LOCUS       NZ_CP061581             3856 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS63 plasmid pAS63-3, complete
            sequence.
ACCESSION   NZ_CP061581
VERSION     NZ_CP061581.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092239
            Assembly: GCF_014719215.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:23:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,131
            CDSs (total)                      :: 4,034
            Genes (coding)                    :: 3,880
            CDSs (with protein)               :: 3,880
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 83 of 154
            Pseudo Genes (incomplete)         :: 72 of 154
            Pseudo Genes (internal stop)      :: 23 of 154
            Pseudo Genes (multiple problems)  :: 20 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061581.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS63"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS63-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(3847..3856,1..335))
                     /gene="mobC"
                     /locus_tag="IC792_RS20520"
                     /old_locus_tag="IC792_20510"
     CDS             complement(join(3847..3856,1..335))
                     /gene="mobC"
                     /locus_tag="IC792_RS20520"
                     /old_locus_tag="IC792_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_044103698.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLAQIDAT
                     QGNAKKKNQENHYTKLDRDFLLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIG
                     IDQTLKELKK"
                     /name="mobC"
     gene            complement(701..1663)
                     /locus_tag="IC792_RS20525"
                     /old_locus_tag="IC792_20515"
     CDS             complement(701..1663)
                     /locus_tag="IC792_RS20525"
                     /old_locus_tag="IC792_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     gene            complement(1949..3850)
                     /locus_tag="IC792_RS20535"
                     /old_locus_tag="IC792_20525"
     CDS             complement(1949..3850)
                     /locus_tag="IC792_RS20535"
                     /old_locus_tag="IC792_20525"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(1956..3850)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.575"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aattccttta aggtctgatc aatccctatt aacaggtgca aaagccttgc agcatcaagg
       61 ggacgactgc ttgcaatatc agtatttatt gcctttgcaa tctgattgat gttgccccca
      121 attcgggata gctcaagcaa aaagtcacgg tctaattttg tgtagtggtt ttcctgattc
      181 ttttttttgg cgttgccttg ggttgcatca atttgggcaa gcgaagtatc ccttagatat
      241 ttggcaatat ttaggatatt ggcatctttg attttctgta cctctgcatc agaaaatcgg
      301 actttgtaaa ccttattacg tttgatcgct gtcatgcttt tgcctttgct tttgggggtt
      361 cgggggcttg cccctgaaca aggtcatgga acacatctgc gtagcagagg ggtctccatg
      421 tatccttgcg tttcattacg atagcagacc cctagacgta attcaattaa gattaagcag
      481 gtcatagcaa gactatggac aatcgcaagc gaccgtaaaa cctgatctgt gcctaaacac
      541 tatgcacaac tccgcataac gataaagccg ttatacgttg agttgcacac aggtttagat
      601 cacagggttt tactttgccc acagccgttg ctcattccct ttaataataa tttttaaaaa
      661 tgaaatggga aaaaagcacc ctagggggct gatatctgat ttaggcttgc actttccgct
      721 tttttatcca cgttctgatt gttctatccg aaacattcaa ttgtagggct atatctttct
      781 gctttatgcc ctgtatatgc agttttaagg cttcctgacg catatcactg tattgctgtg
      841 acctagcctt accgccatga ctggaatcac tgcttagacc gcctttacgc cctttgacag
      901 tctgacgctc gataaatttg gcatgtgctt ctgtgtgatt gcgccatacc caacgagcaa
