LOCUS       NZ_CP061602             6077 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS58 plasmid pAS58-4, complete
            sequence.
ACCESSION   NZ_CP061602
VERSION     NZ_CP061602.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092234
            Assembly: GCF_014720295.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:26:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,308
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 445
            CDSs (without protein)            :: 445
            Pseudo Genes (ambiguous residues) :: 0 of 445
            Pseudo Genes (frameshifted)       :: 361 of 445
            Pseudo Genes (incomplete)         :: 83 of 445
            Pseudo Genes (internal stop)      :: 23 of 445
            Pseudo Genes (multiple problems)  :: 18 of 445
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061602.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS58"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS58-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5639..6077,1..1376)
                     /locus_tag="IC787_RS21715"
                     /old_locus_tag="IC787_21700"
     CDS             join(5639..6077,1..1376)
                     /locus_tag="IC787_RS21715"
                     /old_locus_tag="IC787_21700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1485..1970
                     /locus_tag="IC787_RS21720"
                     /old_locus_tag="IC787_21705"
     CDS             1485..1970
                     /locus_tag="IC787_RS21720"
                     /old_locus_tag="IC787_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            2103..2771
                     /locus_tag="IC787_RS21725"
                     /old_locus_tag="IC787_21710"
     CDS             2103..2771
                     /locus_tag="IC787_RS21725"
                     /old_locus_tag="IC787_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(2930..3463)
                     /gene="aadB"
                     /locus_tag="IC787_RS21730"
                     /old_locus_tag="IC787_21715"
     CDS             complement(2930..3463)
                     /gene="aadB"
                     /locus_tag="IC787_RS21730"
                     /old_locus_tag="IC787_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3554..4240)
                     /locus_tag="IC787_RS21735"
                     /old_locus_tag="IC787_21720"
     CDS             complement(3554..4240)
                     /locus_tag="IC787_RS21735"
                     /old_locus_tag="IC787_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5242..5649
                     /locus_tag="IC787_RS21740"
                     /old_locus_tag="IC787_21725"
     CDS             5242..5649
                     /locus_tag="IC787_RS21740"
                     /old_locus_tag="IC787_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(2934..3463)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(2931..3475)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          4..1373
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5640..6076
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="2.05e-93"
ORIGIN
        1 ataccgaaca ttcactgtat gaccccgatg atcctgagca tcgacagcta tttttaaata
       61 aaaagaacct acccaaagat attaaggatt ttaaagcgca gttacatcaa cgtgtttacc
      121 gagcggttgc caatggtgat gttgccgatc ggcaagaatt ggtgcaatgg cttgaatcga
      181 atcaaatcaa tgtcactcga caagtaaaaa actccatatc cattgaaaac ccatatgagg
      241 gcgcaaaacg tcccatccgt ttagagggcg agatttatga gcaaggcttt agagctactg
      301 gcgaatatag acaagaagta caacaacgaa ttgaagaata cagaggaaca acttctgagc
