LOCUS       NZ_CP061587             6077 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS62 plasmid pAS62-5, complete
            sequence.
ACCESSION   NZ_CP061587
VERSION     NZ_CP061587.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092238
            Assembly: GCF_014719435.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:32:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,288
            Genes (coding)                    :: 4,122
            CDSs (with protein)               :: 4,122
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 87 of 166
            Pseudo Genes (incomplete)         :: 75 of 166
            Pseudo Genes (internal stop)      :: 24 of 166
            Pseudo Genes (multiple problems)  :: 16 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061587.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS62"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS62-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5831..6077,1..1568)
                     /locus_tag="IC791_RS21770"
                     /old_locus_tag="IC791_21755"
     CDS             join(5831..6077,1..1568)
                     /locus_tag="IC791_RS21770"
                     /old_locus_tag="IC791_21755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1677..2162
                     /locus_tag="IC791_RS21775"
                     /old_locus_tag="IC791_21760"
     CDS             1677..2162
                     /locus_tag="IC791_RS21775"
                     /old_locus_tag="IC791_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            2295..2963
                     /locus_tag="IC791_RS21780"
                     /old_locus_tag="IC791_21765"
     CDS             2295..2963
                     /locus_tag="IC791_RS21780"
                     /old_locus_tag="IC791_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3122..3655)
                     /gene="aadB"
                     /locus_tag="IC791_RS21785"
                     /old_locus_tag="IC791_21770"
     CDS             complement(3122..3655)
                     /gene="aadB"
                     /locus_tag="IC791_RS21785"
                     /old_locus_tag="IC791_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3746..4432)
                     /locus_tag="IC791_RS21790"
                     /old_locus_tag="IC791_21775"
     CDS             complement(3746..4432)
                     /locus_tag="IC791_RS21790"
                     /old_locus_tag="IC791_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5434..5841
                     /locus_tag="IC791_RS21795"
                     /old_locus_tag="IC791_21780"
     CDS             5434..5841
                     /locus_tag="IC791_RS21795"
                     /old_locus_tag="IC791_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(3126..3655)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(3123..3667)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          4..1565
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5832..6076
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="1.35e-47"
ORIGIN
        1 cgggactaga taaagaccaa tatcaaattt tatgggtaca gcaccaagac aaaattaatc
       61 aggacacagg tgagacaagg cttgagctta attttgtgat tcctaatgtt gagctttcaa
      121 caggcaaacg cctacagcca ttctatgcgc cagtcgattt agatcgagtc gatctattta
      181 agcagatcac taataccgaa cattcactgt atgaccccga tgatcctgag catcgacagc
      241 tatttttaaa taaaaagaac ctacccaaag atattaagga ttttaaagcg cagttacatc
      301 aacgtgttta ccgagcggtt gccaatggtg atgttgccga tcggcaagaa ttggtgcaat
      361 ggcttgaatc gaatcaaatc aatgtcactc gacaagtaaa aaactccata tccattgaaa
