LOCUS       NZ_CP061545            10676 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS80 plasmid pAS80-2, complete
            sequence.
ACCESSION   NZ_CP061545
VERSION     NZ_CP061545.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092247
            Assembly: GCF_014717695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 21:54:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,827
            CDSs (total)                      :: 3,733
            Genes (coding)                    :: 3,385
            CDSs (with protein)               :: 3,385
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 348
            CDSs (without protein)            :: 348
            Pseudo Genes (ambiguous residues) :: 0 of 348
            Pseudo Genes (frameshifted)       :: 280 of 348
            Pseudo Genes (incomplete)         :: 59 of 348
            Pseudo Genes (internal stop)      :: 27 of 348
            Pseudo Genes (multiple problems)  :: 17 of 348
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061545.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS80"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS80-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="CCH"
     gene            join(10540..10676,1..40)
                     /locus_tag="IC800_RS18740"
                     /old_locus_tag="IC800_18740"
     CDS             join(10540..10676,1..40)
                     /locus_tag="IC800_RS18740"
                     /old_locus_tag="IC800_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000246871.1"
                     /translation="MATVSFNKSFVIESPTAINAIINDLENPRKVEVSARDYNAENAKG
                     IKLLKQRLSNSKR"
                     /name="hypothetical protein"
     gene            76..570
                     /locus_tag="IC800_RS18745"
                     /old_locus_tag="IC800_18745"
     CDS             76..570
                     /locus_tag="IC800_RS18745"
                     /old_locus_tag="IC800_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999987.1"
                     /translation="MNDSFACPQNPDVESFLLNKGKGIRFENSSISRTYLILDGDNGDI
                     LAYFSISFKEITINTESLSGTAVKKLNGMSKTANTLKVYLIGQIGKNSLVKPNPIKLDV
                     ILSEIYNVISQAQALIGGRVIILECENQPKLIALYERHGFKLIETMNGSDSLLTMYTSI
                     I"
                     /name="hypothetical protein"
     gene            complement(585..869)
                     /locus_tag="IC800_RS18750"
                     /old_locus_tag="IC800_18750"
     CDS             complement(585..869)
                     /locus_tag="IC800_RS18750"
                     /old_locus_tag="IC800_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110353.1"
                     /translation="MNQEQFQECMKIWIKPETWHTNHPCDTKRFNQAIQQLMAINGAKL
                     LHPEDFSKQLYIALANEYPKLSQKFISEQVEDASQKYDVISSYLYDIRN"
                     /name="hypothetical protein"
     gene            964..1266
                     /locus_tag="IC800_RS18755"
                     /old_locus_tag="IC800_18755"
     CDS             964..1266
