LOCUS       NZ_CP061560            12797 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS74 plasmid pAS74-4, complete
            sequence.
ACCESSION   NZ_CP061560
VERSION     NZ_CP061560.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092244
            Assembly: GCF_014717935.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12797)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:18:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,221
            CDSs (total)                      :: 4,126
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 366
            CDSs (without protein)            :: 366
            Pseudo Genes (ambiguous residues) :: 0 of 366
            Pseudo Genes (frameshifted)       :: 300 of 366
            Pseudo Genes (incomplete)         :: 64 of 366
            Pseudo Genes (internal stop)      :: 24 of 366
            Pseudo Genes (multiple problems)  :: 19 of 366
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061560.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12797
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS74"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS74-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TSGH"
     gene            12..236
                     /locus_tag="IC797_RS20840"
                     /old_locus_tag="IC797_20835"
     CDS             12..236
                     /locus_tag="IC797_RS20840"
                     /old_locus_tag="IC797_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072934.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAVVISDMKDDGLDFYPIFEKVRAYSDQ"
                     /name="hypothetical protein"
     gene            complement(315..713)
                     /locus_tag="IC797_RS20845"
                     /old_locus_tag="IC797_20840"
     CDS             complement(315..713)
                     /locus_tag="IC797_RS20845"
                     /old_locus_tag="IC797_20840"
                     /inference="COORDINATES: protein motif:HMM:NF014599.3"
                     /GO_function="GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /GO_process="GO:0051262 - protein tetramerization [Evidence
                     IEA]; GO:0015031 - protein transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="WP_191106408.1"
                     /translation="MNIQLTSTKTLFLKLQPPQDNFIEQEEKFSFDYQVEFPESIKNKF
                     IVVIKSLIEHKSGYHIDTIFLAEFKIDSDINEEFKNSKFPFVNAPAIAYPFFRAFIANT
                     LVNCGLEVMYLPSVNFEAMYKKKKEKSV"
                     /name="protein-export chaperone SecB"
     gene            complement(717..1133)
                     /locus_tag="IC797_RS20850"
                     /old_locus_tag="IC797_20845"
     CDS             complement(717..1133)
                     /locus_tag="IC797_RS20850"
                     /old_locus_tag="IC797_20845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191106409.1"
                     /translation="MSSYSNDCIQNAQAWIDEIDDIDQFLDQLIEVNENCASSPSLDAF
                     CASFSLNTTESLISSAMELSSFSRINSVPSEYLATMSTMSIPTDLSKLLISLSTERTED
                     SLCFDMSELAYNPTETPIGNFKTQEVSFQYKFAA"
                     /name="hypothetical protein"
     gene            complement(1152..1751)
                     /locus_tag="IC797_RS20855"
                     /old_locus_tag="IC797_20850"
     CDS             complement(1152..1751)
                     /locus_tag="IC797_RS20855"
                     /old_locus_tag="IC797_20850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191106410.1"
