LOCUS       NZ_CP061553             8101 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS78 plasmid pAS78-2, complete
            sequence.
ACCESSION   NZ_CP061553
VERSION     NZ_CP061553.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092245
            Assembly: GCF_014718155.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:02:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,852
            CDSs (total)                      :: 3,756
            Genes (coding)                    :: 3,472
            CDSs (with protein)               :: 3,472
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 230 of 284
            Pseudo Genes (incomplete)         :: 50 of 284
            Pseudo Genes (internal stop)      :: 26 of 284
            Pseudo Genes (multiple problems)  :: 21 of 284
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061553.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS78"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS78-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            336..527
                     /locus_tag="IC798_RS19010"
                     /old_locus_tag="IC798_19005"
     CDS             336..527
                     /locus_tag="IC798_RS19010"
                     /old_locus_tag="IC798_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102617.1"
                     /translation="MSTELVNSASIISFPKSCGICESKENVQMFAGLMLCRHCQENIRI
                     TNPGLFAANEIIEPKAQD"
                     /name="hypothetical protein"
     gene            580..783
                     /locus_tag="IC798_RS19015"
                     /old_locus_tag="IC798_19010"
     CDS             580..783
                     /locus_tag="IC798_RS19015"
                     /old_locus_tag="IC798_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191108097.1"
                     /translation="MYDREKLLNAFKSAKGIVDHEFSQDIIDVCHVKDQARQQRILEGE
                     TTFEEEDAALMAEWRKLNNSSC"
                     /name="hypothetical protein"
     gene            1017..1523
                     /locus_tag="IC798_RS19020"
                     /old_locus_tag="IC798_19015"
     CDS             1017..1523
                     /locus_tag="IC798_RS19020"
                     /old_locus_tag="IC798_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191108098.1"
                     /translation="MKNFDLQINKITAVKNPIYYGDDFLHLCIYTEQASYIQFFCLDLF
                     CYVNNINELLACILHKETRADYSFIEWIVNTEFKEISIQELKIQINNLINQLDIISKEL
                     PTNLSAKLLKEYLCTLNFENITNSFEILGNHKVVPNNPDNGGPNYQFFWENSEKIYFLH
                     IHLES"
                     /name="hypothetical protein"
     gene            complement(2019..2561)
                     /locus_tag="IC798_RS19025"
                     /old_locus_tag="IC798_19020"
     CDS             complement(2019..2561)
                     /locus_tag="IC798_RS19025"
                     /old_locus_tag="IC798_19020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191108099.1"
                     /translation="MKIIVILSTLLMLSGCATLYKLDDAITDPGKRYQEPSTGKTAQVR