                     /locus_tag="IC800_RS18755"
                     /old_locus_tag="IC800_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110354.1"
                     /translation="MKIRVTRGYIAKYPLEVFYISKDIDCNVIPVAGMMFEDIGLVNAD
                     GQVETVEITKVTINPVENIYYVELKQDTDQVDKTVLEQKFKNMVVDDWEYNEYQF"
                     /name="hypothetical protein"
     gene            1728..1874
                     /locus_tag="IC800_RS18760"
                     /old_locus_tag="IC800_18760"
     CDS             1728..1874
                     /locus_tag="IC800_RS18760"
                     /old_locus_tag="IC800_18760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158640837.1"
                     /translation="MNDFFAMVVTLAKHFKDRPDVLFGLIVVVGAITLIWAGKLKGAIS
                     FGG"
                     /name="hypothetical protein"
     gene            complement(1999..2241)
                     /locus_tag="IC800_RS18765"
                     /old_locus_tag="IC800_18765"
     CDS             complement(1999..2241)
                     /locus_tag="IC800_RS18765"
                     /old_locus_tag="IC800_18765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110355.1"
                     /translation="MSIFAKFLDIEQKYKVRLHEGENFKQALYNDKMTDSDDCIIDKIE
                     LVIKHYPDSKNILLSTYSSDETSEIPFCYAVVVPY"
                     /name="hypothetical protein"
     gene            complement(2277..2846)
                     /locus_tag="IC800_RS18770"
                     /old_locus_tag="IC800_18770"
     CDS             complement(2277..2846)
                     /locus_tag="IC800_RS18770"
                     /old_locus_tag="IC800_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_191110356.1"
                     /translation="MAKLSLTEVSKRFKVSRSTVYRAINEGRLSRGADNQFDLAEVIRC
                     FGEPVIKASPEEESKASVKDDTDLRQLVDFMKREIESYKEREQRYLDQIDRFQLLLGHK
                     ESSEKVSHDTPVTRSNDTPCDTLNNTSETIATQAMKQNETSHDTSLTHLNGAPRDTSET
                     SHDTLKKQPKKRGLFGRVISAVFNDN"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(3753..4139)
                     /locus_tag="IC800_RS18775"
                     /old_locus_tag="IC800_18775"
     CDS             complement(3753..4139)
                     /locus_tag="IC800_RS18775"
                     /old_locus_tag="IC800_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110357.1"
                     /translation="MKAPKGISTVTSSNAHIANEKTHIVNSLNYDKLQKIPINPDLYKK
                     IDKVASKRKIVLADIVREALEFYLNNPNAPYCAELIQKNSKKLIYNINHLIDVKVEKFK
                     TDHGVNRSNQIRRALSAWLIKENF"
                     /name="hypothetical protein"
     gene            4561..4800
                     /locus_tag="IC800_RS18780"
                     /old_locus_tag="IC800_18780"
     CDS             4561..4800
                     /locus_tag="IC800_RS18780"
                     /old_locus_tag="IC800_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110358.1"
                     /translation="MVEKITQIGILVEESLKKEFQAICKAQDKNASQEIRALMREYVKK
                     NRIKKLPDLLAGQQSEDLQQDAWDGVKPVGREEI"
