LOCUS       NZ_CP061402             9294 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain NU-CRE085 plasmid pCRE-085-3, complete
            sequence.
ACCESSION   NZ_CP061402
VERSION     NZ_CP061402.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14453493
            Assembly: GCF_012029975.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Krapp,F. and Hauser,A.R.
  TITLE     Carbapenem-resistant Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Krapp,F. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2020) Medicine, Northwestern University Feinberg
            School of Medicine, 645 N Michigan Ave, Suite 900, Chicago, IL
            60611, USA
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Lopez,F. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2020) Medicine, Northwestern University Feinberg
            School of Medicine, 645 N. Michigan Ave., Suite 900, Chicago, IL
            60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-AUG-2020
            Assembly Method :: Flye, Unicicyler v. 2.7.1, 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 470.0953392789152x
            Sequencing Technology :: Oxford Nanopore MiniION, Ilumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:19:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,671
            CDSs (total)                      :: 5,547
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 49 of 131
            Pseudo Genes (incomplete)         :: 88 of 131
            Pseudo Genes (internal stop)      :: 14 of 131
            Pseudo Genes (multiple problems)  :: 19 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061402.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NU-CRE085"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE-085-3"
                     /geo_loc_name="USA: Chicago, IL"
                     /lat_lon="41.8945 N 87.6208 W"
     gene            join(8839..9294,1..75)
                     /locus_tag="HCV82_RS28095"
                     /old_locus_tag="HCV82_028090"
     CDS             join(8839..9294,1..75)
                     /locus_tag="HCV82_RS28095"
                     /old_locus_tag="HCV82_028090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(103..282)
                     /locus_tag="HCV82_RS28100"
                     /old_locus_tag="HCV82_028095"
     CDS             complement(103..282)
                     /locus_tag="HCV82_RS28100"
                     /old_locus_tag="HCV82_028095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            351..728
                     /locus_tag="HCV82_RS28105"
                     /old_locus_tag="HCV82_028100"
     CDS             351..728
                     /locus_tag="HCV82_RS28105"
                     /old_locus_tag="HCV82_028100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(790..1089)
                     /locus_tag="HCV82_RS28110"
                     /old_locus_tag="HCV82_028105"
     CDS             complement(790..1089)
                     /locus_tag="HCV82_RS28110"
                     /old_locus_tag="HCV82_028105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1079..1342)
                     /locus_tag="HCV82_RS28115"
                     /old_locus_tag="HCV82_028110"
     CDS             complement(1079..1342)
                     /locus_tag="HCV82_RS28115"
                     /old_locus_tag="HCV82_028110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(1648..2067)
