LOCUS       NZ_CP061608             3857 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS53 plasmid pAS53-5, complete
            sequence.
ACCESSION   NZ_CP061608
VERSION     NZ_CP061608.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092232
            Assembly: GCF_014720495.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:22:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 4,229
            CDSs (with protein)               :: 4,229
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 76 of 143
            Pseudo Genes (incomplete)         :: 65 of 143
            Pseudo Genes (internal stop)      :: 23 of 143
            Pseudo Genes (multiple problems)  :: 17 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061608.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS53"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS53-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(3812..3857,1..917)
                     /locus_tag="IC785_RS22195"
                     /old_locus_tag="IC785_22185"
     CDS             join(3812..3857,1..917)
                     /locus_tag="IC785_RS22195"
                     /old_locus_tag="IC785_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     gene            1182..1466
                     /locus_tag="IC785_RS22490"
     CDS             1182..1466
                     /locus_tag="IC785_RS22490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223861191.1"
                     /translation="MKRKDTWRPLCYADVFHDLVQGQAPETPKSKGKSMTAIKRNKVYK
                     VRFSDAEVQKIKDANILNIAKYLRDTSLAQIDATQGNAKKRIRKTTTQN"
                     /name="hypothetical protein"
     gene            1478..1627
                     /gene="mobC"
                     /locus_tag="IC785_RS22495"
     CDS             1478..1627
                     /gene="mobC"
                     /locus_tag="IC785_RS22495"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_223861193.1"
                     /translation="MLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLK
                     ELKK"
                     /name="mobC"
     gene            1624..3525
                     /locus_tag="IC785_RS22205"
                     /old_locus_tag="IC785_22195"
     CDS             1624..3525
                     /locus_tag="IC785_RS22205"
                     /old_locus_tag="IC785_22195"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          1625..3519
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.575"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caaaaaaacc gtattgctcg gatgatttag ggcgtggggt gattattcgc ccaaaacgta
       61 ccgcaatcca gaagccatac attcagcata atcccccttg tcttgttagc tcgttggtgt
      121 tcgatataga ccgccaagac gcttattttg cgtggtctga cgcaaattta ccgaccccaa
      181 catggatagc caaaaatcgc caaaatggac atgcacatat tggctatatg cttttagctc
      241 cagtgtgtac cacgcatcga gcaaagcaga atgtcataca gtaccttgcc aagattgagc
      301 aggcgtatag cttggcgtta ggtgcagatc gtggctatac ggggttaatc actaaaaatc
      361 catgtcacgg aacatgggaa aatcatacct ttggcgttca accatacgac ctaaattatt
      421 tagccgattt tgtagagctt caggaactaa aaaccgactt aaaagaagtt tcagggcttg
      481 ggcgtaactg tatgatgttc gataccgttc gtttttgggc gtatgaagcg attagagcgt
      541 ttatttcggg gtcgcctgat agttggtatg cggaagtgct aaaagtcgct ataaacgcaa
      601 atggtgcgtt tttagagcca ttgccatatt cagaggtaaa ggcaaccgct aagtctattg
      661 ctcgttgggt atggcgcaat cacacagaag cacatgccaa atttatcgag cgtcagactg
      721 tcaaagggcg taaaggcggt ctaagcagtg attccagtca tggcggtaag gctaggtcac
      781 agcaatacag tgatatgcgt caggaagcct taaaactgca tatacagggc ataaagcaga
      841 aagatatagc cctacaattg aatgtttcgg atagaacaat cagaacgtgg ataaaaaagc
      901 ggaaagtgca agcctaaatc agatatcagc cccctagggt gcttttttcc catttcattt