      361 ggtatcgaac aaacgtcaca gactatcaga gacagcttga acataagagc caatatcact
      421 gcgaccgcta tccaacagtc ggacgagaaa atagcccaga gcattcaaaa caacgcccag
      481 acggtcgaga agcagttaag cctgtatcaa accttgctgc aatcgaaatt gagccattta
      541 acgcaattaa acgagcaaat acagagccaa gaacaacaag cccagaatca agcggtacag
      601 aaaaaactta ccactttgaa tatgggactg atttcagtag cagttatttt gcttatagca
      661 attttctcgc ttggtctcgt caccaaaaac aagtacagcg agatgcagac gctaaatacc
      721 gtgatcaacg agaagcaaat gagggtcgac catttgaacc gatcgggagg cagtttgacg
      781 atcagcacat gcaagcgacc agacagcaaa gcacagcgcc tatgtattca gatcaacaag
      841 aacgcaggcg aatggcagaa cggctacatg attccaatgg ggtattaaac gatgaccgaa
      901 ttagaaacac aattattgag aatcatcgaa gaacaacagc aacaattaat gcaacaacaa
      961 gtgcaattgc agaagcaaca gcaagcttta gacacaatgc aaaccaaaat tatccaagcc
     1021 ttgtcagcac aatcaaagac aatcaagagc gatcagcaaa gcttacagca agcacagaaa
     1081 tcattagcga caatgcagaa gcaatttcac gagctagaac gaagtacagc aagctatacc
     1141 gagcagatca atggcaaagt ccgagagatg agccttatca cagccaacaa cgaagaaatt
     1201 ctggagagaa tctcaaccac acagcattct gtctcgtcag aagtagccaa agcgcagcag
     1261 aatttgctcg aaacatccga gatattgaaa aaaatctcgt ccagatgaat gagcgtaaac
     1321 aagagataga aaaacctcga cctaagcaag atcgaggtat ggattttggg atgtaattat
     1381 tttctagtat gttggtgttt atttcttagt attagcttgt actaatgttt aaatttagct
     1441 atattgaatg tagtataggc ttgtttatat attttggtgg tgttatgaat aataactgtg
     1501 tagtagtact aactgctcgt ggaaagacta gaattttaaa agaaggtggt agtcagtctt
     1561 ggagactaaa tgctcataat gcaggaaagt gcgattatct agtttgtgtg caaaatagga
     1621 aacaatcttg gggagaacca gaggctgatc atcacactgc atttttagtt ggcaaaatat
     1681 ctagtatcag ccaatctcaa gaagaagatg cagatggtag atggataata aatatctccg
     1741 aatatgctga tatatctatt cccaatatgt gggatggtaa tcggaatcca gtttcttata
     1801 gttcattaga agagatgaat atcgatttga atgagctgaa atttaccaaa ttaaatgaag
     1861 ataattcttc taaaacactg gataaaatat ctattcctga agctaagaga ctattagcta
     1921 attattttga tgtccctcaa gaaaatattt caattacaat caatatgtaa gcttttaatt
     1981 gaccaaaaaa agaaaaaagc ctttcacgtt aatgaaaggc ttttttgaat cttagcgctt
     2041 gggagggaag atcttagatc cgcagtctct caaacttaca actatatatt agggtatata
     2101 cggtggaatt gtcaaatgat gcagtgctta agatagagca ggctttatct ataccacgct
     2161 tatctaagta tgaaaatttc tataaagata aaggtgaacc atatgaaaaa tctgatgtat
     2221 tgatgcttta tgaacgtaat ttaataattt ccaataagtt tttttactta ttaaattatt
     2281 ttgaagttgt tctgagaaat gctgttgttc aggcaataga aatatctttt aggtgtaatg
     2341 aaacaaatag ttggcatgaa aatgaagctt ttattcgaag ccttagccgt agagggaggt
     2401 atagccctaa aagtatgttt gatagtgcta aagagaagtt cccagattcg cctagtaaaa
     2461 tgatccctga gcttaaattt gtattctggc aaaagatgct tatggctaat tatgaagaac
     2521 gtatttggca gggatgtttt aattctattt ttcctaatgc aacagttgaa aacaggcaaa
     2581 ttttttacga ttggacagat caattacgtg aattaagaaa caggattgct catcatgaac
     2641 cgattatttt taaccgtaat ctagaaaatg atttagaggt tttaacaaaa tttatttact
     2701 gtcgttgtat taatacatac gattttatcg agcaatctgc attagatcta aaagaatatt
     2761 tagattttta agataagaat ttcaccccaa acaatcgaaa ggtcagacta ttttgcaaat
     2821 gttgaattaa caaggttagt agtaaagcag taaatccgat aacggtagca gtgtttccta
     2881 acacctgagt taagccgcgc cgcaaagcgg cgtcggcttg gacgaattgt taggccgcat
     2941 atcgcgacct gaaagcggca cgcaagacct caaccttttc cgccccgagt gaggtgcatg
     3001 cgaacctgta ggactctatg tgctttgtag gccagtccac tggtggtact tcatcggcat
     3061 agtaaaagta atcccagatg atcgcctccc agctgttaca acggactggc cgcccggcga
     3121 tgacaccctc agccgcctct gggcacgagc cctgcggagc ctccgcgatt tcatacgctt
     3181 cgtctgccca ccaagcaggt tcgcagtcaa gtaactcatc cccgatctcc gctaagaatc
