LOCUS       NZ_CP061564             4394 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS73 plasmid pAS73-3, complete
            sequence.
ACCESSION   NZ_CP061564
VERSION     NZ_CP061564.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092243
            Assembly: GCF_014718355.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:23:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,004
            Genes (coding)                    :: 3,766
            CDSs (with protein)               :: 3,766
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 107 of 238
            Pseudo Genes (incomplete)         :: 133 of 238
            Pseudo Genes (internal stop)      :: 30 of 238
            Pseudo Genes (multiple problems)  :: 27 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061564.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS73"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS73-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TSGH"
     gene            complement(695..910)
                     /locus_tag="IC796_RS20265"
                     /old_locus_tag="IC796_20255"
     CDS             complement(695..910)
                     /locus_tag="IC796_RS20265"
                     /old_locus_tag="IC796_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004847384.1"
                     /translation="MQNELERYKNFDPTNAPLVKPSFVKKLQERHAIAQAQAFDADVVA
                     WLSSQDNETKQHINDMVRHIMALKRA"
                     /name="hypothetical protein"
     gene            complement(900..1196)
                     /locus_tag="IC796_RS20270"
                     /old_locus_tag="IC796_20260"
     CDS             complement(900..1196)
                     /locus_tag="IC796_RS20270"
                     /old_locus_tag="IC796_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_004846796.1"
                     /translation="MPLIEYYGLLFEWHDQKMDLVYRGREITFEEVCSIFLDPALMSFE
                     DVGHYDEQRLISVGLSNRGRLLTVVWVERGDVARIITAFEPSHHQKRRYSNAK"
                     /name="BrnT family toxin"
     gene            complement(1237..1737)
                     /locus_tag="IC796_RS20275"
                     /old_locus_tag="IC796_20265"
     CDS             complement(1237..1737)
                     /locus_tag="IC796_RS20275"
                     /old_locus_tag="IC796_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151686086.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVTQAVKELQITEERIQKVIAGQTH
                     STVSKSLNTGLQEGTAELIKTAKALGHLKNSIESTSDQFSWKIITIILGVFAILMLGII
                     WLFSVYIKPLENIPQIESLRQQGIQFDIGSCTVGEESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     gene            complement(1734..2663)
                     /locus_tag="IC796_RS20280"
                     /old_locus_tag="IC796_20270"
     CDS             complement(1734..2663)
                     /locus_tag="IC796_RS20280"
                     /old_locus_tag="IC796_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_151686088.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQAETDLEHSAY
                     GNMPKWAEHNPINFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGNDQPHVHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDN
                     TGKSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKEPEKKLLPSQA
                     KDPEIREALQQSRTAYKELERLDLGDPKNDLKDLKDSPISDKEIKQGIESFKADFDSFK
                     QLSLQQYKEQQKLEREQQKTMSYKGMSR"
                     /name="MobA/MobL family protein"
     gene            complement(2663..2818)
                     /locus_tag="IC796_RS20285"
                     /old_locus_tag="IC796_20275"
     CDS             complement(2663..2818)
