc tgcttttttt ttcgcctgat gaaaatatta cacctttttg
     3361 aattgctgtc aaatgactga ttttcaaaac aaaatattgc gacaaaatgt cgcaaatatg
     3421 tgacctttga agatgctttt ttttcgagga cagcattgca agggcaatgc tccgcagaaa
     3481 aaaaagtatc aaaatctagg tgaataactc ctgtcatttt tacgttgttg ctccagttgc
     3541 aactctctac gtctgtccag ttcatctaaa gccttgaaga tatttgtttg atcaaaccct
     3601 cgaatttttc gtgatactgt tgcactgagt ctagtttcct ttgaagctgt tcgttcaatg
     3661 gtgctgtctg cttctcgatt tctgtataga tcatcgttgg caatagttcg ttgatctcgt
     3721 tctttagatt taacccagtc actaattgct ctaaatggct ctgtaatcgc tgttttaagc
     3781 tgctctccaa gccttgcaat aaatcctctg atagcttcgg attctgtagt ttcttgatga
     3841 tattgatcag ctcgtttt
//
