LOCUS       NZ_CP061607             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS53 plasmid pAS53-4, complete
            sequence.
ACCESSION   NZ_CP061607
VERSION     NZ_CP061607.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092232
            Assembly: GCF_014720495.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:22:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 4,229
            CDSs (with protein)               :: 4,229
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 76 of 143
            Pseudo Genes (incomplete)         :: 65 of 143
            Pseudo Genes (internal stop)      :: 23 of 143
            Pseudo Genes (multiple problems)  :: 17 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061607.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS53"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS53-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(5817..6078,1..224))
                     /locus_tag="IC785_RS22165"
                     /old_locus_tag="IC785_22155"
     CDS             complement(join(5817..6078,1..224))
                     /locus_tag="IC785_RS22165"
                     /old_locus_tag="IC785_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(333..2147)
                     /locus_tag="IC785_RS22170"
                     /old_locus_tag="IC785_22160"
     CDS             complement(333..2147)
                     /locus_tag="IC785_RS22170"
                     /old_locus_tag="IC785_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2137..2544)
                     /locus_tag="IC785_RS22175"
                     /old_locus_tag="IC785_22165"
     CDS             complement(2137..2544)
                     /locus_tag="IC785_RS22175"
                     /old_locus_tag="IC785_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3547..4233
                     /locus_tag="IC785_RS22180"
                     /old_locus_tag="IC785_22170"
     CDS             3547..4233
                     /locus_tag="IC785_RS22180"
                     /old_locus_tag="IC785_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            4324..4857
                     /gene="aadB"
                     /locus_tag="IC785_RS22185"
                     /old_locus_tag="IC785_22175"
     CDS             4324..4857
                     /gene="aadB"
                     /locus_tag="IC785_RS22185"
                     /old_locus_tag="IC785_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(5016..5684)
                     /locus_tag="IC785_RS22190"
                     /old_locus_tag="IC785_22180"
     CDS             complement(5016..5684)
                     /locus_tag="IC785_RS22190"
                     /old_locus_tag="IC785_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     ARG             4325..4854
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             4313..4857
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(337..2147)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttcttctt gagattggct gatactagat attttgccaa ctaaaaatgc agtgtgatga
       61 tcagcctctg gttctcccca agattgtttc ctattttgca cacaaactag ataatcgcac
      121 tttcctgcat tatgagcatt tagtctccaa gactgactac caccttcttt taaaattcta
      181 gtctttccac gagcagttag tactactaca cagttattat tcataacacc accaaaatat
      241 ataaacaagc ctatactaca ttcaatatag ctaaatttaa acattagtac aagctaatac
      301 taagaaataa acaccaacat actagaaaat aattacatcc caaaatccat acctcgatct
      361 tgcttaggtc gaggtttttc tatctcttgt ttacgctcat tcatctggac gagatttttt
      421 tcaatatctc ggatgtttcg agcaaattct gctgcgcttt ggctacttct gacgagacag