      421 acccatatga gggcgcaaaa cgtcccatcc gtttagaggg cgagatttat gagcaaggct
      481 ttagagctac tggcgaatat agacaagaag tacaacaacg aattgaagaa tacagaggaa
      541 caacttctga gcggtatcga acaaacgtca cagactatca gagacagctt gaacataaga
      601 gccaatatca ctgcgaccgc tatccaacag tcggacgaga aaatagccca gagcattcaa
      661 aacaacgccc agacggtcga gaagcagtta agcctgtatc aaaccttgct gcaatcgaaa
      721 ttgagccatt taacgcaatt aaacgagcaa atacagagcc aagaacaaca agcccagaat
      781 caagcggtac agaaaaaact taccactttg aatatgggac tgatttcagt agcagttatt
      841 ttgcttatag caattttctc gcttggtctc gtcaccaaaa acaagtacag cgagatgcag
      901 acgctaaata ccgtgatcaa cgagaagcaa atgagggtcg accatttgaa ccgatcggga
      961 ggcagtttga cgatcagcac atgcaagcga ccagacagca aagcacagcg cctatgtatt
     1021 cagatcaaca agaacgcagg cgaatggcag aacggctaca tgattccaat ggggtattaa
     1081 acgatgaccg aattagaaac acaattattg agaatcatcg aagaacaaca gcaacaatta
     1141 atgcaacaac aagtgcaatt gcagaagcaa cagcaagctt tagacacaat gcaaaccaaa
     1201 attatccaag ccttgtcagc acaatcaaag acaatcaaga gcgatcagca aagcttacag
     1261 caagcacaga aatcattagc gacaatgcag aagcaatttc acgagctaga acgaagtaca
     1321 gcaagctata ccgagcagat caatggcaaa gtccgagaga tgagccttat cacagccaac
     1381 aacgaagaaa ttctggagag aatctcaacc acacagcatt ctgtctcgtc agaagtagcc
     1441 aaagcgcagc agaatttgct cgaaacatcc gagatattga aaaaaatctc gtccagatga
     1501 atgagcgtaa acaagagata gaaaaacctc gacctaagca agatcgaggt atggattttg
     1561 ggatgtaatt attttctagt atgttggtgt ttatttctta gtattagctt gtactaatgt
     1621 ttaaatttag ctatattgaa tgtagtatag gcttgtttat atattttggt ggtgttatga
     1681 ataataactg tgtagtagta ctaactgctc gtggaaagac tagaatttta aaagaaggtg
     1741 gtagtcagtc ttggagacta aatgctcata atgcaggaaa gtgcgattat ctagtttgtg
     1801 tgcaaaatag gaaacaatct tggggagaac cagaggctga tcatcacact gcatttttag
     1861 ttggcaaaat atctagtatc agccaatctc aagaagaaga tgcagatggt agatggataa
     1921 taaatatctc cgaatatgct gatatatcta ttcccaatat gtgggatggt aatcggaatc
     1981 cagtttctta tagttcatta gaagagatga atatcgattt gaatgagctg aaatttacca
     2041 aattaaatga agataattct tctaaaacac tggataaaat atctattcct gaagctaaga
     2101 gactattagc taattatttt gatgtccctc aagaaaatat ttcaattaca atcaatatgt
     2161 aagcttttaa ttgaccaaaa aaagaaaaaa gcctttcacg ttaatgaaag gcttttttga
     2221 atcttagcgc ttgggaggga agatcttaga tccgcagtct ctcaaactta caactatata
     2281 ttagggtata tacggtggaa ttgtcaaatg atgcagtgct taagatagag caggctttat
     2341 ctataccacg cttatctaag tatgaaaatt tctataaaga taaaggtgaa ccatatgaaa
     2401 aatctgatgt attgatgctt tatgaacgta atttaataat ttccaataag tttttttact
     2461 tattaaatta ttttgaagtt gttctgagaa atgctgttgt tcaggcaata gaaatatctt
     2521 ttaggtgtaa tgaaacaaat agttggcatg aaaatgaagc ttttattcga agccttagcc
     2581 gtagagggag gtatagccct aaaagtatgt ttgatagtgc taaagagaag ttcccagatt
     2641 cgcctagtaa aatgatccct gagcttaaat ttgtattctg gcaaaagatg cttatggcta
     2701 attatgaaga acgtatttgg cagggatgtt ttaattctat ttttcctaat gcaacagttg
     2761 aaaacaggca aattttttac gattggacag atcaattacg tgaattaaga aacaggattg
     2821 ctcatcatga accgattatt tttaaccgta atctagaaaa tgatttagag gttttaacaa
     2881 aatttattta ctgtcgttgt attaatacat acgattttat cgagcaatct gcattagatc
     2941 taaaagaata tttagatttt taagataaga atttcacccc aaacaatcga aaggtcagac
     3001 tattttgcaa atgttgaatt aacaaggtta gtagtaaagc agtaaatccg ataacggtag
     3061 cagtgtttcc taacacctga gttaagccgc gccgcaaagc ggcgtcggct tggacgaatt
     3121 gttaggccgc atatcgcgac ctgaaagcgg cacgcaagac ctcaaccttt tccgccccga
     3181 gtgaggtgca tgcgaacctg taggactcta tgtgctttgt aggccagtcc actggtggta
