LOCUS       NZ_CP061574             8694 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS70 plasmid pAS70-3, complete
            sequence.
ACCESSION   NZ_CP061574
VERSION     NZ_CP061574.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092240
            Assembly: GCF_014718955.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:21:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,298
            CDSs (total)                      :: 4,202
            Genes (coding)                    :: 3,745
            CDSs (with protein)               :: 3,745
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 457
            CDSs (without protein)            :: 457
            Pseudo Genes (ambiguous residues) :: 0 of 457
            Pseudo Genes (frameshifted)       :: 343 of 457
            Pseudo Genes (incomplete)         :: 117 of 457
            Pseudo Genes (internal stop)      :: 33 of 457
            Pseudo Genes (multiple problems)  :: 30 of 457
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS70"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS70-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            9..911
                     /locus_tag="IC793_RS21095"
                     /old_locus_tag="IC793_21085"
     CDS             9..911
                     /locus_tag="IC793_RS21095"
                     /old_locus_tag="IC793_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237875.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_191102593.1"
                     /translation="MNLTHRQLMMFRAIMLHGNLGRAADACASSQPTLSRELGRLEQLL
                     GFNLFDRVRGRLRPTVRALALMQEVERSFIGLEQIAARASELRTQATGRLRIACLPALA
                     HALVPRALVYLSHTHPELGVSVHPLESPWLEQAMSEQRFDLGLSETQKAPVGVTLQPLL
                     KVNEVAVLPRDHSLCRKARLQPRDFTGERFISLAVGDPYRTAIDAMFEDAKVHRVTLLE
                     TTSSVAVCAMVRQGLGVAIVNPFTAMELSGPDLIVRSLTVPISYQVNMLFPEIAAPHPL
                     QGVLADTVRKASADLSHLD"
                     /name="LysR family transcriptional regulator"
     gene            complement(1205..1720)
                     /locus_tag="IC793_RS21100"
                     /old_locus_tag="IC793_21090"
     CDS             complement(1205..1720)
                     /locus_tag="IC793_RS21100"
                     /old_locus_tag="IC793_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000855181.1"
                     /translation="MKTLTVLELSKLYNINRQTIYNNIKKGILSKNSENKIDLAEAIRV
                     FGEPVKKQDVKEFVKVDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKAPKMEDPTPVVPPQETIEVPVPEQATEVKTEIQTQNKRIPVPEHVEQEPEKRGFW
                     SRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1799..2728)
                     /gene="repM"
                     /locus_tag="IC793_RS21105"
                     /old_locus_tag="IC793_21095"
     CDS             complement(1799..2728)
                     /gene="repM"
                     /locus_tag="IC793_RS21105"
