LOCUS       NZ_CP061554             5891 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS78 plasmid pAS78-3, complete
            sequence.
ACCESSION   NZ_CP061554
VERSION     NZ_CP061554.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092245
            Assembly: GCF_014718155.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:02:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,852
            CDSs (total)                      :: 3,756
            Genes (coding)                    :: 3,472
            CDSs (with protein)               :: 3,472
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 230 of 284
            Pseudo Genes (incomplete)         :: 50 of 284
            Pseudo Genes (internal stop)      :: 26 of 284
            Pseudo Genes (multiple problems)  :: 21 of 284
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061554.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS78"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS78-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            complement(join(5317..5891,1..1285))
                     /locus_tag="IC798_RS19060"
                     /old_locus_tag="IC798_19055"
     CDS             complement(join(5317..5891,1..1285))
                     /locus_tag="IC798_RS19060"
                     /old_locus_tag="IC798_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017386457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_017386457.1"
                     /translation="MISDFVNGFFNSGVGKYIICATFSFIPVFLSGSIVVKFAHATARK
                     RIENGNTEAQSFLQYWFWFFVSMALIITAFVTLTNLSLDWMLKVYFFWGLPAIFYMLYW
                     LRSLTIRDDLYFFLSKEEKLEYNNQNNKVESNIEKRIDIENRAGLFEKLTITEEPTAPL
                     LWLGDKLEDDLWSENRFKARVSNEDRGMVVGAPGSGKTAFLIAQVVDWMQSGRSVVLTD
                     VKPEIYGRLLEAGVFERYGYTPTVFNPTDIHSHAYNLFSESSSSAELNEILSVIIPYVD
                     DSSEVFAENARRLLKAVLLELGEEASLPNAQKFINSEDNIADLLKILKRSSNDSVSAIA
                     SEIGRTAKNENLLASIFTSLSKAFAFLDDDRIRKQVQRSDFSLKKLLVQPKQILFLQFE
                     QEFRSSTATIFGAMVAQTLRILQTNCRNRDAVFVALDEIINCAPIPNFTSTLNMIRSAK
                     MPTFLYFQSLEGLNRLYGNDAERLIMGSADLKVVFRIGDDETCQTFSRMVGETEALIGG
                     FAEVKGFTSTPNIHNPAILLNNDIVSNSGSKTLSKNTGILKVFEPEKFQQLEQHTAICL
                     YKGRSALSLMPCFYEDYGIKEEHWPREPAFATLGEIAESKKIA"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            complement(1282..1521)
                     /locus_tag="IC798_RS19065"
                     /old_locus_tag="IC798_19060"
     CDS             complement(1282..1521)
                     /locus_tag="IC798_RS19065"
                     /old_locus_tag="IC798_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017386456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017386456.1"
                     /translation="MGIASEIRGNLPLCGRCINCKLLVWEEKAELKTSSNLIKFKSLDS
                     AYYYMVRCSWLKSPVNEPQFLEICEGKQEYGAKG"
                     /name="hypothetical protein"
     gene            complement(1521..2411)
                     /locus_tag="IC798_RS19070"
                     /old_locus_tag="IC798_19065"
     CDS             complement(1521..2411)
                     /locus_tag="IC798_RS19070"
                     /old_locus_tag="IC798_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="WP_017401723.1"
                     /translation="MNRLEYKLMQICDRNKDGSHTTQANRRAMLSQMTEDLKSLGYKVK