     3241 cttcatcggc atagtaaaag taatcccaga tgatcgcctc ccagctgtta caacggactg
     3301 gccgcccggc gatgacaccc tcagccgcct ctgggcacga gccctgcgga gcctccgcga
     3361 tttcatacgc ttcgtctgcc caccaagcag gttcgcagtc aagtaactca tccccgatct
     3421 ccgctaagaa tccatagtcc aactcctccg tgacgcgccc gccgagcatt tcaactattg
     3481 cctcgagctc gccgcgcctc tcgccgggaa aagtcagatc aatatcatcg tgcttgcgtg
     3541 ttacacgccc tagccgtgca tcgatcgccc agcccccacc gatccagagc ggcagatttc
     3601 gctcatctgc cgcagctaga atttggtgta tcaatgcgac ctgcgttgtg tccatgcggc
     3661 ctaacatagt aagccctccc agaatgtcag taattagact attaattgaa ttgttacatc
     3721 caatagtgag ctcccagtga ttgtcttaac tatccaacct ttcgattgga tggggcagag
     3781 caccattatt ctgtttgaat gcggtgttag tgtcaactag gttctttatt tcttcaagga
     3841 gtttgaaagt gctaagtatt tttactttac tttgttcctc aatataaatt tttttaagac
     3901 catttagcag cttgtatttg gatattttgg ataggagaat gaataaggtt gcggtattga
     3961 ttcttccccc ttttagatga agtaaacggt ttttttgcag tattggaata tttctatatt
     4021 ctgttagttg atcttttaag tcatcaggaa ttttcatttc gtaaaaatta tattttttag
     4081 aacagtctaa tatcgagtga tttaggcttt ctgtatttgt attaataaaa atgcattttt
     4141 caaatgtgca atttttaaag cgacaatctc taaaaatagc agcataaaat acgcactttt
     4201 tgaagtgaac attcgtaaac ctattcttat ttaagattac tcctgaaaaa tcagataaaa
     4261 caatttttat atttttgaat gaacatgatc gtaggcggga tcctctcaaa ttaacattaa
     4321 aaaatgtttt attagagaaa cgtactttag aagaaataga tttggctagg aggctatttg
     4381 aatacttacg tttttcgatg tgcttgttat gaaatctagg aagttttgac attaatagtc
     4441 caaagaatag gagtttcagc ttaattctta ggtgtaatca aaaaaaataa tggacaggtc
     4501 tcttgcgaaa tctgtccatt agttttaaat tcgatgggta cagttatttg atgcttgcaa
     4561 catcaattaa cgaaagatcg gcagagcttg caactcatac tgatattcac cttaaaggct
     4621 agtaccacta tctattttaa agaattagaa cagtttgaca agcctcaatt tgaagtcctt
     4681 cgttaattca gcagtttaaa tcatcaaata agcacctaac ggtcgagctt tatctacttc
     4741 tgtgcgcctt ttttaaggaa gatatgcgtt gcatgtggag ggaataagtg tcactggtgc
     4801 atcgtagcga tagtcgcaag gctaaaccgc ttacagggga tctcacatag gcggagcgtt
     4861 ggaagtagtt gtataggttg gttaagttgc atgttggagt aatcctcgca gagataccac
     4921 catacttcga cagggagcgc gtgggagaag gtgcaaaacc tttagtcctt gtctgcatgg
     4981 tcagcaaaac catgtgcgga tacgaccgat gcgtggctcc gtcagcccaa ttacaggtta
     5041 atcggcaata cttaagagga tatttgattt ttttaaatca aagcctctta gggaaagtat
     5101 tgccgaactc tctcaacgtt caccatcagc ccgagcaaag cgagggatgt ggggacggtg
     5161 agaaagagtt gagcgcattt gtttttacaa gattcatgcc aatacctact agagtttata
     5221 taccgtgtca tttgattagt ttttattgaa tttcactcgt ccatttttaa ataagcaagg
     5281 atacacagct acccctattt aacttcgtta aacagtgata gtctgtgacc ttgatcaggg
     5341 gcttcacccc cgataccccc gaaaagcttt aaacagaaaa atcaaagatt ttccaattta
     5401 aagcttacaa aagccaaagc agggtagatt gaaatgtcag gacaggaaca agaaaacaaa
     5461 cagagcaaag cagttcgaga acgaaccttt aaagtcagac tttcagaatc agaatatcaa
     5521 ttgctcgatt ccaataatcc ttatggttcg attgcaaagt tgcttagaca agcagcagta
     5581 gagaaagcga ctaaaattca taattccaaa gcagaagatg accaaaaaat taaagcaaca
     5641 aaggttgttg aatcttcgta tagcaagaca gagcgagcat taattttaga gcttggtcgg
     5701 attggcaaca atgtgaatca gattgctaaa gcagtgaata ccgatattgc aggggtaggt
     5761 acttttgaca aagtgaagtt acttcatttg ttgatttcca tagatcagca gcttcgggag
     5821 ttacgtccag atgatcgttg atttctttag acatggttca ggtttatcaa aaggctgtct
     5881 cgattatttg ttaggtgaag atcgagagcg tgaacatgct caagtgctca atggtgatgt
     5941 agaactgaca gcacaactca ttgatagcag tccttttgcc aaaaaatata ccagtggttg
     6001 tttgtccttt tatgagcatg atttatctga ccaggataaa cagaaaatca tgcaaaattt
     6061 tgaggaatgt ttattcc
//
