LOCUS       NZ_CP061549             3744 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS80 plasmid pAS80-6, complete
            sequence.
ACCESSION   NZ_CP061549
VERSION     NZ_CP061549.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092247
            Assembly: GCF_014717695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 21:54:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,827
            CDSs (total)                      :: 3,733
            Genes (coding)                    :: 3,385
            CDSs (with protein)               :: 3,385
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 348
            CDSs (without protein)            :: 348
            Pseudo Genes (ambiguous residues) :: 0 of 348
            Pseudo Genes (frameshifted)       :: 280 of 348
            Pseudo Genes (incomplete)         :: 59 of 348
            Pseudo Genes (internal stop)      :: 27 of 348
            Pseudo Genes (multiple problems)  :: 17 of 348
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061549.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS80"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS80-6"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            join(2450..3744,1..139)
                     /gene="mobQ"
                     /locus_tag="IC800_RS18915"
                     /old_locus_tag="IC800_18915"
     CDS             join(2450..3744,1..139)
                     /gene="mobQ"
                     /locus_tag="IC800_RS18915"
                     /old_locus_tag="IC800_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_191110361.1"
                     /translation="MAIFHFSVKAISRSAGRSAVACSAYRSGEKLEDHYQGKIQDYTQK
                     TGVESKKIYAPENTKKELLDRQELWNAVEQVERRKDATLAREFEIAFPHEFNAEQRQDL
                     LDELCQEIVEKHGVIVDACIHAPHTKSGSDERNFHAHIMFTSRQIDPQTGDFAKKKNRD
                     FNKEQSSETVSHWREHFAELTNRHLEKHGFADRVDHRSYKDQGIDLEATQHEGPQVTQL
                     RRQGIDTEISLSNDAIRERNQFSQHIKGLDQEIQASERLLNNLIQTREKLDQEQRQKEK
                     DQEQRSPEELTKIAYSVISQYNDAVATEAKNIALTTQKTEIEQINAVLEQIELSKTNLE
                     HQKEQLGKRPMFFGGKWDAANAEIQTQWQELDSQERDLKTKTPRLEPERYTDRAKEIVN
                     KANPNLKAKHDRARLHLEREQQRKLAEQEKRKQEAKAQQEREKQAFFARKALREQGKHD
                     EANKLLAESKTQSHKPRSR"
                     /name="mobQ"
     gene            795..1736
                     /gene="repM"
                     /locus_tag="IC800_RS18920"
                     /old_locus_tag="IC800_18920"
     CDS             795..1736
                     /gene="repM"
                     /locus_tag="IC800_RS18920"
                     /old_locus_tag="IC800_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005167830.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_191110362.1"
                     /translation="MKNSLVVKDNALINASYNLELTEQRLIMLAIINARESGQGITADS
                     KLEIHASDYAKLFNVSIDASYKALREAVNNLFNRQFSYTAEYKRTGKTGIVRSRWVSRI
                     FYVDDLALLEITFAPDVVPLVTRLEEHFTSYQAKQVAHLTSKYATRLYELLIAWREVGK
                     VPQIEIGDFRNRLGLLESEYTAMSDFKKRVLEPSIKQINEHTDIAVTYEQHKKGRLISG