                     HMQPSDLKGRHINALVKKWKEDGKSIGTIKNRMAVVRWWSEKIGKADMVKDNSNYGIEN
                     RVYVTNTDKSKTLKDIDLSGVSPYISQSLRLQDAFGLRREEAMKFQPEYALDGQSIDTA
                     KYIRLKESWAKGGRARTIPVTSEKQRQELKSALALAKDGSLIPPEKTYKAHLATWEKVT
                     NALEIGQTHGLRHGYAQTRYLELMGFPCPAVGGYRELTEAEKVKDGEVRMQISEELGHS
                     RINITSVYLGSWSKK"
                     /name="phage integrase N-terminal domain-containing
                     protein"
     gene            complement(2500..2727)
                     /locus_tag="IC798_RS19075"
                     /old_locus_tag="IC798_19070"
     CDS             complement(2500..2727)
                     /locus_tag="IC798_RS19075"
                     /old_locus_tag="IC798_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017386460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017386460.1"
                     /translation="MNYELHIPTKTVKLGQQKIQVSSADQVHFDGYQIGSQRFFLYHVD
                     PLTKKETITTIPPKLAQDLINKLHLFAVFA"
                     /name="hypothetical protein"
     gene            complement(3084..4067)
                     /locus_tag="IC798_RS19080"
                     /old_locus_tag="IC798_19075"
     CDS             complement(3084..4067)
                     /locus_tag="IC798_RS19080"
                     /old_locus_tag="IC798_19075"
                     /inference="COORDINATES: protein motif:HMM:NF013603.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_307878558.1"
                     /translation="MKHGKKTNWGNLQRCGSVWSCPVCAKQITEERRVEMGQLIKYWEQ
                     QENSDIKLMTLTFSHSKDEPLKVLLRKLKNAITMFFGGRQFKDLSKLCKIQYKVRGLEV
                     TYGSNGWHPHFHILLLTKDTDDLAYAYRDRLAKYWMHCCESKGLKSPSMKHGLDIRDGS
                     YAQEYVAKWGLDYEMTKGHVKRGRKESLSPFDLLQLSIEDQEIFEKLPSKLWQEFAISM
                     KGARQLVWSRGLKKLVGIGEKSDEEIANETDEESITLREVDQITFDLLIKYKKRHEFLE
                     WQKGDFLNGCYGSGETEQNLIALFERYIAELPDGQSEFIHACKSLV"
                     /name="protein rep"
     gene            4726..5124
                     /locus_tag="IC798_RS19085"
                     /old_locus_tag="IC798_19080"
     CDS             4726..5124
                     /locus_tag="IC798_RS19085"
                     /old_locus_tag="IC798_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017386458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_017386458.1"
                     /translation="MARITVTQASKDFNISRNTLYKKIKQGHLTKDSNGLLDVNDLIRV
                     IGAHTESTSKNVTLNTSDIQSVSSDTQLQQAKSEVEQLKQLLAQKEIFINQLQQQIADL
                     RQDKEQLYNQINQKRLEHKKGLLGRFFG"
                     /name="plasmid replication DNA-binding protein"
ORIGIN
        1 cacgattacg acaattggtt tgtaaaattc gtaaagtttg agcaaccatc gcaccgaata
       61 ttgttgctgt tgaactccta aattcctgct cgaactgtaa aaaaagtatt tgttttggct
      121 gaacaagtag tttttttaat gaaaaatcag agcgttgtac ttgttttcta attctatcgt
      181 catctaagaa agcaaacgct ttagataatg atgtaaaaat agaagcgaga aggttttcat
      241 ttttcgcagt acgtccaatc tcagatgcaa ttgctgatac tgaatcatta cttgatcttt
      301 tcagaatttt taaaaggtca gcaatattat cttcggagtt aatgaatttt tgagcatttg
      361 gaagtgaagc ttcttcgcca agctcaagca ataccgcctt taaaagccgt ctagcatttt
      421 ctgcaaatac ttctgaactg tcatcaacgt aagggatgat tacactcaaa atttcattta
      481 attctgcact actagatgat tctgaaaaaa gattgtaagc gtgagaatgt atatcggttg
