LOCUS       NZ_CP061547             5838 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS80 plasmid pAS80-4, complete
            sequence.
ACCESSION   NZ_CP061547
VERSION     NZ_CP061547.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092247
            Assembly: GCF_014717695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 21:54:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,827
            CDSs (total)                      :: 3,733
            Genes (coding)                    :: 3,385
            CDSs (with protein)               :: 3,385
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 348
            CDSs (without protein)            :: 348
            Pseudo Genes (ambiguous residues) :: 0 of 348
            Pseudo Genes (frameshifted)       :: 280 of 348
            Pseudo Genes (incomplete)         :: 59 of 348
            Pseudo Genes (internal stop)      :: 27 of 348
            Pseudo Genes (multiple problems)  :: 17 of 348
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061547.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS80"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS80-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            complement(join(5745..5838,1..320))
                     /locus_tag="IC800_RS19270"
     CDS             complement(join(5745..5838,1..320))
                     /locus_tag="IC800_RS19270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="WP_307880248.1"
                     /translation="MAVVRWWSEKIGKADMVKDNSNYGIENRVYVTNTDKSKTLKDIDL
                     SGVSPYISQSLRLQDAFGLRREEAMKFQPEYALDGQSIDTAKYIRLKESWAKGGRARTI
                     PVTSEKQRQELKSALALAKDGSLIPPEKRIRHI"
                     /name="integrase domain-containing protein"
     gene            complement(1406..1726)
                     /locus_tag="IC800_RS19275"
     CDS             complement(1406..1726)
                     /locus_tag="IC800_RS19275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_307880249.1"
                     /translation="MKHGLDIRDGSYAQEYVAKWGLDYEMTKGHVKRGRKESLSPFDLL
                     QLSIEDQEIFEKLPSKLWQEFAISMKGARQLVWSRGLKNWLGLVRSQMKKLQMKQMKKV
                     SL"
                     /name="hypothetical protein"
     gene            complement(1822..2178)
                     /locus_tag="IC800_RS19280"
     CDS             complement(1822..2178)
                     /locus_tag="IC800_RS19280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_307880250.1"
                     /translation="MKHGKTNWGNLQRCGSVWSCPVCAKQITEERRVEMGQLIKYWEQQ
                     ENSDIKLMTLTFSHSKDEPLKVLLRKLKNAITMFLVVGSSKIYPSFARFNTKSVVLKSL
                     MVQTDGIRIFIFFY"
                     /name="hypothetical protein"
     gene            2832..3113
                     /locus_tag="IC800_RS18870"
                     /old_locus_tag="IC800_18870"
     CDS             2832..3113
                     /locus_tag="IC800_RS18870"
                     /old_locus_tag="IC800_18870"
                     /inference="COORDINATES: protein motif:HMM:NF038291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_307880251.1"
                     /translation="MARITVTQASKDFNISRNTLYKKIKQGHLTKDSNGLLDVNDLIRV