                     /translation="MILDLHLNCFHTCKLDGGKDSIISKAPFPSNPQQQQWLGQGFYLW
                     TDSDFFAHEWGKDHYQSNYAINQFEVRVPKDLFWDLVGNVQHQIEFVNFKDQFHCLLDE
                     LVERATPARKQQTQQKVQRLKQQEIKVSTLFWVLRHLRKLAYKVVKASDIKSKKTESIE
                     FIGGKGGECIFLPTRQQIVVYPESKYMIHHIDWVHP"
                     /name="hypothetical protein"
     gene            complement(1874..2419)
                     /locus_tag="IC797_RS20860"
                     /old_locus_tag="IC797_20855"
     CDS             complement(1874..2419)
                     /locus_tag="IC797_RS20860"
                     /old_locus_tag="IC797_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_191106411.1"
                     /translation="MPKLKNDTHPEILQLGAFLNNFNQESDRGAALSAAAVLEDRLEEI
                     IKAFLIDGKSSLKLLDGFNAPIGTFSSKILLAHALGLLQDDEFQQIELLRRIRNQFAHT
                     WEYLNFESNSIKSLVFTLPYMGPSDIKEDCRSKPRQYFNFWVAGILINLLWRKNFVIKE
                     KRSNKEYIQLYKVDADTI"
                     /name="transcriptional regulator"
     gene            2480..2857
                     /locus_tag="IC797_RS20865"
                     /old_locus_tag="IC797_20860"
     CDS             2480..2857
                     /locus_tag="IC797_RS20865"
                     /old_locus_tag="IC797_20860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191106412.1"
                     /translation="MTTEIFKAGTQYNDFTGTSAAETQGFVEYLKSKDLIKDDEFVIGI
                     DFSPLVGISKDQNEIFLQVYLTKDFSYDEYEAKSRNDSVLEIRKIETYLDIVKFFSLFK
                     RLNVSFSWQGLLENKEIKIIN"
                     /name="hypothetical protein"
     gene            complement(2929..3243)
                     /locus_tag="IC797_RS20870"
                     /old_locus_tag="IC797_20865"
     CDS             complement(2929..3243)
                     /locus_tag="IC797_RS20870"
                     /old_locus_tag="IC797_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005098515.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(3230..3517)
                     /locus_tag="IC797_RS20875"
                     /old_locus_tag="IC797_20870"
     CDS             complement(3230..3517)
                     /locus_tag="IC797_RS20875"
                     /old_locus_tag="IC797_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_191106414.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETTSLVFDDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDRYEHG"
                     /name="BrnT family toxin"
     gene            4028..5515
                     /locus_tag="IC797_RS20880"
                     /old_locus_tag="IC797_20875"
     CDS             4028..5515
                     /locus_tag="IC797_RS20880"
                     /old_locus_tag="IC797_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005108963.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPGMISAATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLVII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTTSDKNRECKGQGVANVASGFLGGMAGCAMIG
                     QSIINVKSGGRTRLSSFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            5528..6379
                     /locus_tag="IC797_RS20885"
                     /old_locus_tag="IC797_20880"
     CDS             5528..6379
                     /locus_tag="IC797_RS20885"