      481 aatgctgtgt ggttgagatt ctctccagaa tttcttcgtt gttggctgtg ataaggctca
      541 tctctcggac tttgccattg atctgctcgg tatagcttgc tgtacttcgt tctagctcgt
      601 gaaattgctt ctgcattgtc gctaatgatt tctgtgcttg ctgtaagctt tgctgatcgc
      661 tcttgattgt ctttgattgt gctgacaagg cttggataat tttggtttgc attgtgtcta
      721 aagcttgctg ttgcttctgc aattgcactt gttgttgcat taattgttgc tgttgttctt
      781 cgatgattct caataattgt gtttctaatt cggtcatcgt ttaatacccc attggaatca
      841 tgtagccgtt ctgccattcg cctgcgttct tgttgatctg aatacatagg cgctgtgctt
      901 tgctgtctgg tcgcttgcat gtgctgatcg tcaaactgcc tcccgatcgg ttcaaatggt
      961 cgaccctcat ttgcttctcg ttgatcacgg tatttagcgt ctgcatctcg ctgtacttgt
     1021 ttttggtgac gagaccaagc gagaaaattg ctataagcaa aataactgct actgaaatca
     1081 gtcccatatt caaagtggta agttttttct gtaccgcttg attctgggct tgttgttctt
     1141 ggctctgtat ttgctcgttt aattgcgtta aatggctcaa tttcgattgc agcaaggttt
     1201 gatacaggct taactgcttc tcgaccgtct gggcgttgtt ttgaatgctc tgggctattt
     1261 tctcgtccga ctgttggata gcggtcgcag tgatattggc tcttatgttc aagctgtctc
     1321 tgatagtctg tgacgtttgt tcgataccgc tcagaagttg ttcctctgta ttcttcaatt
     1381 cgttgttgta cttcttgtct atattcgcca gtagctctaa agccttgctc ataaatctcg
     1441 ccctctaaac ggatgggacg ttttgcgccc tcatatgggt tttcaatgga tatggagttt
     1501 tttacttgtc gagtgacatt gatttgattc gattcaagcc attgcaccaa ttcttgccga
     1561 tcggcaacat caccattggc aaccgctcgg taaacacgtt gatgtaactg cgctttaaaa
     1621 tccttaatat ctttgggtag gttcttttta tttaaaaata gctgtcgatg ctcaggatca
     1681 tcggggtcat acagtgaatg ttcggtatta gtgatctgct taaatagatc gactcgatct
     1741 aaatcgactg gcgcatagaa tggctgtagg cgtttgcctg ttgaaagctc aacattagga
     1801 atcacaaaat taagctcaag ccttgtctca cctgtgtcct gattaatttt gtcttggtgc
     1861 tgtacccata aaatttgata ttggtcttta tctagtcccg ggaataaaca ttcctcaaaa
     1921 ttttgcatga ttttctgttt atcctggtca gataaatcat gctcataaaa ggacaaacaa
     1981 ccactggtat attttttggc aaaaggactg ctatcaatga gttgtgctgt cagttctaca
     2041 tcaccattga gcacttgagc atgttcacgc tctcgatctt cacctaacaa ataatcgaga
     2101 cagccttttg ataaacctga accatgtcta aagaaatcaa cgatcatctg gacgtaactc
     2161 ccgaagctgc tgatctatgg aaatcaacaa atgaagtaac ttcactttgt caaaagtacc
     2221 tacccctgca atatcggtat tcactgcttt agcaatctga ttcacattgt tgccaatccg
     2281 accaagctct aaaattaatg ctcgctctgt cttgctatac gaagattcaa caacctttgt
     2341 tgctttaatt ttttggtcat cttctgcttt ggaattatga attttagtcg ctttctctac
     2401 tgctgcttgt ctaagcaact ttgcaatcga accataagga ttattggaat cgagcaattg
     2461 atattctgat tctgaaagtc tgactttaaa ggttcgttct cgaactgctt tgctctgttt
     2521 gttttcttgt tcctgtcctg acatttcaat ctaccctgct ttggcttttg taagctttaa
     2581 attggaaaat ctttgatttt tctgtttaaa gcttttcggg ggtatcgggg gtgaagcccc
     2641 tgatcaaggt cacagactat cactgtttaa cgaagttaaa taggggtagc tgtgtatcct
     2701 tgcttattta aaaatggacg agtgaaattc aataaaaact aatcaaatga cacggtatat
     2761 aaactctagt aggtattggc atgaatcttg taaaaacaaa tgcgctcaac tctttctcac
     2821 cgtccccaca tccctcgctt tgctcgggct gatggtgaac gttgagagag tttcggcaat
     2881 actttcccta agaggctttg atttaaaaaa atcaaatatc ctcttaagta ttgccgatta
     2941 acctgtaatt gggctgacgg agccacgcat cggtcgtatc cgcacatggt tttgctgacc
     3001 atgcagacaa ggactaaagg ttttgcacct tctcccacgc gctccctgtc gaagtatggt
     3061 ggtatctctg cgaggattac tccaacatgc aacttaacca acctatacaa ctacttccaa
     3121 cgctccgcct atgtgagatc ccctgtaagc ggtttagcct tgcgactatc gctacgatgc
     3181 accagtgaca cttattccct ccacatgcaa cgcatatctt ccttaaaaaa ggcgcacaga
