LOCUS       NZ_CP061594             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS60 plasmid pAS60-2, complete
            sequence.
ACCESSION   NZ_CP061594
VERSION     NZ_CP061594.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092236
            Assembly: GCF_014719835.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:23:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,184
            CDSs (total)                      :: 4,087
            Genes (coding)                    :: 3,933
            CDSs (with protein)               :: 3,933
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 83 of 154
            Pseudo Genes (incomplete)         :: 70 of 154
            Pseudo Genes (internal stop)      :: 21 of 154
            Pseudo Genes (multiple problems)  :: 16 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061594.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS60"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS60-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(5997..6078,1..452))
                     /gene="aadB"
                     /locus_tag="IC789_RS20715"
                     /old_locus_tag="IC789_20705"
     CDS             complement(join(5997..6078,1..452))
                     /gene="aadB"
                     /locus_tag="IC789_RS20715"
                     /old_locus_tag="IC789_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(543..1229)
                     /locus_tag="IC789_RS20720"
                     /old_locus_tag="IC789_20710"
     CDS             complement(543..1229)
                     /locus_tag="IC789_RS20720"
                     /old_locus_tag="IC789_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            2232..2639
                     /locus_tag="IC789_RS20725"
                     /old_locus_tag="IC789_20715"
     CDS             2232..2639
                     /locus_tag="IC789_RS20725"
                     /old_locus_tag="IC789_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2629..4443
                     /locus_tag="IC789_RS20730"
                     /old_locus_tag="IC789_20720"
     CDS             2629..4443
                     /locus_tag="IC789_RS20730"
                     /old_locus_tag="IC789_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4552..5037
                     /locus_tag="IC789_RS20735"
                     /old_locus_tag="IC789_20725"
     CDS             4552..5037
                     /locus_tag="IC789_RS20735"
                     /old_locus_tag="IC789_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            5170..5838
                     /locus_tag="IC789_RS20740"
                     /old_locus_tag="IC789_20730"
     CDS             5170..5838
                     /locus_tag="IC789_RS20740"
                     /old_locus_tag="IC789_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     ARG             complement(4..464)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.0"
                     /coverage="87.01"
     Typing          2630..4440
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gactctatgt gctttgtagg ccagtccact ggtggtactt catcggcata gtaaaagtaa
       61 tcccagatga tcgcctccca gctgttacaa cggactggcc gcccggcgat gacaccctca
      121 gccgcctctg ggcacgagcc ctgcggagcc tccgcgattt catacgcttc gtctgcccac
      181 caagcaggtt cgcagtcaag taactcatcc ccgatctccg ctaagaatcc atagtccaac
      241 tcctccgtga cgcgcccgcc gagcatttca actattgcct cgagctcgcc gcgcctctcg
      301 ccgggaaaag tcagatcaat atcatcgtgc ttgcgtgtta cacgccctag ccgtgcatcg
      361 atcgcccagc ccccaccgat ccagagcggc agatttcgct catctgccgc agctagaatt