                     /old_locus_tag="IC797_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_191106413.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLNSPLQLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLNELVELEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGTETEHNSSKVGTHIENVVRALHKPVLITSSPFSPP
                     QSFLIAFDGSPTARICVDKIANRPLLKTLTAHVVYVGKPNSDMTSQVTWAKELLANNGF
                     DVIDSVLEGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(6612..7178)
                     /locus_tag="IC797_RS20890"
                     /old_locus_tag="IC797_20885"
     CDS             complement(6612..7178)
                     /locus_tag="IC797_RS20890"
                     /old_locus_tag="IC797_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000858429.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPEKTENKR
                     IPVPEPVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(7242..8123)
                     /gene="repM"
                     /locus_tag="IC797_RS20895"
                     /old_locus_tag="IC797_20890"
     CDS             complement(7242..8123)
                     /gene="repM"
                     /locus_tag="IC797_RS20895"
                     /old_locus_tag="IC797_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            complement(8672..9841)
                     /locus_tag="IC797_RS20900"
                     /old_locus_tag="IC797_20895"
     CDS             complement(8672..9841)
                     /locus_tag="IC797_RS20900"
                     /old_locus_tag="IC797_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_043041652.1"
                     /translation="MASYHFSVKSKNKGYAIGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIEFWQAADSHERANAKVYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHENYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDDQDITIKNQVSADSYEKY
                     NEIHSTLYLAEPKLGAGQQNATPEYLAQIQKIREHNHQERKREERQKQLDQKHEYFYSA
                     ENHPFSSLEVMYHLNYLIKNAKNKEKLEKDFSEIIKIQEPILHQEGWNLNNMNRKLRDS
                     EHNSTPRFEFEVHSQPNYPLPMQNKPPQPVQPEQPKPKSDDYDYGGSGF"
                     /name="MobA/MobL family protein"
     gene            10147..10899
                     /locus_tag="IC797_RS20905"
                     /old_locus_tag="IC797_20900"
     CDS             10147..10899
                     /locus_tag="IC797_RS20905"
                     /old_locus_tag="IC797_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191106407.1"
                     /translation="MTLEKAKKRGRPSQLLQMAELHAFVEFLLEKDPRSELQNQVIDAL
                     QAEDFNFEMLSEAQQILVKEALKPYREHLKLQLLFDELVITPGRTEYEEKFLELYQDYK
                     KDDLDPAEMNILKTMCTRYLNFKAQKLEYSDLELYLSQIQKKYAGKKRKAENQRKFEVG
                     GGVLSLYKVKDDENLDSKKFLKRIEIDRFLAGKFRQSQLYKKVWALERPNTEKYQLLFE
                     VLHQLSGYSLKGQTLTLLDLVIIDAQKR"
                     /name="hypothetical protein"
     gene            11147..12346
                     /locus_tag="IC797_RS20910"
                     /old_locus_tag="IC797_20905"
     CDS             11147..12346
                     /locus_tag="IC797_RS20910"
                     /old_locus_tag="IC797_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072939.1"
                     /translation="MTPKILCIKSLELEVEKIMSIQEFAFQLSKKIQEQHSVKISRSHI
                     YELIALDQDYRTYSSFVAQNLLLDAQYDDSEEYYQHELVDALTLEILENPPETDYLNYS
                     EDDLHWDDYEGNVLLEQIRKIIIRLKVLLKLDASEEFYLSISKTVYRELLWLNLEVINF
                     NAVREELSYINFENGKVDDLELGGDYFEFSKIGAIFEKILSYAQDRENADAYALLGAYY
