LOCUS       NZ_CP061577             3492 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS70 plasmid pAS70-6, complete
            sequence.
ACCESSION   NZ_CP061577
VERSION     NZ_CP061577.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092240
            Assembly: GCF_014718955.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:21:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,298
            CDSs (total)                      :: 4,202
            Genes (coding)                    :: 3,745
            CDSs (with protein)               :: 3,745
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 457
            CDSs (without protein)            :: 457
            Pseudo Genes (ambiguous residues) :: 0 of 457
            Pseudo Genes (frameshifted)       :: 343 of 457
            Pseudo Genes (incomplete)         :: 117 of 457
            Pseudo Genes (internal stop)      :: 33 of 457
            Pseudo Genes (multiple problems)  :: 30 of 457
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061577.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS70"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS70-6"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(2984..3492,1..781)
                     /locus_tag="IC793_RS21220"
                     /old_locus_tag="IC793_21210"
     CDS             join(2984..3492,1..781)
                     /locus_tag="IC793_RS21220"
                     /old_locus_tag="IC793_21210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_307887830.1"
                     /translation="MNAREFLGGRSKLSKMQTDFYNEVKHLGLERGLEGSKAEHTTIKD
                     FYAEIQKPDNEKYLIQKPPLKTITYNDSAIHMLDGKPLKDEKLQKYFGSLFDEQHEYYQ
                     KQSLKAQEALSVKLSEQIIKTEKEAEAHRNAVKQIKKLEKKIDKMLDEFSQIIEFKELF
                     PNDYKEIEQSLKDRIQDHKNQLIRKREEDARFEAQLRAQREQLAKEQERQQAIEFRKTL
                     ESDRKMQIEADRAHLRDKVGECTTEPEKLAYSAIQRKLEGVIREGANYTVLLDNNEPTT
                     NPYAEILRLMGDREVYDFKKKLEIILNQCADSKSKDYDDTFRSWGTRPSYYDNFDSSAK
                     YIVAIQSIIDDQVQEGGVLAYEKANEVKRALSDVLVGKCKNEYDVLLIDEKSKERQRQY
                     NESLELSNKNQLDNSLYFEEEKKKGNDFEM"
                     /name="hypothetical protein"
     gene            1162..1830
                     /locus_tag="IC793_RS21790"
     CDS             1162..1830
                     /locus_tag="IC793_RS21790"
                     /inference="COORDINATES: protein motif:HMM:NF013603.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_307887831.1"
                     /translation="MLNNLNDQNLPSSTNSALASFGGVTAFKKSRLGTIVKLCSTVDSK
                     EKNLALEKAREYRKRNIKLKDRAGEILYIPNQKKQAPVCSCGKCRIDGSAPVGVDYNQK
                     TGMASYTNLQYCGSVWVCPDCSYKISQERKKELAEAMKGCVDKGLHVAMLTLTVPHYLG
                     DDLRALLKKMSKAKHSLWSNRNSREYIADQFPMIGHITATEVKYSDSNGFHPHYHILCF
                     "
                     /name="protein rep"
     gene            1821..2489
                     /locus_tag="IC793_RS21795"
     CDS             1821..2489
                     /locus_tag="IC793_RS21795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_307887828.1"
                     /translation="MFLDKQYQKEDLEIIENELYEFWAEKCVKAGLGKPNRRNGIDLKM
                     GSNNADTLADYIAKWGIAEEMTQAHLKIGKNNLQSLTMWEVLELAQMEASTTDKYSHIF
                     KTYAVAFKGRKQLHWSKGLKKLLECPVKDDEEIANTNTEEDPTEVVHALFLSPQDFWAI
                     CYHKKRAEFLELVESDFAKNGIQTDLKSVRDFLIRLKNARRFQGVKGKSPPLRADNLKT
                     "
                     /name="hypothetical protein"
     gene            2580..2987
                     /gene="mobV"
                     /locus_tag="IC793_RS21800"
     CDS             2580..2987
                     /gene="mobV"
                     /locus_tag="IC793_RS21800"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_307887829.1"
                     /translation="MSYAILRTEKLKTMGNIAASLSHNYRNRPTPNADPYRTVNNEHDL
                     KTAGQVMDGIKNRLPEKTRSNAVLCVEYLITMSPDWSGLGTEKEADFFKRSLEWLKQRH
                     GAENVISTSIHRDETTPHLVAYVVPIDLEGN"
                     /name="mobV"
ORIGIN
        1 agaacaaagt ttaaaagatc gaatacaaga tcataaaaat caattgatta gaaaaaggga
       61 agaggatgca cgatttgaag cgcaattaag ggcgcaacgt gaacagttag caaaagagca
