LOCUS       NZ_CP061570             4721 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS71 plasmid pAS71-2, complete
            sequence.
ACCESSION   NZ_CP061570
VERSION     NZ_CP061570.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092241
            Assembly: GCF_014718755.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,214
            CDSs (total)                      :: 4,118
            Genes (coding)                    :: 3,838
            CDSs (with protein)               :: 3,838
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 233 of 280
            Pseudo Genes (incomplete)         :: 44 of 280
            Pseudo Genes (internal stop)      :: 25 of 280
            Pseudo Genes (multiple problems)  :: 18 of 280
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061570.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS71"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS71-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(4661..4721,1..161)
                     /locus_tag="IC794_RS20855"
                     /old_locus_tag="IC794_20845"
     CDS             join(4661..4721,1..161)
                     /locus_tag="IC794_RS20855"
                     /old_locus_tag="IC794_20845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004993714.1"
                     /translation="MSELDFFCYSLYVQKERKYKSNWAFVIFKVRYGKWISKSLRAQAI
                     AKNPTKEYLDWLYNYFEQNLDIVKAYNS"
                     /name="hypothetical protein"
     gene            543..887
                     /gene="mobC"
                     /locus_tag="IC794_RS20860"
                     /old_locus_tag="IC794_20850"
     CDS             543..887
                     /gene="mobC"
                     /locus_tag="IC794_RS20860"
                     /old_locus_tag="IC794_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_004993722.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLSQIDAS
                     QVNAKKKNLENHYTKLDREFLLELSRIGGNINQIAKAINTDLASSRPLDAARLLHLLIG
                     IDQTLKELKK"
                     /name="mobC"
     gene            884..2800
                     /locus_tag="IC794_RS20865"
                     /old_locus_tag="IC794_20855"
     CDS             884..2800
                     /locus_tag="IC794_RS20865"
                     /old_locus_tag="IC794_20855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045126957.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPFAKKYTSGCLSFYEHDLSDQDKQQIMQNFEQALFPGMDQSQYRVLWIEHQDKKNEE
                     TGKRRLELNFLIPNIEILTGQRLQPYFDRADRSRVDLFKKITNYEYQLHDADDPLYQQD
                     IKHAKNLPKAVNEIKETLNILATKAVENGLITDRQSMKTWLLDLGLEISRETKKSLSIK
                     NPNNDDPNARPIKLKGAIYEQDFRLTEESERLTIAASERYRREAEQRNQSNIQRYAEYS
                     AKRSTELEQQYRQHQNRYTAETERSNERDITADRPDYSSNQEATATGHDSPIARTTTAD
                     QTATSELAAVERLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRH
                     HSNGEQSQLFKITGREHDNNNVRGETVRGVRPDSERELRIGQWNETAWNSKGGTLNESR
                     STVIADYRAATAAAQRATEAARASIAAHSGTEQNHRRIRELQQQTGEALQPVERERKRA
                     DQYHQETTESEVIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRNIANDDLYRNRE
                     ADSTIDGTASKETRLSATVSRTIRGFDQENIFKALDELDRRRELQIVQERKNDRGYDSP
                     SPF"
                     /name="hypothetical protein"
     gene            2872..3111
                     /locus_tag="IC794_RS20870"
                     /old_locus_tag="IC794_20860"
     CDS             2872..3111
                     /locus_tag="IC794_RS20870"
                     /old_locus_tag="IC794_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005000146.1"
                     /translation="MELAIKKWGNSAAVRLPSILLESLNLKLDSRVEVFTQDEAIVIKP
