LOCUS       NZ_CP061618             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS51 plasmid pAS51-5, complete
            sequence.
ACCESSION   NZ_CP061618
VERSION     NZ_CP061618.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092230
            Assembly: GCF_014720855.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:39:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,434
            Genes (coding)                    :: 4,286
            CDSs (with protein)               :: 4,286
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 87 of 148
            Pseudo Genes (incomplete)         :: 60 of 148
            Pseudo Genes (internal stop)      :: 25 of 148
            Pseudo Genes (multiple problems)  :: 20 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061618.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS51"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS51-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5607..6078,1..14)
                     /locus_tag="IC783_RS22495"
                     /old_locus_tag="IC783_22485"
     CDS             join(5607..6078,1..14)
                     /locus_tag="IC783_RS22495"
                     /old_locus_tag="IC783_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            147..815
                     /locus_tag="IC783_RS22500"
                     /old_locus_tag="IC783_22490"
     CDS             147..815
                     /locus_tag="IC783_RS22500"
                     /old_locus_tag="IC783_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(974..1507)
                     /gene="aadB"
                     /locus_tag="IC783_RS22505"
                     /old_locus_tag="IC783_22495"
     CDS             complement(974..1507)
                     /gene="aadB"
                     /locus_tag="IC783_RS22505"
                     /old_locus_tag="IC783_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(1598..2284)
                     /locus_tag="IC783_RS22510"
                     /old_locus_tag="IC783_22500"
     CDS             complement(1598..2284)
                     /locus_tag="IC783_RS22510"
                     /old_locus_tag="IC783_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            3287..3694
                     /locus_tag="IC783_RS22515"
                     /old_locus_tag="IC783_22505"
     CDS             3287..3694
                     /locus_tag="IC783_RS22515"
                     /old_locus_tag="IC783_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3684..5498
                     /locus_tag="IC783_RS22520"
                     /old_locus_tag="IC783_22510"
     CDS             3684..5498
                     /locus_tag="IC783_RS22520"
                     /old_locus_tag="IC783_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             complement(978..1507)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(975..1519)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          3685..5495
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caatcaatat gtaagctttt aattgaccaa aaaaagaaaa aagcctttca cgttaatgaa
       61 aggctttttt gaatcttagc gcttgggagg gaagatctta gatccgcagt ctctcaaact
      121 tacaactata tattagggta tatacggtgg aattgtcaaa tgatgcagtg cttaagatag
      181 agcaggcttt atctatacca cgcttatcta agtatgaaaa tttctataaa gataaaggtg
      241 aaccatatga aaaatctgat gtattgatgc tttatgaacg taatttaata atttccaata
      301 agttttttta cttattaaat tattttgaag ttgttctgag aaatgctgtt gttcaggcaa
      361 tagaaatatc ttttaggtgt aatgaaacaa atagttggca tgaaaatgaa gcttttattc
