LOCUS       NZ_CP061576             4391 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS70 plasmid pAS70-5, complete
            sequence.
ACCESSION   NZ_CP061576
VERSION     NZ_CP061576.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092240
            Assembly: GCF_014718955.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:21:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,298
            CDSs (total)                      :: 4,202
            Genes (coding)                    :: 3,745
            CDSs (with protein)               :: 3,745
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 457
            CDSs (without protein)            :: 457
            Pseudo Genes (ambiguous residues) :: 0 of 457
            Pseudo Genes (frameshifted)       :: 343 of 457
            Pseudo Genes (incomplete)         :: 117 of 457
            Pseudo Genes (internal stop)      :: 33 of 457
            Pseudo Genes (multiple problems)  :: 30 of 457
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061576.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS70"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS70-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(3882..4391,1..420))
                     /locus_tag="IC793_RS21185"
                     /old_locus_tag="IC793_21175"
     CDS             complement(join(3882..4391,1..420))
                     /locus_tag="IC793_RS21185"
                     /old_locus_tag="IC793_21175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_151686088.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQAETDLEHSAY
                     GNMPKWAEHNPINFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGNDQPHVHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDN
                     TGKSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKEPEKKLLPSQA
                     KDPEIREALQQSRTAYKELERLDLGDPKNDLKDLKDSPISDKEIKQGIESFKADFDSFK
                     QLSLQQYKEQQKLEREQQKTMSYKGMSR"
                     /name="MobA/MobL family protein"
     gene            complement(420..575)
                     /locus_tag="IC793_RS21190"
                     /old_locus_tag="IC793_21180"
     CDS             complement(420..575)
                     /locus_tag="IC793_RS21190"
                     /old_locus_tag="IC793_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_018680092.1"
                     /translation="MQLKQVLGKIKDIVFGVIVAPLLIIIMMWASILDQRAFKRELDRK
                     KGQDKT"
                     /name="hypothetical protein"
     gene            854..1264
                     /locus_tag="IC793_RS21195"
                     /old_locus_tag="IC793_21185"
     CDS             854..1264
                     /locus_tag="IC793_RS21195"
                     /old_locus_tag="IC793_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_151686090.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKLNA
                     LLKAEKAIDRANKQKIAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     VLLKTAEIDPNDMKWQIWKDLGQETLNKRKIT"
                     /name="conjugal transfer protein TraD"
     gene            1314..2030
                     /locus_tag="IC793_RS21200"
                     /old_locus_tag="IC793_21190"
     CDS             1314..2030
                     /locus_tag="IC793_RS21200"
                     /old_locus_tag="IC793_21190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151686092.1"
                     /translation="MGLFPCEFGFPFNKDSFKSIFFTNNDYSSRSVSFELEFKNSFNFD
                     AVLFTENNLEDNSHYEYAKHTDYEIDVNLAPYAEFAKLSIHISDKESGKVDFDVLNNTF
                     WISIYLSEKDFDALIPTIKDKDIYIDFNILCTKSDDDVEDTIKKLIVDDVKKQVLVLNK
                     FVKSVKIGKLTLTAKNPKLEKIESDENQLENNKEDLNKELISKASTTNQLLTQLLKKQE