                     /name="hypothetical protein"
     gene            4800..5207
                     /locus_tag="IC800_RS18785"
                     /old_locus_tag="IC800_18785"
     CDS             4800..5207
                     /locus_tag="IC800_RS18785"
                     /old_locus_tag="IC800_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191110359.1"
                     /translation="MKNVIKGIALTTFAVLLTACSKPDISGVWIPERVTKDEIFFDYYI
                     IEKKKDSDRYLLKNVTYRIKGGNSYRPMKLPKLIGGQPEKVLKLIKDNTYCVEGSLQTE
                     CVVYMDGKLDFYNQGTFVKSNKKPPEIPVNQ"
                     /name="hypothetical protein"
     gene            5216..6298
                     /locus_tag="IC800_RS18790"
                     /old_locus_tag="IC800_18790"
     CDS             5216..6298
                     /locus_tag="IC800_RS18790"
                     /old_locus_tag="IC800_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_111818387.1"
                     /translation="MKKFIYIFLIFFSFLGLSSAYAVENYTPGQDLGNPDGSITIPDAS
                     TGDTGKNKVSKEIGNVTQQGMGTLDKLMEAIQSNLEQSLKSSLQNYYQKITAPLIYVFG
                     GFILIWITFQGFKMMLGMAVEFQAVVSNFIIMMIIWSIVFSWDAFYPYIAEIFLEDIPN
                     LISEMTGHDTQSTFTTFVTIVFDVVTESFKNVDMGITNVVSGFFFVLVYLVLLLEAVIL
                     CVIFFLIWTLCKMVIAILVVVAPIFIAAAMFPATRKYATNWLQAVLTPNAIILLTIVTC
                     DLFLSGVITAYDNVVGENGGSNFVVAFVLMITIGLVIGMFYLIPRIAISLVGSGFEASS
                     SGTQGVMGGFKSGMKKLFSK"
                     /name="type IV secretion system protein"
     gene            6457..6930
                     /locus_tag="IC800_RS18795"
                     /old_locus_tag="IC800_18795"
     CDS             6457..6930
                     /locus_tag="IC800_RS18795"
                     /old_locus_tag="IC800_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_111818388.1"
                     /translation="MSNTQTYGNNSGVELFLVIVGIVMVACLFAIWKATKSLGLDMWTG
                     VHTVGYLIAWLTVVIGIYKIGLNVPNKFLIPVLTMMFFACFFPVLNDYALQASTPSFHM
                     VPQLSYLPEESQKMEFGATKITVWWGMWYAKVVYLAIAGLIGVGIQKAFFNDD"
                     /name="hypothetical protein"
     gene            7164..10040
                     /locus_tag="IC800_RS18800"
                     /old_locus_tag="IC800_18800"
     CDS             7164..10040
                     /locus_tag="IC800_RS18800"
                     /old_locus_tag="IC800_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_191110352.1"
                     /translation="MANYHLSVKTGKNVGSSGSRGASHYAYINREGKYKRDDIEQIESG
                     NLPSWAVDAQHFWKAADKYERANGRVYTELEISLPRELNQEQRQELVQQFVEKTLGKNF
                     TYSYAIHTPLASDGEQNPHVHLMFCERKLDGIDRTEQQFFRRYNPKNPELGGAGKDRYF
                     SARIFVSDVRMEWANHANDYMEKLGLDARIDHRSYQAIGTEIQSQNFRADYVSHDQYSI
                     NENIKNIKHQNGETIIEKPSEVLKVLTANQSVFTMRELDRFLVNHTDGEEQYLKAREAV
                     LTCPEMAVLYGKDSVVLSSNELVGIEESISTLIYNANQDSRIQKPRTLIDKLPLNAVRV
                     AETRTFNPEQEKAFYTLTSTDRISLLNGSAGTGKSYVLSAVSEAYKDSDYQVYGIALQA