                     RYLANQIAPYGRHGSNFGSRWDNNKQKYINSDETKKNKEKYEEYIKQAEHFESYIKNSP
                     MNLHEINLDADKETVYNQILYLCNRGDTSAIEYFLYEEIFKNHGEAWLYIYLAQMCGVD
                     FTQDDFRDYNAYTGEDYDDYGPIEIAGREAIQYAIDLSELDVDKDNLARKIAAELFEKI
                     "
                     /name="hypothetical protein"
     gene            complement(12418..12660)
                     /locus_tag="IC797_RS20915"
                     /old_locus_tag="IC797_20910"
     CDS             complement(12418..12660)
                     /locus_tag="IC797_RS20915"
                     /old_locus_tag="IC797_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001015364.1"
                     /translation="MNHFVQPKLLISSQAKKIGLALINDLELCITARLAFYLDVNPSYD
                     GHEATFIWIEYFLQDHPQAEFSEVREAFLQLIPGC"
                     /name="hypothetical protein"
     Typing          complement(7245..8123)
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.922"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8727..9841)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.656"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttttaaaac tatgccttac gaagaatttc aaagacttat aggtaagtct ggcctttcta
       61 ttaaagaatt tgcagcgctt ttagatatga acgcaaattc aattaccaac tataaaaaga
      121 acggtaaagt gcccactacg attgcagtaa ttgcagtcgt aatttcagat atgaaggatg
      181 atggtctgga cttttatcca atttttgaga aagttcgagc ttatagtgat caatagagta
      241 tttgatgtga acccaattaa agatttaatt ggatcctagc acgtgctgct gcatttcgta
      301 gatgagattt tatgttaaac ggacttttct tttttcttct tgtacatggc ttcgaagttc
      361 accgaaggta aatacattac ctctagacca cagtttacca acgtattagc tataaatgct
      421 ctaaagaatg ggtaagcaat tgcaggagcg ttaacaaatg gaaattttga atttttaaac
      481 tcttcattga tgtctgaatc aatcttaaac tcagctaaaa aaatagtatc aatgtgataa
      541 ccgcttttgt gttctatcaa acttttgatg actacaataa atttattttt aattgattca
      601 ggaaattcaa cttgataatc aaaactaaat ttttcttctt gttctataaa attatcttga
      661 ggtggttgta gtttcaagaa taaggtttta gttgatgtta gttgaatatt catattttac
      721 gcagcaaatt tatattgaaa ggaaacttct tgagttttga agtttcctat aggtgtttct
      781 gttggattat aagctagttc agacatatca aaacataagc tatcttcggt tctttctgta
      841 gaaagagaaa ttaataactt agataagtca gtaggaatag acatagtcga cattgttgct
      901 aagtactcag aaggaacaga atttattctc gaaaatgaag acaattccat tgctgaacta
      961 atcaaacttt ctgtcgtatt taatgaaaaa cttgcacaaa atgcatctaa agatggtgaa
     1021 gatgcacaat tttcatttac ttcaatcaat tgatcaagaa attggtcaat atcatctatt
     1081 tcatcaatcc atgcttgtgc attttgaatg caatcatttg aatatgaaga catagttctc
     1141 tccaatagga gttaagggtg tacccaatct atatgatgta tcatatattt agattctggg
     1201 taaacaacaa tttgttggcg agttggtaag aatatgcatt cccccccttt gccaccaata
     1261 aattcaatag attcagtttt cttagattta atatcagatg ctttaactac tttgtaagct
     1321 agtttgcgta agtgtcttaa aacccaaaat aaagttgata ctttgatttc ttgctgtttt
     1381 agacgttgaa ctttctgttg tgtttgctgt ttcctagctg gagtagctcg ctcaacaagt
     1441 tcatctaata aacaatggaa ctgatcttta aagttaacaa actcaatttg atgttgaacg
     1501 tttcccacta gatcccaaaa aagatcttta ggaactctta cttcaaattg atttatggca
     1561 tagttagatt ggtagtggtc cttgccccat tcatgcgcaa aaaaatcact atcagtccaa
     1621 aggtagaacc cttgtccaag ccattgttgc tgttgtggat tactagggaa aggtgctttt
     1681 gatattatag aatcttttcc accatctaat ttacaagtgt ggaaacaatt caaatgtaaa
     1741 tctaatatca atgtatccta ctccacctcc tcagtgttgc gaattatata ggtaatattc
     1801 tgaaatttat atatttgata taaatatata tatgacgcat acgcctattt aagttcatat