                     /locus_tag="HCV82_RS28120"
                     /old_locus_tag="HCV82_028115"
     CDS             complement(1648..2067)
                     /locus_tag="HCV82_RS28120"
                     /old_locus_tag="HCV82_028115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2470..4155
                     /locus_tag="HCV82_RS28125"
                     /old_locus_tag="HCV82_028120"
     CDS             2470..4155
                     /locus_tag="HCV82_RS28125"
                     /old_locus_tag="HCV82_028120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4165..4422
                     /locus_tag="HCV82_RS28130"
                     /old_locus_tag="HCV82_028125"
     CDS             4165..4422
                     /locus_tag="HCV82_RS28130"
                     /old_locus_tag="HCV82_028125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            4507..4656
                     /locus_tag="HCV82_RS28135"
                     /old_locus_tag="HCV82_028130"
     CDS             4507..4656
                     /locus_tag="HCV82_RS28135"
                     /old_locus_tag="HCV82_028130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6137..8101
                     /locus_tag="HCV82_RS28140"
                     /old_locus_tag="HCV82_028135"
     CDS             6137..8101
                     /locus_tag="HCV82_RS28140"
                     /old_locus_tag="HCV82_028135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            8101..8832
                     /locus_tag="HCV82_RS28145"
                     /old_locus_tag="HCV82_028140"
     CDS             8101..8832
                     /locus_tag="HCV82_RS28145"
                     /old_locus_tag="HCV82_028140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     BGC             1..9155
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4760..5338)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8102..8829
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tattttaacg tgccggtacc tgaactgccg gaggacaaac ctgcccgccg gtggtggccg
       61 ttcggcaaaa aataaatgcc agcaccggcc atgccggtgc tgttattcct ccccgagctt
      121 atcccgcatc acctgccaga gtgtttccgc atcctcgtgt agccgctcgc acatgtcggc
      181 cggggtatcc atatccagct cattcagttt atcgagcaat agcagcgact gctccttaat
      241 aaatttagcc atattgaggg ctgttttttc ctgttttgtc atgattcttt ccttccgggc
      301 atggtgtgcg cctgtcaggt atactgtctc aaaaatagcg gggtgagaca atggcgtggg
      361 tgacggtgag acaggcgacg gaactgacgg gtaaagcccg aagttccctg tatcgggata
      421 tggcgaaagg ccgcgtatca taccgtacgg aagctgatgg cggccgggtg gtggatacca
      481 gcgagcttat ccgggtgtac ggtgagttaa gacaggatga gactcacgag cgggacgagt
      541 tgagactgtc gggtgagact gaaaaaatgg cctccgacgc gttgatcgcg gagataaaag
      601 cactcaggga ggaggtggcc gggctacggc aggaaatgca gtccatgaga ctgctggaac
      661 acaaaccgga gacagcagag accccggccg gaacaccgcg ccggtggtgg ccgtggggaa
      721 aacgctgacg cgctgtgctt gtccaaccgg cgacggccac gcaatgaatt gcgtccccgt
      781 caccggttat tagagaacaa tatggggatt ctggcgctgg ccgaccacgg ccagaacccg
      841 gacttttctg gtctggtcat caacatcata gaggacatgc tgtcccatca gactgatccg
      901 gcgaacgtcc tcgccatagc gtgagtccac ttcatagagt cgcgggaagt cggccagttt
      961 gtccgccgca gcgagaaccc ggtcggtaac gttctgcgca tcccacaggg atgaattttc
     1021 cgcgatatag tcaaacagcg tcgttacatc ctcgatggct ccctgcttcc attccacgtc
     1081 atacggcatc gcgcagttcc tgataacggc catgcaggcg ctggcggagt tgttccgtgg