                     ITAKAIASDCDIPSSTIASFLARYESGNIAINDKTVLILDEAGMVGSRDMQKLLMITEQ
                     HNAQLKLVGDSYQLNAVMAGSAFDHIQKNLDHKNIATLENIQRQKTTEMRKASEYLSKH
                     EVDKALDIYQALGKVKACESHEVALARTIHSWSLDHSESKLILAHSNRDVDELNRMARS
                     VLIKQGKLPAESQKVNTYKGEIDLATGDKIVFKQNNHALNISNGETARIIGFERNAQQQ
                     IKGLMVESDRGGQIKKIDLDSYQHFKHGYANTIHSSQGMTVENAYILASENMNANLTYV
                     ALTRHKGNVELNYSATKFNQEEKIWVRKPDGQYQEKELSAFDNLKRTLGRTEVKNFSTD
                     YSVVQAKDELIKNYLQEHRHSISEKREIYNLNSTFKAMTMPDQHFSKQEIIEEVRNSLR
                     SKAILGDEIVKFNGQMNIAKGEQLQLDQELILKTGLFKKEHFEKDTSLQVVDAYRVKDE
                     PIMKARIKGEIYEIPFDKLHIRYSDKYLTEFRQDTSARFDKRFKSELHSKFNQELQQQR
                     QNQPTQNLNAPNSELKNAPRMKNGPRMS"
                     /name="AAA family ATPase"
     gene            10044..10256
                     /locus_tag="IC800_RS18805"
                     /old_locus_tag="IC800_18805"
     CDS             10044..10256
                     /locus_tag="IC800_RS18805"
                     /old_locus_tag="IC800_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_111818390.1"
                     /translation="MKIEELDDQELYELAQSVIGCRISLRSSGRVPEDDREDLAMQLQS
                     LFELNRAELIQIILHHSERYKRENS"
                     /name="hypothetical protein"
     Typing          complement(2278..2846)
                     /name="rep_cluster_1906"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.419"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7165..10037
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.017"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5277..6295
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.353"
                     /coverage="94.0"
                     /evalue="2.45e-172"
ORIGIN
        1 taagctatta aaacaacgat tgtccaactc gaagcgttaa ttcaagaact tggtgaagat
       61 ggtgtttgcc agttcttgaa tgatagcttt gcctgtcctc aaaatcccga tgttgaaagc
      121 tttttactaa ataaaggcaa agggatacgc tttgaaaatt cttctatttc gcgcacttat
      181 ctaattctag atggggataa tggggatata cttgcctatt tctcaatctc attcaaagaa
      241 attacaatta atacagaaag cctctcagga acagctgtta aaaaactgaa tggcatgagt
      301 aaaacggcta atacattaaa agtatatctg atcggtcaga tcggtaagaa tagtttggtt
      361 aagcctaatc caattaagct agatgtgatt ctaagtgaaa tttacaatgt gatttcccaa
      421 gcccaagctc tcattggtgg acgggtaatt atcttagaat gtgagaatca gccaaaactc
      481 atagcattgt atgagcgtca tggatttaaa ttaattgaga caatgaatgg gtctgattct
      541 cttctaacca tgtatacatc tattatctaa tttttataat aatatcaatt tcttatatca
      601 tataaatagc tactaataac atcatatttt tgactagcat cttctacctg ctcactaata
      661 aatttttggc tcagtttcgg atattcatta gctaatgcta tataaagttg tttagaaaaa
      721 tcctctggat gaagaagttt ggctccattt atagccatta actgctgaat agcttgatta
      781 aaccttttgg tatcacaagg atgatttgta tgccatgtct ctggttttat ccaaattttc
      841 atgcattctt gaaactgttc ttgattcatt ttttaatccc aattaattgt tgaaaataat
      901 atttattaag atagtttata tctatagagt taagttctaa caaccttatt ataaaggaat
      961 tttatgaaaa ttagagtaac aagaggatat atagctaagt atccgttaga agtcttttat