      421 gaagccttag ccgtagaggg aggtatagcc ctaaaagtat gtttgatagt gctaaagaga
      481 agttcccaga ttcgcctagt aaaatgatcc ctgagcttaa atttgtattc tggcaaaaga
      541 tgcttatggc taattatgaa gaacgtattt ggcagggatg ttttaattct atttttccta
      601 atgcaacagt tgaaaacagg caaatttttt acgattggac agatcaatta cgtgaattaa
      661 gaaacaggat tgctcatcat gaaccgatta tttttaaccg taatctagaa aatgatttag
      721 aggttttaac aaaatttatt tactgtcgtt gtattaatac atacgatttt atcgagcaat
      781 ctgcattaga tctaaaagaa tatttagatt tttaagataa gaatttcacc ccaaacaatc
      841 gaaaggtcag actattttgc aaatgttgaa ttaacaaggt tagtagtaaa gcagtaaatc
      901 cgataacggt agcagtgttt cctaacacct gagttaagcc gcgccgcaaa gcggcgtcgg
      961 cttggacgaa ttgttaggcc gcatatcgcg acctgaaagc ggcacgcaag acctcaacct
     1021 tttccgcccc gagtgaggtg catgcgaacc tgtaggactc tatgtgcttt gtaggccagt
     1081 ccactggtgg tacttcatcg gcatagtaaa agtaatccca gatgatcgcc tcccagctgt
     1141 tacaacggac tggccgcccg gcgatgacac cctcagccgc ctctgggcac gagccctgcg
     1201 gagcctccgc gatttcatac gcttcgtctg cccaccaagc aggttcgcag tcaagtaact
     1261 catccccgat ctccgctaag aatccatagt ccaactcctc cgtgacgcgc ccgccgagca
     1321 tttcaactat tgcctcgagc tcgccgcgcc tctcgccggg aaaagtcaga tcaatatcat
     1381 cgtgcttgcg tgttacacgc cctagccgtg catcgatcgc ccagccccca ccgatccaga
     1441 gcggcagatt tcgctcatct gccgcagcta gaatttggtg tatcaatgcg acctgcgttg
     1501 tgtccatgcg gcctaacata gtaagccctc ccagaatgtc agtaattaga ctattaattg
     1561 aattgttaca tccaatagtg agctcccagt gattgtctta actatccaac ctttcgattg
     1621 gatggggcag agcaccatta ttctgtttga atgcggtgtt agtgtcaact aggttcttta
     1681 tttcttcaag gagtttgaaa gtgctaagta tttttacttt actttgttcc tcaatataaa
     1741 tttttttaag accatttagc agcttgtatt tggatatttt ggataggaga atgaataagg
     1801 ttgcggtatt gattcttccc ccttttagat gaagtaaacg gtttttttgc agtattggaa
     1861 tatttctata ttctgttagt tgatctttta agtcatcagg aattttcatt tcgtaaaaat
     1921 tatatttttt agaacagtct aatatcgagt gatttaggct ttctgtattt gtattaataa
     1981 aaatgcattt ttcaaatgtg caatttttaa agcgacaatc tctaaaaata gcagcataaa
     2041 atacgcactt tttgaagtga acattcgtaa acctattctt atttaagatt actcctgaaa
     2101 aatcagataa aacaattttt atatttttga atgaacatga tcgtaggcgg gatcctctca
     2161 aattaacatt aaaaaatgtt ttattagaga aacgtacttt agaagaaata gatttggcta
     2221 ggaggctatt tgaatactta cgtttttcga tgtgcttgtt atgaaatcta ggaagttttg
     2281 acattaatag tccaaagaat aggagtttca gcttaattct taggtgtaat caaaaaaaat
     2341 aatggacagg tctcttgcga aatctgtcca ttagttttaa attcgatggg tacagttatt
     2401 tgatgcttgc aacatcaatt aacgaaagat cggcagagct tgcaactcat actgatattc
     2461 accttaaagg ctagtaccac tatctatttt aaagaattag aacagtttga caagcctcaa
     2521 tttgaagtcc ttcgttaatt cagcagttta aatcatcaaa taagcaccta acggtcgagc
     2581 tttatctact tctgtgcgcc ttttttaagg aagatatgcg ttgcatgtgg agggaataag
     2641 tgtcactggt gcatcgtagc gatagtcgca aggctaaacc gcttacaggg gatctcacat
     2701 aggcggagcg ttggaagtag ttgtataggt tggttaagtt gcatgttgga gtaatcctcg
     2761 cagagatacc accatacttc gacagggagc gcgtgggaga aggtgcaaaa cctttagtcc
     2821 ttgtctgcat ggtcagcaaa accatgtgcg gatacgaccg atgcgtggct ccgtcagccc
     2881 aattacaggt taatcggcaa tacttaagag gatatttgat ttttttaaat caaagcctct
     2941 tagggaaagt attgccgaaa ctctctcaac gttcaccatc agcccgagca aagcgaggga
     3001 tgtggggacg gtgagaaaga gttgagcgca tttgttttta caagattcat gccaatacct
     3061 actagagttt atataccgtg tcatttgatt agtttttatt gaatttcact cgtccatttt
     3121 taaataagca aggatacaca gctaccccta tttaacttcg ttaaacagtg atagtctgtg
     3181 accttgatca ggggcttcac ccccgatacc cccgaaaagc tttaaacaga aaaatcaaag