      121 agagaggcaa caggcaatag aatttaggaa gacactagaa agtgatcgta aaatgcaaat
      181 agaggctgat agagcgcatt tacgagataa agttggtgaa tgtactactg aacctgaaaa
      241 actggcttat agcgcaatac agcgcaaatt agagggtgtt attagagagg gggctaacta
      301 cactgttttg cttgataata atgagcctac aactaaccca tatgccgaga tacttaggct
      361 aatgggtgat agagaagtat atgattttaa aaagaaatta gaaattatat taaatcaatg
      421 tgcagattct aagagtaaag attatgacga tacttttaga agttggggaa caagaccaag
      481 ttactatgat aattttgatt catctgcaaa atatattgtt gcaattcaaa gcattataga
      541 tgatcaagtg caagaggggg gtgtattggc ttatgaaaaa gcgaatgaag taaaacgtgc
      601 tttaagtgat gtattggttg gtaagtgtaa aaatgaatat gacgtacttt tgattgatga
      661 gaagtcgaaa gaaaggcagc gtcaatacaa tgaatctctt gaattatcta ataaaaatca
      721 attagataat agtttgtatt ttgaagagga aaagaaaaag ggcaatgatt ttgagatgta
      781 gactttagcg agtgtctacg agcgtcacag tagtagtaaa attcatgatg tttttctctc
      841 tgattaccta ctcccttttg atcgcaaacg ggagtaacag agcatcccaa cgggtgcgaa
      901 caacttagaa aacaatggct tagatattta actagagtaa atatttagtg taatttttac
      961 tttaaaatca gatatttgaa taacagaaat atttaagtta tattactttt agttttttac
     1021 agaccaaaaa aaagccccat atcttggcgg atcaggcttt tgattggcta ctttcagcgg
     1081 cttgaggtgc tgatttcaca atgtatacgg acagtataaa ggtgatttcc ctctccatca
     1141 agcctgatta tgggcttgag catgctaaac aatctaaacg atcaaaacct accttcctct
     1201 actaacagcg ctctagcgtc atttggtggc gttactgcat tcaaaaaaag tcgccttggg
     1261 acaatcgtca aattatgttc caccgttgat tctaaagaga aaaatttagc tcttgaaaag
     1321 gctcgtgaat atagaaaacg aaatattaag ctaaaagata gagcagggga gattctctat
     1381 atcccaaacc aaaagaaaca agctccagta tgtagctgtg ggaaatgtcg tattgatggc
     1441 agcgcccctg taggtgtgga ttacaaccaa aaaacaggaa tggctagtta tacaaatttg
     1501 cagtattgcg gttctgtttg ggtgtgtcct gactgtagtt ataaaattag ccaggaacgg
     1561 aaaaaagaat tagccgaagc gatgaaaggc tgtgttgata agggtttgca tgtggcaatg
     1621 cttacattga ctgtgcctca ctatcttggt gatgacctcc gagcattgct taaaaaaatg
     1681 agtaaggcga agcattcttt atggtcgaat agaaatagcc gtgaatacat tgcagaccaa
     1741 ttcccaatga ttgggcatat cacagctaca gaagtgaaat atagtgatag caatggtttc
     1801 cacccccact accatatcct gtgtttttag ataagcagta tcaaaaagaa gatttagaga
     1861 ttatcgaaaa tgaactttat gagttctggg ctgagaagtg tgtcaaagct ggacttggta
     1921 agcctaatcg taggaatggt atagatttaa aaatgggttc taacaatgct gatacccttg
     1981 ctgattatat tgctaagtgg ggaattgcag aggaaatgac tcaagcacat ttgaagattg
     2041 gtaaaaataa tttgcagtct ttaacgatgt gggaagtcct tgagcttgcc caaatggaag
     2101 caagtacaac agataaatat tcacatatat ttaaaaccta tgccgttgct ttcaaaggaa
     2161 gaaagcaact tcattggtca aagggtttaa agaaattatt agaatgtccg gttaaggatg
     2221 acgaggaaat tgcaaataca aatactgaag aagatccgac tgaagttgtt catgctcttt
     2281 ttcttagtcc tcaagatttt tgggcaatct gttatcacaa aaaacgtgcc gagtttcttg
     2341 aactcgttga atccgatttt gctaaaaatg ggattcagac cgatctaaaa tccgtaaggg
     2401 attttttgat caggcttaaa aacgcaaggc gttttcaggg ggtcaagggg aaatctcccc
     2461 ctctgcgagc ggacaatttg aaaacgtagt tttaaaattg tgtagtgagt acacattccg
     2521 cttcgctaat gtttttccct gtggcacact gggggaaaac tagcgatagg atgtgtttta
     2581 tgagttatgc aattctcaga acagaaaaac tgaaaacgat gggcaatatt gctgcatctt
     2641 tgagccataa ctatcgcaat agacctactc ctaatgctga cccataccgc actgttaata
     2701 atgaacatga tttaaaaaca gcaggacagg tgatggatgg gattaaaaat cgacttcctg
     2761 aaaaaacacg ttcaaacgct gtcctatgtg ttgaatattt aataacgatg tctccagatt
     2821 ggtcgggatt ggggacagaa aaagaagcgg atttttttaa acggtctctg gaatggttaa
     2881 aacaaaggca tggtgctgaa aatgttatta gtacctcgat ccaccgtgat gaaactaccc
     2941 cgcaccttgt tgcatacgtt gttcctattg atttagaggg aaattgaacg ctagggaatt
     3001 tttaggcggt aggtcaaaat tatcaaaaat gcagactgat ttttataatg aggttaagca
     3061 tttaggactt gaaagggggt tagaggggag caaggcagaa catacgacaa ttaaagattt
     3121 ttatgcagaa attcaaaagc ctgataatga aaaatattta attcaaaaac cacctttaaa
     3181 aacaattact tataatgatt ctgcaattca tatgctagat ggaaaacctt taaaagatga
     3241 gaagcttcaa aaatattttg gaagtctttt tgatgagcag catgaatact atcaaaagca
     3301 gtctttaaaa gcccaagaag ccctatctgt aaagctttca gagcaaatta ttaagacaga
     3361 gaaagaagca gaagcccata gaaatgcagt aaaacagata aaaaaattag aaaaaaagat
     3421 tgataaaatg cttgatgaat tttctcaaat tattgagttt aaagaacttt ttccaaatga
     3481 ctacaaggaa at
//