                     /old_locus_tag="IC793_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_016659737.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARQTGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDVNPKTGKDRSFHCRWVDKIG
                     YEAQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PVFDLSIFRQQLGVEPHQYKTMSNFKTYVLDFALNQINELTDIIAKYEQHKKGRTISGF
                     SFTFRQKKDTSKKVITSEKPSDYFSKITDPQRHLFANKLSRLPEMNTYSQGTESFEQFA
                     VRIAEMLKDAQKFEELFPYLQKVGFAPA"
                     /name="repM"
     gene            3586..4266
                     /locus_tag="IC793_RS21110"
                     /old_locus_tag="IC793_21100"
     CDS             3586..4266
                     /locus_tag="IC793_RS21110"
                     /old_locus_tag="IC793_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_307887822.1"
                     /translation="MAYLFCRTSDAFLFDEKTQKVLNKKDKQDLVKINRRIFEICPSAE
                     FFNYIGDVDPILSGKYELVRLTKPKKSIKELQAKNWTNEKHATDWTWRLTDKAYKEQLE
                     QGKRVVLRFQSLIEKNASLDEKKAYFERHFRALEGYLGYRGVRQQIGNLYHLEKRLFND
                     KYNHPWFDHGARTLKLSYIKKIKNMIANNTPYQEAESCYVTVLMEAFITKHEKQREKSN
                     KIEV"
                     /name="hypothetical protein"
     gene            4585..4849
                     /locus_tag="IC793_RS21115"
                     /old_locus_tag="IC793_21105"
                     /pseudo=""
     CDS             4585..4849
                     /locus_tag="IC793_RS21115"
                     /old_locus_tag="IC793_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676203.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5199..5414
                     /locus_tag="IC793_RS21120"
                     /old_locus_tag="IC793_21110"
     CDS             5199..5414
                     /locus_tag="IC793_RS21120"
                     /old_locus_tag="IC793_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
     gene            5512..5739
                     /locus_tag="IC793_RS21125"
                     /old_locus_tag="IC793_21115"
     CDS             5512..5739
                     /locus_tag="IC793_RS21125"
                     /old_locus_tag="IC793_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            5806..6003
                     /locus_tag="IC793_RS21130"
                     /old_locus_tag="IC793_21120"
     CDS             5806..6003
                     /locus_tag="IC793_RS21130"
                     /old_locus_tag="IC793_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            6190..6510
                     /locus_tag="IC793_RS21135"
                     /old_locus_tag="IC793_21125"
     CDS             6190..6510
                     /locus_tag="IC793_RS21135"
                     /old_locus_tag="IC793_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6503..6775
                     /gene="nadS"
                     /locus_tag="IC793_RS21140"
                     /old_locus_tag="IC793_21130"
     CDS             6503..6775
                     /gene="nadS"
                     /locus_tag="IC793_RS21140"
