LOCUS       NZ_CP061548             5091 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS80 plasmid pAS80-5, complete
            sequence.
ACCESSION   NZ_CP061548
VERSION     NZ_CP061548.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092247
            Assembly: GCF_014717695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 21:54:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,827
            CDSs (total)                      :: 3,733
            Genes (coding)                    :: 3,385
            CDSs (with protein)               :: 3,385
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 348
            CDSs (without protein)            :: 348
            Pseudo Genes (ambiguous residues) :: 0 of 348
            Pseudo Genes (frameshifted)       :: 280 of 348
            Pseudo Genes (incomplete)         :: 59 of 348
            Pseudo Genes (internal stop)      :: 27 of 348
            Pseudo Genes (multiple problems)  :: 17 of 348
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061548.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS80"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS80-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            complement(join(4946..5091,1..140))
                     /locus_tag="IC800_RS18885"
                     /old_locus_tag="IC800_18885"
                     /pseudo=""
     CDS             complement(join(4946..5091,1..140))
                     /locus_tag="IC800_RS18885"
                     /old_locus_tag="IC800_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            354..1305
                     /gene="mobQ"
                     /locus_tag="IC800_RS18890"
                     /old_locus_tag="IC800_18890"
                     /pseudo=""
     CDS             354..1305
                     /gene="mobQ"
                     /locus_tag="IC800_RS18890"
                     /old_locus_tag="IC800_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     gene            1302..1771
                     /locus_tag="IC800_RS18895"
                     /old_locus_tag="IC800_18895"
                     /pseudo=""
     CDS             1302..1771
                     /locus_tag="IC800_RS18895"
                     /old_locus_tag="IC800_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1850..2248
                     /locus_tag="IC800_RS18900"
                     /old_locus_tag="IC800_18900"
     CDS             1850..2248
                     /locus_tag="IC800_RS18900"
                     /old_locus_tag="IC800_18900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110360.1"
                     /translation="MAKAIFQSVELYFHFYVLSICHKEAYFHSTNSGLNNENVFDFPGF
                     YNNIFILTLVVKSNNDRKKFEVFNEIETTNKLIQKNHKKIMVFIFSNGFVAQRFHNYMI
                     LKVLIIFDPKLNLFKSDAYIFMQQSLFK"
                     /name="hypothetical protein"
     gene            2452..2655
                     /locus_tag="IC800_RS19300"
     CDS             2452..2655
                     /locus_tag="IC800_RS19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004896916.1"
                     /translation="MFNLNIFNKISSEVLTIKNDLELNSENQLITKYKTSTSEDYKKAI
                     ILIFKERGYTRLEIGQLLEGQL"
                     /name="hypothetical protein"
     gene            3368..4347
                     /gene="repM"
                     /locus_tag="IC800_RS18905"
                     /old_locus_tag="IC800_18905"
                     /pseudo=""
     CDS             3368..4347
                     /gene="repM"
                     /locus_tag="IC800_RS18905"
                     /old_locus_tag="IC800_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870947.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /name="repM"
     gene            4344..4638
                     /locus_tag="IC800_RS19370"
                     /pseudo=""
     CDS             4344..4638