                     FSFRLKQKQQPKIEKPVDPKRDPNTPDFFIPMTDAQRHLFAHKLSEMHEMSEYSVGTES
                     YEDFAKRIADMLLEPEKFRTFYPLLVQAGFNY"
                     /name="repM"
     gene            1746..1943
                     /locus_tag="IC800_RS18925"
                     /old_locus_tag="IC800_18925"
     CDS             1746..1943
                     /locus_tag="IC800_RS18925"
                     /old_locus_tag="IC800_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110363.1"
                     /translation="MTDRQQLLEAFRSAKGIVDHEFTQEIIDSCQLEDQARQQRILNGE
                     TTFEQEDAALMEEWYKLNSK"
                     /name="hypothetical protein"
     gene            complement(1944..2225)
                     /locus_tag="IC800_RS18930"
                     /old_locus_tag="IC800_18930"
     CDS             complement(1944..2225)
                     /locus_tag="IC800_RS18930"
                     /old_locus_tag="IC800_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_151778663.1"
                     /translation="MKVESLEQQIAKQEERLKQLKAQKQAVLAREKKKATDQQRKEDTR
                     RKILLGSLALKKMQDDSEKAKILADLNEYLTEDRDRLLFGLQPVSNAG"
                     /name="mobilization protein"
     Typing          796..1706
                     /name="rep_cluster_1219"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.104"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ggaagccaag gcacaacaag aacgagaaaa acaggcgttt tttgcacgta aagccttacg
       61 tgagcaagga aaacatgacg aagccaataa actactggct gaatcaaaaa cacaaagcca
      121 taagccacgt tcacgctaaa atttaaaatt tcttttttgg cgtttattaa aattatctaa
      181 acaatacatg gtcttataga gcgagtgtct acgagcgaac tagcaatatt cgacgctcga
      241 ccctctgaaa aaccgctttt aaataatttg aaacgctaca agccttatgt agcaatgctt
      301 tgatggctta tacacctacg tttaccttgc aatattccta ggtttagttt gcagaaaatt
      361 cgcccctaga ccgagcgaag cgagcataaa gctttatgag cgaagcgaat ccatagtttc
      421 ttttgctttt agatttcaca aacaggacta accaaaaaac tgcccctcga atcgagcttg
      481 cgagattcaa tagagtttga gcgaagcgaa aacctagggc agttttgacc actcccctac
      541 cccaaatggg cttttaataa gcttttaagc ttttaaatgc ttttagacag gctgaaagca
      601 ttgatatacg gtcattgcag agcttataaa cggacagtag ctcttatata aacggacagt
      661 agctcttata taaacggaca gtagctctta tataaacgga cagtagctct taattaaggg
      721 acataaaata tattgacctt aaaaggatat tggaatacat tgtctcttaa tttgataata
      781 atatgggctg ttttatgaag aacagtttag ttgtgaaaga taatgcttta atcaatgcca
      841 gttataactt ggaattaaca gaacagcgtt tgattatgct tgccatcatt aacgctagag
      901 aatcagggca gggcatcaca gcagacagta aactagaaat acatgctagt gattatgcaa
      961 aactgtttaa tgtgtcaatt gatgcgtcat ataaagccct cagagaagcc gtaaacaact
     1021 tgtttaatcg acagtttagc tacacagcag agtataaaag aacaggaaaa acaggcattg
     1081 tacggtcacg ttgggttagc cgaatttttt atgttgatga cttggcatta ctagaaataa
     1141 cctttgcacc tgatgttgtg ccactggtga cacggttgga agaacacttc acaagctatc
     1201 aagcaaaaca agtcgcacac ttaacgagta aatacgcaac aagactgtat gagcttctca
     1261 ttgcgtggcg tgaagtgggc aaagtcccac aaatagaaat cggtgatttt agaaacagac