     3241 agtagataaa gctcgaccgt taggtgctta tttgatgatt taaactgctg aattaacgaa
     3301 ggacttcaaa ttgaggcttg tcaaactgtt ctaattcttt aaaatagata gtggtactag
     3361 cctttaaggt gaatatcagt atgagttgca agctctgccg atctttcgtt aattgatgtt
     3421 gcaagcatca aataactgta cccatcgaat ttaaaactaa tggacagatt tcgcaagaga
     3481 cctgtccatt attttttttg attacaccta agaattaagc tgaaactcct attctttgga
     3541 ctattaatgt caaaacttcc tagatttcat aacaagcaca tcgaaaaacg taagtattca
     3601 aatagcctcc tagccaaatc tatttcttct aaagtacgtt tctctaataa aacatttttt
     3661 aatgttaatt tgagaggatc ccgcctacga tcatgttcat tcaaaaatat aaaaattgtt
     3721 ttatctgatt tttcaggagt aatcttaaat aagaataggt ttacgaatgt tcacttcaaa
     3781 aagtgcgtat tttatgctgc tatttttaga gattgtcgct ttaaaaattg cacatttgaa
     3841 aaatgcattt ttattaatac aaatacagaa agcctaaatc actcgatatt agactgttct
     3901 aaaaaatata atttttacga aatgaaaatt cctgatgact taaaagatca actaacagaa
     3961 tatagaaata ttccaatact gcaaaaaaac cgtttacttc atctaaaagg gggaagaatc
     4021 aataccgcaa ccttattcat tctcctatcc aaaatatcca aatacaagct gctaaatggt
     4081 cttaaaaaaa tttatattga ggaacaaagt aaagtaaaaa tacttagcac tttcaaactc
     4141 cttgaagaaa taaagaacct agttgacact aacaccgcat tcaaacagaa taatggtgct
     4201 ctgccccatc caatcgaaag gttggatagt taagacaatc actgggagct cactattgga
     4261 tgtaacaatt caattaatag tctaattact gacattctgg gagggcttac tatgttaggc
     4321 cgcatggaca caacgcaggt cgcattgata caccaaattc tagctgcggc agatgagcga
     4381 aatctgccgc tctggatcgg tgggggctgg gcgatcgatg cacggctagg gcgtgtaaca
     4441 cgcaagcacg atgatattga tctgactttt cccggcgaga ggcgcggcga gctcgaggca
     4501 atagttgaaa tgctcggcgg gcgcgtcacg gaggagttgg actatggatt cttagcggag
     4561 atcggggatg agttacttga ctgcgaacct gcttggtggg cagacgaagc gtatgaaatc
     4621 gcggaggctc cgcagggctc gtgcccagag gcggctgagg gtgtcatcgc cgggcggcca
     4681 gtccgttgta acagctggga ggcgatcatc tgggattact tttactatgc cgatgaagta
     4741 ccaccagtgg actggcctac aaagcacata gagtcctaca ggttcgcatg cacctcactc
     4801 ggggcggaaa aggttgaggt cttgcgtgcc gctttcaggt cgcgatatgc ggcctaacaa
     4861 ttcgtccaag ccgacgccgc tttgcggcgc ggcttaactc aggtgttagg aaacactgct
     4921 accgttatcg gatttactgc tttactacta accttgttaa ttcaacattt gcaaaatagt
     4981 ctgacctttc gattgtttgg ggtgaaattc ttatcttaaa aatctaaata ttcttttaga
     5041 tctaatgcag attgctcgat aaaatcgtat gtattaatac aacgacagta aataaatttt
     5101 gttaaaacct ctaaatcatt ttctagatta cggttaaaaa taatcggttc atgatgagca
     5161 atcctgtttc ttaattcacg taattgatct gtccaatcgt aaaaaatttg cctgttttca
     5221 actgttgcat taggaaaaat agaattaaaa catccctgcc aaatacgttc ttcataatta
     5281 gccataagca tcttttgcca gaatacaaat ttaagctcag ggatcatttt actaggcgaa
     5341 tctgggaact tctctttagc actatcaaac atacttttag ggctatacct ccctctacgg
     5401 ctaaggcttc gaataaaagc ttcattttca tgccaactat ttgtttcatt acacctaaaa
     5461 gatatttcta ttgcctgaac aacagcattt ctcagaacaa cttcaaaata atttaataag
     5521 taaaaaaact tattggaaat tattaaatta cgttcataaa gcatcaatac atcagatttt
     5581 tcatatggtt cacctttatc tttatagaaa ttttcatact tagataagcg tggtatagat
     5641 aaagcctgct ctatcttaag cactgcatca tttgacaatt ccaccgtata taccctaata
     5701 tatagttgta agtttgagag actgcggatc taagatcttc cctcccaagc gctaagattc
     5761 aaaaaagcct ttcattaacg tgaaaggctt ttttcttttt ttggtcaatt aaaagcttac
     5821 atattgattg taattgaaat attttcttga gggacatcaa aataattagc taatagtctc
     5881 ttagcttcag gaatagatat tttatccagt gttttagaag aattatcttc atttaatttg
     5941 gtaaatttca gctcattcaa atcgatattc atctcttcta atgaactata agaaactgga
     6001 ttccgattac catcccacat attgggaata gatatatcag catattcgga gatatttatt
     6061 atccatctac catctgca
//