     1141 tcatacccgg acgcgtgtca gcacgggcgg cggtcacttt ggcgcgaacc tgatcttcat
     1201 actctgcctg ggccagttct tcgttagcca gtcgctgcaa cagcgcagct ttgtccgggc
     1261 caccttccgg cagtatgcgg ggcaggcttt cagccagttc ccgaagaaag ttggcgtttt
     1321 cgtagtgtgt agtgttgctc attgcggagc ctcccgggtt aatgcggcaa tatgaaggaa
     1381 gtttagcagg gggcggtctg aaagaatatg gtcgtgtcag tcaggctgat gaagcagaaa
     1441 gtcggtaaaa aagggcttac ggcatccatt ttacgtcaaa acatatgcca tgcagttgcg
     1501 tgcataggca tgcattagga taaaatttat cgggcgcgtt tccggcagtt ttccgggggg
     1561 gttgttgcct gttttatccc gtagccgccg gaaacgccct gagcccgtct gagcggtgcg
     1621 cgtaatgacg cgttatggta aatccactta ctgtctgcct tcatagccat cgagataaac
     1681 agctgaaatg gtgtcagcca gtagccttga ttttttcggt gtgaggtctt tgctgcggta
     1741 atcggtaccc gcaaattgtg ttgtgccttg gagtgagttt aaaaattcct gactgtgaaa
     1801 ctgctgaatg cgttgctgca catcactccg tgattttccg gtcgctttat cctttgcgcc
     1861 catatcccag aactgccgga aaaaaggcac catcattcgt gttgtgtcag ctttctgggt
     1921 ggaaaaattg ggatcaaaac cgtcatctga agcatatacg taatgccggg tatgctgttc
     1981 cacgctgccc tttggagcac aggcagtgag agttgctgtc gctgttatca tggcgataag
     2041 acacagtttg ctgccggaac gtctcatgag cgtctcctta cctttaattc ttttttctca
     2101 ttgagttata tatccgcagg ctatcttatc atcagtttct gaccgtgttc agtgaccgaa
     2161 caacccgaac cagacccccc gtttcttttc ctgtcccgga gggggctttc tggtatctca
     2221 tataatgatt tcagtaaaac atactgaggc cggagaacgg cgccgtttgt gctggttttt
     2281 gttgcatctg gaggggattg cgcatggtgt gagtggctgt ttttttgatc attttcacaa
     2341 aaacgcaaat ttacagactt gacatgaaaa acaaggcagc gtaattttta tactgtatat
     2401 aaaaccagtg gttatatgta cagtatttta ttttagttta ttgttttaaa agtcaaagag
     2461 gaaaagtata tgagtggcgg agacggacga ggtccgggta attcaggtct gggacacaat
     2521 ggtggtcagg ccagtgggaa tgtgaacggt acatcaggta aaggtggccc ttcatcaggt
     2581 ggtggtacgg atccaaacag cgggccgggc tggggtacga cgcatacacc taacggggat
     2641 attcataact ataacccggg ggagtttggt aacggcggga gtaaacccgg tggtaatggc
     2701 ggtaacagcg gcaatcatag cgggagctct ggtggcggac agtcttcggc caccgcgatg
     2761 gccttcggtc tgcctgcctt ggccactccg ggggctgaag gactggcttt atccgtttcc
     2821 ggcgatgcgt tgtcggccgc tgttgctgat gtgctggctg ccctgaaagg tccgtttaag
     2881 tttggtctgt gggggattgc gatttacgga gtgctgcctt ctgagatagc aaaagatgat
     2941 ccgaaaatga tgtcaaaaat cgtgacgtca ttgccggcag acacggtaac ggagacgccg
     3001 gtcagctccc tgccgctgga ccaggcgacg gtcagcgtca ctaaacgtgt ggcggatatt
     3061 gtgaaggacg agcggcagca tattgcggtt gtcaccggcc ggccaatgag tgttcctgtg
     3121 gtggatgcga aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg
     3181 tctctgcagg tgagcgtacc taaaggtgtt ccggccgcga aagccccgcc aaaaggcatt
     3241 attgctgaaa aaggtgattc acgtccggct ggttttacgg ccggtggtaa ctcccgtgaa
     3301 gccgttattc gtttcccgaa agagagcgga cagaaaccgg tctatgtgtc ggtgacggat
     3361 gttcttactc cggcacaggt aaaacagcgt ctggaggaag aaaagcgtcg ccagcaggca
     3421 tgggacgctg ctcacccgga agaggggctg aaaagagagt atgataaagc gaaagctgag
     3481 ctggatgctg aagataaaaa tattgcgacc ttaaacagtc gcattgcatc gacagagaag
     3541 gcgatccccg gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa agaggcagag
     3601 gcaaataaag atgattttgt gacttataac ccgcctcatg aatatggctc cgggtggcag
     3661 gatcaggttc gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagctgcc
     3721 cagacatctt taaacgaaat gaatgaatcc ttatccaggg ataaggctgc gctttccggg
     3781 gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taaattaaat
     3841 gaggaaaaga aaaaacctcg taagggaact aaagattacg gccatgatta ttttcctgat