                     IKSKISLEQLLADITSDNLHCEVDTGHAVGKEIY"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            3111..3452
                     /gene="mazF"
                     /locus_tag="IC794_RS20875"
                     /old_locus_tag="IC794_20865"
     CDS             3111..3452
                     /gene="mazF"
                     /locus_tag="IC794_RS20875"
                     /old_locus_tag="IC794_20865"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_005000144.1"
                     /translation="MPQNYVPEEGDIIWINFNPQSGHEQAGHRPALVLSPSSYNKATSL
                     LVCCPMTTQIKGYPFEVKIVGNPENVALSDQVKSLDWVARSATKKGQAKSEELEEVRQK
                     IAALLKIST"
                     /name="mazF"
     gene            3714..4664
                     /locus_tag="IC794_RS20880"
                     /old_locus_tag="IC794_20870"
     CDS             3714..4664
                     /locus_tag="IC794_RS20880"
                     /old_locus_tag="IC794_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004993712.1"
                     /translation="MNNAQLTDFFTNLAHKPYCADELLYGLKIRPKKTAINMQYIQGNQ
                     PCMLHYFFFDIDRSDAVMAWHEENLPVPYWTAQTQKNGHAHLCYKLEIPLCTSEFGSQK
                     SIAYASKVQAALANKLGADVGYSHLITKNPFHKDWRVTFWSEQAYTLDYLADFVELPKK
                     LSKKQEVLGLGRNCTLFENARKWAYKAVRDYFHHYSSLEWDKAVLAHLEALNREFEVPL
                     PYSEVKATAKSIAKYCWNKFSYAGFSEWQSKNAERANAKGACSLGGKARSQQFNNLRQQ
                     ALDLHIEGVNNTKIAEYLNVSRKTITRWLNQVVIQ"
                     /name="replication initiation protein"
     Typing          885..2797
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.011"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaactgggc atttgttatt tttaaggttc gctatggtaa atggatatct aaaagtctta
       61 gagcacaagc aatagcaaag aatccaacaa aagaatattt agactggcta tataactatt
      121 ttgaacaaaa tttagatatt gtgaaggcat ataattcata aaaaggggtg ggacagtgcc
      181 gccatatcag atatcagccc cttgggtgct tttttcccat ttcattttta aaaattatta
      241 tttaagggaa tgagcaaggc tgtgggcaaa gtctcgacct gtgtaataaa cttgtgtgca
      301 actcaacgta taactagaag agttatgcgg agttgtgcat aatgtttatt cacagattag
      361 tcgaggcgtt cgcttgcgat tgtccataac cttgccatgc cctgctcaat aaaatatacg
      421 tctaggggtc tgtcaccgta tgagaacgca aggatacatg gagacccctc cgctacgcta
      481 cagagttcca tgaccttgtt caggggcaag cccccgaaac ccccaaaagc aaaggcaaaa
      541 gcatgacagc gatcaaacgt aataaggttt acaaagtccg attttctgat gcagaggtac
      601 agaaaatcaa agatgccaac atcctaaata ttgctaaata tttgcgggat acttcgcttt
      661 ctcaaattga tgccagtcaa gtcaacgcca aaaaaaagaa tttggaaaat cactacacaa
      721 aattagatcg tgaattttta cttgagctat cccgaatcgg gggcaacatc aatcagattg
      781 caaaggcaat aaataccgat cttgcaagca gtcgtcccct tgatgctgca aggcttttgc
      841 acctgttaat agggattgat cagaccttaa aggaattgaa aaaatgattg ttcagttctt
      901 caggtacggc aatggtttaa gcaaagggcc actagattat ttgcttggta aagaccgtga
      961 tagagactat gcaaaagttt tgcagggtga tgtttccgaa gtatcagggt taattgatac
     1021 atcacctttt gccaaaaaat atacgtcagg ttgtttgtct ttttacgagc atgatttaag
     1081 tgatcaagat aaacagcaga ttatgcaaaa ttttgagcaa gccttattcc ctggcatgga
     1141 tcagagccag taccgtgttt tgtggattga gcatcaagac aagaaaaacg aggagacggg
     1201 gaagcgaaga ttagagctta attttctaat tcccaatatt gaaattctta cagggcaacg
     1261 tttacagcca tattttgacc gagctgatcg ttctagagtt gatctattta agaagattac
     1321 aaactatgaa tatcaactgc atgatgcaga tgacccgttg tatcaacaag acattaaaca
     1381 cgccaaaaac ctacctaaag ctgttaatga gattaaggaa actctcaata ttttagctac
     1441 gaaagctgta gagaatggat taattacaga tcgtcaaagc atgaaaacgt ggctattgga
     1501 tctcggtctt gaaatcagca gagaaacaaa aaaaagccta tctattaaaa atccaaataa
     1561 tgatgaccca aacgccagac caattaagtt aaaaggtgca atctatgagc aggattttag
     1621 acttacagaa gaaagcgaac gacttactat tgcagcatca gagcgatata gacgagaagc
     1681 tgaacagcga aatcagtcaa atattcaaag atatgcggaa tacagtgcga agcgaagcac
