LOCUS       NZ_CP061595             3857 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS60 plasmid pAS60-3, complete
            sequence.
ACCESSION   NZ_CP061595
VERSION     NZ_CP061595.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092236
            Assembly: GCF_014719835.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:23:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,184
            CDSs (total)                      :: 4,087
            Genes (coding)                    :: 3,933
            CDSs (with protein)               :: 3,933
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 83 of 154
            Pseudo Genes (incomplete)         :: 70 of 154
            Pseudo Genes (internal stop)      :: 21 of 154
            Pseudo Genes (multiple problems)  :: 16 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061595.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS60"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS60-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(2403..3857,1..447)
                     /locus_tag="IC789_RS20745"
                     /old_locus_tag="IC789_20735"
     CDS             join(2403..3857,1..447)
                     /locus_tag="IC789_RS20745"
                     /old_locus_tag="IC789_20735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            419..706
                     /locus_tag="IC789_RS21050"
     CDS             419..706
                     /locus_tag="IC789_RS21050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190977978.1"
                     /translation="MTGVIHLDFDTFFSAEHCPCNAVLEKKHLQRSHICDILSQYFVLK
                     ISHLTAIQKGVIFSSGEKKAGLATRFFLFVKQTLKASVKLKFDTCILLMF"
                     /name="hypothetical protein"
     gene            733..1695
                     /locus_tag="IC789_RS20750"
                     /old_locus_tag="IC789_20740"
     CDS             733..1695
                     /locus_tag="IC789_RS20750"
                     /old_locus_tag="IC789_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     gene            1960..2406
                     /locus_tag="IC789_RS20755"
                     /old_locus_tag="IC789_20745"
     CDS             1960..2406
                     /locus_tag="IC789_RS20755"
                     /old_locus_tag="IC789_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_308401008.1"
                     /translation="MKRKDTWRPLCYADVFHDLVQGQAPETPKSKGKSMTAIKRNKVYK
                     VRFSDAEVQKIKDANILNIAKYLRDTSLAQIDATQGNAKKKNQENHYTKLDRDFLLELS
                     RIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLKELKK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2404..3857
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.701"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acagaacaga accatagacg aattagagaa ctacaacagc aaactagtga agcactgcaa
       61 ccagttgaac gagaaagaaa acgagctgat caatatcatc aagaaactac agaatccgaa
      121 gctatcagag gatttattgc aaggcttgga gagcagctta aaacagcgat tacagagcca
      181 tttagagcaa ttagtgactg ggttaaatct aaagaacgag atcaacgaac tattgccaac
      241 gatgatctat acagaaatcg agaagcagac agcaccattg aacgaacagc ttcaaaggaa
      301 actagactca gtgcaacagt atcacgaaaa attcgagggt ttgatcaaac aaatatcttc
      361 aaggctttag atgaactgga cagacgtaga gagttgcaac tggagcaaca acgtaaaaat
      421 gacaggagtt attcacctag attttgatac ttttttttct gcggagcatt gcccttgcaa
      481 tgctgtcctc gaaaaaaagc atcttcaaag gtcacatatt tgcgacattt tgtcgcaata
      541 ttttgttttg aaaatcagtc atttgacagc aattcaaaaa ggtgtaatat tttcatcagg
      601 cgaaaaaaaa gcaggtttgg cgacccgctt ttttctcttt gttaaacaaa cgcttaaagc
      661 atccgtaaaa ctcaaattcg atacttgtat tttactgatg ttttgagcca atgcaacact
      721 tggacgtgtt tcatgctcaa taaaacgcaa ttttcagacg atttttacga aaaactccca
      781 aaaaaaccgt attgctcgga tgatttaggg cgtggggtga ttattcgccc aaaacgtacc