      961 ttaaaaatta ttattaaagg gaatgagcaa cggctgtggg caaagtaaaa ccctgtgatc
     1021 taaacctgtg tgcaactcaa cgtataacgg ctttatcgtt atgcggagtt gtgcatagtg
     1081 tttaggcaca gatcaggttt tacggtcgct tgcgattgtc catagtcttg ctatgacctg
     1141 cttaatctta attgaattac gtctaggggt ctgctatcgt aatgaaacgc aaggatacat
     1201 ggagacccct ctgctacgca gatgtgttcc atgaccttgt tcaggggcaa gcccccgaaa
     1261 cccccaaaag caaaggcaaa agcatgacag cgatcaaacg taataaggtt tacaaagtcc
     1321 gattttctga tgcagaggta cagaaaatca aagatgccaa tatcctaaat attgccaaat
     1381 atctaaggga tacttcgctt gcccaaattg atgcaaccca aggcaacgcc aaaaaaagaa
     1441 tcaggaaaac cactacacaa aattagaccg tgactttttg cttgagctat cccgaattgg
     1501 gggcaacatc aatcagattg caaaggcaat aaatactgat attgcaagca gtcgtcccct
     1561 tgatgctgca aggcttttgc acctgttaat agggattgat cagaccttaa aggaattgaa
     1621 aaaatgattg ttcaattctt tagatatggg aacggtttaa gtaaaggtcc actggattat
     1681 ttacttggta aagaccgtga tcgagattat gcaaaagtcc tacagggcga tgtttccgaa
     1741 gtatcaggat taattgatac gtcaccttat gcaaaaaaat atacatccgg ctgcctaagt
     1801 ttttatgagc atgatttatc tgaccaggat aaacaaaaaa tcatgcagga ttttgagcaa
     1861 gccttattcc ctggcatgga tcagagccag taccgtgtct tgtggattga gcatcaagac
     1921 aaattaaacg aagaaacagg ggagcgaaga ttagagctta acttcctaat ccctaatgtt
     1981 gaaatcctta cagggcaacg tttacagcct tattatgata aagctgatcg tcctcgtatt
     2041 gatctgttta agaagattac aaactatgaa tatcaactgc atgatgcaga tgaccccctc
     2101 tatcgccaag ccgttacaac ggcaaaaaac ttgcctaaaa ctgttaatga aatcaaggaa
     2161 acgcttgata ttgaagcaac gagagccgtt gaaggtggac taattacaga tcggcagagc
     2221 atgaaaaaat ggctattgga tcttggtctt gaagtcacca gagaaaccaa gaaaagccta
     2281 tccattaaaa atccgaatga tgacgaaaaa gcccgcccga taaggcttac aggtgcaatc
     2341 tatgagcaag attttagact tacagaaacg agccaacaac ttactatcgc agcatcagaa
     2401 cgatatagac gagaagctaa acagcgaaat cagtcagata ttcaaagata tgcggaatac
     2461 agtgcaaagc gaagcacaga gcttgagcag cagtatagac agcaccaaaa cgtatataca
     2521 gcagaaacag agcgaagcaa cgaacgagat attgcagcag atcgaccaga ctacagctca
     2581 aaccaagaag cagcagcaac aaggcatgac agccctattg caagaacaac aacagcagat
     2641 caaacagcta caagcgaact tgcagcagtt gaacagcttg agccaagaaa cagcccaagc
     2701 agccaaatta aagaaaaccc ttattacatc gaatatagcc ttgatttcac tggcatgtat
     2761 aacgcttatc agcagtattt gtctggggta cgtcagcaag agcaaataca gcgacatcac
     2821 agagatggag aacaaagtcc agtggttaaa aagccaaggg gcgagcctga atattcagaa
     2881 gtgtgggaca gagaaaaaat ggaactggtg cgtccagatc gacccgaaag cgcagcagta
     2941 cagcaacaac atgcgaatag tgcaggaaac caattaaatg aatctcgaag cacagttatt
     3001 gcagattatc gagcagcaac agcagcagct caaagagcaa cagaagcagc aagagcaagc
     3061 cttgcagctc attcaggcac agaacagaac catagacgaa ttagagaact acaacagcaa
     3121 actagtgaag cactgcaacc agttgaacga gaaagaaaac gagctgatca atatcatcaa
     3181 gaaactacag aatccgaagc tatcagagga tttattgcaa ggcttggaga gcagcttaaa
     3241 acagcgatta cagagccatt tagagcaatt agtgactggg ttaaatctaa agaacgagat
     3301 caacgaacta ttgccaacga tgatctatac agaaatcgag aagcagacag caccattgaa
     3361 cgaacagctt caaaggaaac tagactcagt gcaacagtat cacgaaaaat tcgagggttt
     3421 gatcaaacaa atatcttcaa ggctttagat gaactggaca gacgtagaga gttgcaactg
     3481 gagcaacaac gtaaaaatga caggagttat tcacctagat tttgatactt ttttttctgc
     3541 ggagcattgc ccttgcaatg ctgtcctcga aaaaaaagca tcttcaaagg tcacatattt
     3601 gcgacatttt gtcgcaatat tttgttttga aaatcagtca tttgacagca attcaaaaag
     3661 gtgtaatatt ttcatcaggc gaaaaaaaag caggtttggc gacccgcttt tttctctttg
     3721 ttaaacaaac gcttaaagca tccgtaaaac tcaaattcga tacttgtatt ttactgatgt
     3781 tttgagccaa tgcaacactt ggacgtgttt catgctcaat aaaacgcaat tttcagacga
     3841 tttttacgaa aaactcc
//