     1321 tagggctttt agaaagtgaa tacaccgcaa tgagtgactt taaaaagcgt gtactagagc
     1381 cgtctatcaa gcagatcaat gaacatacag acattgcagt aacgtatgaa cagcataaaa
     1441 aagggcggtt gatttcaggc ttttcattca ggcttaaaca aaaacaacag ccaaaaattg
     1501 aaaagccagt tgacccgaaa cgagatccaa atacccctga tttctttatc cctatgaccg
     1561 atgcacaacg gcatttattc gctcacaagc tgtctgaaat gcacgaaatg agtgagtatt
     1621 cagtaggtac tgaatcatac gaagactttg caaaacgtat tgctgatatg cttttagagc
     1681 ctgaaaaatt caggacgttt taccctctat tggtacaagc aggatttaac tattaggata
     1741 ttgaaatgac agatagacag caattactgg aagcctttag aagtgctaaa ggcatcgtag
     1801 atcatgagtt tactcaggaa ataattgatt cttgccaatt agaggatcaa gcaaggcagc
     1861 agcgcatcct gaacggtgaa accaccttcg aacaggaaga tgcagcactc atggaagaat
     1921 ggtacaaatt aaactcgaaa taactaacca gcgttagaaa caggttgtaa accgaataac
     1981 aatctatctc tatcttctgt taaatactcg ttcaagtcag caagaatttt tgccttttct
     2041 gaatcatctt gcattttttt taacgccaaa gaaccaagca aaattttacg tctagtatct
     2101 tcttttcgct gttgatccgt tgctttcttt ttttcacgag ccaaaacagc ttgcttctga
     2161 gctttaagct gtttcaaacg ttcttcttgt ttagcaattt gctgttccaa agattcgact
     2221 ttcattacaa atccaatgcc taaaaaacca cagttagcat aagcgtacat gaattgaaat
     2281 tcaaggtact atctgctatc gtggtttttc ccaaaaggcg cactttacca ttgaaaaaaa
     2341 tcttcgattt ttattcaatg gtagtacggc aggggattcc ccctacaacc ccaaaccagc
     2401 ttatgcaagc tgggaaattt ggaaacccaa atttaagagc aaaagcaaaa tggcaatctt
     2461 tcatttttca gtcaaagcga taagccgaag tgcagggcgt tcagccgttg catgttctgc
     2521 ctatcgttcg ggtgaaaaat tagaagatca ttatcaaggt aaaattcagg actacaccca
     2581 aaaaacaggt gtggaatcta aaaaaatcta tgcacctgaa aacaccaaaa aagaattatt
     2641 agaccgtcaa gaattatgga atgcagttga acaggttgaa cgtagaaaag atgcaacctt
     2701 agcaagagag tttgaaattg cctttcccca tgagtttaat gcagaacaaa gacaagactt
     2761 gctcgatgag ctatgtcagg aaattgtaga aaagcatggt gtgatcgtgg acgcatgtat
     2821 ccatgctcca cataccaaaa gcggaagtga tgaaagaaac ttccatgcac acatcatgtt
     2881 caccagtcgg cagattgacc cacagacagg cgactttgcc aaaaagaaaa atcgagactt
     2941 caacaaagag caatccagtg aaaccgtgag ccactggaga gaacactttg cagaattgac
     3001 caaccgccat ttagaaaaac atggctttgc agatcgtgtc gatcaccgta gttacaaaga
     3061 tcaaggcata gaccttgaag ccactcaaca tgaggggcca caagttaccc aacttcgcag
     3121 acaaggcata gacaccgaaa tcagtctatc caatgatgcc atccgtgaac gcaaccaatt
     3181 tagccaacac atcaaaggtt tagatcaaga gatacaagca tctgaacgac tgttgaataa
     3241 cttgattcag accagggaaa aattagacca ggaacaacgc caaaaagaaa aagaccagga
     3301 acagcgttcc cctgaagaat taaccaaaat cgcatatagc gttatttcgc aatataacga
     3361 cgctgtagcc acggaagcaa aaaacatagc actaaccacc caaaaaacag aaatcgagca
     3421 aataaacgct gttttagagc aaatagagct atcaaaaacc aatcttgaac accaaaaaga
     3481 acaattaggc aaaagaccta tgttttttgg tggaaaatgg gatgctgcaa atgcagaaat
     3541 tcaaacccaa tggcaagaat tggacagtca ggaacgagat ttaaagacca aaaccccacg
     3601 tttagaacct gaacgctata cagatcgggc aaaagagatc gtaaacaaag ctaatccgaa
     3661 cctgaaagcc aaacatgatc gagcaagact tcaccttgaa cgtgaacaac aacggaagtt
     3721 agccgaacag gaaaagcgga aaca
//