     1861 aaagcccatt atcttaaata gtatcagcat ctactttata aagttgtata tattctttat
     1921 ttgacctttt ttctttaatt acgaaatttt tacgccatag aagattgatt agaattcctg
     1981 caacccaaaa attaaagtac tgtcggggtt tacttctaca gtcttcttta atgtctgaag
     2041 gtcccatata aggaagtgta aatactaaac tttttattga attggactca aaatttaagt
     2101 actcccatgt atgagcaaat tgattcctaa ttcttcttaa tagttcgatc tgttgaaatt
     2161 catcatcttg aagtaaacct agagcatgag ctaacaaaat tttagatgaa aaagttccta
     2221 ttggagcatt aaaaccatct aagagtttca aagagctttt accatcaatc aaaaatgctt
     2281 taataatttc ttccaaacga tcttccaaaa cagctgcagc acttaaggca gcacctcggt
     2341 cactctcttg attaaaatta ttaagaaaag ctcctaattg gaggatttct ggatgtgtat
     2401 cgttttttaa tttaggcatt gtttttaagc tccttaaagt atgttaattt attgattatt
     2461 aaacttatat ggaatcaaaa tgacaactga aatatttaaa gctggaactc aatacaatga
     2521 ctttacaggt acatctgccg cagaaactca aggattcgtt gaatatctaa aaagcaaaga
     2581 tctaataaaa gatgatgagt ttgtgattgg tatcgatttt tcccctttag tcggtatttc
     2641 aaaagaccag aatgaaatat ttctgcaagt ctatctaact aaagactttt cctatgatga
     2701 atatgaagca aaatcaagaa atgattctgt tttagaaatt cggaaaattg aaacgtatct
     2761 agatattgtt aaatttttct ctttgtttaa aagattaaat gtctctttct cttggcaagg
     2821 actattagaa aataaagaaa ttaaaataat taactagttg ttagttttcc taaaattaac
     2881 ataatacacc ttatgcgaag tcaaaaatgg gagcttaagc tcccattttt actgatgatt
     2941 ttttaattct ttgagcatag catcccgcaa aatttcattc attcgtgttt gatagccttt
     3001 gccttgagct ttaaaccatg caagtacatc agcatctaaa cgaatggaag tttgttgctt
     3061 cactgggcga tagaattgat tatggcgtac agcattgctc cagtcggtaa tttcaggcat
     3121 atctgagaga tctagctgat cgtcaggaac cgttccctta gcaagcaagc gttgaatttc
     3181 agcatcttgc ttgtcgctga atttttcatt cagttctttg cgtgagtatc taaccatgct
     3241 catatctatc tcgctccgct ttggtgactt gtcttgcact aatgattcgt atgatttcac
     3301 agtcatcttc atcaaagatg gtgtgagcta ctaagagcat tagtacgcct tttacttttc
     3361 caatggtttg ccaacgttct tcaccatcgg tatgtctgtc ttggattgag atccttaatg
     3421 gatcatcaaa aacaaggctt gttgtttcga aagagacatc gtgctttttc tgattctttc
     3481 gattctttgc ctcatcccat tcgaaatact gttcgataaa aaacattcaa atttgtatat
     3541 acaataatgt atcacaaaat tgtatcactc aagctgaaaa gcgtaatttt tagtcagatt
     3601 tattggcaag aactatcaaa aatgttcata aaaaagccga cttgtttcca agtcggtttt
     3661 ttaggttttg cgtcccaatt ttttatagca taaatttttg aaatatatag ctattttagg
     3721 atgcgtttcg tataagctct attatgttaa atggacataa atcaattcta aaattatcta
     3781 tgatcattct tacttttact tcagtactgc ggttagggtg gatagaaaag ggcctaactg
     3841 ctatcattgc ccccgaaacc ctccaaaata gatatctttc ttaatatgac acttacgcca
     3901 ccataagttt atttatctct taaaacagta aataactcat cactgtctag tgatgtggtg
     3961 tagtagcttg tgtgtcatat atcacatcac tagcctttcc taaaatttaa aaaaataggc
     4021 tattttcatg ttatccacta ttcgagaaca atggttctcc aatattcgcg gagatgttct
     4081 tgcgggtatt gttgttgctt tagctctaat tcctgaagca attgcctttt caattattgc
     4141 aggtgtcgat cctaaagtcg ggctatatgc ttcattctgt attgccgtgg taatttcttt
     4201 tgtaggcggt cgtccaggga tgatttctgc tgcaactggt gctatggctt tattaatggt
     4261 cacactggtt aaagagcatg gtctgcaata cttattagca gccacaattt tgacaggatt
     4321 tattcaaatc ctagcaggct atctaaaatt aggggccttg atgcggtttg tatcaaagtc
     4381 agtcgtgatt ggctttgtaa atgctttggc tattctaatt tttatggctc aattacctga
     4441 actcactaat gtgacatggc atgtctatgc gatgacaatt ggcggtttag ctattattta
     4501 tctattccct tatatcccag taatagggaa acttttaccc tctcccctca tctgtattgt
     4561 cctccttacg ctttttgcct tgttcattgg tttagatgta agaaccgtag gtgatatggg
