LOCUS       NZ_CP061395             9294 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain NU-CRE195 plasmid pCRE-195-6, complete
            sequence.
ACCESSION   NZ_CP061395
VERSION     NZ_CP061395.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14453495
            Assembly: GCF_012029695.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Krapp,F. and Hauser,A.R.
  TITLE     Carbapenem-resistant Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Krapp,F. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2020) Medicine, Northwestern University Feinberg
            School of Medicine, 645 N Michigan Ave, Suite 900, Chicago, IL
            60611, USA
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Lopez,F. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2020) Medicine, Northwestern University Feinberg
            School of Medicine, 645 N. Michigan Ave., Suite 900, Chicago, IL
            60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-AUG-2020
            Assembly Method :: Flye, Unicicyler v. 2.7.1, 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 111.46307802652117x
            Sequencing Technology :: Oxford Nanopore MiniION, Ilumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:27:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,755
            Genes (coding)                    :: 5,608
            CDSs (with protein)               :: 5,608
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 72 of 147
            Pseudo Genes (incomplete)         :: 84 of 147
            Pseudo Genes (internal stop)      :: 20 of 147
            Pseudo Genes (multiple problems)  :: 27 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061395.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NU-CRE195"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE-195-6"
                     /geo_loc_name="USA: Chicago, IL"
                     /lat_lon="41.8945 N 87.6208 W"
     gene            complement(1331..1480)
                     /locus_tag="HCV84_RS29135"
                     /old_locus_tag="HCV84_029135"
     CDS             complement(1331..1480)
                     /locus_tag="HCV84_RS29135"
                     /old_locus_tag="HCV84_029135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1565..1822)
                     /locus_tag="HCV84_RS29140"
                     /old_locus_tag="HCV84_029140"
     CDS             complement(1565..1822)
                     /locus_tag="HCV84_RS29140"
                     /old_locus_tag="HCV84_029140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(1832..3517)
                     /locus_tag="HCV84_RS29145"
                     /old_locus_tag="HCV84_029145"
     CDS             complement(1832..3517)
                     /locus_tag="HCV84_RS29145"
                     /old_locus_tag="HCV84_029145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3920..4339
                     /locus_tag="HCV84_RS29150"
                     /old_locus_tag="HCV84_029150"
     CDS             3920..4339
                     /locus_tag="HCV84_RS29150"
                     /old_locus_tag="HCV84_029150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4645..4908
                     /locus_tag="HCV84_RS29155"
                     /old_locus_tag="HCV84_029155"
     CDS             4645..4908
                     /locus_tag="HCV84_RS29155"
                     /old_locus_tag="HCV84_029155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4898..5197
                     /locus_tag="HCV84_RS29160"
                     /old_locus_tag="HCV84_029160"
     CDS             4898..5197
                     /locus_tag="HCV84_RS29160"
                     /old_locus_tag="HCV84_029160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5259..5636)
                     /locus_tag="HCV84_RS29165"
                     /old_locus_tag="HCV84_029165"
     CDS             complement(5259..5636)
                     /locus_tag="HCV84_RS29165"
                     /old_locus_tag="HCV84_029165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            5705..5884