     1021 atttcaaaag acatagactg taatgttatt ccagtggctg gaatgatgtt tgaagatatt
     1081 ggacttgtaa atgctgatgg acaagtcgaa actgtagaaa ttactaaggt aaccattaat
     1141 ccagttgaaa atatttacta tgttgagttg aagcaagata cagatcaggt agataagact
     1201 gtgttggaac aaaaatttaa gaatatggtt gtagatgatt gggaatataa cgaatatcaa
     1261 ttttaaagtt tttctaaaat taacataatg cacgttatgc gaagtcattt aattttttct
     1321 ataagtatca atatcttagt ctaagacgtt ccagtaaaca gcgcactgga acggcttttt
     1381 tgtgatcttt cacgttccac gcctattttt taaacattgt tccacgtttt tgattagata
     1441 ttttgcacaa tcagtcttag atttttctgt tgacagtaca ttggaacatt gcaaaattcc
     1501 ccattttttt ctctttataa ttggtatcgg ctttaaattt tggtcgatat gaaaaataaa
     1561 cggaagtcct tccgccttat actttttttc attctatccg aaaatttaag ggttatttat
     1621 agccttaata ctctcgtatt gtttattagg tgtggaaaac acataacgaa cccatatacg
     1681 tatacgagtg taattaagtt cttcaaagtt ttctggattg aatgaacatg aatgattttt
     1741 ttgcaatggt agtaacttta gctaaacact ttaaggatcg tccggatgtc ctatttgggc
     1801 ttattgttgt tgttggcgct attacgttga tatgggcagg aaaactgaaa ggcgcaattt
     1861 cctttggagg ataactaact taaaaattat ggcacttatt ataatgtgtc atgagacgtc
     1921 tcattttgtc tcattgctta tatagcaatg attacaggtg tatcaaacac tgtctcatga
     1981 cacaggccat tccctaaatc aatagggaac tactacggca tagcaaaatg gaatttccga
     2041 tgtttcatct gatgaataag tagataaaag aatattttta ctatctggat agtgttttat
     2101 aactagctcg attttatcaa taatacagtc atcagaatcc gtcattttat cgttataaag
     2161 ggcttgttta aagttttcgc cctcatgcag tctaacttta tacttttgtt ctatatctaa
     2221 aaatttcgca aaaatgctca tattcattct ctatcttaag taaagggtgt atcacattaa
     2281 ttatcattaa atacagcact tataacacgg ccaaataaac cacgcttttt aggctgtttt
     2341 tttagtgtgt catgagacgt ctcagaagtg tctcgtggtg caccattgag atgtgtcaaa
     2401 gatgtatcat gagacgtctc attttgtttc attgcttgtg tggcaatagt ttcagatgtg
     2461 ttatttaaag tgtcgcatgg tgtatcgttg gaacgtgtca caggtgtgtc atgagacact
     2521 ttttcagagg attctttgtg ccctaataat agctggaacc ggtcaatttg atccaaataa
     2581 cgctgttcac gttctttata agattcaatc tctcttttca taaagtcgac caactgacgt
     2641 aaatcagtat cgtcttttac tgaagcttta gactcttcct caggagatgc cttaattacc
     2701 ggctcgccaa agcaacggat tacttccgct aagtcaaatt gattgtcagc acctcgagat
     2761 agccgtcctt cattgatcgc tctataaaca gttgaacggc tcactttaaa gcgttttgag
     2821 acctctgtaa gtgataattt tgccattcac agtatcctga aaaattcttt aatcgatgat
     2881 ttctaagaaa ttctacaccg aaaaacatac atatgttgat ttgtgctcac aaatcaacat
     2941 atgtatgttt tttataatat tttttcctgc aaaatgaaaa gccctccttg acatacatca
     3001 aggagggctt taaattcgtc ggtacgcagt tatttgatgc taccaacatc aagtaacgaa
     3061 gatcataagg acggcaatcc gttatgattt caccaaaggc ttgtacctga actccatttt
     3121 cacagcaaaa taggtttaga tcaagaccct aaatgataag tcttcgttag ttaagtgttt
     3181 tttttcatca aataagcttc tttcagcaag ttttgcctgc ttctgcgtgc ccgattttcg
     3241 gttggcatgc gttgcatgtg gagggaacaa gtgtcactgg tgcaacgtag cgatagtgaa
     3301 aatcgaaaga ttcgagctaa accgttacag gggaacccca cataagcgga gcgttgagag
     3361 tagttgtata ggtcgattaa gttgcatgtt ggggaaaccc tcgcagagag atcgccatac