     1741 agagcttgag cagcagtatc gacagcacca aaaccgatat acagcagaaa cagagcgaag
     1801 caacgaacga gatattacag cagatcgacc agactacagc tcaaaccaag aagcaacagc
     1861 aacagggcat gacagcccta ttgcaagaac aacaacagca gatcaaacag ctacaagcga
     1921 acttgcagca gttgaacggc ttgagccaag aaacagccca agcagccaaa ttaaagaaaa
     1981 cccttattac atcgaatata gccttgattt cactggcatg tataacgctt atcagcagta
     2041 tttgtctggg gtacgtcagc aagagcaaat acaacgacat cacagcaatg gagaacaaag
     2101 tcaattattt aagatcacag ggcgggagca tgataacaac aacgtgcggg gggaaactgt
     2161 gcgtggagtt agacccgaca gtgaaagaga actacgcatt ggacaatgga acgagaccgc
     2221 ttggaatagt aaaggaggta cgttaaatga atctcgaagc acagttattg cagattatcg
     2281 agcagcaaca gcagcagctc aaagagcaac agaagcagca agagcaagca ttgcagctca
     2341 ttcaggcaca gaacagaacc atagacgaat tagagaacta caacaacaaa ctggtgaagc
     2401 actgcaacca gttgaacgag aaagaaaacg agctgatcaa tatcatcaag aaactacaga
     2461 atccgaagtt atcagaggat ttattgcaag gcttggagaa cagcttaaaa cagcgattac
     2521 agagccattt agagcaatta gtgactgggt taaatctaaa gaacgagatc aacgaaatat
     2581 tgccaacgat gatctataca gaaatagaga agcagacagc accattgacg gaacagcttc
     2641 aaaagaaact agactcagtg caacagtatc acgaacgatt cgaggttttg atcaagaaaa
     2701 tatcttcaag gctttagatg agttggacag acgtagagag ttgcaaatag tgcaagaacg
     2761 taaaaatgat cgaggttatg attcaccaag tccattttaa tgctcgcgtt attgacacaa
     2821 aaatcttgct agggtattgt atctacaatg tagatactag gggttgctgt tatggaacta
     2881 gctattaaaa agtggggcaa tagtgctgct gtccgcctac catccatttt attagaaagc
     2941 cttaatctta aattggatag ccgtgttgag gtatttactc aagatgaagc gatcgttatc
     3001 aagccaatta aatcaaaaat atctttagag cagcttttag cagatataac atctgataat
     3061 ttgcattgtg aagtggatac tggacatgct gtaggtaagg aaatctatta atgcctcaaa
     3121 attatgtacc agaagaaggc gatattatct ggattaattt taatccacag agtgggcatg
     3181 aacaggcagg acatagacct gccttagttt taagtccttc atcttataat aaagccacaa
     3241 gcctactagt ttgctgtcca atgacaacgc agataaaagg gtatcctttc gaagttaaaa
     3301 ttgtaggaaa tcctgaaaat gtagcactat ctgatcaagt aaaaagttta gattgggtcg
     3361 ctcgatcagc aacgaaaaag ggacaagcta aatctgaaga acttgaggaa gtgcgacaaa
     3421 aaattgcagc cttactgaaa atatcaactt gatatcatct atgaaatcaa agggtaaggt
     3481 aatacataaa tctttttaag attatttact gatacaatag cctatataaa tcaataagtt
     3541 ggaaagttta atgaagtgtg ttaaattttc ttttaggcga aaaaaaagca agcttgccgg
     3601 ctcacttttt tctcattgtt taacaaatac ttaaagcatc attaaacgca aattagttac
     3661 ttgttatttt actgatgttt tgagcatttg caacctttag aacgttgcat aatatgaata
     3721 atgctcaatt aacggatttt tttactaatc ttgctcataa accatactgt gcagatgagt
     3781 tactgtatgg cttaaaaata agaccgaaaa aaacggcaat caacatgcaa tacatacagg
     3841 gcaatcagcc ttgtatgctt cattacttct tttttgacat agaccgttca gatgcagtca
     3901 tggcatggca tgaagaaaat ttaccagtgc cttattggac agcacaaaca cagaaaaacg
     3961 gtcatgctca tctctgttat aaattagaaa ttcctttgtg taccagtgag tttggcagtc
     4021 aaaaatcaat agcctatgct tcaaaggtac aagcagcgct tgccaacaaa cttggtgcag
     4081 acgttggcta tagtcatctc attacgaaga atccatttca taaagattgg cgtgtcactt
     4141 tttggagtga acaagcgtat acgctcgatt atttagccga ttttgtagag ttaccaaaga
     4201 aattaagcaa aaaacaggaa gttttagggc ttggtcgtaa ctgcacctta tttgagaatg
     4261 ctcgtaaatg ggcatataag gctgttagag actactttca ccattatagt agtctagagt
     4321 gggacaaagc tgttttagcg catctagagg cattaaatcg agaatttgaa gtacctttgc
     4381 catatagtga agtaaaagct acagcaaaga gtattgctaa atattgttgg aataagtttt
     4441 cgtatgctgg atttagtgag tggcagtcta agaatgccga acgtgctaat gctaaagggg
     4501 cttgttcttt gggaggaaaa gcacgctcac aacaattcaa taatttaaga caacaagcct
     4561 tagatttgca tattgagggt gttaacaata ctaaaattgc tgaatattta aatgtaagcc
     4621 gtaagacgat tactcgttgg cttaatcagg tagtaattca atgagcgagt tagatttctt
     4681 ttgttattca ctatatgttc aaaaagaacg taaatataaa t
//