                     /locus_tag="HCV84_RS29170"
                     /old_locus_tag="HCV84_029170"
     CDS             5705..5884
                     /locus_tag="HCV84_RS29170"
                     /old_locus_tag="HCV84_029170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5912..6442)
                     /locus_tag="HCV84_RS29175"
                     /old_locus_tag="HCV84_029175"
     CDS             complement(5912..6442)
                     /locus_tag="HCV84_RS29175"
                     /old_locus_tag="HCV84_029175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6449..7180)
                     /locus_tag="HCV84_RS29180"
                     /old_locus_tag="HCV84_029180"
     CDS             complement(6449..7180)
                     /locus_tag="HCV84_RS29180"
                     /old_locus_tag="HCV84_029180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(7180..9144)
                     /locus_tag="HCV84_RS29185"
                     /old_locus_tag="HCV84_029185"
     CDS             complement(7180..9144)
                     /locus_tag="HCV84_RS29185"
                     /old_locus_tag="HCV84_029185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8517
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          650..1228
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6453..7180)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag
       61 gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga
      121 gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgccaggtg cgtccagcac
      181 cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt
      241 ggccggcgtc cagccggacg gagccagaaa gagggggaga gaccgtgcgt cggtggcgtc
      301 cagccagcgt aagcagggga tatccccctg aggtcagcca cgccctcgcg gggatgacct
      361 gtatttctcc gtaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa
      421 aaaccgatat gattcaccta tcctggtaat ttattatcag ggaatataaa gacttaatca
      481 ggggacaata tgccacatca gcgcgtccgg tgtccgtctt gtaggtgccg gggaaattgc
      541 gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga ctgcggcggg cgctgcggac
      601 acatgcaaag ttacccacag attccgtgaa taagcagggg actaacatgt gaggcaaaac
      661 ggcaaggcca cgcaggtggc gtttttccat aggctccgcc ctcctgactg agttcacata
      721 aacagacgct ttttcggtgc atctgtggga gccggtggct caaccatgaa tcagtcagga
      781 gtggcgaaac ccgacaggac ttaaagatca tcaccgtttc cgatgaatcg ctccctcttg
      841 cgctctcctg ttccaaccct gccgtttccc ggatacctgt tccgcctttc tcccttgcgg
      901 gaagtgtggc gctttctcat agctcacact ctggtatctc ggctcggtgt aggtcgttcg
      961 ctccaagctg ggctgtaagc aagaaccccc cgttcagccc gactgctgcg ccttatccgg
     1021 taactgttca cttgagtcca acccggaaaa gcacgcaaaa cgccactggc agcagccatt
     1081 ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg
     1141 cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt
     1201 taccacggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg
     1261 gttttttcgt ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct
     1321 ttgatctttt ctactgaacc gcgatccccg tcagttcaga ggaggacgat ggtgccaccg
     1381 ttccaccctg aacatcccgg atatagtttg cctgacatgc gaccagaaga aagcctgaaa
     1441 aaatcaatat aaaaagaaaa acaaccttta cctttttcat ataccttccc tgtatttaaa
     1501 aaccccggta ttcatccctg aacaccggaa gtctgtattt caaatcatcc ttgataactt
     1561 ttgcttacca gttaccatcc cggtaaacaa aggatacaaa gtaatcaaat ttactaatat
     1621 cgattacatt tttaaagtgt ggctgtaata tcgaaaccca tgatttttca acatcaaacc
     1681 aaccattatt aatattatcc tttaaaggca tccccagact ttcaatgaca gaaccatcat
     1741 caccgaagtc ttttgagtat tcaccacctt taaactcttc ggttttctta tcaaaccaat
     1801 gaatatgtaa tttaagcccc atatttacct cttaaagata ttttttaatg tttcgttttg
     1861 gatccggccc tttaacctgc ttacccgttt ttggatcgaa tgccccgagg tgttcgccat
     1921 cactggcgcg gtacccttca agctcaccgt gctgggaatc ccattcataa attttacgct
     1981 ttttatcacc ataccatcgg gcccgcttac caccaccacc ttgtttaggg gttttaggtt
     2041 taccctcttt caactctccc aaccccttaa tatcttcagt cttgggatca ggaaaataat
     2101 catggccgta atctttagtt cccttacgag gttttttctt ttcctcattt aatttatttt