     4621 gcaattacca gatacattac ctattttctt acttcctgat atccctttaa atcttgaaac
     4681 tttagtgatt attttaccct attcactagg tttggcagcc gtgggtttac ttgagtccat
     4741 gatgaccgca acaattgtcg acgatttaac agataccacc agtgataaaa accgagaatg
     4801 taaagggcaa ggtgttgcca atgtcgcttc aggcttctta ggcggaatgg caggctgtgc
     4861 catgattggt caatccatta ttaatgtgaa atctggtgga cgtacccgtt tatcatcatt
     4921 tagtgcaggg gtctttctgt taattatggt ggtgtttatc agtgactggc ttaaagccat
     4981 tccaatggct gcactggttg ctgtcatgat catggtagca attggaacat ttaactggga
     5041 ttcactacgc aatatccgcc aatatccctt atctagcaat attgtgatga tcgttacagt
     5101 catcgtggtc gttgcgacac ataatttggc ttatggcgta ttagttggtg tacttctttc
     5161 tgcattattt tttgccaaca aaattgaacg ctacatggca attcagtccg aatttaatga
     5221 acctgaaaat acccgtactt atacggtaac aggtcaagtg ttcttcagct cagcagataa
     5281 atttacttct gcatttgatt tcaaagaagc actttcaaaa gttgtgattg atgttagtca
     5341 ggctcacttt tgggatgtgt ctgcggtcgc tgccgtagat aaagtggtca ttaagtttag
     5401 acgtgaagga acagaagtcg aactgattgg aatgaatgaa gccagtaaaa cgttagtcga
     5461 taagtttggt atccatgata agccagatgc tttagatcgc ttaaatagcc actaagaggg
     5521 atgaattatg tcaaaaatta ttgcatgtat tgatggatca ttagcaacaa ataccgtttg
     5581 tgattatgca gcttggttta gcgataaatt gaattcacct cttcagttat tgcatgtcat
     5641 tgataagcca aaagcgaaag caccacaaga tttaagtggt gcaattggtc taggaagtcg
     5701 agaaaccttg ctaaacgagt tggttgaact ggaagaacgt aaggggaaaa ttgagctaga
     5761 gcatggccaa attcttcttc gggaagcaaa aaactatcta ttggagaagt tctctattga
     5821 tgctcaaagc tttcaacgcc atggcagtct tttagaaaca atcatgggaa tggaagagga
     5881 tattcgagtt cttgttatgg gtaagcatgg aactgaaacc gagcacaatt ccagtaaagt
     5941 tggaacccat attgaaaatg ttgttcgtgc tcttcataaa cccgtgttaa tcacatcctc
     6001 accgtttagt ccacctcagt catttttaat tgcttttgat ggaagtccta ctgcacgtat
     6061 ttgtgtagac aaaattgcaa atagaccttt attaaaaacc ctgacagctc atgtggtata
     6121 tgttggtaag ccaaatagtg atatgacatc acaagtcact tgggcaaaag agctacttgc
     6181 aaataatggt tttgatgtca ttgatagtgt gcttgaaggt gatgttgaga aatctatatt
     6241 ggattatcaa gaaaaaaacg ttattgatat gattgtgatt ggtgcatatg gtcattcaaa
     6301 gatacgccag ttttttattg gtagtacgac gaccaaattg ttatcaagtt ctacaaaacc
     6361 agtattatta ctaagataat ttttacaaga taaagggata gttaaaaact atcccttttt
     6421 tagaatttct aaaattaaca taatacacct tatacgaaat gcgttataat tatctataga
     6481 aatcaatgct gtagtttcta ttttatagcc cgaacatccc tgtacgggct tttttttgtg
     6541 atctttcacg taatatggct attctttaat caaatcctta caactttgat tagctcagtt
     6601 gaatagtacc cttagccatt cggcaagaaa aaccggctta accaccctct tttcggctgc
     6661 tccggttcaa ccggctccgg caccggaata cgcttatttt ccgttttttc cggagtagtc
     6721 aatccgtcat gtttgtcgtt atctacattc aatgtaggtt ctacttgaga tggactagat
     6781 tgcactgaag attgatctga agttggtgtg ggatgaacct gagttggctt catttcaatt
     6841 agggtaagta atgtatcaat acgagaattt gctttagctt cgcgatcttc tgctttttct
     6901 aactgtttct ttagaaaatc gatctgattc tttagttcaa taactgtatt gtctgtgtat
     6961 tcaggtgtat ttatagtttg tgttggagac gtatcagtgt gttttgagtg ttttccacta
     7021 aaaggttcac cataaacgcg gagtaactca gagaagtcga tagagccatc atgatcacga
     7081 cttaacaccc cattttttat atctttataa agtgtagcac gagatttctt atacagtgtg
     7141 gctgcatcac taacactgta ttttgtactt gttttcatag tgtattgctg tgtatttagg
     7201 atacgtattg aagaatacaa taagtgtatt cttcaataca ctcaagcttt aaaccctaag
     7261 ttctttaagt gtggcaaaaa ctgctgttgt tggtgagggt ttctaagcat atccttaatt