                     MIFYIIIALLILIFFK"
                     /name="hypothetical protein"
     gene            complement(2843..3058)
                     /locus_tag="IC793_RS21205"
                     /old_locus_tag="IC793_21195"
     CDS             complement(2843..3058)
                     /locus_tag="IC793_RS21205"
                     /old_locus_tag="IC793_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004847384.1"
                     /translation="MQNELERYKNFDPTNAPLVKPSFVKKLQERHAIAQAQAFDADVVA
                     WLSSQDNETKQHINDMVRHIMALKRA"
                     /name="hypothetical protein"
     gene            complement(3048..3344)
                     /locus_tag="IC793_RS21210"
                     /old_locus_tag="IC793_21200"
     CDS             complement(3048..3344)
                     /locus_tag="IC793_RS21210"
                     /old_locus_tag="IC793_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_004846796.1"
                     /translation="MPLIEYYGLLFEWHDQKMDLVYRGREITFEEVCSIFLDPALMSFE
                     DVGHYDEQRLISVGLSNRGRLLTVVWVERGDVARIITAFEPSHHQKRRYSNAK"
                     /name="BrnT family toxin"
     gene            complement(3385..3885)
                     /locus_tag="IC793_RS21215"
                     /old_locus_tag="IC793_21205"
     CDS             complement(3385..3885)
                     /locus_tag="IC793_RS21215"
                     /old_locus_tag="IC793_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151686086.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVTQAVKELQITEERIQKVIAGQTH
                     STVSKSLNTGLQEGTAELIKTAKALGHLKNSIESTSDQFSWKIITIILGVFAILMLGII
                     WLFSVYIKPLENIPQIESLRQQGIQFDIGSCTVGEESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     Typing          complement(2..420)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.714"
                     /coverage="100.0"
                     /evalue="2.05e-83"
ORIGIN
        1 tcgctcgatt ccatcttgca gacgttcatt aaacatgagg tgaacatgag gctgatcatt
       61 gccgtccatt gctttagggt tatgaatcgc aaactgatac ggatattttg agccaatttc
      121 agactgaatg aagtcctcaa ccagttctaa gcgttgctcg gcattcatct ctctaggcaa
      181 agctatttca tactctcggt aggtactgcc gtttttacgc tcgtaaaggt cggctgcctg
      241 ccaaaagtta atcggattat gttcagccca tttcggcata tttccatagg cactatgttc
      301 aaggtcggtt tctgcctgct cctgtttcag cttcttttct tcatccctat caatatattc
      361 ggcatgcggt gctgctttgc ctgctttacc aactttgaca cttaaacgag caatcgccat
      421 taggttttat cctgtccctt ttttctatcc agttctctct taaatgctct ttgatcgagt
      481 attgaagccc acatcataat gatgataagt agcggtgcta cgatcacgcc aaaaacaata
      541 tctttgatct ttcccaatac ctgttttagt tgcatgcggt tgctcctagc tttggtactt
      601 ttttttcgtt caattttcaa ttgaacgaaa aaaagaagtt tcagggtttt agggatgaag
      661 tccctaacgt gcttaggttt ctcaatgaaa ttgagaaacg tctaagcgcg ccatcaaatt
      721 tttgatggaa gtacaggcga caatctctag aacgacttta acaaaattac gttactcgaa
      781 cgtaaatatt ttgttatcgt tctttgagta ttgagcctgt gcttttgcgg tatctgaatt
      841 aatggaattg aatatgaata atgattggct gtctgaaaaa atcacctata tttccgcttt
      901 gaagaatcct agtgatgctc aaaagctttt attagaatta gctaaaatcc aataccgtac
      961 acccgaccaa gagaaaaaac tgaatgcctt gctcaaggct gaaaaagcca tagatcgagc
     1021 gaataagcag aagatagcag ttagaaaatt actgaatgca gaaaaagaag ctgaacgtaa
     1081 agctcgtaca cgtcatctca tccaattagg cgcacttttt gaaatcgcta acctagatca
     1141 gcgtgaccca gccgaattac ttggagttct gcttaaaact gctgaaatcg acccaaatga
     1201 tatgaaatgg cagatttgga aggatttagg acaagaaacc ttaaataaac gcaaaattac
     1261 ttaattttat tttgattttt accatacatc tttaactaat attggaggtt aatttgggac
     1321 tttttccatg tgaatttggt tttcctttta ataaggattc attcaaatct atatttttta
     1381 ctaataatga ttattcaagt agatcagtat cttttgaatt agagtttaaa aattccttta