      961 tagacttagc ggttgccttt acctctgaat atggcaatgg ctctaaaaac gcaccatttg
     1021 cgtttatagc gacttttagc acttccgcat accaactatc aggcgacccc gaaataaacg
     1081 ctctaatcgc ttcatacgcc caaaaacgaa cggtatcgaa catcatacag ttacgcccaa
     1141 gccctgaaac ttcttttaag tcggttttta gttcctgaag ctctacaaaa tcggctaaat
     1201 aatttaggtc gtatggttga acgccaaagg tatgattttc ccatgttccg tgacatggat
     1261 ttttagtgat taaccccgta tagccacgat ctgcacctaa cgccaagcta tacgcctgct
     1321 caatcttggc aaggtactgt atgacattct gctttgctcg atgcgtggta cacactggag
     1381 ctaaaagcat atagccaata tgtgcatgtc cattttggcg atttttggct atccatgttg
     1441 gggtcggtaa atttgcgtca gaccacgcaa aataagcgtc ttggcggtct atatcgaaca
     1501 ccaacgagct aacaagacaa gggggattat gctgaatgta tggcttctgg attgcggtac
     1561 gttttgggcg aataatcacc ccacgcccta aatcatccga gcaatacggt ttttttggga
     1621 gtttttcgta aaaatcgtct gaaaattgcg ttttattgag catgaaacac gtccaagtgt
     1681 tgcattggct caaaacatca gtaaaataca agtatcgaat ttgagtttta cggatgcttt
     1741 aagcgtttgt ttaacaaaga gaaaaaagcg ggtcgccaaa cctgcttttt tttcgcctga
     1801 tgaaaatatt acaccttttt gaattgctgt caaatgactg attttcaaaa caaaatattg
     1861 cgacaaaatg tcgcaaatat gtgacctttg aagatgcttt ttttcgagga cagcattgca
     1921 agggcaatgc tccgcagaaa aaaaagtatc aaaatctagg tgaataactc ctgtcatttt
     1981 tacgttgttg ctccagttgc aactctctac gtctgtccag ttcatctaaa gccttgaaga
     2041 tatttgtttg atcaaaccct cgaatttttc gtgatactgt tgcactgagt ctagtttcct
     2101 ttgaagctgt tcgttcaatg gtgctgtctg cttctcgatt tctgtataga tcatcgttgg
     2161 caatagttcg ttgatctcgt tctttagatt taacccagtc actaattgct ctaaatggct
     2221 ctgtaatcgc tgttttaagc tgctctccaa gccttgcaat aaatcctctg atagcttcgg
     2281 attctgtagt ttcttgatga tattgatcag ctcgttttct ttctcgttca actggttgca
     2341 gtgcttcact agtttgctgt tgtagttctc taattcgtct atggttctgt tctgtgcctg
     2401 aatgagctgc aaggcttgct cttgctgctt ctgttgctct ttgagctgct gctgttgctg
     2461 ctcgataatc tgcaataact gtgcttcgag attcatttaa ttggtttcct gcactattcg
     2521 catgttgttg ctgtactgct gcgctttcgg gtcgatctgg acgcaccagt tccatttttt
     2581 ctctgtccca cacttctgaa tattcaggct cgccccttgg ctttttaacc actggacttt
     2641 gttctccatc tctgtgatgt cgctgtattt gctcttgctg acgtacccca gacaaatact
     2701 gctgataagc gttatacatg ccagtgaaat caaggctata ttcgatgtaa taagggtttt
     2761 ctttaatttg gctgcttggg ctgtttcttg gctcaagctg ttcaactgct gcaagttcgc
     2821 ttgtagctgt ttgatctgct gttgttgttc ttgcaatagg gctgtcatgc cttgttgctg
     2881 ctgcttcttg gtttgagctg tagtctggtc gatctgctgc aatatctcgt tcgttgcttc
     2941 gctctgtttc tgctgtatat acgttttggt gctgtctata ctgctgctca agctctgtgc
     3001 ttcgctttgc actgtattcc gcatatcttt gaatatctga ctgatttcgc tgtttagctt
     3061 ctcgtctata tcgttctgat gctgcgatag taagttgttg gctcgtttct gtaagtctaa
     3121 aatcttgctc atagattgca cctgtaagcc ttatcgggcg ggctttttcg tcatcattcg
     3181 gatttttaat ggataggctt ttcttggttt ctctggtgac ttcaagacca agatccaata
     3241 gccatttttt catgctctgc cgatctgtaa ttagtccacc ttcaacggct ctcgttgctt
     3301 caatatcaag cgtttccttg atttcattaa cagttttagg caagtttttt gccgttgtaa
     3361 cggcttggcg atagaggggg tcatctgcat catgcagttg atattcatag tttgtaatct
     3421 tcttaaacag atcaatacga ggacgatcag ctttatcata ataaggctgt aaacgttgcc
     3481 ctgtaaggat ttcaacatta gggattagga agttaagctc taatcttcgc tcccctgttt
     3541 cttcgtttaa tttgtcttga tgctcaatcc acaagacacg gtactggctc tgatccatgc
     3601 cagggaataa ggcttgctca aaatcctgca tgattttttg tttatcctgg tcagataaat
     3661 catgctcata aaaacttagg cagccggatg tatatttttt tgcataaggt gacgtatcaa
     3721 ttaatcctga tacttcggaa acatcgccct gtaggacttt tgcataatct cgatcacggt
     3781 ctttaccaag taaataatcc agtggacctt tacttaaacc gttcccatat ctaaagaatt
     3841 gaacaatcat tttttc
//