                     /locus_tag="IC800_RS19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4654..4770)
                     /locus_tag="IC800_RS19305"
     CDS             complement(4654..4770)
                     /locus_tag="IC800_RS19305"
                     /inference="COORDINATES: protein motif:HMM:NF012442.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_307880253.1"
                     /translation="MGHNPKTGEKLKIEAKEIVTFKQGKALGEQFIAKPKQK"
                     /name="HU family DNA-binding protein"
     gene            complement(4788..4943)
                     /locus_tag="IC800_RS19310"
     CDS             complement(4788..4943)
                     /locus_tag="IC800_RS19310"
                     /inference="COORDINATES: protein motif:HMM:NF012442.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_307880254.1"
                     /translation="MNKADLIAKLAPKLNINQDEARVVLNSVLEVITEALVEDKNVRLV
                     DFGTLK"
                     /name="HU family DNA-binding protein"
     Typing          3369..4347
                     /name="rep_cluster_1071"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.294"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          485..1302
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.255"
                     /coverage="100.0"
                     /evalue="4.44e-147"
ORIGIN
        1 tcgagtatca tcttttcttt cctgctcttt ctgctttgat tttctctagc ttcgatagct
       61 tgtttgcgag cctttaactg tttcagcttc tctaattggg cttcaatttt cttttcaaga
      121 ttttgagcgg cttttgtcat gattcagatt tcgggatata gaaaaccact caagcataag
      181 gccctatgaa ttgaaattca agcgggctat atgctatcgt gtttttcatg aaatgcgcac
      241 ttacgacttg gatttcatcc aagtcaaagt ttgcgtaagg ggatacccct taaccccaaa
      301 aattgctacg caatttttta aaagcaaaag caaaaccatc acaaggctaa ggcatggcaa
      361 tttatcactg tacgactaag accgttaacc gaagttcggg acgaactgcg gttgcctcta
      421 tggcttatcg tgcagggaaa agttgacaga tgaacggaca ggattaacgc acgacttcac
      481 gaaaaagaag gtgtagtgta tacagagatt ctttctaact tggatactga attagaccga
      541 agcaaagtgt ggaatctggc ggaaaaatca gaaaatcgga aagatgcccg aaccgcacgt
      601 gaatgggtga ttgctctacc agacgagctg gatgaagaac agcgaaaaga actagcaaga
      661 gagtttgccc aatctttagt agaccgttat ggggtggttg cagatttagc gattcatgca
      721 cctagcaaag gtggagatga taaaaaccat catgcacata tcttactgac aactcgcaaa
      781 gcagaattag atactgaaaa taaactggtc ctcactcaaa aatcggaaat tgagttaagt
      841 aacaccaaaa gaaaaagctt aggcatggga acaagccagg aagaaattaa acagattcga
      901 gccacctggg caaacttagc caatcatgct ctggaatatg cagggtatcg ggaaagaatc
      961 gaccaccgca gttatgccga tcaaggtaat cagctacaag ctaccataca tgaaggcagt
     1021 aaagtcaccc agatgcgcag aaagggcata gatactgaaa ttagccgttt caacgacacg
     1081 atcaaacagc agaacagtca acagctccaa tacaaacaac agcacaagga acagacctta
     1141 gaacagggtt tcaaccgtgt tgaaaaaggc tttgagcaat ggaagaaaga tcaggaagct
     1201 aaacgcctag aactagaaca taaaaagcag ttaaagctac agcaagaaca agcgatgaag
     1261 ctgaaacaac gcaaaagcat gaatagaaat gggccttccc tatgagcaat caaaacgatt
     1321 tagacgacca gttatatatt ttattagcct ccatgaaaga atacaggaag ccatagcaga
     1381 tgataacaag cgattagaga cgttttacaa taaggtagct agtggggtac tagaacagag
     1441 caaaaaaacc ctaaataacg caaatcagga agctacacac gcccttcaag gtcgtatcca
     1501 ggagttagat aaagccaccg ataaattgaa ctatagattt attgccttgt tgtgtgcaat
     1561 tttccttagt ctggtattag ttttcctgtc ttttatattt ttatttattc caagttttga
     1621 tgaaatccag cagcgtcgag cagaagcagc ttggttagaa cagagttaca acctagatat
     1681 taaaaattgt aatggtaaag cttgtgtacg aatcatgaaa aatgcctgtc acggaacgaa
     1741 taaagattac tgtgtgattg atcctaaata attactggta agtactttgc tcggttcttc
     1801 tcttaatctt atcaataccg gatgtcgcaa agtggctcga ctttgaaaca tggcaaaagc
     1861 catatttcaa agtgtcgagc tttactttca tttttatgtc ctatctattt gtcataagga
     1921 agcatacttt catagcacta atagtggact taataatgag aatgtatttg attttcctgg
     1981 cttttataat aatattttta ttttaactct agttgttaaa tccaataatg atcggaaaaa