      421 tggtgtatca atgcgacctg cgttgtgtcc atgcggccta acatagtaag ccctcccaga
      481 atgtcagtaa ttagactatt aattgaattg ttacatccaa tagtgagctc ccagtgattg
      541 tcttaactat ccaacctttc gattggatgg ggcagagcac cattattctg tttgaatgcg
      601 gtgttagtgt caactaggtt ctttatttct tcaaggagtt tgaaagtgct aagtattttt
      661 actttacttt gttcctcaat ataaattttt ttaagaccat ttagcagctt gtatttggat
      721 attttggata ggagaatgaa taaggttgcg gtattgattc ttcccccttt tagatgaagt
      781 aaacggtttt tttgcagtat tggaatattt ctatattctg ttagttgatc ttttaagtca
      841 tcaggaattt tcatttcgta aaaattatat tttttagaac agtctaatat cgagtgattt
      901 aggctttctg tatttgtatt aataaaaatg catttttcaa atgtgcaatt tttaaagcga
      961 caatctctaa aaatagcagc ataaaatacg cactttttga agtgaacatt cgtaaaccta
     1021 ttcttattta agattactcc tgaaaaatca gataaaacaa tttttatatt tttgaatgaa
     1081 catgatcgta ggcgggatcc tctcaaatta acattaaaaa atgttttatt agagaaacgt
     1141 actttagaag aaatagattt ggctaggagg ctatttgaat acttacgttt ttcgatgtgc
     1201 ttgttatgaa atctaggaag ttttgacatt aatagtccaa agaataggag tttcagctta
     1261 attcttaggt gtaatcaaaa aaaataatgg acaggtctct tgcgaaatct gtccattagt
     1321 tttaaattcg atgggtacag ttatttgatg cttgcaacat caattaacga aagatcggca
     1381 gagcttgcaa ctcatactga tattcacctt aaaggctagt accactatct attttaaaga
     1441 attagaacag tttgacaagc ctcaatttga agtccttcgt taattcagca gtttaaatca
     1501 tcaaataagc acctaacggt cgagctttat ctacttctgt gcgccttttt taaggaagat
     1561 atgcgttgca tgtggaggga ataagtgtca ctggtgcatc gtagcgatag tcgcaaggct
     1621 aaaccgctta caggggatct cacataggcg gagcgttgga agtagttgta taggttggtt
     1681 aagttgcatg ttggagtaat cctcgcagag ataccaccat acttcgacag ggagcgcgtg
     1741 ggagaaggtg caaaaccttt agtccttgtc tgcatggtca gcaaaaccat gtgcggatac
     1801 gaccgatgcg tggctccgtc agcccaatta caggttaatc ggcaatactt aagaggatat
     1861 ttgatttttt taaatcaaag cctcttaggg aaagtattgc cgaaactctc tcaacgttca
     1921 ccatcagccc gagcaaagcg agggatgtgg ggacggtgag aaagagttga gcgcatttgt
     1981 ttttacaaga ttcatgccaa tacctactag agtttatata ccgtgtcatt tgattagttt
     2041 ttattgaatt tcactcgtcc atttttaaat aagcaaggat acacagctac ccctatttaa
     2101 cttcgttaaa cagtgatagt ctgtgacctt gatcaggggc ttcacccccg atacccccga
     2161 aaagctttaa acagaaaaat caaagatttt ccaatttaaa gcttacaaaa gccaaagcag
     2221 ggtagattga aatgtcagga caggaacaag aaaacaaaca gagcaaagca gttcgagaac
     2281 gaacctttaa agtcagactt tcagaatcag aatatcaatt gctcgattcc aataatcctt
     2341 atggttcgat tgcaaagttg cttagacaag cagcagtaga gaaagcgact aaaattcata
     2401 attccaaagc agaagatgac caaaaaatta aagcaacaaa ggttgttgaa tcttcgtata
     2461 gcaagacaga gcgagcatta attttagagc ttggtcggat tggcaacaat gtgaatcaga
     2521 ttgctaaagc agtgaatacc gatattgcag gggtaggtac ttttgacaaa gtgaagttac
     2581 ttcatttgtt gatttccata gatcagcagc ttcgggagtt acgtccagat gatcgttgat
     2641 ttctttagac atggttcagg tttatcaaaa ggctgtctcg attatttgtt aggtgaagat
     2701 cgagagcgtg aacatgctca agtgctcaat ggtgatgtag aactgacagc acaactcatt
     2761 gatagcagtc cttttgccaa aaaatatacc agtggttgtt tgtcctttta tgagcatgat
     2821 ttatctgacc aggataaaca gaaaatcatg caaaattttg aggaatgttt attcccggga
     2881 ctagataaag accaatatca aattttatgg gtacagcacc aagacaaaat taatcaggac
     2941 acaggtgaga caaggcttga gcttaatttt gtgattccta atgttgagct ttcaacaggc
     3001 aaacgcctac agccattcta tgcgccagtc gatttagatc gagtcgatct atttaagcag
     3061 atcactaata ccgaacattc actgtatgac cccgatgatc ctgagcatcg acagctattt
     3121 ttaaataaaa agaacctacc caaagatatt aaggatttta aagcgcagtt acatcaacgt
     3181 gtttaccgag cggttgccaa tggtgatgtt gccgatcggc aagaattggt gcaatggctt