      541 gattgaaaac cgtaggagtg tatccgtagc gttcaaaaac accggcttcg agtagtcgtc
      601 cataaatttc cggcttcaca tctgttaaga caacagatct ccctgattgc atccagtcaa
      661 caacttgagc aatgaggaaa gcagttttac ccgaaccagg tgcaccaact accatacctc
      721 tatcttcatt ggaaacacga gctttaaaac gattttcaga ccataaatca tcttccaatt
      781 tatcgcctag ccaaagcaaa ggtgctgttg gctcttcagt aattgtaagc ttttcaaaaa
      841 gcccagcacg gttttcaata tcaatacgtt tttcaatatt ggattcaact ttattatttt
      901 ggttgttgta ttccaacttt tcttcttttg aaagaaagaa gtaaaggtca tctcgaattg
      961 taaggcttct caaccaatac aacatataaa atattgcagg taagccccaa aaaaagtaga
     1021 ccttcaacat ccaatctaag gataaattag tcagcgtaac gaatgcagtt atgataagag
     1081 ccatgctgac aaagaaccaa aaccagtact gcaaaaatga ctgagcctca gtattaccat
     1141 tttcaatacg ttttctagcg gtagcatgag caaacttaac aacaattgaa ccacttaaaa
     1201 aaacaggtat gaatgaaaaa gtagcacaaa taatatattt accaacaccg ctattaaaaa
     1261 agccattcac aaaatcgcta atcatccttt tgctccatat tcttgttttc cttcgcaaat
     1321 ttccaagaat tgtggctcat taacaggact ttttaaccaa gaacaacgaa ccatataata
     1381 ataagctgaa tccaaagact taaactttat caagtttgaa cttgttttta attctgcttt
     1441 ttcttcccaa accaacaact tacagttaat acaacgacca caaagcggca gattcccacg
     1501 tatctcggat gcaataccca ttatttttta ctccatgacc caaggtacac ggacgtaata
     1561 tttatacggc tatgccctaa ttcctcacta atctgcatac gcacctcacc atccttaacc
     1621 ttttctgcct ctgtcagctc tcgataacca ccaacagcag ggcaaggaaa gcccattaat
     1681 tctaaatatc gtgtttgagc atagccatgt cttaaaccat gtgtttgacc gatctcaagg
     1741 gcattagtga ccttttccca tgtggctaaa tgtgccttat acgttttttc tggcggtatt
     1801 aacgaaccat cctttgctaa agccagtgca ctcttcagct cttgtcgttg cttttcgctg
     1861 gtaactggaa tcgttctagc cctaccacct ttcgcccacg attctttcaa ccgtatatac
     1921 ttggctgtat caatcgactg cccatcaagt gcatattcag gctgaaattt catagcttct
     1981 tcacgtctaa ggccaaaggc atcttgtaat cgcaaagact gactgatgta aggtgaaaca
     2041 ccacttaaat caatatcttt taaagtcttt gatttatcag tattagttac atagacacgg
     2101 ttctctattc cataattgct gttatctttc accatatctg ccttaccaat cttttcagac
     2161 caccagcgga caacagccat tcggtttttt atcgttccaa tactttttcc atcctctttc
     2221 cactttttga ccaaggcatt gatatgtcta ccttttagat cactaggttg catatgctta
     2281 actttatagc cgagactttt taagtcctct gtcatctgtg acaacatcgc tctacgatta
     2341 gcttgcgttg tatgtgatcc atccttattt cgatcacaaa tttgcatcag cttatactct
     2401 aacctattca tttttataac cttaaaaaag aacccctttt tatgcgagga caaatgtcct
     2461 tttggacatt taccacagca taaaaaaggg gtaaaaaaat taggcaaata cagcaaataa
     2521 atgcaattta ttaattaaat cttgagccag ttttggtggg atagtcgtaa tcgtttcttt
     2581 tttcgttaat ggatcaacgt gatataaaaa aaagcgctgt gaaccgattt gataaccatc
     2641 gaaatgtact tggtcagcag atgaaacttg tattttttgc tggcccaact tcacagtttt
     2701 agtcgggata tgtaattcat aattcataag gagtcagcct tggtaagtgt taatgctcat
     2761 cttgtatcgg taacgaacaa aatttaatag agcagataga tacaacagtt gcaaaagcaa
     2821 taattaagcg tgtaagacgt taatgtttaa tgtgtgtatg gactcctatt tttgagtttt
     2881 atttaagctg tgaaatcaaa agaaaaaaca gcaagaaagg tcatgaaaag acaaaataaa
     2941 gaacctaaat tatagcctat tttttatgct tgtaaacaca atcacgcagt aataatttaa
     3001 aaaaactaca tctaatctat agatagccta agtttgcgtg gtggtcacta ccgataaatc
     3061 ggcagttacc accacgcccc acgctacacc agcgatttgc aagcgtgaat aaattcactt
     3121 tgcccatcgg gtagctctgc aatgtatcgt tcaaaaagtg ctattaaatt ttgttcagtt
     3181 tctccgctgc catagcagcc gtttaagaaa tccccttttt gccactccaa gaactcatgg
