LOCUS       NZ_CP061588             3855 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS62 plasmid pAS62-6, complete
            sequence.
ACCESSION   NZ_CP061588
VERSION     NZ_CP061588.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092238
            Assembly: GCF_014719435.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:32:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,288
            Genes (coding)                    :: 4,122
            CDSs (with protein)               :: 4,122
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 87 of 166
            Pseudo Genes (incomplete)         :: 75 of 166
            Pseudo Genes (internal stop)      :: 24 of 166
            Pseudo Genes (multiple problems)  :: 16 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061588.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS62"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS62-6"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(3418..3855,1..1464))
                     /locus_tag="IC791_RS21800"
                     /old_locus_tag="IC791_21785"
     CDS             complement(join(3418..3855,1..1464))
                     /locus_tag="IC791_RS21800"
                     /old_locus_tag="IC791_21785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1461..1610)
                     /gene="mobC"
                     /locus_tag="IC791_RS22075"
     CDS             complement(1461..1610)
                     /gene="mobC"
                     /locus_tag="IC791_RS22075"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_223861193.1"
                     /translation="MLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLK
                     ELKK"
                     /name="mobC"
     gene            complement(1622..1804)
                     /locus_tag="IC791_RS22080"
     CDS             complement(1622..1804)
                     /locus_tag="IC791_RS22080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223861277.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLAQIDAT
                     QGNAKKRIRKTTTQN"
                     /name="hypothetical protein"
     gene            complement(2170..3132)
                     /locus_tag="IC791_RS21810"
                     /old_locus_tag="IC791_21795"
     CDS             complement(2170..3132)
                     /locus_tag="IC791_RS21810"
                     /old_locus_tag="IC791_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     Typing          complement(2..1464)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.516"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgttctgtg cctgaatgag ctgcaaggct tgctcttgct gcttctgttg ctctttgagc
       61 tgctgctgtt gctgctcgat aatctgcaat aactgtgctt cgagattcat ttaattggtt
      121 tcctgcacta ttcgcatgtt gttgctgtac tgctgcgctt tcgggtcgat ctggacgcac
      181 cagttccatt ttttctctgt cccacacttc tgaatattca ggctcgcccc ttggcttttt
      241 aaccactgga ctttgttctc catctctgtg atgtcgctgt atttgctctt gctgacgtac
      301 cccagacaaa tactgctgat aagcgttata catgccagtg aaatcaaggc tatattcgat
      361 gtaataaggg ttttctttaa tttggctgct tgggctgttt cttggctcaa gctgttcaac
      421 tgctgcaagt tcgcttgtag ctgtttgatc tgctgttgtt gttcttgcaa tagggctgtc
      481 atgccttgtt gctgctgctt cttggtttga gctgtagtct ggtcgatctg ctgcaatatc
      541 tcgttcgttg cttcgctctg tttctgctgt atatacgttt tggtgctgtc tatactgctg
      601 ctcaagctct gtgcttcgct ttgcactgta ttccgcatat ctttgaatat ctgactgatt
      661 tcgctgttta gcttctcgtc tatatcgttc tgatgctgcg atagtaagtt gttggctcgt
      721 ttctgtaagt ctaaaatctt gctcatagat tgcacctgta agccttatcg ggcgggcttt
      781 ttcgtcatca ttcggatttt taatggatag gcttttcttg gtttctctgg tgacttcaag
      841 accaagatcc aatagccatt ttttcatgct ctgccgatct gtaattagtc caccttcaac