                     IGAHTESTSKNVTLNTSDIQSVSSDTQLQQAKSEVEQLKQLLAQKKFS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3521..3901)
                     /locus_tag="IC800_RS19285"
     CDS             complement(3521..3901)
                     /locus_tag="IC800_RS19285"
                     /inference="COORDINATES: protein motif:HMM:NF024108.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraG/TraD/VirD4 family protein"
                     /protein_id="WP_307880246.1"
                     /translation="MPTFLYFQSLEGLNRLYGNDAERLIMGSADLKVVFRIGDDETCQT
                     FSRMVGETEALIGGFAEVKGFTSTPNIHNPAILLNNDIVSNSGSKTLSKNTGILKVFEP
                     ENSNNWSNTQQFACIREDQPYH"
                     /name="TraG/TraD/VirD4 family protein"
     gene            complement(3871..4206)
                     /locus_tag="IC800_RS19290"
     CDS             complement(3871..4206)
                     /locus_tag="IC800_RS19290"
                     /inference="COORDINATES: protein motif:HMM:NF014581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_308436489.1"
                     /translation="MFTSLSKAFAFLDDDRIRKQVQRSDFSLKTTCSAKTNTFLQFEQE
                     FRSSTATIFGAMVAQTLRILQTNCRNRDAVFVALDEIINCAPIPNFTSTLNMIRSAKCL
                     PSSISKV"
                     /name="hypothetical protein"
     gene            complement(4273..4761)
                     /locus_tag="IC800_RS19295"
     CDS             complement(4273..4761)
                     /locus_tag="IC800_RS19295"
                     /inference="COORDINATES: protein motif:HMM:NF014581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_307880247.1"
                     /translation="MEDDLWSENRFKARVSNEDRGMVVGAPGSGKTAFLIAQVVDWMQS
                     GRSVVLTDVKPEIYGRLLEAGVFERYGYTPTVFNPTDIHSHAYNLFSESSSSAELNEIL
                     SVIIPYVDDSSEVFAENARRLLKAVLLELGEEASLPNAQNSLTPKIILLTFKNSEKIK"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            complement(5262..5501)
                     /locus_tag="IC800_RS18880"
                     /old_locus_tag="IC800_18880"
     CDS             complement(5262..5501)
                     /locus_tag="IC800_RS18880"
                     /old_locus_tag="IC800_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017386456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017386456.1"
                     /translation="MGIASEIRGNLPLCGRCINCKLLVWEEKAELKTSSNLIKFKSLDS
                     AYYYMVRCSWLKSPVNEPQFLEICEGKQEYGAKG"
                     /name="hypothetical protein"
ORIGIN
        1 gtaactggaa tcgttctagc cctaccacct ttcgcccacg attctttcaa ccgtatatac
       61 ttggctgtat caatcgactg cccatcaagt gcatattcag gctgaaattt catagcttct
      121 tcacgtctaa ggccaaaggc atcttgtaat cgcaaagact gactgatgta aggtgaaaca
      181 ccacttaaat caatatcttt taaagtcttt gatttatcag tattagttac atagacacgg
      241 ttctctattc cataattgct gttatctttc accatatctg ccttaccaat cttttcagac
      301 caccagcgga caacagccat tcggttttta tcgttccaat actttttcca tcctctttcc
      361 actttttgac caaggcattg atatgtctac cttttagatc actaggttgc atatgcttaa
      421 ctttatagcc gagacttttt aagtcctctg tcatctgtga caacatcgct ctacgattag
      481 cttgcgttgt atgtgatcca tccttatttc gatcacaaat ttgcatcagc ttatactcta
      541 acctattcat ttttataacc ttaaaaaaga acccctttta tgcgaggaca aatgtccttt
      601 tggacattac cacagcataa aagtaaatag gcaaatacag caaataaatg caatttatta