     3421 ttcgacaagg agcgcgtggg gagaggtgca aaacctttag tccttgtctg ccatgtgtgc
     3481 aaagcatggt gcggatacga ccgatgcgtg gctccgtcag cccattaggt tcagtcggca
     3541 atacttaggg gatgtgccat ctttttagat ggtcgccctt agggtgagta ttgccgaaac
     3601 tcactcaacg ttcaccatca gcccgagcaa agcgagggat gtgggatacg ttgaggatga
     3661 gttgagtgcc ttttcctttt gcaatatgta tgccaaatat ttgatttata ttacttaaat
     3721 taaaaaattt attatttgct taggcttttt atctaaaaat tttctttgat caaccatgct
     3781 gataaagccc gtctgatctg attgctacgg ttaactccat gatcggtttt aaatttttcg
     3841 acctttacat caattaagtg attaatgtta taaatcaatt tcttagagtt cttctggatc
     3901 aactctgcgc aatagggtgc attaggatta ttcaagtaaa attccaatgc ttcccgtact
     3961 atatcggcca atacaatttt tcgtttactg gcaaccttat caatcttctt atacagatca
     4021 ggattgattg gaattttttg caacttgtca tagttcaacg aattaactat atgagtcttt
     4081 tcatttgcta tatgagcatt gctacttgtc acagtggaaa tgcctttagg agctttcatt
     4141 aaatggcttc ctcacgatca agaattttat caatcaagat gtttcttgag gttcttgatt
     4201 aagaatattt caaaaaaact actttagata attcaaaatt acctatctaa atcgctctat
     4261 tttgacgtat ttcgagtttt ttaaactttc ctatagtttc ttagctctct aacttaaaag
     4321 tgtcttaaaa cgcaaaacag tgcgttttag agtttattta aattcttctt gatgccgatt
     4381 tttctgcgta gccatgcttt gcattggctg tactcgaaaa atagccatct ccatttatac
     4441 atatattgat ttgtactcac aaaacaattg atttacaagg gtttttttca aaattaatac
     4501 aaaaattagt tctcattaaa attatatttt ggcctgagtt aaatgaaatt ttgaggatgt
     4561 atggtcgaga aaattacaca aattggaatt ttggttgaag aaagccttaa aaaagaattt
     4621 caagcaatct gtaaggctca ggacaagaat gcaagccaag agatcagggc attaatgcgt
     4681 gaatacgtta aaaaaaatcg tattaaaaaa ttaccggatt tattagctgg gcagcaatct
     4741 gaagatttac agcaagatgc ttgggatggt gtaaaacctg ttgggaggga agaaatataa
     4801 tgaaaaacgt aataaaaggg attgccctga ctacttttgc tgtattgctt acggcttgtt
     4861 ctaaacctga tatttcaggt gtctggattc ctgaaagagt gactaaagat gagatttttt
     4921 ttgattacta cataattgaa aagaaaaaag attctgatag atatttactt aaaaatgtga
     4981 cttatcgaat caaaggagga aactcttatc gaccaatgaa attacctaaa ttgattggtg
     5041 gtcaacctga aaaagttctg aaacttatta aagacaatac ctactgcgta gaaggttcat
     5101 tacaaacgga atgtgtggtt tatatggacg gaaaactaga tttttataat caaggtacat
     5161 tcgttaaatc aaataaaaaa ccgcctgaaa ttccagtaaa tcagtaggtg ataccatgaa
     5221 aaagtttatc tacattttcc tgatcttctt tagttttttg ggccttagtt cagcttatgc
     5281 agttgaaaac tacacaccag ggcaggacct tggaaatcct gatggatcta taacaattcc
     5341 ggatgcttca actggcgata caggaaaaaa caaagtatca aaagagattg gtaatgttac
     5401 ccagcaaggt atgggaacat tagataaatt aatggaggca atacaaagta atttagagca
     5461 gagcctgaag tctagcttgc aaaattatta tcaaaaaatt actgcaccac tcatttatgt
     5521 ttttggtggt ttcattttga tctggattac atttcaaggt tttaaaatga tgcttgggat
     5581 ggccgttgaa tttcaggctg ttgtttccaa ttttattatc atgatgatca tctggtcgat
     5641 tgtatttagt tgggatgcat tctacccata catagcggag atatttcttg aagatattcc
     5701 aaacctaatc agtgaaatga cggggcatga tactcaatct acatttacga cttttgtgac
     5761 aattgtattt gatgtcgtga cagagtcttt taaaaatgta gatatgggaa ttaccaatgt