                     VIYNIGADVSIYPNSLVRKDMLKDPDGGAAITKRNRAGLMKVTYQPKSLGMPDTPKDFS
                     NFGEFKVPADKFILVRMSYSNVDNIRSISCPLKVFKVKFEENKNYTLNMGVGSRCHYQL
                     YENKDNKFIDIKQIEEI"
                     /name="hypothetical protein"
     gene            2810..4102
                     /locus_tag="IC798_RS19030"
                     /old_locus_tag="IC798_19025"
     CDS             2810..4102
                     /locus_tag="IC798_RS19030"
                     /old_locus_tag="IC798_19025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_191108100.1"
                     /translation="MATGISKNAFDEASTLALLRHKNPYPTVPNTPIEISYKNKLAEFE
                     ILKPLISEFIDVGGNKLKVNIEGSDLVLGDNIFALHQLIKQNTKVDLIYLDPPYNTGMD
                     FESRSQEHAYNDSFAIASYLEDMRIRLILMREILSDQGSLYLHIGHQMMPYLKVILDEV
                     FGVKNCRNIITRRKCSSKNSTKNQYANLNDYILFYTKTSNYIWNPQGEKPSDEWIQKEY
                     PKVDSKGQYKLVPIHAPGKRNGSTGTEWRGKMPPEGKHWQYTPEKLDALDEAGEIHWSK
                     TGNPRRKVYLTNDKKLPYNDYWADFRDAHHQSVKITGYPTEKNLHMLKMIVAASSNTNS
                     LVLDPFCGSGTTLQASAELGRQWIGMDQSFIAMKYSINRLTKGTKEMGDYVLSKQTSQL
                     DLIESENLHNFRLLVDKDIYIEYQDQVDNLL"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(4097..4972)
                     /locus_tag="IC798_RS19035"
                     /old_locus_tag="IC798_19030"
     CDS             complement(4097..4972)
                     /locus_tag="IC798_RS19035"
                     /old_locus_tag="IC798_19030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191108092.1"
                     /translation="MGIINKYQHDPYEIITVLSNGANFSLDSEIALKKYEKPIRGFIGL
                     VNLSTSSADLLGKIRVSSIDKDTRMSYLKLFRRCVCSSLDTELSKKITKVSTQDLVNTF
                     QDYFADIANLKHFFNNVFDNTHLLSLACLLAEYDNRGSSGYELTDLVFDELERLYPHTE
                     ILGPRGAGKDIELNTVYPSFKGGKFPCDFVIKDTNTQKVLAVGFCRYDSTRGGAQSDDR
                     TGGNSQKVSQLKDHYSSTGEVIKVLFVSDGPGLAHKDTWEETLSLDDSWDDNVRVTTLK
                     TLEEVTLNWL"
                     /name="hypothetical protein"
     gene            complement(5019..5324)
                     /locus_tag="IC798_RS19040"
                     /old_locus_tag="IC798_19035"
     CDS             complement(5019..5324)
                     /locus_tag="IC798_RS19040"
                     /old_locus_tag="IC798_19035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191108093.1"
                     /translation="MAILFLDSKTIGVNFSDRYDHQSSLSHDFINPLINPTEVTHVSAF
                     IHTYAHQQFGVIQSGKFGIRFALTNSMSADWLYDRKEDMLHDFDRLKKTLCEGLTN"
                     /name="hypothetical protein"
     gene            complement(5344..5568)
                     /locus_tag="IC798_RS19045"
                     /old_locus_tag="IC798_19040"
     CDS             complement(5344..5568)
                     /locus_tag="IC798_RS19045"
                     /old_locus_tag="IC798_19040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191108094.1"
                     /translation="MKFSLLRHEELFLQQLDDLIVSTEDVDKKILLIDRKINFIKNLMD