     3241 gaatcgaatc aaatcaatgt cactcgacaa gtaaaaaact ccatatccat tgaaaaccca
     3301 tatgagggcg caaaacgtcc catccgttta gagggcgaga tttatgagca aggctttaga
     3361 gctactggcg aatatagaca agaagtacaa caacgaattg aagaatacag aggaacaact
     3421 tctgagcggt atcgaacaaa cgtcacagac tatcagagac agcttgaaca taagagccaa
     3481 tatcactgcg accgctatcc aacagtcgga cgagaaaata gcccagagca ttcaaaacaa
     3541 cgcccagacg gtcgagaagc agttaagcct gtatcaaacc ttgctgcaat cgaaattgag
     3601 ccatttaacg caattaaacg agcaaataca gagccaagaa caacaagccc agaatcaagc
     3661 ggtacagaaa aaacttacca ctttgaatat gggactgatt tcagtagcag ttattttgct
     3721 tatagcaatt ttctcgcttg gtctcgtcac caaaaacaag tacagcgaga tgcagacgct
     3781 aaataccgtg atcaacgaga agcaaatgag ggtcgaccat ttgaaccgat cgggaggcag
     3841 tttgacgatc agcacatgca agcgaccaga cagcaaagca cagcgcctat gtattcagat
     3901 caacaagaac gcaggcgaat ggcagaacgg ctacatgatt ccaatggggt attaaacgat
     3961 gaccgaatta gaaacacaat tattgagaat catcgaagaa caacagcaac aattaatgca
     4021 acaacaagtg caattgcaga agcaacagca agctttagac acaatgcaaa ccaaaattat
     4081 ccaagccttg tcagcacaat caaagacaat caagagcgat cagcaaagct tacagcaagc
     4141 acagaaatca ttagcgacaa tgcagaagca atttcacgag ctagaacgaa gtacagcaag
     4201 ctataccgag cagatcaatg gcaaagtccg agagatgagc cttatcacag ccaacaacga
     4261 agaaattctg gagagaatct caaccacaca gcattctgtc tcgtcagaag tagccaaagc
     4321 gcagcagaat ttgctcgaaa catccgagat attgaaaaaa atctcgtcca gatgaatgag
     4381 cgtaaacaag agatagaaaa acctcgacct aagcaagatc gaggtatgga ttttgggatg
     4441 taattatttt ctagtatgtt ggtgtttatt tcttagtatt agcttgtact aatgtttaaa
     4501 tttagctata ttgaatgtag tataggcttg tttatatatt ttggtggtgt tatgaataat
     4561 aactgtgtag tagtactaac tgctcgtgga aagactagaa ttttaaaaga aggtggtagt
     4621 cagtcttgga gactaaatgc tcataatgca ggaaagtgcg attatctagt ttgtgtgcaa
     4681 aataggaaac aatcttgggg agaaccagag gctgatcatc acactgcatt tttagttggc
     4741 aaaatatcta gtatcagcca atctcaagaa gaagatgcag atggtagatg gataataaat
     4801 atctccgaat atgctgatat atctattccc aatatgtggg atggtaatcg gaatccagtt
     4861 tcttatagtt cattagaaga gatgaatatc gatttgaatg agctgaaatt taccaaatta
     4921 aatgaagata attcttctaa aacactggat aaaatatcta ttcctgaagc taagagacta
     4981 ttagctaatt attttgatgt ccctcaagaa aatatttcaa ttacaatcaa tatgtaagct
     5041 tttaattgac caaaaaaaga aaaaagcctt tcacgttaat gaaaggcttt tttgaatctt
     5101 agcgcttggg agggaagatc ttagatccgc agtctctcaa acttacaact atatattagg
     5161 gtatatacgg tggaattgtc aaatgatgca gtgcttaaga tagagcaggc tttatctata
     5221 ccacgcttat ctaagtatga aaatttctat aaagataaag gtgaaccata tgaaaaatct
     5281 gatgtattga tgctttatga acgtaattta ataatttcca ataagttttt ttacttatta
     5341 aattattttg aagttgttct gagaaatgct gttgttcagg caatagaaat atcttttagg
     5401 tgtaatgaaa caaatagttg gcatgaaaat gaagctttta ttcgaagcct tagccgtaga
     5461 gggaggtata gccctaaaag tatgtttgat agtgctaaag agaagttccc agattcgcct
     5521 agtaaaatga tccctgagct taaatttgta ttctggcaaa agatgcttat ggctaattat
     5581 gaagaacgta tttggcaggg atgttttaat tctatttttc ctaatgcaac agttgaaaac
     5641 aggcaaattt tttacgattg gacagatcaa ttacgtgaat taagaaacag gattgctcat
     5701 catgaaccga ttatttttaa ccgtaatcta gaaaatgatt tagaggtttt aacaaaattt
     5761 atttactgtc gttgtattaa tacatacgat tttatcgagc aatctgcatt agatctaaaa
     5821 gaatatttag atttttaaga taagaatttc accccaaaca atcgaaaggt cagactattt
     5881 tgcaaatgtt gaattaacaa ggttagtagt aaagcagtaa atccgataac ggtagcagtg
     5941 tttcctaaca cctgagttaa gccgcgccgc aaagcggcgt cggcttggac gaattgttag
     6001 gccgcatatc gcgacctgaa agcggcacgc aagacctcaa ccttttccgc cccgagtgag
     6061 gtgcatgcga acctgtag
//