                     /locus_tag="IC796_RS20285"
                     /old_locus_tag="IC796_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_018680092.1"
                     /translation="MQLKQVLGKIKDIVFGVIVAPLLIIIMMWASILDQRAFKRELDRK
                     KGQDKT"
                     /name="hypothetical protein"
     gene            3099..3509
                     /locus_tag="IC796_RS20290"
                     /old_locus_tag="IC796_20280"
     CDS             3099..3509
                     /locus_tag="IC796_RS20290"
                     /old_locus_tag="IC796_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_151686090.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKLNA
                     LLKAEKAIDRANKQKIAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     VLLKTAEIDPNDMKWQIWKDLGQETLNKRKIT"
                     /name="conjugal transfer protein TraD"
     gene            3559..4275
                     /locus_tag="IC796_RS20295"
                     /old_locus_tag="IC796_20285"
     CDS             3559..4275
                     /locus_tag="IC796_RS20295"
                     /old_locus_tag="IC796_20285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151686092.1"
                     /translation="MGLFPCEFGFPFNKDSFKSIFFTNNDYSSRSVSFELEFKNSFNFD
                     AVLFTENNLEDNSHYEYAKHTDYEIDVNLAPYAEFAKLSIHISDKESGKVDFDVLNNTF
                     WISIYLSEKDFDALIPTIKDKDIYIDFNILCTKSDDDVEDTIKKLIVDDVKKQVLVLNK
                     FVKSVKIGKLTLTAKNPKLEKIESDENQLENNKEDLNKELISKASTTNQLLTQLLKKQE
                     MIFYIIIALLILIFFK"
                     /name="hypothetical protein"
     Typing          complement(1738..2663)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.793"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttcggcaa tactctccct aaaggcggtc atttaaaaaa atgacgcatc ctttaagtat
       61 tgccgattta ccgcatattc ttttggcttc ggagccacgt atatcggtcg tatccgcaca
      121 tagctttgca aactatgcag acaaggacta aaggtcttgc acctctcccc acacgctccc
      181 tgtcgaagta tggcggtatc tctgcgaggg tttccccaac atacaactta accaacctat
      241 acaactactc ttaacgctcc gcttttgtga gattcccctg tagcggttta gcctttcgac
      301 tatcgctacg ttgcaccagt gacacttatt ccctccacat gcaacgcatg ccaatccgta
      361 aaacggacac gcaggagtag gcaaagctcg atctaggatg cttatttgat gatttaaaac
      421 acggattaac gaagacttat cattttgggt cttgaactaa acactgttta ttgtgaaaat
      481 agagttcagg tacaagcctt agtgtgaaat ctgcacgagt tggtagctct gctgatcttc
      541 gttattcgat ggttcagaag catcaaataa ctgcgtaccg ctgaatataa agcgaaagcc
      601 cattgagttg caagattcaa tgggcttttt ctttgatcaa tcagacatta agacaaaaaa
      661 gggaggtcat atctaatcat gactaaatcc ctaatcaagc acgtttcaat gccataatat
      721 ggcgaaccat atcgttaata tgttgttttg tttcattatc ttgtgaactt aaccatgcaa
      781 ctacatcagc atcaaaagcc tgtgcttgag ctatggcatg gcgttcctgt aatttcttca
      841 caaagctagg tttaaccaat ggcgcattgg tagggtcaaa atttttatag cgttctagtt
      901 cattttgcat tgctgtacct ccgcttttgg tgatgtgatg gttcaaatgc agtgattatt
      961 ctcgcaacat ctccacgttc aacccaaacc actgttaata gtctaccacg attgcttaaa
     1021 ccgactgata ttagtcgttg ttcatcatag tgtcctacat cctcaaaaga catcaatgca
     1081 ggatcaagga aaatactaca gacctcctcg aacgtaattt cacgccctcg atagactaaa
     1141 tccatttttt ggtcatgcca ttcaaataac aagccgtaat attcaattaa tggcatgatt
     1201 acctcttttc ctctcgtgaa ataaaaaata tgcctattat tttgggtcta tgacacaaaa
     1261 atctccattt tttccatatc cgcactgctt tttcatcact cgaacacacg gtttagattc
     1321 ctctccaaca gtacagcttc ctatatcaaa ctggattccc tgctgtctta agctctcgat
     1381 ctgcgggata ttttctaaag gtttgatata aacactgaat aaccaaatga tgccaagcat
     1441 cagaatggcg aaaacgccta aaataatggt gatgattttc catgaaaatt gatcactggt