      661 attaaatctt gagccagttt tggtgggata gtcgtaatcg tttctttttt cgttaatgga
      721 tcaacgtgat ataaaaaaag cgctgtgaac cgatttgata accatcgaaa tgtacttggt
      781 cagcagatga aacttgtatt tttgctggcc caacttcaca gttttagtcg ggatatgtaa
      841 ttcataattc ataaggagtc agccttggta agtgttaatg ctcatcttgt atcggtaacg
      901 aacaaaattt aatagagcag atagatacaa cagttgcaaa agcaataatt aagcgtgtaa
      961 gacgttaatg tttaatgtgt gtatggactc ctatttttga gttttattta agctgtgaaa
     1021 tcaaaagaaa aaacagcaag aaaggtcatg aaaagacaaa ataaagaacc taaattatag
     1081 cctattttta tgcttgtaaa cacaatcacg cagtaataat ttaaaaaaac tacatctaat
     1141 ctatagatag cctaagtttg cgtggtggtc actaccgata aatcggcagt taccaccacg
     1201 cccctacacc agcgatttgc aagcgtgaat aaattcactt tgcccatcgg gtagctctgc
     1261 aatgtatcgt tcaaaaagtg ctattaaatt ttgttcagtt tctccgctgc catagcagcc
     1321 gtttaaaaat ccctttttgc cactccagaa ctcatggcgt ttttatactt aattaaaaga
     1381 tcaaaagtga tctgatcaac ttctcttaaa gtgatacttt cttcatctgt ttcatttgca
     1441 atttcttcat ctgacttctc accaatccca accagttttt gagacctcga gaccaaacca
     1501 attgacgagc acccttcata gaaatagcga attcctgcca taacttactt ggcaatttct
     1561 caaaaatttc ctgatcttca atcgacaact gaagcaaatc aaacggactt aatgattctt
     1621 tacgaccacg ttttacgtga ccttttgtca tctcgtaatc cagcccccat ttcgcaacat
     1681 attcttgagc atacgaacca tcccgaatat ccagtccatg cttcatagat gggcttttca
     1741 atcctttcga ctcacaacaa tgcatccagt attttgcgag tctgtcccta tacgcatagg
     1801 ctaaatcgtc agtatctttg gtcaataaaa gaatatgaaa atgcggatgc catccgtttg
     1861 aaccataagt gacttcaaga ccacggactt tgtattgaat cttgcaaagc ttggataaat
     1921 ctttgaactg ccgaccacca aaaacatagt gatcgcattt tttaacttgc gtaaaaggac
     1981 tttcaacggc tcatcttttg aatgactaaa tgtcaaagtc attaatttaa tatctgaatt
     2041 ttcttgttgt tcccaatatt taattaactg gcccatctca acacgtcgtt cttcagtaat
     2101 ctgcttagca caaactgggc atgaccagac cgatccacag cgttgcaaat tgccccagtt
     2161 agtttttccg tgcttcatta aacatgacag tacggttttt atgtttatcg atacgtcttt
     2221 ttaaacaatt acaaacacgt tcttgcggca aaagttttgc agattgatct tgaagaatat
     2281 agctgtgaag tctagcccct acaccctgtt tatttttgta cagtaaggag gctgaaagcc
     2341 tttgttttcg ggcgtggcct gagatttagt aagtttacca agaccacccg attttgatgc
     2401 agacgagcct tcagaagacg caggaacgcc tttaacaaaa gtgtggtttt cgtgggtttt
     2461 gtgaaattta ctgtaagatt tacactattt ggcatctctc aagtcctttt aatgacttgc
     2521 gagatgtttg cttactgtta taataacttt gcatgctgac attcgctgac ggatcagtgt
     2581 gtacatcaag agcccatcta gtcgccaaac tgaatacggg cttttttgta tctgcaccaa
     2641 tcatattgtt aataaaaatt ctgttatgaa gttacaactg tttcataaac attgcaatac
     2701 tgctgccact ttagcttacc aaaattataa tttttaaagc cttatacata aaaaacttcg
     2761 gaaaaaaggt ttataccttc catacctcca tgtaacctgc aattacgtca ctataaacat
     2821 tggatgacac tatggcgcgt ataacagtta cacaagcatc aaaagacttt aatatcagtc
     2881 gaaacacttt gtataagaag ataaaacaag gacatttgac aaaagattcg aatggattac
     2941 ttgacgttaa tgacttaatt cgtgtcatcg gtgcacacac agaaagtaca tctaaaaacg
     3001 tcactttaaa cactagtgac attcaatctg ttagcagtga cacacagcta caacaggcga
     3061 aaagtgaagt tgaacaactt aaacaattac tagctcaaaa gaaattttca taaaccagct
     3121 acagcagcaa attgcagacc tacgccaaga caaagaacaa ctctacaacc agattaacca
     3181 aaagcggcta gaacataaaa ggggttactt ggcaggtttt ggatagttta cctgttataa