      841 gcaatccaga agccatacat tcagcataat cccccttgtc ttgttagctc gttggtgttc
      901 gatatagacc gccaagacgc ttattttgcg tggtctgacg caaatttacc gaccccaaca
      961 tggatagcca aaaatcgcca aaatggacat gcacatattg gctatatgct tttagctcca
     1021 gtgtgtacca cgcatcgagc aaagcagaat gtcatacagt accttgccaa gattgagcag
     1081 gcgtatagct tggcgttagg tgcagatcgt ggctatacgg ggttaatcac taaaaatcca
     1141 tgtcacggaa catgggaaaa tcataccttt ggcgttcaac catacgacct aaattattta
     1201 gccgattttg tagagcttca ggaactaaaa accgacttaa aagaagtttc agggcttggg
     1261 cgtaactgta tgatgttcga taccgttcgt ttttgggcgt atgaagcgat tagagcgttt
     1321 atttcggggt cgcctgatag ttggtatgcg gaagtgctaa aagtcgctat aaacgcaaat
     1381 ggtgcgtttt tagagccatt gccatattca gaggtaaagg caaccgctaa gtctattgct
     1441 cgttgggtat ggcgcaatca cacagaagca catgccaaat ttatcgagcg tcagactgtc
     1501 aaagggcgta aaggcggtct aagcagtgat tccagtcatg gcggtaaggc taggtcacag
     1561 caatacagtg atatgcgtca ggaagcctta aaactgcata tacagggcat aaagcagaaa
     1621 gatatagccc tacaattgaa tgtttcggat agaacaatca gaacgtggat aaaaaagcgg
     1681 aaagtgcaag cctaaatcag atatcagccc cctagggtgc ttttttccca tttcattttt
     1741 aaaaattatt attaaaggga atgagcaacg gctgtgggca aagtaaaacc ctgtgatcta
     1801 aacctgtgtg caactcaacg tataacggct ttatcgttat gcggagttgt gcatagtgtt
     1861 taggcacaga tcaggtttta cggtcgcttg cgattgtcca tagtcttgct atgacctgct
     1921 taatcttaat tgaattacgt ctaggggtct gctatcgtaa tgaaacgcaa ggatacatgg
     1981 agacccctct gctacgcaga tgtgttccat gaccttgttc aggggcaagc ccccgaaacc
     2041 cccaaaagca aaggcaaaag catgacagcg atcaaacgta ataaggttta caaagtccga
     2101 ttttctgatg cagaggtaca gaaaatcaaa gatgccaata tcctaaatat tgccaaatat
     2161 ctaagggata cttcgcttgc ccaaattgat gcaacccaag gcaacgccaa aaaaaagaat
     2221 caggaaaacc actacacaaa attagaccgt gactttttgc ttgagctatc ccgaattggg
     2281 ggcaacatca atcagattgc aaaggcaata aatactgata ttgcaagcag tcgtcccctt
     2341 gatgctgcaa ggcttttgca cctgttaata gggattgatc agaccttaaa ggaattgaaa
     2401 aaatgattgt tcaattcttt agatatggga acggtttaag taaaggtcca ctggattatt
     2461 tacttggtaa agaccgtgat cgagattatg caaaagtcct acagggcgat gtttccgaag
     2521 tatcaggatt aattgatacg tcaccttatg caaaaaaata tacatccggc tgcctaagtt
     2581 tttatgagca tgatttatct gaccaggata aacaaaaaat catgcaggat tttgagcaag
     2641 ccttattccc tggcatggat cagagccagt accgtgtctt gtggattgag catcaagaca
     2701 aattaaacga agaaacaggg gagcgaagat tagagcttaa cttcctaatc cctaatgttg
     2761 aaatccttac agggcaacgt ttacagcctt attatgataa agctgatcgt cctcgtattg
     2821 atctgtttaa gaagattaca aactatgaat atcaactgca tgatgcagat gaccccctct
     2881 atcgccaagc cgttacaacg gcaaaaaact tgcctaaaac tgttaatgaa atcaaggaaa
     2941 cgcttgatat tgaagcaacg agagccgttg aaggtggact aattacagat cggcagagca
     3001 tgaaaaaatg gctattggat cttggtcttg aagtcaccag agaaaccaag aaaagcctat
     3061 ccattaaaaa tccgaatgat gacgaaaaag cccgcccgat aaggcttaca ggtgcaatct
     3121 atgagcaaga ttttagactt acagaaacga gccaacaact tactatcgca gcatcagaac
     3181 gatatagacg agaagctaaa cagcgaaatc agtcagatat tcaaagatat gcggaataca
     3241 gtgcaaagcg aagcacagag cttgagcagc agtatagaca gcaccaaaac gtatatacag
     3301 cagaaacaga gcgaagcaac gaacgagata ttgcagcaga tcgaccagac tacagctcaa
     3361 accaagaagc agcagcaaca aggcatgaca gccctattgc aagaacaaca acagcagatc
     3421 aaacagctac aagcgaactt gcagcagttg aacagcttga gccaagaaac agcccaagca
     3481 gccaaattaa agaaaaccct tattacatcg aatatagcct tgatttcact ggcatgtata
     3541 acgcttatca gcagtatttg tctggggtac gtcagcaaga gcaaatacag cgacatcaca
     3601 gagatggaga acaaagtcca gtggttaaaa agccaagggg cgagcctgaa tattcagaag
     3661 tgtgggacag agaaaaaatg gaactggtgc gtccagatcg acccgaaagc gcagcagtac
     3721 agcaacaaca tgcgaatagt gcaggaaacc aattaaatga atctcgaagc acagttattg
     3781 cagattatcg agcagcaaca gcagcagctc aaagagcaac agaagcagca agagcaagcc
     3841 ttgcagctca ttcaggc
//