     2161 ctgcatcctt cgcttttttc tccttttgtt tccggctctc catcgccccg gaaagcgcag
     2221 ccttatccct ggataaggat tcattcattt cgtttaaaga tgtctgggca gcttttaatt
     2281 tctcattctg attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc
     2341 catattcatg aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta
     2401 cctttttatc ggcttcctga acagcagccc ttgcaccggg gatcgccttc tctgtcgatg
     2461 caatgcgact gtttaaggtc gcaatatttt tatcttcagc atccagctca gctttcgctt
     2521 tatcatactc tcttttcagc ccctcttccg ggtgagcagc gtcccatgcc tgctggcgac
     2581 gcttttcttc ctccagacgc tgttttacct gtgccggagt aagaacatcc gtcaccgaca
     2641 catagaccgg tttctgtccg ctctctttcg ggaaacgaat aacggcctca cgggagttac
     2701 caccggccgt aaaaccagcc ggacgtgaat cacctttttc agcaataatg ccttttggcg
     2761 gggctttcgc ggccggaaca cctttaggta cgctcacctg cagagacggg agacccggaa
     2821 tcgacacact gaataccccc ggacgttttg tcggtttcgc atccaccaca ggaacactca
     2881 ttggccggcc ggtgacaacc gcaatatgct gccgctcgtc cttcacaata tccgccacac
     2941 gtttagtgac gctgaccgtc gcctggtcca gcggcaggga gctgaccggc gtctccgtta
     3001 ccgtgtctgc cggcaatgac gtcacgattt ttgacatcat tttcggatca tcttttgcta
     3061 tctcagaagg cagcactccg taaatcgcaa tcccccacag accaaactta aacggacctt
     3121 tcagggcagc cagcacatca gcaacagcgg ccgacaacgc atcgccggaa acggataaag
     3181 ccagtccttc agcccccgga gtggccaggg caggcagacc gaaggccatc gcggtggccg
     3241 aagactgtcc gccaccagag ctcccgctat gattgccgct gttaccgcca ttaccaccgg
     3301 gtttactccc gccgttacca aactcccccg ggttatagtt atgaatatcc ccgttaggtg
     3361 tatgcgtcgt accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc
     3421 cacctttacc tgatgtaccg ttcacattcc cactggcctg accaccattg tgtcccagac
     3481 ctgaattacc cggacctcgt ccgtctccgc cactcatata cttttcctct ttgactttta
     3541 aaacaataaa ctaaaataaa atactgtaca tataaccact ggttttatat acagtacaaa
     3601 aattacgctg ccttgttttt catgtcaagt ctgtaaattt gcgtttttgt gaaaatgatc
     3661 aaaaaaacag ccactcacac catgcgcaat cccctccaga tgcaacaaaa accagcacaa
     3721 acggcgccgt tctccggcct cagtatgttt tactgaaatc attatatgag ataccagaaa
     3781 gccccctccg ggacaggaaa agaaacgggg ggtctggttc gggttgttcg gtcactgaac
     3841 acggtcagaa actgatgata agatagcctg cggatatata actcaatgag aaaaaagaat
     3901 taaaggtaag gagacgctca tgagacgttc cggcagcaaa ctgtgtctta tcgccatgat
     3961 aacagcgaca gcaactctca ctgcctgtgc tccaaagggc agcgtggaac agcatacccg
     4021 gcattacgta tatgcttcag atgacggttt tgatcccaat ttttccaccc agaaagctga
     4081 cacaacacga atgatggtgc cttttttccg gcagttctgg gatatgggcg caaaggataa
     4141 agcgaccgga aaatcacgga gtgatgtgca gcaacgcatt cagcagtttc acagtcagga
     4201 atttttaaac tcactccaag gcacaacaca atttgcgggt accgattacc gcagcaaaga
     4261 cctcacaccg aaaaaatcaa ggctactggc tgacaccatt tcagctgttt atctcgatgg
     4321 ctatgaaggc agacagtaag tggatttacc ataacgcgtc attacgcgca ccgctcagac
     4381 gggctcaggg cgtttccggc ggctacggga taaaacaggc aacaaccccc ccggaaaact
     4441 gccggaaacg cgcccgataa attttatcct aatgcatgcc tatgcacgca actgcatggc
     4501 atatgttttg acgtaaaatg gatgccgtaa gccctttttt accgactttc tgcttcatca
     4561 gcctgactga cacgaccata ttctttcaga ccgccccctg ctaaacttcc ttcatattgc
     4621 cgcattaacc cgggaggctc cgcaatgagc aacactacac actacgaaaa cgccaacttt
     4681 cttcgggaac tggctgaaag cctgccccgc atactgccgg aaggtggccc ggacaaagct
     4741 gcgctgttgc agcgactggc taacgaagaa ctggcccagg cagagtatga agatcaggtt
     4801 cgcgccaaag tgaccgccgc ccgtgctgac acgcgtccgg gtatgaccac ggaacaactc
     4861 cgccagcgcc tgcatggccg ttatcaggaa ctgcgcgatg ccgtatgacg tggaatggaa
     4921 gcagggagcc atcgaggatg taacgacgct gtttgactat atcgcggaaa attcatccct
     4981 gtgggatgcg cagaacgtta ccgaccgggt tctcgctgcg gcggacaaac tggccgactt
     5041 cccgcgactc tatgaagtgg actcacgcta tggcgaggac gttcgccgga tcagtctgat
     5101 gggacagcat gtcctctatg atgttgatga ccagaccaga aaagtccggg ttctggccgt
     5161 ggtcggccag cgccagaatc cccatattgt tctctaataa ccggtgacgg ggacgcaatt
     5221 cattgcgtgg ccgtcgccgg ttggacaagc acagcgcgtc agcgttttcc ccacggccac
     5281 caccggcgcg gtgttccggc cggggtctct gctgtctccg gtttgtgttc cagcagtctc