     1441 atttcgatgc agttcttttt actgaaaata atctagagga caatagtcat tatgagtatg
     1501 ctaaacatac agattatgaa atagacgtca atctagctcc ttatgctgaa ttcgctaaat
     1561 taagcattca tatttctgat aaagaatcag gaaaagttga ttttgatgtt ttaaataata
     1621 ctttttggat ttcaatttat ttaagtgaaa aagattttga tgctctaatc ccaacaatta
     1681 aagataaaga tatttatatt gatttcaata ttttatgtac taaatctgat gatgatgttg
     1741 aagatactat aaaaaaacta atcgttgatg atgtcaaaaa acaagtttta gtattaaata
     1801 aatttgtcaa atctgtaaaa ataggaaaat taacattaac tgctaaaaat ccaaagttag
     1861 aaaaaattga atctgatgaa aatcagcttg aaaataataa agaagattta aataaagagt
     1921 taataagtaa agcttcaact acaaatcaac tcttaactca gttattaaaa aaacaagaaa
     1981 tgatctttta cataataatt gcattattaa tacttatatt ttttaaatag cctaagcata
     2041 aaataaggaa tagctcctat ccctcactcc aaaagaaaaa gctcgaactc tctcgcccgc
     2101 ccccaaagcg tcgcacttcg tgctttggct ttggaaacgg ttgagagagt ttcggcaata
     2161 ctctccctaa aggcggtcat ttaaaaaaat gacgcatcct ttaagtattg ccgatttacc
     2221 gcatattctt ttggcttctg gagccacgta tatcggtcgt atccgcacat agctttgcaa
     2281 actatgcaga caaggactaa aggtcttgca cctctcccca cacgctccct gtcgaagtat
     2341 ggcggtatct ctgcgagggt ttccccaaca tacaacttaa ccaacctata caactactct
     2401 taacgctccg cttttgtgag attcccctgt agcggtttag cctttcgact atcgctacgt
     2461 tgcaccagtg acacttattc cctccacatg caacgcatgc caatccgtaa aacggacacg
     2521 caggagtagg caaagctcga tctaggatgc ttatttgatg atttaaaaca cggattaacg
     2581 aagacttatc attttgggtc ttgaactaaa cactgtttat tgtgaaaata gagttcaggt
     2641 acaagcctta gtgtgaaatc tgcacgagtt ggtagctctg ctgatcttcg ttattcgatg
     2701 gttcgaagca tcaaataact gcgtaccgct gaatataaag cgaaagccca ttgagttgca
     2761 agattcaatg ggctttttct ttgatcaatc agacattaag acaaaaaagg gaggtcatat
     2821 ctaatcatga ctaaatccct aatcaagcac gtttcaatgc cataatatgg cgaaccatat
     2881 cgttaatatg ttgttttgtt tcattatctt gtgaacttaa ccatgcaact acatcagcat
     2941 caaaagcctg tgcttgagct atggcatggc gttcctgtaa tttcttcaca aagctaggtt
     3001 taaccaatgg cgcattggta gggtcaaaat ttttatagcg ttctagttca ttttgcattg
     3061 ctgtacctcc gcttttggtg atgtgatggt tcaaatgcag tgattattct cgcaacatct
     3121 ccacgttcaa cccaaaccac tgttaatagt ctaccacgat tgcttaaacc gactgatatt
     3181 agtcgttgtt catcatagtg tcctacatcc tcaaaagaca tcaatgcagg atcaaggaaa
     3241 atactacaga cctcctcgaa cgtaatttca cgccctcgat agactaaatc cattttttgg
     3301 tcatgccatt caaataacaa gccgtaatat tcaattaatg gcatgattac ctcttttcct
     3361 ctcgtgaaat aaaaaatatg cctattattt tgggtctatg acacaaaaat ctccattttt
     3421 tccatatccg cactgctttt tcatcactcg aacacacggt ttagattcct ctccaacagt
     3481 acagcttcct atatcaaact ggattccctg ctgtcttaag ctctcgatct gcgggatatt
     3541 ttctaaaggt ttgatataaa cactgaataa ccaaatgatg ccaagcatca gaatggcgaa
     3601 aacgcctaaa ataatggtga tgattttcca tgaaaattga tcactggttg attcaatcga
     3661 gttttttaaa tgtccaagtg ctttggcggt cttaataagc tccgctgtac cctcctgtaa
     3721 gcccgtattt agcgattttg atacggtact atgggtttgc cctgctatga ccttttgaat
     3781 gcgttcttcg gtgatttgaa gctctttaac agcctgagtg actaggttct gctgttgttc
     3841 tgcaactgcc atcagtgcat ataacttttt ttcatcaggt gtcatcggct catcccctta
     3901 taactcatgg ttttctgttg ctctcgttct aatttctgct gttctttgta ctgctgtaag
     3961 gaaagttgtt tgaagctgtc aaaatcagcc ttaaagctct caatgccctg tttaatttct
     4021 ttgtctgaaa ttgggctgtc tttgaggtct ttcaggtcat ttttagggtc gcctaagtct
     4081 agtcgctcaa gctctttata ggctgtacgt gactgttgca aggcttctct gatttcaggg
     4141 tctttagctt ggcttggtaa tagtttcttt tcgggttctt tctctatgcc ttgctctttg
     4201 tagcttcgca tatcaatacg gctatcaatt tggtgttttt ctaaatggct gttgcataaa
     4261 tctgcccatc tttggcgtag gtctttaata tcagtttttc gttcctggta actcttgcct
     4321 gtattgtctt ttttagctcc gccacgttca ggatttttgc tgttatagcg tttgaaatat
     4381 tgctctggat c
//