                     FERDFLKHDLECMKQEDSYRLNIDKKRNS"
                     /name="hypothetical protein"
     gene            complement(5788..6330)
                     /locus_tag="IC798_RS19050"
                     /old_locus_tag="IC798_19045"
     CDS             complement(5788..6330)
                     /locus_tag="IC798_RS19050"
                     /old_locus_tag="IC798_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_191108095.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKNSDGLIDFSEALRV
                     FGEPQNKTSNVNQSQSTNSQNLTEVDLLKRQVDILEKQLNQALLRENQSLERESFYQEQ
                     IEAMQRLLEAPKPHIPTSVDPEPTQAEQIPKEKEEVITMSQKELDTNENKRIPIPEHVE
                     PEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6396..7319)
                     /gene="repM"
                     /locus_tag="IC798_RS19055"
                     /old_locus_tag="IC798_19050"
     CDS             complement(6396..7319)
                     /gene="repM"
                     /locus_tag="IC798_RS19055"
                     /old_locus_tag="IC798_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022088.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_191108096.1"
                     /translation="MKDLVVKDNALINASYNLDLVEQRLVLLAIIEARQTGKGINANDA
                     LVIHASSYIENFGVEKHTAYAVLKEASKVLFDRRFTYQSLTDKGNVKTTHSRWVSEISY
                     IDNEASVSLIFSPAVVPLITRLEQRFTSYELKQVSQLTSRYATRLYELLVAWRTTGQTP
                     VFEVSEFRQQLGIADDEYTRSDNFKRRVLDIAISQINTFTDIKVKSEQHKTGRSISGYS
                     FSFKQKTSGKNTSKTSNQQLKLIGQLTDKQILLFSGKLAYDPTFASKYSKTGESYDDFI
                     IRISGDLTDPKKIKEYSPYLKELGFK"
                     /name="repM"
     Typing          complement(6606..7118)
                     /name="rep_cluster_2359"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.097"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggtacatacc ggcaattttt ggttcacaca ccacgcatct aacgataaaa gcagaaattc
       61 ttttaaataa atggtttacg tcatttttta aataaagaaa ttgtgtacaa aagaatgagt
      121 gtttttgata ttaagttatt gatttatctt attataattc cgtacatggt gttgtacaaa
      181 atatcaggac ttttgagatc taaagatatg aaagttatag agaattattt gtacacctgg
      241 atcatacaag gcgtgtacag attttaggta ataaattaag tataaagtat tgaaacttat
      301 taaaaatcag tgtgtacatt ttaaggataa ttattatgtc aacagagttg gtcaattcgg
      361 caagtattat ttcttttcct aaatcatgtg gaatttgtga atcaaaggaa aatgttcaga
      421 tgtttgctgg actaatgctt tgtcgccact gtcaggagaa tattaggatt actaatcctg
      481 gtctgtttgc tgctaatgaa ataattgaac ctaaagccca ggattgattc tgattcgatc
      541 aagcttgggg gttttgaata aaaaaggaga tctactgata tgtatgacag agagaagcta
      601 cttaatgctt ttaaaagtgc taaagggatt gttgatcatg agttttccca ggacattatt
      661 gatgtctgtc atgttaaaga tcaggctaga cagcagcgta ttttagaggg tgaaacgact
      721 tttgaggaag aagatgctgc tttaatggct gaatggagaa aattgaataa ttcttcgtgt
      781 tgagttctgt ttttttatca aaaaaacgca actaaaagca aaaattagca aataagatca
      841 aattaaggca aaacccagat gttgtatgca tcaaataaag gcaaatatac gcaaactgga