      901 ggctctcgtt gcttcaatat caagcgtttc cttgatttca ttaacagttt taggcaagtt
      961 ttttgccgtt gtaacggctt ggcgatagag ggggtcatct gcatcatgca gttgatattc
     1021 atagtttgta atcttcttaa acagatcaat acgaggacga tcagctttat cataataagg
     1081 ctgtaaacgt tgccctgtaa ggatttcaac attagggatt aggaagttaa gctctaatct
     1141 tcgctcccct gtttcttcgt ttaatttgtc ttgatgctca atccacaaga cacggtactg
     1201 gctctgatcc atgccaggga ataaggcttg ctcaaaatcc tgcatgattt tttgtttatc
     1261 ctggtcagat aaatcatgct cataaaaact taggcagccg gatgtatatt tttttgcata
     1321 aggtgacgta tcaattaatc ctgatacttc ggaaacatcg ccctgtagga cttttgcata
     1381 atctcgatca cggtctttac caagtaaata atccagtgga cctttactta aaccgttccc
     1441 atatctaaag aattgaacaa tcattttttc aattccttta aggtctgatc aatccctatt
     1501 aacaggtgca aaagccttgc agcatcaagg ggacgactgc ttgcaatatc agtatttatt
     1561 gcctttgcaa tctgattgat gttgccccca attcgggata gctcaagcaa aaagtcacgg
     1621 tctaattttg tgtagtggtt ttcctgattc tttttttggc gttgccttgg gttgcatcaa
     1681 tttgggcaag cgaagtatcc cttagatatt tggcaatatt taggatattg gcatctttga
     1741 ttttctgtac ctctgcatca gaaaatcgga ctttgtaaac cttattacgt ttgatcgctg
     1801 tcatgctttt gcctttgctt ttgggggttc gggggcttgc ccctgaacaa ggtcatggaa
     1861 cacatctgcg tagcagaggg gtctccatgt atccttgcgt ttcattacga tagcagaccc
     1921 ctagacgtaa ttcaattaag attaagcagg tcatagcaag actatggaca atcgcaagcg
     1981 accgtaaaac ctgatctgtg cctaaacact atgcacaact ccgcataacg ataaagccgt
     2041 tatacgttga gttgcacaca ggtttagatc acagggtttt actttgccca cagccgttgc
     2101 tcattccctt taataataat ttttaaaaat gaaatgggaa aaaagcaccc tagggggctg
     2161 atatctgatt taggcttgca ctttccgctt ttttatccac gttctgattg ttctatccga
     2221 aacattcaat tgtagggcta tatctttctg ctttatgccc tgtatatgca gttttaaggc
     2281 ttcctgacgc atatcactgt attgctgtga cctagcctta ccgccatgac tggaatcact
     2341 gcttagaccg cctttacgcc ctttgacagt ctgacgctcg ataaatttgg catgtgcttc
     2401 tgtgtgattg cgccataccc aacgagcaat agacttagcg gttgccttta cctctgaata
     2461 tggcaatggc tctaaaaacg caccatttgc gtttatagcg acttttagca cttccgcata
     2521 ccaactatca ggcgaccccg aaataaacgc tctaatcgct tcatacgccc aaaaacgaac
     2581 ggtatcgaac atcatacagt tacgcccaag ccctgaaact tcttttaagt cggtttttag
     2641 ttcctgaagc tctacaaaat cggctaaata atttaggtcg tatggttgaa cgccaaaggt
     2701 atgattttcc catgttccgt gacatggatt tttagtgatt aaccccgtat agccacgatc
     2761 tgcacctaac gccaagctat acgcctgctc aatcttggca aggtactgta tgacattctg
     2821 ctttgctcga tgcgtggtac acactggagc taaaagcata tagccaatat gtgcatgtcc
     2881 attttggcga tttttggcta tccatgttgg ggtcggtaaa tttgcgtcag accacgcaaa
     2941 ataagcgtct tggcggtcta tatcgaacac caacgagcta acaagacaag ggggattatg
     3001 ctgaatgtat ggcttctgga ttgcggtacg ttttgggcga ataatcaccc cacgccctaa
     3061 atcatccgag caatacggtt tttttgggag tttttcgtaa aaatcgtctg aaaattgcgt
     3121 tttattgagc atgaaacacg tccaagtgtt gcattggctc aaaacatcag taaaatacaa
     3181 gtatcgaatt tgagttttac ggatgcttta agcgtttgtt taacaaagag aaaaaagcgg
     3241 gtcgccaaac ctgctttttt ttcgcctgat gaaaatatta cacctttttg aattgctgtc
     3301 aaatgactga ttttcaaaac aaaatattgc gacaaaatgt cgcaaatatg tgacctttga
     3361 agatgctttt tttcgaggac agcattgcaa gggcaatgct ccgcagaaaa aaaagtatca
     3421 aaatctaggt gaataactcc tgtcattttt acgttgttgc tccagttgca actctctacg
     3481 tctgtccagt tcatctaaag ccttgaagat atttgtttga tcaaaccctc gaatttttcg
     3541 tgatactgtt gcactgagtc tagtttcctt tgaagctgtt cgttcaatgg tgctgtctgc
     3601 ttctcgattt ctgtatagat catcgttggc aatagttcgt tgatctcgtt ctttagattt
     3661 aacccagtca ctaattgctc taaatggctc tgtaatcgct gttttaagct gctctccaag
     3721 ccttgcaata aatcctctga tagcttcgga ttctgtagtt tcttgatgat attgatcagc
     3781 tcgttttctt tctcgttcaa ctggttgcag tgcttcacta gtttgctgtt gtagttctct
     3841 aattcgtcta tggtt
//