     3241 attttccaat ttaaagctta caaaagccaa agcagggtag attgaaatgt caggacagga
     3301 acaagaaaac aaacagagca aagcagttcg agaacgaacc tttaaagtca gactttcaga
     3361 atcagaatat caattgctcg attccaataa tccttatggt tcgattgcaa agttgcttag
     3421 acaagcagca gtagagaaag cgactaaaat tcataattcc aaagcagaag atgaccaaaa
     3481 aattaaagca acaaaggttg ttgaatcttc gtatagcaag acagagcgag cattaatttt
     3541 agagcttggt cggattggca acaatgtgaa tcagattgct aaagcagtga ataccgatat
     3601 tgcaggggta ggtacttttg acaaagtgaa gttacttcat ttgttgattt ccatagatca
     3661 gcagcttcgg gagttacgtc cagatgatcg ttgatttctt tagacatggt tcaggtttat
     3721 caaaaggctg tctcgattat ttgttaggtg aagatcgaga gcgtgaacat gctcaagtgc
     3781 tcaatggtga tgtagaactg acagcacaac tcattgatag cagtcctttt gccaaaaaat
     3841 ataccagtgg ttgtttgtcc ttttatgagc atgatttatc tgaccaggat aaacagaaaa
     3901 tcatgcaaaa ttttgaggaa tgtttattcc cgggactaga taaagaccaa tatcaaattt
     3961 tatgggtaca gcaccaagac aaaattaatc aggacacagg tgagacaagg cttgagctta
     4021 attttgtgat tcctaatgtt gagctttcaa caggcaaacg cctacagcca ttctatgcgc
     4081 cagtcgattt agatcgagtc gatctattta agcagatcac taataccgaa cattcactgt
     4141 atgaccccga tgatcctgag catcgacagc tatttttaaa taaaaagaac ctacccaaag
     4201 atattaagga ttttaaagcg cagttacatc aacgtgttta ccgagcggtt gccaatggtg
     4261 atgttgccga tcggcaagaa ttggtgcaat ggcttgaatc gaatcaaatc aatgtcactc
     4321 gacaagtaaa aaactccata tccattgaaa acccatatga gggcgcaaaa cgtcccatcc
     4381 gtttagaggg cgagatttat gagcaaggct ttagagctac tggcgaatat agacaagaag
     4441 tacaacaacg aattgaagaa tacagaggaa caacttctga gcggtatcga acaaacgtca
     4501 cagactatca gagacagctt gaacataaga gccaatatca ctgcgaccgc tatccaacag
     4561 tcggacgaga aaatagccca gagcattcaa aacaacgccc agacggtcga gaagcagtta
     4621 agcctgtatc aaaccttgct gcaatcgaaa ttgagccatt taacgcaatt aaacgagcaa
     4681 atacagagcc aagaacaaca agcccagaat caagcggtac agaaaaaact taccactttg
     4741 aatatgggac tgatttcagt agcagttatt ttgcttatag caattttctc gcttggtctc
     4801 gtcaccaaaa acaagtacag cgagatgcag acgctaaata ccgtgatcaa cgagaagcaa
     4861 atgagggtcg accatttgaa ccgatcggga ggcagtttga cgatcagcac atgcaagcga
     4921 ccagacagca aagcacagcg cctatgtatt cagatcaaca agaacgcagg cgaatggcag
     4981 aacggctaca tgattccaat ggggtattaa acgatgaccg aattagaaac acaattattg
     5041 agaatcatcg aagaacaaca gcaacaatta atgcaacaac aagtgcaatt gcagaagcaa
     5101 cagcaagctt tagacacaat gcaaaccaaa attatccaag ccttgtcagc acaatcaaag
     5161 acaatcaaga gcgatcagca aagcttacag caagcacaga aatcattagc gacaatgcag
     5221 aagcaatttc acgagctaga acgaagtaca gcaagctata ccgagcagat caatggcaaa
     5281 gtccgagaga tgagccttat cacagccaac aacgaagaaa ttctggagag aatctcaacc
     5341 acacagcatt ctgtctcgtc agaagtagcc aaagcgcagc agaatttgct cgaaacatcc
     5401 gagatattga aaaaaatctc gtccagatga atgagcgtaa acaagagata gaaaaacctc
     5461 gacctaagca agatcgaggt atggattttg ggatgtaatt attttctagt atgttggtgt
     5521 ttatttctta gtattagctt gtactaatgt ttaaatttag ctatattgaa tgtagtatag
     5581 gcttgtttat atattttggt ggtgttatga ataataactg tgtagtagta ctaactgctc
     5641 gtggaaagac tagaatttta aaagaaggtg gtagtcagtc ttggagacta aatgctcata
     5701 atgcaggaaa gtgcgattat ctagtttgtg tgcaaaatag gaaacaatct tggggagaac
     5761 cagaggctga tcatcacact gcatttttag ttggcaaaat atctagtatc agccaatctc
     5821 aagaagaaga tgcagatggt agatggataa taaatatctc cgaatatgct gatatatcta
     5881 ttcccaatat gtgggatggt aatcggaatc cagtttctta tagttcatta gaagagatga
     5941 atatcgattt gaatgagctg aaatttacca aattaaatga agataattct tctaaaacac
     6001 tggataaaat atctattcct gaagctaaga gactattagc taattatttt gatgtccctc
     6061 aagaaaatat ttcaatta
//