      901 atttgtatgg tgaggcaaga tgtgtaatgt aatagtacac gcaaggtgaa ctactacatt
      961 accgacaaca attcgctatc gctcaatgtt ggatcttgtc ttatggagtt tgttatatga
     1021 aaaatttcga cttacaaata aataagataa cagcagtaaa aaatccaatt tactatggtg
     1081 atgatttctt acatttatgt atttatactg aacaggcaag ttatattcaa tttttttgcc
     1141 tagatttatt ttgctatgta aacaatatta atgagttgct tgcatgtatt cttcacaaag
     1201 aaacaagggc agattattca tttatagaat ggattgttaa tacagagttt aaagaaatat
     1261 ctatccaaga attgaaaata caaattaata atctaataaa tcaattagat attatttcaa
     1321 aagaacttcc aactaatcta agtgctaaat tattaaaaga atacttatgt actttaaatt
     1381 ttgaaaatat cacaaacagt tttgaaatct tagggaatca taaagttgtc ccgaataatc
     1441 ctgataatgg aggtccaaat tatcaatttt tttgggaaaa ttcggaaaaa atttattttt
     1501 tacacattca tttagaatca taatttaatc tatttaacat aatgggcgtt atccgaaatc
     1561 aactttttta agttaaataa aatcatatac ttaactattt tacatcaccc gtaaccaccg
     1621 caaaaatccg ctttcgaaac aaagcggatc ttttttgagc atgtttgata cttcttgcca
     1681 ataaaattgg ttaaaaaata atcaaaatcg caacttttta cgatttctta aggcttccat
     1741 ttctgaattt atccccattt tctgcggaca attcttggga taattttttg ggcctttctc
     1801 tgtacgttct ggcttacatt tattcaggaa attctctaat agccaggcga tcctcgattt
     1861 cctatcatgg attcatcaag aacaggatgt tcgaagacct aaaacaataa tcataagtca
     1921 cacaccagga tcgtgaagct agacaggagt cacgcttaag caccaaatac gaaaaacccc
     1981 gacaggatgt cgggggtttt ttatttatga ttaatgactt atatttcctc gatttgttta
     2041 atatcgataa atttattatc tttattttca tatagttggt aatggcaacg tgagcctacc
     2101 cccatattta aagtataatt tttgttttct tcaaatttca ctttaaaaac tttcagagga
     2161 catgatattg aacgtatatt atctacgtta ctataactca ttcttactaa gataaattta
     2221 tctgctggta ctttaaattc tccaaaatta gagaagtcct taggtgtgtc aggcattcct
     2281 aagcttttag gttgataagt aactttcatc aagcctgctc ggtttcgttt tgttattgct
     2341 gctccaccat caggatcttt caacatatct tttcgtacta atgagtttgg ataaatagat
     2401 acatctgcgc caatattata aataactctt acctgtgctg ttttccctgt tgagggttct
     2461 tgatatcttt tccccggatc ggtaatagca tcatctaatt tatagagagt agcgcaacca
     2521 gaaagcatta atagtgtgga taaaataact ataattttca tatatttaat acaactttta
     2581 agatatggtt ttgaatttat atactataga gtatgagtca aaattacttt tatctaatta
     2641 tatagattcc taataattga aactatcttt agctacgata tgaaatactt gcttttaact
     2701 tcgcataaga gattttatgt taaatcttaa acataaaaaa aggtaaccac tggttacctt
     2761 tttttatgtg tgataatatc ctcataactt agtataattt tttaaagata tggcaactgg
     2821 tattagtaaa aacgcttttg atgaagcgtc aaccttggct ttattgaggc acaaaaatcc
     2881 ctatccgaca gtacctaata caccaataga aattagttat aaaaataagt tagctgaatt
     2941 tgagattttg aagcccttga tttctgaatt tatagatgtt ggtggtaaca agctaaaagt
     3001 aaatatagag ggatctgatt tagtacttgg agataatatt tttgctttac atcagctgat
     3061 taaacaaaat acaaaagttg atcttatcta tttagatccg ccatacaaca caggaatgga
     3121 ctttgaatca agatctcagg aacatgctta taatgatagt tttgcgatag cttcttattt
     3181 ggaagatatg agaatccgtt taattcttat gcgtgagatt ctgtcagatc aaggtagcct
     3241 ttatctgcac attggtcatc agatgatgcc ttatttaaaa gtaattttag atgaagtatt