     3241 aaagcagtaa tcagagaagg gaattacccg aatatctctt gtcgctgggt agacacccag
     3301 cttaagccgc caagactggc ggttttatct agagttcagg gacgtgacca gtgaaaaaca
     3361 acaagtccgc tctgttagcg gacttgtggc acaaaagctg cgccgatcat gcagacttag
     3421 gctatctttt tgctttctgc tatctcacct agtgttgcga atgctggttc tcttggccag
     3481 tgttcttctt taattccata atcctcataa aaacacggca ttaatgatag ggctgatctt
     3541 cccttataca agcaaattgc tgtgtgttgc tccaattgtt ggaattttca ggttcaaaga
     3601 ccttaagaat gcctgtgttt ttacttaaag tctttgatcc actgttactt acaatgtcgt
     3661 tattcaacaa aatcgcaggg ttgtgaatgt ttggcgtgct tgtaaaaccc ttaacttcag
     3721 caaaaccacc aattaaagct tcagtttcac caaccattct tgagaatgtt tggcaagttt
     3781 catcatcacc tattctaaag acaaccttaa ggtctgctga tcccattatc agacgttccg
     3841 catcattccc ataaagtcta tttaagccct ctaaactttg gaaatagagg aaggtaggca
     3901 ttttgcagaa cgaatcatat ttaatgtact tgtgaaatta gggattggtg cacagttaat
     3961 aatttcatct agcgcaacaa aaaccgcatc acgattacga caattggttt gtaaaattcg
     4021 taaagtttga gcaaccatcg caccgaatat tgttgctgtt gaactcctaa attcctgctc
     4081 gaactgtaaa aaagtatttg ttttggctga acaagtagtt tttaatgaaa aatcagagcg
     4141 ttgtacttgt tttctaattc tatcgtcatc taagaaagca aacgctttag ataatgatgt
     4201 aaaaatagaa gcgagaaggt tttcattttc gcagtacgtc caatctcaga tgcaattgct
     4261 gatactgaat cattacttga tcttttcaga atttttaaag gtcagcaata ttatcttcgg
     4321 agttaatgaa ttttgagcat ttggaagtga agcttcttcg ccaagctcaa gcaataccgc
     4381 ctttaaaagc cgtctagcat tttctgcaaa tacttctgaa ctgtcatcaa cgtaagggat
     4441 gattacactc aaaatttcat ttaattctgc actactagat gattctgaaa aaagattgta
     4501 agcgtgagaa tgtatatcgg ttggattgaa aaccgtagga gtgtatccgt agcgttcaaa
     4561 aacaccggct tcgagtagtc gtccataaat ttccggcttc acatctgtta agacaacaga
     4621 tctccctgat tgcatccagt caacaacttg agcaatgagg aaagcagttt tacccgaacc
     4681 aggtgcacca actaccatac ctctatcttc attggaaaca cgagctttaa aacgattttc
     4741 agaccataaa tcatcttcca atttatcgcc tagccaaagc aaaggtgctg ttggctcttc
     4801 agtaattgta agcttttcaa aaagcccagc acggttttca atatcaatac gttttcaata
     4861 ttggattcaa ctttattatt ttggttgttg tattccaact tttcttcttt tgaaagaaag
     4921 aagtaaaggt catctcgaat tgtaaggctt ctcaaccaat acaacatata aaatattgca
     4981 ggtaagcccc aaaaaagtag accttcaaca tccaatctaa ggataaatta gtcagcgtaa
     5041 cgaatgcagt tatgataaga gccatgctga caaagaacca aaaccagtac tgcaaaaatg
     5101 actgagcctc agtattacca ttttcaatac gttttctagc ggtagcatga gcaaacttaa
     5161 caacaattga accacttaaa aaacaggtat gaatgaaaaa gtagcacaaa taatatattt
     5221 accaacaccg ctattaaaaa agccattcac aaaatcgcta atcatccttt tgctccatat
     5281 tcttgttttc cttcgcaaat ttccaagaat tgtggctcat taacaggact ttttaaccaa
     5341 gaacaacgaa ccatataata ataagctgaa tccaaagact taaactttat caagtttgaa
     5401 cttgttttta attctgcttt ttcttcccaa accaacaact tacagttaat acaacgacca
     5461 caaagcggca gattcccacg tatctcggat gcaataccca ttatttttac tccatgaccc
     5521 aaggtacacg gacgtaatat ttatacggct atgccctaat tcctcactaa tctgcatacg
     5581 cacctcacca tccttaacct tttctgcctc tgtcagctct cgataaccac caacagcagg
     5641 gcaaggaaag cccattaatt ctaaatatcg tgtttgagca tagccatgtc ttaaaccatg
     5701 tgtttgaccg atctcaaggg cattagtgac cttttcccat gtggctaaat gtgccttata
     5761 cgtttttctg gcggtattaa cgaaccatcc tttgctaaag ccagtgcact cttcagctct
     5821 tgtcgttgct tttcgctg
//