     7321 ttagcagcta aaatctcata actttcacct tctgaggcta aatgggaaag ctcatgtaaa
     7381 cgtgataatt gattcccgaa tagattaatt tgagcatcag atagctgata aaaatctttc
     7441 gaggtatgtg ttatggattt agatttttct ttaaaagaaa agataatatg agtgacttta
     7501 cgccctgttt ttttctgctc ataagtgact tttaaagggc tatattcatt gatttggtct
     7561 atagcagtgt caattactcg tttcttgaaa tcagcgaata atgggtactt tttaccgagt
     7621 tctaacattg tccgtaaact ctcaacagaa atttcacgct tacctatttg ccgatattga
     7681 accagtagct cataaatacg aataccgtat ccactattga tcccagcaat gtctgataag
     7741 gcatatttgg tgaattctct acttagattg gaaatgaatg gcagaatggg ttttgaaaaa
     7801 cggattccaa tgacactttg actatctaaa aattggattt cttgcaccca tctcattttt
     7861 atagatttat catcaatact gaggttaata gacctctcat agagtctatt aataccttcc
     7921 tttaaatctc tgtatgcagt tttctcatgt acgcccaatt gttttaattc atcgacgcta
     7981 acagggtata actcatcatc tgtaataggc tgatttttgg gaatccgtga aatggcagct
     8041 aagacaattc gttgctctac ggcacttaat tgatacgatg cttctattac ttgatttgat
     8101 tttacaacgt gatttttatt caaaacaaaa ccattgattt ttctaatata tgacaaatat
     8161 atacttgtca tagttagtag tcaatccgtc atagttaagt agtcaatccg tcatagttag
     8221 tagtcaatcc gtcatagtta gtagtcaatc cgtcatattt aaacgctgaa acctttgcta
     8281 tataaggcac agagacgact gaaaagattt aaaagattta aaatatatta aaaaggaaag
     8341 aatgaaaaat tgcccttggt tttcgctacg ctcaaactct attgaacttc gcttacgctc
     8401 agttcgaagg ggcaattttt tggttaatac catttggaat tcaataaaaa gcaagagcaa
     8461 ctcggaattc gcttcgctca ttagttttta tgctcgcttc gctcgaccta gatgcaaatt
     8521 aaatatgttc gcacccttcg ggatgctcta tacctcagtc tgagtagtaa agggaagctt
     8581 gtaatcaggg aaaaagcatg tcgaaaattt taattgggtc tgttcgctcg tagacactcg
     8641 ctttgtaagg catgaataaa gccagataat tttaaaaacc agaaccaccg taatcataat
     8701 catcagattt tggctttggc tgttcgggtt gaaccggttg agggggctta ttttgcatgg
     8761 gcaatggata atttggctgg gaatggactt caaactcaaa gcgtggcgtt gagttatgtt
     8821 ctgaatctct caattttcga ttcatgttat tcaggttcca gccttcttgg tgcagtatag
     8881 gctcttgaat tttgataatt tcactaaaat ctttttccag tttttcctta tttttcgcat
     8941 ttttgattaa gtaattcagg tgatacatca cttccaatga tgaaaatgga tgattttcag
     9001 cgctatagaa atattcatgt ttttgatcta attgcttttg gcgttcttct cgttttcgtt
     9061 cttgatgatt gtgttctcgg attttttgga tctgggcgag atattctggt gtcgcatttt
     9121 gttgacccgc acctagcttg ggctcagcaa gatacaaagt gctgtggatc tcattatatt
     9181 tttcatagct gtctgctgaa acctgatttt tgatggtgat gtcttggtca tccagtgtgt
     9241 aagtgactgt agggcagtgc tgtgcaaggg catcattcag atggttttcc cattgttttc
     9301 ggatgtagat caagaaagtg gaatagtttt catggccatc ttgaagttgg atttttttgg
     9361 caccgccttt ggctggatct tgagggttat agcgtttgaa gtactgttct gcgctgcgtt
     9421 caatgccgtc atccgctttt agcgagaaca tcaggtgaca atgaggctga tcttcgccac
     9481 tgatgcgtga ttttacgttg tgtatggcgt atgagtgcgg atattgctgt ggaactatat
     9541 gtttgtctaa aaacgtctct atgagcgttt tacgctgttc tggcgtgaat tcattcggca
     9601 gggcaatttc atactccatg tagaccttgg cattggctcg ttcatgacta tcggctgcct
     9661 gccaaaactc aatcggatct ttgacaaagg caggcatgca acgtccaggt tcaatatgtt
     9721 ctatggtttc attgcttgtt tttctaatgc cttcatattg catcagccgg gaaatataga
     9781 ggtagtggcc gatcgcatag cctttatttt tgctttttac actgaaatgg tagctggcca
     9841 tgttcacctc tggtggcttg gggaccggtt tttatgcagt gaaacggaat gaaaaccgtc
     9901 cggattttga aatgcctgaa tggctgagaa atccggttaa agggggtcaa gggggaatcg
     9961 ccccattgcg tactagtttc tccgattagg agaaactgtc aagtgcgaat tatttaaatt
    10021 aaaaataaaa tttcttgaaa agaattttat ttttaattta aataattatt ttcagactgc