     3301 tggagttaaa aactgtcgta atattattac tagaagaaaa tgcagtagta aaaactctac
     3361 aaaaaatcag tatgcaaatt tgaatgacta tattcttttt tatacaaaga ccagtaacta
     3421 catttggaat cctcaggggg agaagcctag tgatgaatgg attcaaaagg aatatccaaa
     3481 agttgactca aaagggcaat ataaattagt tcctattcat gcacctggta aaagaaatgg
     3541 gtcaacaggc actgaatggc gtggaaagat gcctcctgaa ggcaagcatt ggcaatatac
     3601 gccagagaag ttagatgcac ttgatgaagc aggagaaata cattggtcta aaacaggaaa
     3661 tcctagacgg aaagtatatt taaccaatga taaaaaatta ccttataacg actattgggc
     3721 tgattttaga gatgcacatc atcaaagtgt aaaaattact ggatacccaa cagaaaaaaa
     3781 tcttcatatg ctaaaaatga tagttgcggc aagtagtaat acaaatagct tagttttaga
     3841 tcctttttgt gggtctggta caacacttca agctagtgct gagttaggtc gtcaatggat
     3901 cggtatggat caatctttta tcgcaatgaa atactctatc aatcgtttaa cgaaaggaac
     3961 gaaagaaatg ggagattatg ttttatcaaa acaaacctcc caattagatt taatagaaag
     4021 tgaaaatcta cataatttca ggctattggt agataaagat atctatatag aatatcaaga
     4081 tcaggttgat aatcttttat aaccaattaa gagtcacttc ttctaaggtc tttaaagtag
     4141 tgactcttac attatcatcc caactatcat ctaaacttaa tgtttcttcc catgtatctt
     4201 tatgagctaa tccgggtcca tctgatacaa atagaacttt aattacttcg ccagttgatg
     4261 agtaatgatc tttaagttga ctcacttttt gagagtttcc gcctgttctg tcatcagatt
     4321 gagctcctcc tctggtggag tcataacggc aaaatccaac agctaaaact ttttgagtat
     4381 tagtatcttt tattacaaaa tcacaaggga atttaccacc tttaaaagat ggataaactg
     4441 tatttagttc aatatctttc cctgcgcctc tagggcctaa tatttcagta tgagggtaaa
     4501 gtctctctag ttcatcgaaa actaaatcag ttaactcata tccagatgag cctcgattat
     4561 cgtattcagc taataaacat gccaaactta aaagatgagt attatcaaat acattattaa
     4621 aaaaatgctt tagattggct atatcggcaa aatagtcttg gaacgtattt actaaatctt
     4681 gtgtagaaac tttcgttatt tttttagaaa gctctgtatc taaactacta cacacacatc
     4741 gacgaaataa ttttagatat gacatacgag tatctttatc tattgaagat actcgtattt
     4801 ttcctaataa atctgctgag ctagtggata gattaactag accgatgaaa cctcgaatag
     4861 gcttttcata tttttttaag gcaatctcag aatctaatga aaaattagct ccattagata
     4921 gaacagtaat aatttcataa ggatcgtgtt ggtatttatt aataattccc acagtagggc
     4981 cctctataga ttgaggccca aagtgtatgc ctaataaatc aattagttaa tccttcacaa
     5041 agagtttttt tcaatcgatc aaagtcatgt aacatgtctt cctttctatc atataaccaa
     5101 tcagcactca tggaatttgt tagtgcaaag cgaattccga acttaccact ttgtataacg
     5161 ccaaattgtt gatgagcgta agtgtgaata aatgcactca cgtgcgttac ttcagtagga
     5221 tttataagag ggttgatgaa gtcatgtgaa agagaggatt ggtgatcata tctgtcacta
     5281 aaatttaccc cgattgtttt cgaatctaaa aaaaggatag ccattttttt tcctaaataa
     5341 gtattatgaa ttacgttttt tatcaatatt taatctatat gagtcttctt gcttcataca
     5401 ctctagatca tgctttagga aatctctctc aaaatccatt aagtttttga taaaattaat
     5461 ttttctatct ataagtaaaa tttttttatc tacatcttct gtcgatacta ttaggtcatc
     5521 taactgctgt aaaaaaagtt cttcatgacg aagtaatgaa aatttcatag ggatactcag
     5581 tataaaaaat agatgttaat ttttctaaaa ttaacataat acgccttatg cgaaatactt
     5641 gaacttttcc ttttcgcatc aatatcttaa agtaagccgt tcctgggttc agcgcactgg
