LOCUS       NZ_CP061555             3857 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS78 plasmid pAS78-4, complete
            sequence.
ACCESSION   NZ_CP061555
VERSION     NZ_CP061555.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092245
            Assembly: GCF_014718155.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:02:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,852
            CDSs (total)                      :: 3,756
            Genes (coding)                    :: 3,472
            CDSs (with protein)               :: 3,472
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 230 of 284
            Pseudo Genes (incomplete)         :: 50 of 284
            Pseudo Genes (internal stop)      :: 26 of 284
            Pseudo Genes (multiple problems)  :: 21 of 284
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS78"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS78-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            complement(join(3419..3857,1..1244))
                     /locus_tag="IC798_RS19090"
                     /old_locus_tag="IC798_19085"
     CDS             complement(join(3419..3857,1..1244))
                     /locus_tag="IC798_RS19090"
                     /old_locus_tag="IC798_19085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_257935357.1"
                     /translation="MQDFEQALFPGMDQSQYRVLWIEHQDKLNEETGERRLELNFLIPN
                     VEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQAVTTAKNLPKTVNEI
                     KETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIKNPNDDEKARPIRLT
                     GAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSAKRSTELEQQYRQHQ
                     NVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQTATSELAAVEQLEP
                     RNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHHRDGEQSPVVKKPRG
                     EPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTVIADYRAATAAAQRA
                     TEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQYHQETTESEAIRGFI
                     ARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADSTIERTASKETRLSA
                     TVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1460..1609)
                     /gene="mobC"
                     /locus_tag="IC798_RS19410"
     CDS             complement(1460..1609)
                     /gene="mobC"
                     /locus_tag="IC798_RS19410"
                     /inference="COORDINATES: protein motif:HMM:NF017524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_223861193.1"
                     /translation="MLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLK
                     ELKK"
                     /name="mobC"
     gene            complement(1621..1803)
                     /locus_tag="IC798_RS19415"
     CDS             complement(1621..1803)
                     /locus_tag="IC798_RS19415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223861277.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLAQIDAT
                     QGNAKKRIRKTTTQN"
                     /name="hypothetical protein"
     gene            complement(2171..3133)
                     /locus_tag="IC798_RS19100"
                     /old_locus_tag="IC798_19095"
     CDS             complement(2171..3133)
                     /locus_tag="IC798_RS19100"
                     /old_locus_tag="IC798_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     Typing          complement(4..1463)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.509"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgttctgtgc ctgaatgagc tgcaaggctt gctcttgctg cttctgttgc tctttgagct
       61 gctgctgttg ctgctcgata atctgcaata actgtgcttc gagattcatt taattggttt
      121 cctgcactat tcgcatgttg ttgctgtact gctgcgcttt cgggtcgatc tggacgcacc
      181 agttccattt tttctctgtc ccacacttct gaatattcag gctcgcccct tggcttttta
      241 accactggac tttgttctcc atctctgtga tgtcgctgta tttgctcttg ctgacgtacc
      301 ccagacaaat actgctgata agcgttatac atgccagtga aatcaaggct atattcgatg
      361 taataagggt tttctttaat ttggctgctt gggctgtttc ttggctcaag ctgttcaact
      421 gctgcaagtt cgcttgtagc tgtttgatct gctgttgttg ttcttgcaat agggctgtca