     3901 cccaagactg aagatattaa ggggttggga gagttgaaag agggtaaacc taaaacccct
     3961 aaacaaggtg gtggtggtaa gcgggcccga tggtatggtg ataaaaagcg taaaatttat
     4021 gaatgggatt cccagcacgg tgagcttgaa gggtaccgcg ccagtgatgg cgaacacctc
     4081 ggggcattcg atccaaaaac gggtaagcag gttaaagggc cggatccaaa acgaaacatt
     4141 aaaaaatatc tttaagaggt aaatatgggg cttaaattac atattcattg gtttgataag
     4201 aaaaccgaag agtttaaagg tggtgaatac tcaaaagact tcggtgatga tggttctgtc
     4261 attgaaagtc tggggatgcc tttaaaggat aatattaata atggttggtt tgatgttgaa
     4321 aaatcatggg tttcgatatt acagccacac tttaaaaatg taatcgatat tagtaaattt
     4381 gattactttg tatcctttgt ttaccgggat ggtaactggt aagcaaaagt tatcaaggat
     4441 gatttgaaat acagacttcc ggtgttcagg gatgaatacc ggggttttta aatacaggga
     4501 aggtatatga aaaaggtaaa ggttgttttt ctttttatat tgattttttc aggctttctt
     4561 ctggtcgcat gtcaggcaaa ctatatccgg gatgttcagg gtggaacggt ggcaccatcg
     4621 tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag
     4681 atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg
     4741 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag
     4801 agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc
     4861 aaccgcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca
     4921 gtggcgtttt gcgtgctttt ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca
     4981 gcagtcgggc tgaacggggg gttcttgctt acagcccagc ttggagcgaa cgacctacac
     5041 cgagccgaga taccagagtg tgagctatga gaaagcgcca cacttcccgc aagggagaaa
     5101 ggcggaacag gtatccggga aacggcaggg ttggaacagg agagcgcaag agggagcgat
     5161 tcatcggaaa cggtgatgat ctttaagtcc tgtcgggttt cgccactcct gactgattca
     5221 tggttgagcc accggctccc acagatgcac cgaaaaagcg tctgtttatg tgaactcagt
     5281 caggagggcg gagcctatgg aaaaacgcca cctgcgtggc cttgccgttt tgcctcacat
     5341 gttagtcccc tgcttattca cggaatctgt gggtaacttt gcatgtgtcc gcagcgcccg
     5401 ccgcagtctc acggccggag cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc
     5461 acctacaaga cggacaccgg acgcgctgat gtggcatatt gtcccctgat taagtcttta
     5521 tattccctga taataaatta ccaggatagg tgaatcatat cggtttttcg ctgaacattt
     5581 tacgttaacc tatctctgtt gcttttttct ccttacggag aaatacaggt catccccgcg
     5641 agggcgtggc tgacctcagg gggatatccc ctgcttacgc tggctggacg ccaccgacgc
     5701 acggtctctc cccctctttc tggctccgtc cggctggacg ccggccacgc cagaaattca
     5761 cccggccccc gtcaggctca cgcctgacca cccacaaaaa tccacggtgc tggacgcacc
     5821 tggcgaagca gcaaagctgc tcctgatttt gcgggtggtc agccgctcgc tatggcgaac
     5881 tctgacgggg gccggaacac cggaaaagac cgcactgggg tgccgccttt tcctctcaca
     5941 cgctggggcg tgctcgttgt tcctccgggg ctgcgccttt tccgcatggg gcggaaagag
     6001 gctggcctcc gtggtgaaag ctgaacgcaa tcaccacgga cgtgaccgcc gccgccatgc
     6061 cttggggcat gtccgcggct tgtgcagcgg gggctgcaca tcacgccggc aaaccatcgt
     6121 ctgaaggaga gagaacatgt ttaatacgga ttcgcttgcc tggaagtggc acattccgga
     6181 gctggcccgg cagatttatc cgtgggtcag ctggctgtac caggagtatt acggtctggc
     6241 cggggctttc ctagttatcg gcctgttttt cggtctggct gaactttggg ccggacggat
     6301 gcgttttgtg gggctgagtc ataaggagcg acgggagtat gccgatcgct ataagcgcga
     6361 taataaacgg ccagcgccat tttcggtgct ggcctcgctg gattcgcccg ggcggatggt
     6421 cagggcacgt cgacatgtct ggaactacgc gtactggctg accggcgcgg ccggtctggc
     6481 ccttctgggg gggctggttg tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg
     6541 tccggacctc tacgcaaacg cagattatta cctgctgcag ccggctaacc tgttcgggat