                     /old_locus_tag="IC793_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(7352..8602)
                     /gene="lysA"
                     /locus_tag="IC793_RS21145"
                     /old_locus_tag="IC793_21135"
     CDS             complement(7352..8602)
                     /gene="lysA"
                     /locus_tag="IC793_RS21145"
                     /old_locus_tag="IC793_21135"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068002.1"
                     /GO_function="GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_191102592.1"
                     /translation="MNPFTPTTIAPLAQQFSTPLWIYDADTITRQIAALQLFDVVRFAQ
                     KANSNTHILRLMKRQGVVVDSVSLGEIERALASGFTPGLHNGHADIVFTADLLDRATLA
                     RVVELNIPVNCGSMDMLDQLGAVNPGHPVWLRINPGFGHGHSKKTNTGGEHSKHGIWHG
                     DLVRACEKIKSNSLALHGLHMHIGSGVDYTHLQEVCGAMLKLVKVVNAQGLDLHAISAG
                     GGLSVPYQTGEPTIDTVHYFSVWNAARDAVEQLLEHPVKLEIEPGRYLMAESGVLVTEV
                     RATKQQGTNHFVMVDAGFNDLVRPALYGAYHGMELIHSDGTPVNGLQLDTVVAGPLCES
                     GDVFTQGDGGVVLHRSLPQAQVGDLLVLHDTGAYGASMSSNYNARPLIAEVLMENGEPR
                     LIRRRQTVAELLALEAV"
                     /name="lysA"
ORIGIN
        1 atattgatat gaatttaact catcgccagc tgatgatgtt tcgcgccatc atgctgcacg
       61 gcaacctggg gcgtgcagcc gacgcgtgtg cgtcaagcca gcctactttg agtcgcgagc
      121 tggggcgttt ggagcagtta ctggggttta accttttcga ccgggtgcgt ggccgattaa
      181 gacctactgt gcgggcactc gcgctgatgc aggaagtaga acgctcgttt ataggcttgg
      241 aacagatcgc agcgcgcgcc agtgaattgc gtacgcaagc gacaggtcgc ttgcgcattg
      301 cgtgtttacc ggcattggcc catgccttgg taccgcgcgc actggtatat ctctcacata
      361 cacatcctga actaggagtc agcgtacatc ccttagagtc gccttggttg gaacaagcca
      421 tgagtgaaca acgttttgac ctggggttga gtgaaacaca aaaggcacct gttggagtta
      481 ccttgcagcc cttactgaaa gttaatgaag tggctgtttt gccccgtgat cacagcctgt
      541 gccgcaaggc acgactgcaa ccacgcgact ttacaggcga gcgcttcatc agcctcgcgg
      601 ttggcgaccc gtatcgaacc gccattgatg cgatgttcga ggacgctaaa gtacatcgcg
      661 taaccttgct tgaaaccacg agttcagtgg ctgtctgcgc catggtgcgt caaggccttg
      721 gcgttgccat cgtgaatccg tttaccgcaa tggaactaag cggaccggac ttaatagtac
      781 ggtcgcttac tgttcccatc agttaccagg tgaatatgct ttttccggaa atagcagctc
      841 cgcaccccct gcagggcgtt ctcgcagaca ccgttcgcaa agccagtgct gacttgagcc
      901 accttgacta gtgtttttat tagaactaga aactagaata tttgttacat aggctcaatt
      961 aatcagatga catattctct catttttata cccagaaaat ttcttttaat ctaaagattt
     1021 tctaaaatta acataataca ccttatacga aatgtgttat aattatcttt agaaatcaat
     1081 gctgtagttt ctagttaata gctcatcttc cccaaggtgg gcttttttta tgatttttca
     1141 cgttccacgc ttaataagtg agcaatgttc cacgacttta ttcatattag ttaaataatc
     1201 gtggctaatc atagggtcta aaaaaacggc tccaaaagcc ccttttctcc ggttcctgtt
     1261 cgacatgctc cggcacgggg atccgcttat tctgcgtttg gatctcagtt ttaacctcag
     1321 ttgcttgttc aggtactggg acttcaatcg tctcttgagg tggtacaact ggtgttggat
     1381 cttccatttt tggtgcttta ggagcttcta gtaggcgttg catcgtttcg atctgatttt
     1441 ggtagaaaga ctcacgatct ttagcatcat caagctgatt tttcagcatg tctatttgtt