    10081 cctctttaaa tatggtaaaa ccatatttaa agaggttgca gttctgaaaa ttcggcggca
    10141 gatgatatga ccctagaaaa agcaaaaaag cgtggtagac cttctcaact tttacaaatg
    10201 gcagagttac atgctttcgt tgagttttta ttagaaaaag atcctcgttc ggaacttcaa
    10261 aatcaggtta ttgatgcact acaagctgaa gactttaact ttgaaatgtt gtctgaagct
    10321 cagcagattc tggtgaaaga agcgttaaaa ccgtaccgtg agcatttgaa acttcagttg
    10381 ttgtttgatg agttggtgat aactccagga aggactgagt atgaagaaaa attcctggag
    10441 ctgtaccaag attataaaaa agatgatcta gatccggctg aaatgaatat cctgaaaacg
    10501 atgtgtacgc gttatctgaa ttttaaagcg caaaagcttg aatacagtga cttggagctg
    10561 tatttgagtc agatccagaa aaagtatgcc gggaaaaagc gaaaggctga aaatcaacgt
    10621 aagtttgaag tgggtggtgg ggttttaagt ctttataaag taaaagatga tgaaaattta
    10681 gacagtaaaa agtttctaaa aagaatcgaa attgaccgtt ttcttgccgg gaaatttcgt
    10741 caatctcaac tttataaaaa ggtttgggca ctagagcgac caaatacaga aaagtatcag
    10801 ctactttttg aggttctcca tcagctgtct ggatatagcc taaaagggca gactttaacg
    10861 cttctcgatt tagtgattat agatgctcaa aaaagataaa aagtaggtct gaaaaagaat
    10921 gaattttttg actgtccgct ttttagctga cttttctatt ttggcaattg taaatttatg
    10981 cgaaatttat tattttaaaa ataaggtcgc ctaccgtact tacgcatgag tagaaaaggg
    11041 ataaaaaacc ataggtttaa tttttaaagc catatctaaa atctaatttt cttaagtatc
    11101 aactcgtttg gttcttagca tttaaacatg cgttgctaga agtcttatga caccaaagat
    11161 tctttgtata aagagtttag agttggaagt agagaaaatt atgtctattc aagaattcgc
    11221 atttcaatta tctaaaaaaa ttcaggaaca gcattctgta aagattagcc gttcacatat
    11281 ttatgaatta attgctttag atcaggacta taggacctat agctcttttg ttgctcaaaa
    11341 tcttttactt gatgctcaat atgatgattc tgaagagtat tatcagcacg aattggttga
    11401 tgctctaact ctagaaatat tggaaaatcc gcctgaaaca gattatctaa attatagtga
    11461 agatgatctt cattgggatg attatgaagg taatgttcta ttggagcaga ttagaaaaat
    11521 tattataaga cttaaggttt tacttaagtt agatgcctca gaagagtttt atttgtcgat
    11581 ttctaaaaca gtctatcgag aacttttatg gcttaattta gaagttatta acttcaatgc
    11641 tgtaagagag gaattaagtt atatcaattt tgaaaatggt aaggtggatg acctagaact
    11701 tggaggtgat tatttcgagt tttcaaaaat tggggctatt tttgagaaga ttttatctta
    11761 tgcgcaagat cgtgagaatg ctgatgctta tgctctactc ggggcctact atcgttattt
    11821 agcaaatcag atagcccctt atggtcgaca tggctcaaat tttggaagtc gttgggacaa
    11881 taataaacaa aaatatatca attctgatga gactaagaaa aataaagaaa agtatgaaga
    11941 atacataaag caagcggaac actttgaatc ttatattaaa aattcaccta tgaatcttca
    12001 tgaaattaat ttggatgcag acaaggaaac agtttacaac cagattctgt acttgtgtaa
    12061 tcgaggtgat acgagtgcta ttgaatactt cctgtatgaa gaaattttca aaaatcatgg
    12121 agaagcatgg ttatacatct acttagctca aatgtgtgga gttgatttca cgcaggatga
    12181 ttttagagac tacaatgctt atacaggtga ggattatgat gactatggac ctattgaaat
    12241 cgcaggtcgt gaggcaattc aatatgctat agatttatca gaactggatg ttgataaaga
    12301 taatttagct aggaagattg ctgcagagtt atttgagaaa atttaattca tagtcttata
    12361 aaaagagaat ttgaaatctg tatttgactc ataaaataca gatttcatta tcatcgttta
    12421 acatcctggg ataagttgta gaaaggcttc tctaacttca gaaaattcgg cttgaggatg
    12481 atcttgtaaa aaatattcga tccaaatgaa tgttgcctca tggccgtcat aggaaggatt
    12541 tacatccaga taaaaagcca atcgtgctgt tatacaaagt tcaagatcgt taattaatgc
    12601 caatccaatc ttttttgctt gagaagaaat aagaagcttg ggctgtacga aatggttcat
    12661 aaaaacaagt cctatcaaaa gagtaaaatt gcttgataag actatttaaa cacataaata
    12721 atgtgttaaa cacattattt atgtgtttaa atgtaaataa atgatctgtg ctatactttt
    12781 ccatcagaac aattctg
//