     6601 tgcctggtgc gcgggcgggc tggctgtcgg gattgtgttg gcgcggctgc tggcgttcct
     6661 gctgcttgac ggtcattttg cggctgctga cgaggcggtg aacgcgaaac tgaatcagga
     6721 gagccggcgc agcagccagc ggacaggaga gatgaccgac gtgcgccacc tgcatttcgg
     6781 ggagccggta ccggttaatg cgctggccga tttttcctcg gagcaggccc ggcaggagca
     6841 ggcggtgttt ctgggtaagg atgagcaggg caagccggtg ctggttccgc gtgatacctg
     6901 gcgcaagacc aacattcaga tcctcggcct gcccgggagc ggcaagagcg tgatggccac
     6961 caacgcgctc attcgctgcg tacgcgattt cggggacgcg gtggtgtact tcgaccccaa
     7021 aggggacgca tgggcgccgc acgtcttccg ggcgcactgt ccggacttca ccctgctgga
     7081 tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc gatctcgacc agtacgcgct
     7141 gaaaaacctg ctgattgccg ggcttaacct gggcgagacc ggcgacgtgg cggaccacta
     7201 ccgcatcagc gagcagaaag cggcgaagct gattgcggag cagtttccgc agggggcaaa
     7261 cattcagcag gtgctggcgg cggcgtacgc gctgccggag gccctgaaaa aggacgtgaa
     7321 ggggttgatc actaggctgg aaaacgtcgc tgacctgagc gtcctgcaga cggacagtgg
     7381 catcgatgtg gcggggatta tcaacggcgg cggctgcctg tacgttatcg gctcgatgga
     7441 cgacgaggcg gtgatccgtg tgcaaaaaat gctgtttgcc cgctgcgcgc aaatcatcat
     7501 tgcgcgggac gagttccggg agtggccaca cgtcagcgtg atgctcgatg aaattaaata
     7561 tctgctgtcg aagtatgtac tgaacgcact ggggacgctg cgcagccggg actgcaatct
     7621 gttgctcgcg caccagtcgc tgggcgattt cgggcagtgc gggcaggatc tgcccgctga
     7681 atttgtgaaa accacggtac tggataacac gccgatccgc tggttttacc gggcggccag
     7741 cctggagtcc gcgcagtggg cggcggggca gaccggggaa atcagggtgg acgtggagcg
     7801 ccgccgtgcc agccgggagg cggggaacgt ggagcatatc agcggggaca gcttcatcca
     7861 gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa cacctgccgg acgggttcgc
     7921 ggtcatgacc gggctgggtg tggcccggat ggcgttcagc agtccgctgc gggttgagcg
     7981 ccgggaaatc ccgttgcagc gtttcccggt gctggcgaaa accgatcccc tggcggagtt
     8041 ccggcccccg gccgacggcg gcgggccgaa agcggatgat gattttgcgg ggatttactg
     8101 atggcgctgg agctgtacaa cgtcagccac gcgaaacgac aggcgcggaa cgcggagaaa
     8161 acccggctga ccctgcgctg gttgcgggaa gagctgtgtt ccacggcgga gctggttgcg
     8221 cggcggctgg ggattgcggc ggtgcagccg gtctaccgct tcctcgacag cctggtggcg
     8281 aaagggctgc tggtccggac caaatatccg gtggacggcc gtcaggtcag cgtgtggggg
     8341 ctgacgccgc atggcgtggc gttctcgttc gacgaggacg aaccgctggc ggatgttatt
     8401 ccgtttcagc cgtcaagggt gtcggcagca catctgccgc accggctggc cgtgcaatcc
     8461 ctgcgccttg atatggaagc cagaggggcc accggctggc gcattctgca ccgtctgagt
     8521 cttaaaggca tgaaagtgcc ggacgccctg gcggagctgg acggtaaaac ggtggcgttt
     8581 gaggtggagc gcaccgtcaa gagccgtcgc cggtatcagg aagtggtgtc cgggtatctg
     8641 ttcaaccgca aagctaacgg catcgatgaa atctggtaca tctgcccgga ccgggcgacg
     8701 caggtcaggg tgcagcgggc aatcctgtcg gtggatgaaa tcgtgaaccc acagaccggc
     8761 gaggcccgca aaacggcaga gctggatcgt gaacggttgt ttgcgtgctt taaatttatg
     8821 acaacggagt gagggattat ggcgctgtac atggtattta ttgaagacgc ggaggaccgg
     8881 aacggcgagc gctgcatcat gccgaccagc gagcatctgg aagatatggt ggtgctcgcc
     8941 tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg cccagcatct ggcgatgatg
     9001 aaatccatcg ggatgtatga cgagcggctc aacagcgacg aaaaagccac gtactttttc
     9061 actggtaaaa atccgcaatg ggaacggaac aaacccacag gaatgaagct gtgtgattac
     9121 ctctatcgca aggtggagcg gttcgccatc gagaacggtg tgcaggtggt tgatgccggg
     9181 aagtgctcac cggagcagat taacgcgatg attgacgtgg tgagtgagcg gcacggggaa
     9241 agcgcggtga acgagaagct gaaagcggag ctgatgcggg aatataacag ccgc
//