     1501 gtctaagcag taaaacttct gccgaatttg gactgtctac ttttacaaat tcttttacat
     1561 cctgtttttt gactggttct ccaaaaacac ggatagcttc tgctagatca atcttattct
     1621 cagaattttt acttaaaatt cctttcttta tgttgttgta aatcgtttgt ctattaatgt
     1681 tgtaaagctt ggaaagttca agaactgtaa gggttttcat tcagtaaacc tatgcctaaa
     1741 cttgtaaaaa gttactgtct attagactgt ctaatagaca aatgatcaag tttaaatttt
     1801 aggcaggagc aaagccaact ttttgaagat aagggaataa ttcttcgaat ttttgggcat
     1861 ctttaagcat ttctgcaatg cgcacagcaa attgttcaaa gctttctgta ccttgagaat
     1921 atgtattcat ttcaggaagt ctagaaagct tattagcaaa taggtgacgc tgtggatcgg
     1981 ttattttcga gaaataatct gagggttttt cacttgtgat cactttttta gaagtatctt
     2041 ttttctgtct aaaagtgaac gaaaatcctg aaatagtacg tcctttctta tgttgttcat
     2101 acttagcaat gatatctgtt agttcattta tttgattcaa tgcaaagtct aaaacatagg
     2161 ttttaaagtt actcatagtt ttgtattgat gaggttcaac gcctaattgt tgtctaaaga
     2221 tagacagatc aaaaacggga gtttttcctg ctgacctcca ttggatcagt aattcataga
     2281 gtcgaatagc ataagaacta gttaggcgtg agacctgttc taactcatat ttagtaaaat
     2341 tctcttctag tctggttata agaggaacga tatcttgagt gaatcttaaa aaaacaattc
     2401 ccgactgagc ctcataacca attttatcaa cccaacggca atggaaacta cgatccttac
     2461 ctgttttagg attaacatca tggtatgtga cgtatctatc gaagaggctt tttgatgcat
     2521 ctctaaggac tgtataggca gtatgttttt caacattgaa cgtatttata tagctactgg
     2581 catgtacctc taataagcta ttttctgtta tcccatgacc tgtttgtctt gcctctgcta
     2641 tagctagaag aattaaacgc tgctcaacag tatctaacgt atagctagct tgaattaagg
     2701 cattatcctt aacaattaat tcactcattt ctaataataa atttataatt aaactaggtt
     2761 gtacaataga ctatttaaat aaacgtgtct attaaaatta ttaaggtcga taaacctagc
     2821 tttaaggtcg ataaacctag ctttaaaggt cgataaacct agtttaatag gtcgataaac
     2881 ctagtttaat aggtcgataa acctagttta ataggagcta taacgctttc tacacaaggc
     2941 tttcagctta cctaaaagca tttaaaaact ttaaaataat taaaagccaa gggaatgaaa
     3001 aattgccctt ggttttcgct tcgtttcact cgctcaaact ctattgaatc tcgctttcgc
     3061 tcgattcgtt ggggcaattt tttgcaaata ctttctaaaa ttatgtaaaa agcaaaagcg
     3121 gctatgactt cgcttcgctc agaagttttt ttgctcgctt cgctcggtca agactctgtt
     3181 ttgtacatgt aatctaagtt ttttctagaa tatgaaatag taaaaacggt ttttcaggga
     3241 gtctaggcgc aactttttag tgttcgctcg tagacactcg ctctgatata acgattagag
     3301 gcaaaaaata gggggtagac gcgttttttc actgattatc aagattacct atatcaaata
     3361 gagaaaatgg cttaaaacgc aaatcagcgc aaaatggaaa gcagtttata gtcgtactca
     3421 ggactttgat ctcagttatt tatgtgtgat gagggacaca cagattttta atttcgttca
     3481 gcagaatcgg ctgcgtcagg attttttaag tgaggtcccc actaaccgtt tataaaaagt
     3541 cggatcaaag actttgattt cacttgtttt ctcaaggttc ttggaatggc ttacctgttt
     3601 tgtcgaacca gtgatgcttt cttatttgat gaaaaaactc aaaaggtttt gaataaaaaa
     3661 gacaaacaag atttagtgaa gattaaccgt agaatctttg agatttgtcc aagtgcagaa
     3721 ttttttaact atatcggcga tgttgatcca atcctctctg gtaaatatga attggtacgt
     3781 ctgaccaagc cgaaaaaaag tattaaagag cttcaagcca agaattggac caacgaaaaa
     3841 cacgctacag actggacctg gcgtctgact gataaagcct ataaggaaca actagaacaa