     5821 cgtatctgga tttttcttcg ttttggtcta tctcgttcta cttcttgaag ccgtcatctt
     5881 atgtgtaatt ttctttctaa tctggacatt atgcaaaatg gttattgcca ttttggttgt
     5941 agtcgcacca atttttattg cggcagccat gtttcctgct actcgtaaat atgcaacgaa
     6001 ttggcttcaa gctgtactta cccctaatgc tattatttta ttaacaatcg tcacatgtga
     6061 tttgttctta tctggagtta taacagcata cgataatgtt gttggggaaa atggcggatc
     6121 taattttgtg gtcgcttttg ttttaatgat tacaattggc cttgtcatcg gaatgtttta
     6181 tttgattccc cgaattgcta tctctcttgt gggaagtgga tttgaagcat ctagttctgg
     6241 tacacaaggt gttatgggtg gttttaagtc aggaatgaaa aaactttttt cgaaataata
     6301 acccgagata aatagagcta agtgttaacg aagcgaactg ataattttaa ttttttaccc
     6361 tctttgatta ctgttttact tcgagcgaag ccgtcaaaat ctgtgatttt ggcgagctga
     6421 ccgaagaagt aaaacctaat aattaggatt gataatatga gtaatacaca aacctatgga
     6481 aataatagtg gtgttgaact atttctagtt attgttggaa tagtgatggt tgcttgcctt
     6541 tttgcaatat ggaaagcaac gaagtcatta ggattagata tgtggacggg cgtacatact
     6601 gttgggtatc taattgcatg gcttaccgtg gtgatcggta tttataaaat aggattaaac
     6661 gttccaaata agtttttaat ccctgtatta acaatgatgt tttttgcttg tttcttccct
     6721 gtgttaaatg attatgcatt acaggcttct actccaagtt ttcatatggt gccacagctt
     6781 tcttatcttc ctgaggagtc tcaaaagatg gaatttgggg caactaaaat tacggtctgg
     6841 tggggcatgt ggtatgcaaa agttgtttac cttgcgatag ccggattaat tggtgttggg
     6901 atacaaaaag cattttttaa tgatgattaa tctgcctgtt caataaaatc ttgtatttaa
     6961 aaccacatta aatatgtggt ttttttacgt ctatagaaaa atttctaata aaaaacagtt
     7021 gcctatgttt taaagaaacc ggttacgaaa ttcaaatact ggaattggat aggtgagggg
     7081 tatcattacg agtagcaatg agtaatgcac gcttccgcgt ttctctccga gaaaccaaaa
     7141 agcggttatc gaattagggt aaaatggcga attatcattt atcagttaaa accggtaaaa
     7201 acgtaggttc ttcgggaagt cgaggggcaa gccattacgc ctatattaat cgtgaaggta
     7261 agtataaaag agacgatatc gagcaaatcg aatcaggtaa cttgccatca tgggcagtag
     7321 acgcccagca tttttggaaa gcagcagata aatatgaacg tgccaatggc cgggtctata
     7381 ctgaattaga aatctctttg cccagagaat taaaccaaga acaaagacaa gaactggttc
     7441 agcaatttgt agaaaagact ttagggaaaa actttacata ttcttatgcg attcatacac
     7501 ctttagccag tgatggtgaa cagaatccac acgtgcacct gatgttctgt gagcgaaaat
     7561 tggatggtat tgatcgtaca gaacagcaat ttttcagacg gtacaatcct aagaatcctg
     7621 aattaggtgg tgcagggaaa gaccgctact tctcagcaag aatattcgtt tcggatgtcc
     7681 ggatggaatg ggcaaaccat gcaaacgact atatggaaaa attgggatta gatgcccgaa
     7741 tcgaccaccg cagttatcaa gctattggta ccgagatcca gagccagaac ttcagggctg
     7801 attacgttag tcatgatcag tacagtatta acgaaaacat taaaaatatt aagcatcaaa
     7861 atggtgaaac cattattgag aaaccgagtg aagtgttgaa agtgctcacg gccaatcagt
     7921 ctgtttttac catgcgtgaa ctcgatagat tcttggttaa tcatactgat ggtgaggaac
     7981 aatatttaaa agcccgtgaa gctgttttga cgtgccctga aatggcagtt ttatatggta
     8041 aagacagtgt tgttttatct tcaaatgaac tggttggaat tgaagaatct atatcaacac
     8101 tgatctacaa tgcgaaccaa gacagccgga tacaaaaacc aagaacactc attgataaat
     8161 tgccattaaa tgcagttcgg gttgctgaga cacgaacgtt taatccagag caggaaaagg
     8221 cattttatac actaacttcg acagaccgga tcagtttatt gaatggttca gcaggtacag