     3241 catagtccaa ctcctccgtg acgcgcccgc cgagcatttc aactattgcc tcgagctcgc
     3301 cgcgcctctc gccgggaaaa gtcagatcaa tatcatcgtg cttgcgtgtt acacgcccta
     3361 gccgtgcatc gatcgcccag cccccaccga tccagagcgg cagatttcgc tcatctgccg
     3421 cagctagaat ttggtgtatc aatgcgacct gcgttgtgtc catgcggcct aacatagtaa
     3481 gccctcccag aatgtcagta attagactat taattgaatt gttacatcca atagtgagct
     3541 cccagtgatt gtcttaacta tccaaccttt cgattggatg gggcagagca ccattattct
     3601 gtttgaatgc ggtgttagtg tcaactaggt tctttatttc ttcaaggagt ttgaaagtgc
     3661 taagtatttt tactttactt tgttcctcaa tataaatttt tttaagacca tttagcagct
     3721 tgtatttgga tattttggat aggagaatga ataaggttgc ggtattgatt cttccccctt
     3781 ttagatgaag taaacggttt ttttgcagta ttggaatatt tctatattct gttagttgat
     3841 cttttaagtc atcaggaatt ttcatttcgt aaaaattata ttttttagaa cagtctaata
     3901 tcgagtgatt taggctttct gtatttgtat taataaaaat gcatttttca aatgtgcaat
     3961 ttttaaagcg acaatctcta aaaatagcag cataaaatac gcactttttg aagtgaacat
     4021 tcgtaaacct attcttattt aagattactc ctgaaaaatc agataaaaca atttttatat
     4081 ttttgaatga acatgatcgt aggcgggatc ctctcaaatt aacattaaaa aatgttttat
     4141 tagagaaacg tactttagaa gaaatagatt tggctaggag gctatttgaa tacttacgtt
     4201 tttcgatgtg cttgttatga aatctaggaa gttttgacat taatagtcca aagaatagga
     4261 gtttcagctt aattcttagg tgtaatcaaa aaaaataatg gacaggtctc ttgcgaaatc
     4321 tgtccattag ttttaaattc gatgggtaca gttatttgat gcttgcaaca tcaattaacg
     4381 aaagatcggc agagcttgca actcatactg atattcacct taaaggctag taccactatc
     4441 tattttaaag aattagaaca gtttgacaag cctcaatttg aagtccttcg ttaattcagc
     4501 agtttaaatc atcaaataag cacctaacgg tcgagcttta tctacttctg tgcgcctttt
     4561 ttaaggaaga tatgcgttgc atgtggaggg aataagtgtc actggtgcat cgtagcgata
     4621 gtcgcaaggc taaaccgctt acaggggatc tcacataggc ggagcgttgg aagtagttgt
     4681 ataggttggt taagttgcat gttggagtaa tcctcgcaga gataccacca tacttcgaca
     4741 gggagcgcgt gggagaaggt gcaaaacctt tagtccttgt ctgcatggtc agcaaaacca
     4801 tgtgcggata cgaccgatgc gtggctccgt cagcccaatt acaggttaat cggcaatact
     4861 taagaggata tttgattttt ttaaatcaaa gcctcttagg gaaagtattg ccgaaactct
     4921 ctcaacgttc accatcagcc cgagcaaagc gagggatgtg ggacggtgag aaagagttga
     4981 gcgcatttgt ttttacaaga ttcatgccaa tacctactag agtttatata ccgtgtcatt
     5041 tgattagttt ttattgaatt tcactcgtcc atttttaaat aagcaaggat acacagctac
     5101 ccctatttaa cttcgttaaa cagtgatagt ctgtgacctt gatcaggggc ttcacccccg
     5161 atacccccga aaagctttaa acagaaaaat caaagatttt ccaatttaaa gcttacaaaa
     5221 gccaaagcag ggtagattga aatgtcagga caggaacaag aaaacaaaca gagcaaagca
     5281 gttcgagaac gaacctttaa agtcagactt tcagaatcag aatatcaatt gctcgattcc
     5341 aataatcctt atggttcgat tgcaaagttg cttagacaag cagcagtaga gaaagcgact
     5401 aaaattcata attccaaagc agaagatgac caaaaaatta aagcaacaaa ggttgttgaa
     5461 tcttcgtata gcaagacaga gcgagcatta attttagagc ttggtcggat tggcaacaat
     5521 gtgaatcaga ttgctaaagc agtgaatacc gatattgcag gggtaggtac ttttgacaaa
     5581 gtgaagttac ttcatttgtt gatttccata gatcagcagc ttcgggagtt acgtccagat
     5641 gatcgttgat ttctttagac atggttcagg tttatcaaaa ggctgtctcg attatttgtt
     5701 aggtgaagat cgagagcgtg aacatgctca agtgctcaat ggtgatgtag aactgacagc
     5761 acaactcatt gatagcagtc cttttgccaa aaaatatacc agtggttgtt tgtcctttta
     5821 tgagcatgat ttatctgacc aggataaaca gaaaatcatg caaaattttg aggaatgttt
     5881 attcccggga ctagataaag accaatatca aattttatgg gtacagcacc aagacaaaat
     5941 taatcaggac acaggtgaga caaggcttga gcttaatttt gtgattccta atgttgagct
     6001 ttcaacaggc aaacgcctac agccattcta tgcgccagtc gatttagatc gagtcgatct
     6061 atttaagcag atcacta
//