     3901 gggaaaaggg tcgttttacg gtttcagagc ctgattgaaa aaaatgccag tctggatgag
     3961 aagaaagcct attttgaacg ccatttccgg gccttggagg gctatttagg ctaccgtggg
     4021 gtacgtcagc agattgggaa tttgtatcat ctggaaaaac gcttattcaa cgataagtat
     4081 aatcatccct ggtttgatca cggagccagg acattaaagc tttcatatat taaaaagatt
     4141 aaaaatatga ttgccaacaa tacaccgtat caagaagctg aatcttgtta tgtgactgta
     4201 ttaatggaag ctttcattac aaaacacgaa aaacagaggg agaaatccaa taaaattgag
     4261 gtataggatg ttttttcaat cacacataat acctacctag tgaagcaatt taggtaggta
     4321 ttatgtgtga tactaagctt taaatttctt acataatata agtatttgat tttaaatatt
     4381 attgtattta tgtggggaaa ataaaactta aacattaatt ttttaagtta ttgaaattaa
     4441 atacaaaatc aagaacacac atcatagcta ccttgaagca gaaaagaagc tactatgtgt
     4501 gttgcatact acctcacatg cgaaatgtct ttagctaccc gacttagaat atgatttagg
     4561 ttggctttac tcaggattac gtcaatgtca gcagtaaatc taattaatga aaatgacgat
     4621 gagagagaaa ttgcttctca ggcagcttgt gcacttctga gaaagcttta ttaccgcagc
     4681 tcaaagtggc ccagttatgt atgttgaaaa cgatactgtt ttacttaagg aactcagcgc
     4741 tcccccagtt gtgattaaac acctatctgg gcgtaatcct gaactttctc aacgagtagc
     4801 gagtcgtgga acttttaaaa ttaaaaaacg taaagtaagc caggattgaa gctaactggg
     4861 acttacaatt caaatttcgc ataagaggtt ttatgttaaa aaataaatta ttaaaatata
     4921 aatatgttgt tgaattaaat taaattttta ttataaataa tatattaatt attagttaaa
     4981 caaatatata tataataaaa attgattatt tgtgttttta gtgtatagtt aacttaacta
     5041 gaaaaaagtc ttgtttcggt aaatcttctt taagtatcgc agttttagtc ataatccttc
     5101 gttttttcag attttaagtc tcattattta ttattttcaa agttattaat cagtcaataa
     5161 aatgactaaa aaattattaa aaattagtag gatatattat gtcaaactca aatgttgtta
     5221 aaggtactgt taagtggttt aacgaaacta aaggttttgg tttcattcaa caagaatcag
     5281 gtccagacgt ttttgctcat ttcagcgaaa tcgcgagttc aggtttcaag accttatttg
     5341 aaggccagca agttgaattc agtgtagctc aaggtcaaaa aggaccgaat gctgtaaata
     5401 ttgttgctat ctaagtaata ataagcagta ataaaaagca ctcataagtg ctttttttaa
     5461 gctttaaaat gttttcaaaa ttgaaaaaat ctctaaaaaa taggatataa gttgttaaaa
     5521 gtaaatgatt taattgcaaa atctaaaaat ggtactgaaa ttattgtttc attaattcct
     5581 ctacataaaa tgcaaaatac acgtcaaggt ttaaaacaaa tcgaagtagg taaaagagtt
     5641 ctactggaat ctggaatcga agttgattta aatttggatg gccgtacatt ttacacttct
     5701 ctaaatcagc tatttaaatt aaatcataaa gttgcttagc gataaatttt taaacatcaa
     5761 taatttatgt gggatttcca ctgcaatttt tttaaggaaa tatatatgtt taaacagact
     5821 ctcagcgtaa aagaccagtt tggcgttaaa cacgctattc aagccacagt ggatcatgag
     5881 tttgccaaca cacaatctca tttgaatata aaacatatca ccgttgatgg tgaagatatt
     5941 cgacctagtt ttgaaatgct ttttcaaagt accttaagtg gaaaaatatt taaaatcatc
     6001 tagaaatata gtttttataa aatctatagt ctgaatgaaa tatcaggacc tactcgctat
     6061 catgcgggtt ttttatacct aatatttata tttgtaagcc tttgcttatg cgaaacgaga
     6121 gttcacagct ctcgtttttt tattctaaaa ctgctaaaat tcttatacgc cattgacgta
     6181 cttaaaccta tgttattcat tgaaaccagt atttttacta agcaaatcaa agagcttgtg
     6241 agtgatgaag agtatcgtca gctccagcaa gatctcttgg tacagcctga taagggcgac
