LOCUS       NZ_CP061580             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS63 plasmid pAS63-2, complete
            sequence.
ACCESSION   NZ_CP061580
VERSION     NZ_CP061580.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092239
            Assembly: GCF_014719215.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:23:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,131
            CDSs (total)                      :: 4,034
            Genes (coding)                    :: 3,880
            CDSs (with protein)               :: 3,880
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 83 of 154
            Pseudo Genes (incomplete)         :: 72 of 154
            Pseudo Genes (internal stop)      :: 23 of 154
            Pseudo Genes (multiple problems)  :: 20 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061580.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS63"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS63-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5667..6078,1..74)
                     /locus_tag="IC792_RS20490"
                     /old_locus_tag="IC792_20480"
     CDS             join(5667..6078,1..74)
                     /locus_tag="IC792_RS20490"
                     /old_locus_tag="IC792_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            207..875
                     /locus_tag="IC792_RS20495"
                     /old_locus_tag="IC792_20485"
     CDS             207..875
                     /locus_tag="IC792_RS20495"
                     /old_locus_tag="IC792_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(1034..1567)
                     /gene="aadB"
                     /locus_tag="IC792_RS20500"
                     /old_locus_tag="IC792_20490"
     CDS             complement(1034..1567)
                     /gene="aadB"
                     /locus_tag="IC792_RS20500"
                     /old_locus_tag="IC792_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(1658..2344)
                     /locus_tag="IC792_RS20505"
                     /old_locus_tag="IC792_20495"
     CDS             complement(1658..2344)
                     /locus_tag="IC792_RS20505"
                     /old_locus_tag="IC792_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            3347..3754
                     /locus_tag="IC792_RS20510"
                     /old_locus_tag="IC792_20500"
     CDS             3347..3754
                     /locus_tag="IC792_RS20510"
                     /old_locus_tag="IC792_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3744..5558
                     /locus_tag="IC792_RS20515"
                     /old_locus_tag="IC792_20505"
     CDS             3744..5558
                     /locus_tag="IC792_RS20515"
                     /old_locus_tag="IC792_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             complement(1038..1567)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(1035..1579)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          3745..5555
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgaagctaa gagactatta gctaattatt ttgatgtccc tcaagaaaat atttcaatta
       61 caatcaatat gtaagctttt aattgaccaa aaaaagaaaa aagcctttca cgttaatgaa
      121 aggctttttt gaatcttagc gcttgggagg gaagatctta gatccgcagt ctctcaaact
      181 tacaactata tattagggta tatacggtgg aattgtcaaa tgatgcagtg cttaagatag
      241 agcaggcttt atctatacca cgcttatcta agtatgaaaa tttctataaa gataaaggtg
      301 aaccatatga aaaatctgat gtattgatgc tttatgaacg taatttaata atttccaata
      361 agttttttta cttattaaat tattttgaag ttgttctgag aaatgctgtt gttcaggcaa