      481 tgccttgttg ctgctgcttc ttggtttgag ctgtagtctg gtcgatctgc tgcaatatct
      541 cgttcgttgc ttcgctctgt ttctgctgta tatacgtttt ggtgctgtct atactgctgc
      601 tcaagctctg tgcttcgctt tgcactgtat tccgcatatc tttgaatatc tgactgattt
      661 cgctgtttag cttctcgtct atatcgttct gatgctgcga tagtaagttg ttggctcgtt
      721 tctgtaagtc taaaatcttg ctcatagatt gcacctgtaa gccttatcgg gcgggctttt
      781 tcgtcatcat tcggattttt aatggatagg cttttcttgg tttctctggt gacttcaaga
      841 ccaagatcca atagccattt tttcatgctc tgccgatctg taattagtcc accttcaacg
      901 gctctcgttg cttcaatatc aagcgtttcc ttgatttcat taacagtttt aggcaagttt
      961 tttgccgttg taacggcttg gcgatagagg gggtcatctg catcatgcag ttgatattca
     1021 tagtttgtaa tcttcttaaa cagatcaata cgaggacgat cagctttatc ataataaggc
     1081 tgtaaacgtt gccctgtaag gatttcaaca ttagggatta ggaagttaag ctctaatctt
     1141 cgctcccctg tttcttcgtt taatttgtct tgatgctcaa tccacaagac acggtactgg
     1201 ctctgatcca tgccagggaa taaggcttgc tcaaaatcct gcatgatttt ttgtttatcc
     1261 tggtcagata aatcatgctc ataaaaactt aggcagccgg atgtatattt ttttgcataa
     1321 ggtgacgtat caattaatcc tgatacttcg gaaacatcgc cctgtaggac ttttgcataa
     1381 tctcgatcac ggtctttacc aagtaaataa tccagtggac ctttacttaa accgttccca
     1441 tatctaaaga attgaacaat cattttttca attcctttaa ggtctgatca atccctatta
     1501 acaggtgcaa aagccttgca gcatcaaggg gacgactgct tgcaatatca gtatttattg
     1561 cctttgcaat ctgattgatg ttgcccccaa ttcgggatag ctcaagcaaa aagtcacggt
     1621 ctaattttgt gtagtggttt tcctgattct ttttttggcg ttgccttggg ttgcatcaat
     1681 ttgggcaagc gaagtatccc ttagatattt ggcaatattt aggatattgg catctttgat
     1741 tttctgtacc tctgcatcag aaaatcggac tttgtaaacc ttattacgtt tgatcgctgt
     1801 catgcttttg cctttgcttt tggggggttt cgggggcttg cccctgaaca aggtcatgga
     1861 acacatctgc gtagcagagg ggtctccatg tatccttgcg tttcattacg atagcagacc
     1921 cctagacgta attcaattaa gattaagcag gtcatagcaa gactatggac aatcgcaagc
     1981 gaccgtaaaa cctgatctgt gcctaaacac tatgcacaac tccgcataac gataaagccg
     2041 ttatacgttg agttgcacac aggtttagat cacagggttt tactttgccc acagccgttg
     2101 ctcattccct ttaataataa tttttaaaaa tgaaatggga aaaaagcacc ctagggggct
     2161 gatatctgat ttaggcttgc actttccgct tttttatcca cgttctgatt gttctatccg
     2221 aaacattcaa ttgtagggct atatctttct gctttatgcc ctgtatatgc agttttaagg
     2281 cttcctgacg catatcactg tattgctgtg acctagcctt accgccatga ctggaatcac
     2341 tgcttagacc gcctttacgc cctttgacag tctgacgctc gataaatttg gcatgtgctt
     2401 ctgtgtgatt gcgccatacc caacgagcaa tagacttagc ggttgccttt acctctgaat
     2461 atggcaatgg ctctaaaaac gcaccatttg cgtttatagc gacttttagc acttccgcat
     2521 accaactatc aggcgacccc gaaataaacg ctctaatcgc ttcatacgcc caaaaacgaa
     2581 cggtatcgaa catcatacag ttacgcccaa gccctgaaac ttcttttaag tcggttttta
     2641 gttcctgaag ctctacaaaa tcggctaaat aatttaggtc gtatggttga acgccaaagg
     2701 tatgattttc ccatgttccg tgacatggat ttttagtgat taaccccgta tagccacgat
     2761 ctgcacctaa cgccaagcta tacgcctgct caatcttggc aaggtactgt atgacattct
     2821 gctttgctcg atgcgtggta cacactggag ctaaaagcat atagccaata tgtgcatgtc
     2881 cattttggcg atttttggct atccatgttg gggtcggtaa atttgcgtca gaccacgcaa
     2941 aataagcgtc ttggcggtct atatcgaaca ccaacgagct aacaagacaa gggggattat
     3001 gctgaatgta tggcttctgg attgcggtac gttttgggcg aataatcacc ccacgcccta
     3061 aatcatccga gcaatacggt ttttttggga gtttttcgta aaaatcgtct gaaaattgcg
     3121 ttttattgag catgaaacac gtccaagtgt tgcattggct caaaacatca gtaaaataca
     3181 agtatcgaat ttgagtttta cggatgcttt aagcgtttgt ttaacaaaga gaaaaaagcg
     3241 ggtcgccaaa cctgcttttt tttcgcctga tgaaaatatt acaccttttt gaattgctgt
     3301 caaatgactg attttcaaaa caaaatattg cgacaaaatg tcgcaaatat gtgacctttg
     3361 aagatgcttt ttttcgagga cagcattgca agggcaatgc tccgcagaaa aaaaagtatc
     3421 aaaatctagg tgaataactc ctgtcatttt tacgttgttg ctccagttgc aactctctac
     3481 gtctgtccag ttcatctaaa gccttgaaga tatttgtttg atcaaaccct cgaatttttc
     3541 gtgatactgt tgcactgagt ctagtttcct ttgaagctgt tcgttcaatg gtgctgtctg
     3601 cttctcgatt tctgtataga tcatcgttgg caatagttcg ttgatctcgt tctttagatt
     3661 taacccagtc actaattgct ctaaatggct ctgtaatcgc tgttttaagc tgctctccaa
     3721 gccttgcaat aaatcctctg atagcttcgg attctgtagt ttcttgatga tattgatcag
     3781 ctcgttttct ttctcgttca actggttgca gtgcttcact agtttgctgt tgtagttctc
     3841 taattcgtct atggttc
//