     8281 gaaaatccta tgtattgagt gctgtatctg aagcttataa ggattctgat tatcaagttt
     8341 atggtattgc acttcaggcc atcacagcaa aagcgattgc ttcagattgt gatattccga
     8401 gcagtacgat tgccagtttc ttggctcgat atgaaagtgg aaatatagca attaatgata
     8461 agactgtatt gattctggat gaagccggta tggtcggttc cagagatatg caaaaactat
     8521 tgatgattac tgaacagcac aacgcccaac ttaaattagt gggtgatagc taccagttaa
     8581 atgcagttat ggccggttca gcgtttgatc atatccagaa aaacctagac cataaaaata
     8641 tagcgaccct agagaatatt cagcgtcaaa aaaccactga aatgcgtaaa gcttcagaat
     8701 atttatctaa gcatgaagtt gataaggctt tagatattta tcaggcttta ggcaaggtca
     8761 aagcatgtga atcccatgag gttgccttag ccagaacaat tcattcatgg tctcttgatc
     8821 acagtgaaag taagctgata cttgctcact caaatcggga cgtggacgaa ttaaacagaa
     8881 tggcccgttc cgtcctgatc aagcaaggta aactccctgc agaaagccaa aaggtaaata
     8941 cttataaggg tgaaatcgac cttgcgactg gagacaagat cgtattcaaa cagaataacc
     9001 atgctttaaa catctccaat ggtgaaacag ccagaattat tggctttgag cgtaatgccc
     9061 aacagcagat taaaggcctg atggttgagt cagatcgggg cggccagatc aagaaaattg
     9121 atttagatag ctatcagcat tttaagcatg gttatgcaaa tacaatccat tcctcacaag
     9181 gaatgaccgt agagaatgcc tatattcttg ccagtgagaa tatgaatgcc aatctcacct
     9241 atgtagcttt gacccgtcat aaaggcaatg tggaactgaa ttacagcgcg actaaattca
     9301 atcaggaaga aaaaatctgg gttagaaagc ctgatggcca atatcaggaa aaagaactgt
     9361 cagcattcga taacctgaag cgaacattag gccgtacaga agttaaaaat ttctctactg
     9421 attattcggt ggttcaggct aaagatgaat taattaaaaa ctatcttcag gaacaccggc
     9481 acagcatttc agagaagcgt gaaatctata atctcaatag cacgtttaag gcaatgacta
     9541 tgccagatca gcatttcagc aaacaagaga ttattgagga agtccgaaat agcctaagaa
     9601 gcaaggccat tttaggggat gaaattgtta aatttaatgg tcaaatgaat atcgccaagg
     9661 gtgaacagct ccagttagac caggaactca ttttaaagac cggtttattc aaaaaagagc
     9721 actttgagaa agatacttcg cttcaggtcg tagatgccta tagggttaaa gacgaaccaa
     9781 ttatgaaagc tcgaattaag ggagagattt acgaaatccc gtttgataag ctgcatattc
     9841 gttattcaga caaatatttg acagagttca ggcaagatac gtcagcacgt tttgataaac
     9901 gctttaagag cgaattacac agcaagttta accaagagct acagcagcaa agacagaatc
     9961 agcccacaca aaacctaaat gcaccaaaca gtgagctgaa gaatgcccca cgtatgaaaa
    10021 atggcccaag gatgagttag gtcatgaaaa tagaggaact ggatgatcag gaattatatg
    10081 agctagcaca gtctgttatt ggctgccgga ttagcctaag aagttcagga agggttcctg
    10141 aagatgatcg agaggatctc gcgatgcaac tacaatcatt gttcgagttg aatagagctg
    10201 agttaattca aattattctg catcatagtg agagatataa aagagaaaat tcataagtta
    10261 ttgtttttaa atttattatt tagataaaaa agccctatat tgttttgtat tcacaaaaca
    10321 atatagggct ttttctcgat gcattgattg tttcgtgggt acaaaaaata aataaataca
    10381 tttttattat ttttcatatg tttacaattg aaaatgtaaa atagaatttt acttttcaat
    10441 agaaaatatt tactattgag aactaaatga acattcgtga agtttaatga actttagtga
    10501 atatttattt tactgccaga actagaggag taccgtgtaa tggctactgt ctcgtttaat
    10561 aaaagctttg taattgaaag ccctacagca attaatgcta taatcaatga tctagaaaat
    10621 cctcgcaagg ttgaggtttc tgctcgtgat tacaatgccg agaatgcaaa gggtat
//