      421 tagaaatatc ttttaggtgt aatgaaacaa atagttggca tgaaaatgaa gcttttattc
      481 gaagccttag ccgtagaggg aggtatagcc ctaaaagtat gtttgatagt gctaaagaga
      541 agttcccaga ttcgcctagt aaaatgatcc ctgagcttaa atttgtattc tggcaaaaga
      601 tgcttatggc taattatgaa gaacgtattt ggcagggatg ttttaattct atttttccta
      661 atgcaacagt tgaaaacagg caaatttttt acgattggac agatcaatta cgtgaattaa
      721 gaaacaggat tgctcatcat gaaccgatta tttttaaccg taatctagaa aatgatttag
      781 aggttttaac aaaatttatt tactgtcgtt gtattaatac atacgatttt atcgagcaat
      841 ctgcattaga tctaaaagaa tatttagatt tttaagataa gaatttcacc ccaaacaatc
      901 gaaaggtcag actattttgc aaatgttgaa ttaacaaggt tagtagtaaa gcagtaaatc
      961 cgataacggt agcagtgttt cctaacacct gagttaagcc gcgccgcaaa gcggcgtcgg
     1021 cttggacgaa ttgttaggcc gcatatcgcg acctgaaagc ggcacgcaag acctcaacct
     1081 tttccgcccc gagtgaggtg catgcgaacc tgtaggactc tatgtgcttt gtaggccagt
     1141 ccactggtgg tacttcatcg gcatagtaaa agtaatccca gatgatcgcc tcccagctgt
     1201 tacaacggac tggccgcccg gcgatgacac cctcagccgc ctctgggcac gagccctgcg
     1261 gagcctccgc gatttcatac gcttcgtctg cccaccaagc aggttcgcag tcaagtaact
     1321 catccccgat ctccgctaag aatccatagt ccaactcctc cgtgacgcgc ccgccgagca
     1381 tttcaactat tgcctcgagc tcgccgcgcc tctcgccggg aaaagtcaga tcaatatcat
     1441 cgtgcttgcg tgttacacgc cctagccgtg catcgatcgc ccagccccca ccgatccaga
     1501 gcggcagatt tcgctcatct gccgcagcta gaatttggtg tatcaatgcg acctgcgttg
     1561 tgtccatgcg gcctaacata gtaagccctc ccagaatgtc agtaattaga ctattaattg
     1621 aattgttaca tccaatagtg agctcccagt gattgtctta actatccaac ctttcgattg
     1681 gatggggcag agcaccatta ttctgtttga atgcggtgtt agtgtcaact aggttcttta
     1741 tttcttcaag gagtttgaaa gtgctaagta tttttacttt actttgttcc tcaatataaa
     1801 tttttttaag accatttagc agcttgtatt tggatatttt ggataggaga atgaataagg
     1861 ttgcggtatt gattcttccc ccttttagat gaagtaaacg gtttttttgc agtattggaa
     1921 tatttctata ttctgttagt tgatctttta agtcatcagg aattttcatt tcgtaaaaat
     1981 tatatttttt agaacagtct aatatcgagt gatttaggct ttctgtattt gtattaataa
     2041 aaatgcattt ttcaaatgtg caatttttaa agcgacaatc tctaaaaata gcagcataaa
     2101 atacgcactt tttgaagtga acattcgtaa acctattctt atttaagatt actcctgaaa
     2161 aatcagataa aacaattttt atatttttga atgaacatga tcgtaggcgg gatcctctca
     2221 aattaacatt aaaaaatgtt ttattagaga aacgtacttt agaagaaata gatttggcta
     2281 ggaggctatt tgaatactta cgtttttcga tgtgcttgtt atgaaatcta ggaagttttg
     2341 acattaatag tccaaagaat aggagtttca gcttaattct taggtgtaat caaaaaaaat
     2401 aatggacagg tctcttgcga aatctgtcca ttagttttaa attcgatggg tacagttatt
     2461 tgatgcttgc aacatcaatt aacgaaagat cggcagagct tgcaactcat actgatattc
     2521 accttaaagg ctagtaccac tatctatttt aaagaattag aacagtttga caagcctcaa
     2581 tttgaagtcc ttcgttaatt cagcagttta aatcatcaaa taagcaccta acggtcgagc
     2641 tttatctact tctgtgcgcc ttttttaagg aagatatgcg ttgcatgtgg agggaataag
     2701 tgtcactggt gcatcgtagc gatagtcgca aggctaaacc gcttacaggg gatctcacat
     2761 aggcggagcg ttggaagtag ttgtataggt tggttaagtt gcatgttgga gtaatcctcg
     2821 cagagatacc accatacttc gacagggagc gcgtgggaga aggtgcaaaa cctttagtcc
     2881 ttgtctgcat ggtcagcaaa accatgtgcg gatacgaccg atgcgtggct ccgtcagccc
     2941 aattacaggt taatcggcaa tacttaagag gatatttgat ttttttaaat caaagcctct
     3001 tagggaaagt attgccgaaa ctctctcaac gttcaccatc agcccgagca aagcgaggga
     3061 tgtggggacg gtgagaaaga gttgagcgca tttgttttta caagattcat gccaatacct
     3121 actagagttt atataccgtg tcatttgatt agtttttatt gaatttcact cgtccatttt
     3181 taaataagca aggatacaca gctaccccta tttaacttcg ttaaacagtg atagtctgtg