     6301 ttaatcaaga atggtggtgg aattcgtaaa gtacgttgtg ctcagggcaa taaaggcaaa
     6361 agtggcggta tccgtgtgat ctattattgg gttacagaag atgatcagat ctttttcctg
     6421 gtggcttatc caaagtctgt gaaagataat ctgacagaca aagaaacctc gattctacgt
     6481 caattagtaa aggagcaatt tcatggataa caacttattt gatgacctgg ttgcttcaat
     6541 caaagaagct ggtgccatca agcgtaagga gataaaggca agtcgagtca cagagcttga
     6601 attaccggat attaaagaag tacgtgagaa aacaggctta acccaggctg aatttgctgc
     6661 acgtttgcat atcagcgcga gaaccctgca aaactgggaa caaggccgtc gctatccgac
     6721 tggtcctgca gcgactttaa ttcgtatttt agatgctcat ccaactttaa tttaagaagt
     6781 ttagataata aaaaatcccg acgtcatgtc gggatttttc ggtatggagc gcataggtag
     6841 ggctgactac ctgatggtca tggtgcctga ctgattatta ttgttgtttt aagtcattga
     6901 acatcctgtc catatagatc gatcataaga gcttcatcgg gggatagata cagacaattt
     6961 cgtgaaacgc tgtaagccaa aacgtacaaa aaataatcaa aatttatccc aatggttatc
     7021 cgcagaaatt gtggattctt ttgggtaaaa aaggcttaaa aactctatgg aattgctatt
     7081 ttgatcatta tttaaccaat tttattggca agaagtatca aacttgctca aaaaaatccg
     7141 ctttggaaac aaagcggatt tttgcggtag ttttgggtta tgtaaaatag ttaagtatat
     7201 gattttattt aacttataaa agacgatttc ggataacggc cattatgtta aattgagtac
     7261 atgatacttt aagttcatag acatcttttt ttaacttaaa ataccgtatt taataagatt
     7321 tttattttat agaatatttt gtctggtctc cttacaccgc ctccagcgcg agcagctcag
     7381 ccaccgtctg ccttcgtcga ataagtcgtg gctccccatt ttccatcagc acttcggcaa
     7441 tcagcgggcg cgcgttgtag ttcgatgaca tggaggcacc atacgcaccg gtgtcgtgta
     7501 ataccagcag atctcccacc tgcgcctgag gcaaggatcg atgtaatacc acaccaccat
     7561 ctccctgcgt aaatacatct cctgactcgc acagaggtcc ggcaaccacc gtgtctaact
     7621 gtaggccatt aacaggtgtg ccatcagagt ggatcaactc catgccgtgg taggcaccgt
     7681 atagggctgg gcgaaccagg tcattgaagc cagcgtccac catcacaaaa tggttggtgc
     7741 cctgttgctt cgtagcgcgt acttcagtga ccaacacacc agactcggcc attaaatagc
     7801 ggcctggttc tatttctagt ttcactgggt gctccagtag ttgttccacg gcatcgcgcg
     7861 ctgcattcca tactgaaaag tagtgcactg tatcaatcgt gggttcaccg gtctgatagg
     7921 ggacggatag accaccaccc gcggagatgg cgtgcaggtc caaaccctgc gcattcacta
     7981 cttttaccaa cttcagcatg gcaccgcaga cttcttgcaa gtgcgtgtag tccacgcctg
     8041 agccgatgtg catgtgcagg ccatgcaaag ccaggctgtt tgacttgatc ttttcgcagg
     8101 cgcgtactag gtcgccatgc cagatgccat gcttgctatg ctctccacca gtatttgttt
     8161 tcttcgaatg gccatgcccg aaacctggat tgatgcgtaa ccacactggg tgccctgggt
     8221 tgaccgcacc caattgatcc agcatgtcca tggaaccaca gttcacagga atatttagtt
     8281 ccaccacgcg cgcaagcgta gcgcggtcca gcagatcagc cgtaaacaca atgtctgcgt
     8341 gcccgttgtg caagcccggc gtaaaaccac tagccaacgc acgttcgatt tcacccaaag
     8401 acaccgagtc caccactacg ccctgtcgct tcatgaggcg aaggatatgt gtattagaat
     8461 tggctttctg agcgaaacgc actacgtcaa acaactgcag agcggcaatc tggcgcgtaa
     8521 tggtgtcggc atcatagatc cacagcgggg tactgaattg ctgggccaaa ggagcgatgg
     8581 tagtgggagt aaaaggattc ataaatagat gatttccctt gtagtcaatt caatcataga
     8641 tgatctcctt catagtcaat tcaatcaatg ctattcttat tatttagcta ttca
//