     5701 aatggctttt tcgtgatctt tcacgttcca cgcctattcc gtgatcattt ctctatattt
     5761 ttattcatct agattaaaca atcttgatta atcatagggt ctaaaaaaac gactccaaaa
     5821 ccctcttttc tccggctctg gttcaacatg ctctggtatt ggaatacgct tattctcatt
     5881 cgtatctagt tctttctgcg acatggtgat gacttcttct ttttcttttg gtatctgctc
     5941 agcctgagta ggttctgggt cgacagacgt agggatatga ggttttgggg cctctagtaa
     6001 acgttgcata gcttcaattt gttcttgata gaaagattca cgttctaatg attgattctc
     6061 tctaagtaaa gcttgattca attgtttttc aagtatgtca acttgtcgtt tcaataagtc
     6121 aacttcagtc aagttttgac tgttagttga ttgactttga ttgacattgg atgttttatt
     6181 ttgaggctct ccgaatactc ttaaagcttc tgaaaagtca attaacccat cagaattttt
     6241 tgatagattt cctttattta tatgagcata tatagcttgt cttgaatatc catacaactt
     6301 agctaattct gaaactgata attttttcat attgtaaatt gagggggcgt tgagttaata
     6361 ctttactgtt aatttgacaa tgtcaattta caaacctatt taaatcctaa ttcttttaga
     6421 tatggagaat attctttaat ttttttagga tctgttaagt ctccagatat tctaattata
     6481 aagtcatcat agctttcacc agttttacta tattttgatg cgaatgtggg gtcataagct
     6541 aacttaccag agaacaaaag aatctgttta tctgtcaatt gaccaatcaa ttttagctgt
     6601 tgattacttg ttttcgaagt atttttacca gatgtctttt gcttaaatga aaaactataa
     6661 cctgaaattg atcgcccagt tttatgctgt tcagacttaa ctttgatgtc tgtaaaagta
     6721 ttgatttggg atatagctat atctagaact cttcttttga aattatctga gcgcgtgtat
     6781 tcatcgtctg caataccaag ttgctgccta aattctgaaa cttcaaatac tggagtctga
     6841 ccagtggtgc gccaagcaac taatagctcg taaagtctag tggcatatct gcttgttaat
     6901 tgacttactt gctttaattc atagctagta aaccgttgct caagccttgt aattaatggg
     6961 acgacagctg gtgaaaagat aagacttact gatgcttcat tatcaatata gctaatttca
     7021 cttacccaac ggctatgagt agtttttaca ttccctttat cggttaaaga ttgataagta
     7081 aatctcctat caaataagac cttacttgcc tctttcaaaa cagcataagc cgtatgtttc
     7141 tctacaccga aattctcaat atagcttgat gcatgaatta ccaatgcatc attagcattg
     7201 attcctttac cagtttgtct ggcttcaatg attgctaaca gaactagtct ttgctcaact
     7261 aaatcaaggt tataacttgc attaattaaa gcattatctt tgacaactaa gtctttcatt
     7321 atttttatta ctacatcata aaatgatgta gtaatactac ataaaatatt aatgtagtca
     7381 ataggtcgat aaacgtagtg atataggtcg ataaacgtag tgatataggt cgataaacgt
     7441 agtgatatag gtcgataaac gtagtgatat aagctcttaa actattatgc ataaaggctt
     7501 acatctaatc taaaagtatt taaaaaattt aaaattatta aaaacactgg gaatgaaaaa
     7561 ttgccctttg ttttcgcttc gctcaaactc tattgaatct cgctttcgct cgattcgagg
     7621 ggcaattttt tggcttatgt ttccgtgaat ttgagaaaaa gcaaaagcaa ctcggaattc
     7681 gcttcgctca taaaactttt ttactcgcta cgctcgatct agactcaatt ttctgcacgt
     7741 aatttacgtt tatttagctt tcacagcctc aaaagctgtt tttcagagag tggaggtgcg
     7801 aattttggta ctttgctcgt agacactcgc tctatttatc ccgattagaa taaaaatagc
     7861 cgctatttag cactcatttg cattttttga cctgattact catttcaata gttttcctat
     7921 gtcttaagta aaaacttggc taaaatcgca tgtgagagcg ttttaactta ggttttattt
     7981 ttgatctgta gattagagtt cccgcttata gatttttccc ttagaaacag aatagttcga
     8041 atctctttta gccaagcact atctttactg tcagttaagt caattaaatg gattgagagc
     8101 c
//