     3241 cgttttttat acttaattaa aagatcaaaa gtgatctgat caacttctct taaagtgata
     3301 ctttcttcat ctgtttcatt tgcaatttct tcatctgact tctcaccaat cccaaccagt
     3361 tttttgagac ctcgagacca aaccaattga cgagcaccct tcatagaaat agcgaattcc
     3421 tgccataact tacttggcaa tttctcaaaa atttcctgat cttcaatcga caactgaagc
     3481 aaatcaaacg gacttaatga ttctttacga ccacgtttta cgtgaccttt tgtcatctcg
     3541 taatccagcc cccatttcgc aacatattct tgagcatacg aaccatcccg aatatccagt
     3601 ccatgcttca tagatgggct tttcaatcct ttcgactcac aacaatgcat ccagtatttt
     3661 gcgagtctgt ccctatacgc ataggctaaa tcgtcagtat ctttggtcaa taaaagaata
     3721 tgaaaatgcg gatgccatcc gtttgaacca taagtgactt caagaccacg gactttgtat
     3781 tgaatcttgc aaagcttgga taaatctttg aactgccgac caccaaaaaa catagtgatc
     3841 gcatttttta acttgcgtaa aaggactttc aacggctcat cttttgaatg actaaatgtc
     3901 aaagtcatta atttaatatc tgaattttct tgttgttccc aatatttaat taactggccc
     3961 atctcaacac gtcgttcttc agtaatctgc ttagcacaaa ctgggcatga ccagaccgat
     4021 ccacagcgtt gcaaattgcc ccagttagtt ttttttccgt gcttcattaa acatgacagt
     4081 acggttttta tgtttatcga tacgtctttt taaacaatta caaacacgtt cttgcggcaa
     4141 aagttttgca gattgatctt gaagaatata gctgtgaagt ctagccccta caccctgttg
     4201 ttattttttg tacagtaagg aggctgaaag cctttgtttt cgggcgtggc ctgagattta
     4261 gtaagtttac caagaccacc cgattttgat gcagacgagc cttcagaaga cgcaggaacg
     4321 cctttaacaa aaagtgtggt tttcgtgggt tttgtgaaat ttactgtaag atttacacta
     4381 tttggcatct ctcaagtcct tttaatgact tgcgagatgt ttgcttactg ttataataac
     4441 tttgcatgct gacattcgct gacggatcag tgtgtacatc aagagcccat ctagtcgcca
     4501 aactgaatac gggctttttt tgtatctgca ccaatcatat tgttaataaa aattctgtta
     4561 tgaagttaca actgtttcat aaacattgca atactgctgc cactttagct taccaaaatt
     4621 ataattttta aagccttata cataaaaaac ttcggaaaaa aggtttatac cttccatacc
     4681 tccatgtaac ctgcaattac gtcactataa acattggatg acactatggc gcgtataaca
     4741 gttacacaag catcaaaaga ctttaatatc agtcgaaaca ctttgtataa gaagataaaa
     4801 caaggacatt tgacaaaaga ttcgaatgga ttacttgacg ttaatgactt aattcgtgtc
     4861 atcggtgcac acacagaaag tacatctaaa aacgtcactt taaacactag tgacattcaa
     4921 tctgttagca gtgacacaca gctacaacag gcgaaaagtg aagttgaaca acttaaacaa
     4981 ttactagctc aaaaagaaat tttcataaac cagctacagc agcaaattgc agacctacgc
     5041 caagacaaag aacaactcta caaccagatt aaccaaaagc ggctagaaca taaaaagggg
     5101 ttacttggca ggttttttgg atagtttacc tgttataaaa agcagtaatc agagaaggga
     5161 attacccgaa tatctcttgt cgctgggtag acacccagct taagccgcca agactggcgg
     5221 ttttatctag agttcaggga cgtgaccagt gaaaaacaac aagtccgctc tgttagcgga
     5281 cttgtggcac agcaaagctg cgccgatcat gcagacttag gctatctttt tgctttctgc
     5341 tatctcacct agtgttgcga atgctggttc tcttggccag tgttcttctt taattccata
     5401 atcctcataa aaacacggca ttaatgatag ggctgatctt cccttataca agcaaattgc
     5461 tgtgtgttgc tccaattgtt ggaatttttc aggttcaaag accttaagaa tgcctgtgtt
     5521 tttacttaaa gtctttgatc cactgttact tacaatgtcg ttattcaaca aaatcgcagg
     5581 gttgtgaatg tttggcgtgc ttgtaaaacc cttaacttca gcaaaaccac caattaaagc
     5641 ttcagtttca ccaaccattc ttgagaatgt ttggcaagtt tcatcatcac ctattctaaa
     5701 gacaacctta aggtctgctg atcccattat cagacgttcc gcatcattcc cataaagtct
     5761 atttaagccc tctaaacttt ggaaatagag gaaggtaggc atttttgcag aacgaatcat
     5821 atttaatgta cttgtgaaat tagggattgg tgcacagtta ataatttcat ctagcgcaac
     5881 aaaaaccgca t
//