     3241 accttgatca ggggcttcac ccccgatacc cccgaaaagc tttaaacaga aaaatcaaag
     3301 attttccaat ttaaagctta caaaagccaa agcagggtag attgaaatgt caggacagga
     3361 acaagaaaac aaacagagca aagcagttcg agaacgaacc tttaaagtca gactttcaga
     3421 atcagaatat caattgctcg attccaataa tccttatggt tcgattgcaa agttgcttag
     3481 acaagcagca gtagagaaag cgactaaaat tcataattcc aaagcagaag atgaccaaaa
     3541 aattaaagca acaaaggttg ttgaatcttc gtatagcaag acagagcgag cattaatttt
     3601 agagcttggt cggattggca acaatgtgaa tcagattgct aaagcagtga ataccgatat
     3661 tgcaggggta ggtacttttg acaaagtgaa gttacttcat ttgttgattt ccatagatca
     3721 gcagcttcgg gagttacgtc cagatgatcg ttgatttctt tagacatggt tcaggtttat
     3781 caaaaggctg tctcgattat ttgttaggtg aagatcgaga gcgtgaacat gctcaagtgc
     3841 tcaatggtga tgtagaactg acagcacaac tcattgatag cagtcctttt gccaaaaaat
     3901 ataccagtgg ttgtttgtcc ttttatgagc atgatttatc tgaccaggat aaacagaaaa
     3961 tcatgcaaaa ttttgaggaa tgtttattcc cgggactaga taaagaccaa tatcaaattt
     4021 tatgggtaca gcaccaagac aaaattaatc aggacacagg tgagacaagg cttgagctta
     4081 attttgtgat tcctaatgtt gagctttcaa caggcaaacg cctacagcca ttctatgcgc
     4141 cagtcgattt agatcgagtc gatctattta agcagatcac taataccgaa cattcactgt
     4201 atgaccccga tgatcctgag catcgacagc tatttttaaa taaaaagaac ctacccaaag
     4261 atattaagga ttttaaagcg cagttacatc aacgtgttta ccgagcggtt gccaatggtg
     4321 atgttgccga tcggcaagaa ttggtgcaat ggcttgaatc gaatcaaatc aatgtcactc
     4381 gacaagtaaa aaactccata tccattgaaa acccatatga gggcgcaaaa cgtcccatcc
     4441 gtttagaggg cgagatttat gagcaaggct ttagagctac tggcgaatat agacaagaag
     4501 tacaacaacg aattgaagaa tacagaggaa caacttctga gcggtatcga acaaacgtca
     4561 cagactatca gagacagctt gaacataaga gccaatatca ctgcgaccgc tatccaacag
     4621 tcggacgaga aaatagccca gagcattcaa aacaacgccc agacggtcga gaagcagtta
     4681 agcctgtatc aaaccttgct gcaatcgaaa ttgagccatt taacgcaatt aaacgagcaa
     4741 atacagagcc aagaacaaca agcccagaat caagcggtac agaaaaaact taccactttg
     4801 aatatgggac tgatttcagt agcagttatt ttgcttatag caattttctc gcttggtctc
     4861 gtcaccaaaa acaagtacag cgagatgcag acgctaaata ccgtgatcaa cgagaagcaa
     4921 atgagggtcg accatttgaa ccgatcggga ggcagtttga cgatcagcac atgcaagcga
     4981 ccagacagca aagcacagcg cctatgtatt cagatcaaca agaacgcagg cgaatggcag
     5041 aacggctaca tgattccaat ggggtattaa acgatgaccg aattagaaac acaattattg
     5101 agaatcatcg aagaacaaca gcaacaatta atgcaacaac aagtgcaatt gcagaagcaa
     5161 cagcaagctt tagacacaat gcaaaccaaa attatccaag ccttgtcagc acaatcaaag
     5221 acaatcaaga gcgatcagca aagcttacag caagcacaga aatcattagc gacaatgcag
     5281 aagcaatttc acgagctaga acgaagtaca gcaagctata ccgagcagat caatggcaaa
     5341 gtccgagaga tgagccttat cacagccaac aacgaagaaa ttctggagag aatctcaacc
     5401 acacagcatt ctgtctcgtc agaagtagcc aaagcgcagc agaatttgct cgaaacatcc
     5461 gagatattga aaaaaatctc gtccagatga atgagcgtaa acaagagata gaaaaacctc
     5521 gacctaagca agatcgaggt atggattttg ggatgtaatt attttctagt atgttggtgt
     5581 ttatttctta gtattagctt gtactaatgt ttaaatttag ctatattgaa tgtagtatag
     5641 gcttgtttat atattttggt ggtgttatga ataataactg tgtagtagta ctaactgctc
     5701 gtggaaagac tagaatttta aaagaaggtg gtagtcagtc ttggagacta aatgctcata
     5761 atgcaggaaa gtgcgattat ctagtttgtg tgcaaaatag gaaacaatct tggggagaac
     5821 cagaggctga tcatcacact gcatttttag ttggcaaaat atctagtatc agccaatctc
     5881 aagaagaaga tgcagatggt agatggataa taaatatctc cgaatatgct gatatatcta
     5941 ttcccaatat gtgggatggt aatcggaatc cagtttctta tagttcatta gaagagatga
     6001 atatcgattt gaatgagctg aaatttacca aattaaatga agataattct tctaaaacac
     6061 tggataaaat atctattc
//