     2041 atttgaagta ttcaatgaaa tagagacaac aaacaagttg atacaaaaaa accataaaaa
     2101 aattatggtt tttatatttt caaatggctt tgttgcacaa agatttcata actatatgat
     2161 tttaaaagtt ttgattattt ttgatccaaa attaaatcta tttaaatcag atgcttacat
     2221 atttatgcaa caaagccttt tcaaatagtg ttttagcaaa aataatcaaa tttcgtataa
     2281 tcgccattat gttaaatgga tggcatttgg gagatattat ttattctttc aataggcaga
     2341 aacttgcaga attttcttaa tttgtaatcc ttgcggctaa gggtgtctag cgtagctaag
     2401 acaggtattc gttatagtcg ggccgctata atacggatga cgagtgcctg tatgtttaac
     2461 ctaaatatct ttaacaagat ctctagcgaa gtattgacca tcaaaaatga tcttgagctg
     2521 aattctgaga atcaactgat aactaagtat aagacttcta cttctgaaga ttataaaaaa
     2581 gcaattattt tgatctttaa agaacgtggc tataccaggt tagaaatagg tcagttactt
     2641 gaaggccaac tctaaagact tcctaaaatt aacataatac accttatacg aaatacgtta
     2701 taattccact tagaaatcaa tgctgtagtt tctagtttat agcccatctt ccccaaggtg
     2761 ggctttttta tgattttcac gttccacgcc tattatttaa gcaatgtttc acaactttga
     2821 ttagcttcat taaataatta aaacctagat aataagaacc gagcgagtgt ctacgagcga
     2881 ttgagcatca tcaaaatttc acgctcgccc tttctctgat tacaagcttc ctttagattt
     2941 taaacggtag atacagagca tcccgaatgg gtgcgaactt tggagctttt gcttttcaaa
     3001 agtatgagcg aagcgaatac attaccaatg cttttgcttt tagatttcac aaacaggatt
     3061 aaccaaaaac tgcccctcga agcgaggaac gagcttcaat agaatacgag ctttagcgag
     3121 tacctagagc agttttagca ctccctcttt ttaataattt tataatttta taattttaga
     3181 ccacttattc atattaataa tcaatggctt atattgctat atctatacgt ttatgcagtc
     3241 ttatctatac gtttatgcag tcttatctat acgtttatgc agtcttatct atacgtttat
     3301 gcagtattgc tatacgtatt taatctatgt aatataatta caactaaaaa gaatgtatgt
     3361 ataagaaatg gcaaatgatt tagttgttaa aaataatgcg ctgattgatg caagttatac
     3421 cttgagctta gtagaacaac gcttaattgg attggctttg gtcaaagcta ataatcagca
     3481 ccaagaaatt accagtgata cagttctgac catacatgca gggaatatgc tgaacaattt
     3541 aaggttgatg gttcagtagc ttatcgagca ttaaaagaag catcagaacg gttgttttta
     3601 cgctatttct catacacctt atatggcttg gaatttggta aggaatacac ttttaaacgt
     3661 cccaaaaagc taagagattg cgatatacca accgttatga aatcacgatg ggtacaaaga
     3721 tcggttatac agaatcagag ggtttattac acttccaact taccagtgat gttgtcctat
     3781 tggttgctaa ttcaaaagaa tatttcacaa gctattactt atcacaaact accgacttta
     3841 ccagtaccta cgctactcga ttgtttgaac tcctaatgaa atggaaaaat gtcggacata
     3901 ttccatttat tgaaatagaa caattacgag ggcaattagg tgtagaacct aagcaatata
     3961 aaattatttc taactttaag ttgcgagtcc tagatgtggc tgtggagcaa gtgaatcaac
     4021 attcagacta tacgattaaa tacgagcaac ataagcaggg gcggacaatt ataggttttt
     4081 cattcaaatt taagcctaaa gtagataaaa tctcaaaaaa aataatatct aagcaaaatc
     4141 gtagtaaccc tgatttttat aaaattgagc gatccacaac gccatttatt tgcaaataaa
     4201 atgtctgaaa tgcccgaaat gagcaaatac tcacaaggta cggaaagctt tcagcaattt
     4261 gctattcgta tcgctgacat gcttttagag cctgaaaagt tcagagagct ttacccatgt
     4321 ttagagaaag cagggtttca agcatgatga aattaactaa cctattagaa gaatttcaag
     4381 gcattcaagc tgaatacctc gacatcgtta attatgaaat tgcaagggaa aatatatgcg
     4441 gttatatctt cctattatcc cgaatatcaa aaaagccgaa cctatcgaaa aatacagata
     4501 gaaagcaaaa tagaggactt aatttactat cgggataatt tgcagatcga agatatagaa
     4561 aatatccaaa agatcttaaa tgagctaatt ccagagtata aagccgaaca agaaaaacaa
     4621 agagctaaga aaaattaggc tttcttctta gctttacttt tgctttggct tagcaataaa
     4681 ttgctctccc aaagccttac cttgtttaaa agtaacaatt tccttagctt caattttaag
     4741 tttttcgcca gttttaggat tatgtcccat tctttcggca cgctgtttta cttcaaagtc
     4801 ccaaaatcaa ccaaccgaac atttttatct tcaactaagg cttctgtgat tacctcaaga
     4861 acagaattta gaaccactct tgcttcgtct tgattaatat ttaatttagg tgctaatttg
     4921 gcaattaaat cggctttatt cataattaat tttcctcaat atttggtaag tcaaaaagct
     4981 gtctatctct atcttctgtt aaatactcat tgagttcagc gagtattttt ctttgttggc
     5041 ttcattactt tgcatctttt taattaaata agaaccgagc aaaattttac g
//