     1501 tgattcaatc gagtttttta aatgtccaag tgctttggcg gtcttaataa gctccgctgt
     1561 accctcctgt aagcccgtat ttagcgattt tgatacggta ctatgggttt gccctgctat
     1621 gaccttttga atgcgttctt cggtgatttg aagctcttta acagcctgag tgactaggtt
     1681 ctgctgttgt tctgcaactg ccatcagtgc atataacttt ttttcatcag gtgtcatcgg
     1741 ctcatcccct tataactcat ggttttctgt tgctctcgtt ctaatttctg ctgttctttg
     1801 tactgctgta aggaaagttg tttgaagctg tcaaaatcag ccttaaagct ctcaatgccc
     1861 tgtttaattt ctttgtctga aattgggctg tctttgaggt ctttcaggtc atttttaggg
     1921 tcgcctaagt ctagtcgctc aagctcttta taggctgtac gtgactgttg caaggcttct
     1981 ctgatttcag ggtctttagc ttggcttggt aatagtttct tttcgggttc tttctctatg
     2041 ccttgctctt tgtagcttcg catatcaata cggctatcaa tttggtgttt ttctaaatgg
     2101 ctgttgcata aatctgccca tctttggcgt aggtctttaa tatcagtttt tcgttcctgg
     2161 taactcttgc ctgtattgtc ttttttagct ccgccacgtt caggattttt gctgttatag
     2221 cgtttgaaat attgctctgg atctcgctcg attccatctt gcagacgttc attaaacatg
     2281 aggtgaacat gaggctgatc attgccgtcc attgctttag ggttatgaat cgcaaactga
     2341 tacggatatt ttgagccaat ttcagactga atgaagtcct caaccagttc taagcgttgc
     2401 tcggcattca tctctctagg caaagctatt tcatactctc ggtaggtact gccgttttta
     2461 cgctcgtaaa ggtcggctgc ctgccaaaag ttaatcggat tatgttcagc ccatttcggc
     2521 atatttccat aggcactatg ttcaaggtcg gtttctgcct gctcctgttt cagcttcttt
     2581 tcttcatccc tatcaatata ttcggcatgc ggtgctgctt tgcctgcttt accaactttg
     2641 acacttaaac gagcaatcgc cattaggttt tatcctgtcc cttttttcta tccagttctc
     2701 tcttaaatgc tctttgatcg agtattgaag cccacatcat aatgatgata agtagcggtg
     2761 ctacgatcac gccaaaaaca atatctttga tctttcccaa tacctgtttt agttgcatgc
     2821 ggttgctcct agctttggta cttctttttt tcgttcaatt ttcaattgaa cgaaaaaaag
     2881 aagtttcagg gttttaggga tgaagtccct aacgtgctta ggtttctcaa tgaaattgag
     2941 aaacgtctaa gcgcgccatc aaatttttga tggaagtaca ggcgacaatc tctagaacga
     3001 ctttaacaaa attacgttac tcgaacgtaa atattttgtt atcgttcttt gagtattgag
     3061 cctgtgcttt tgcggtatct gaattaatgg aattgaatat gaataatgat tggctgtctg
     3121 aaaaaatcac ctatatttcc gctttgaaga atcctagtga tgctcaaaag cttttattag
     3181 aattagctaa aatccaatac cgtacacccg accaagagaa aaaactgaat gccttgctca
     3241 aggctgaaaa agccatagat cgagcgaata agcagaagat agcagttaga aaattactga
     3301 atgcagaaaa agaagctgaa cgtaaagctc gtacacgtca tctcatccaa ttaggcgcac
     3361 tttttgaaat cgctaaccta gatcagcgtg acccagccga attacttgga gttctgctta
     3421 aaactgctga aatcgaccca aatgatatga aatggcagat ttggaaggat ttaggacaag
     3481 aaaccttaaa taaacgcaaa attacttaat tttattttga tttttaccat acatctttaa
     3541 ctaatattgg aggttaattt gggacttttt ccatgtgaat ttggttttcc ttttaataag
     3601 gattcattca aatctatatt ttttactaat aatgattatt caagtagatc agtatctttt
     3661 gaattagagt ttaaaaattc ctttaatttc gatgcagttc tttttactga aaataatcta
     3721 gaggacaata gtcattatga gtatgctaaa catacagatt atgaaataga cgtcaatcta
     3781 gctccttatg ctgaattcgc taaattaagc attcatattt ctgataaaga atcaggaaaa
     3841 gttgattttg atgttttaaa taatactttt tggatttcaa tttatttaag tgaaaaagat
     3901 tttgatgctc taatcccaac aattaaagat aaagatattt atattgattt caatatttta
     3961 tgtactaaat ctgatgatga tgttgaagat actataaaaa aactaatcgt tgatgatgtc
     4021 aaaaaacaag ttttagtatt aaataaattt gtcaaatctg taaaaatagg aaaattaaca
     4081 ttaactgcta aaaatccaaa gttagaaaaa attgaatctg atgaaaatca gcttgaaaat
     4141 aataaagaag atttaaataa agagttaata agtaaagctt caactacaaa tcaactctta
     4201 actcagttat taaaaaaaca agaaatgatc ttttacataa taattgcatt attaatactt
     4261 atatttttta aatagcctaa gcataaaata aggaatagct cctatccctc actccaaaaa
     4321 gaaaaagctc gaactctctc gcccgccccc aaagcgtcgc acttcgtgct ttggctttgg
     4381 aaacggttga gaga
//