     5341 atggactgca tttcctgccg tagcccggcc acctcctccc tgagtgcttt tatctccgcg
     5401 atcaacgcgt cggaggccat tttttcagtc tcacccgaca gtctcaactc gtcccgctcg
     5461 tgagtctcat cctgtcttaa ctcaccgtac acccggataa gctcgctggt atccaccacc
     5521 cggccgccat cagcttccgt acggtatgat acgcggcctt tcgccatatc ccgatacagg
     5581 gaacttcggg ctttacccgt cagttccgtc gcctgtctca ccgtcaccca cgccattgtc
     5641 tcaccccgct atttttgaga cagtatacct gacaggcgca caccatgccc ggaaggaaag
     5701 aatcatgaca aaacaggaaa aaacagccct caatatggct aaatttatta aggagcagtc
     5761 gctgctattg ctcgataaac tgaatgagct ggatatggat accccggccg acatgtgcga
     5821 gcggctacac gaggatgcgg aaacactctg gcaggtgatg cgggataagc tcggggagga
     5881 ataacagcac cggcatggcc ggtgctggca tttatttttt gccgaacggc caccaccggc
     5941 gggcaggttt gtcctccggc agttcaggta ccggcacgtt aaaatagcgg ctgttatatt
     6001 cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc cgctcactca
     6061 ccacgtcaat catcgcgtta atctgctccg gtgagcactt cccggcatca accacctgca
     6121 caccgttctc gatggcgaac cgctccacct tgcgatagag gtaatcacac agcttcattc
     6181 ctgtgggttt gttccgttcc cattgcggat ttttaccagt gaaaaagtac gtggcttttt
     6241 cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc agatgctggg
     6301 cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc accatatctt
     6361 ccagatgctc gctggtcggc atgatgcagc gctcgccgtt ccggtcctcc gcgtcttcaa
     6421 taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta aagcacgcaa
     6481 acaaccgttc acgatccagc tctgccgttt tgcgggcctc gccggtctgt gggttcacga
     6541 tttcatccac cgacaggatt gcccgctgca ccctgacctg cgtcgcccgg tccgggcaga
     6601 tgtaccagat ttcatcgatg ccgttagctt tgcggttgaa cagatacccg gacaccactt
     6661 cctgataccg gcgacggctc ttgacggtgc gctccacctc aaacgccacc gttttaccgt
     6721 ccagctccgc cagggcgtcc ggcactttca tgcctttaag actcagacgg tgcagaatgc
     6781 gccagccggt ggcccctctg gcttccatat caaggcgcag ggattgcacg gccagccggt
     6841 gcggcagatg tgctgccgac acccttgacg gctgaaacgg aataacatcc gccagcggtt
     6901 cgtcctcgtc gaacgagaac gccacgccat gcggcgtcag cccccacacg ctgacctgac
     6961 ggccgtccac cggatatttg gtccggacca gcagcccttt cgccaccagg ctgtcgagga
     7021 agcggtagac cggctgcacc gccgcaatcc ccagccgccg cgcaaccagc tccgccgtgg
     7081 aacacagctc ttcccgcaac cagcgcaggg tcagccgggt tttctccgcg ttccgcgcct
     7141 gtcgtttcgc gtggctgacg ttgtacagct ccagcgccat cagtaaatcc ccgcaaaatc
     7201 atcatccgct ttcggcccgc cgccgtcggc cgggggccgg aactccgcca ggggatcggt
     7261 tttcgccagc accgggaaac gctgcaacgg gatttcccgg cgctcaaccc gcagcggact
     7321 gctgaacgcc atccgggcca cacccagccc ggtcatgacc gcgaacccgt ccggcaggtg
     7381 ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc tgtccccgct
     7441 gatatgctcc acgttccccg cctcccggct ggcacggcgg cgctccacgt ccaccctgat
     7501 ttccccggtc tgccccgccg cccactgcgc ggactccagg ctggccgccc ggtaaaacca
     7561 gcggatcggc gtgttatcca gtaccgtggt tttcacaaat tcagcgggca gatcctgccc
     7621 gcactgcccg aaatcgccca gcgactggtg cgcgagcaac agattgcagt cccggctgcg
     7681 cagcgtcccc agtgcgttca gtacatactt cgacagcaga tatttaattt catcgagcat
     7741 cacgctgacg tgtggccact cccggaactc gtcccgcgca atgatgattt gcgcgcagcg
     7801 ggcaaacagc attttttgca cacggatcac cgcctcgtcg tccatcgagc cgataacgta
     7861 caggcagccg ccgccgttga taatccccgc cacatcgatg ccactgtccg tctgcaggac
     7921 gctcaggtca gcgacgtttt ccagcctagt gatcaacccc ttcacgtcct ttttcagggc
     7981 ctccggcagc gcgtacgccg ccgccagcac ctgctgaatg tttgccccct gcggaaactg
     8041 ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc
     8101 ggtctcgccc aggttaagcc cggcaatcag caggtttttc agcgcgtact ggtcgagatc
     8161 gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg tgaagtccgg
     8221 acagtgcgcc cggaagacgt gcggcgccca tgcgtcccct ttggggtcga agtacaccac
     8281 cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatca cgctcttgcc
     8341 gctcccgggc aggccgagga tctgaatgtt ggtcttgcgc caggtatcac gcggaaccag
     8401 caccggcttg ccctgctcat ccttacccag aaacaccgcc tgctcctgcc gggcctgctc
     8461 cgaggaaaaa tcggccagcg cattaaccgg taccggctcc ccgaaatgca ggtggcgcac
     8521 gtcggtcatc tctcctgtcc gctggctgct gcgccggctc tcctgattca gtttcgcgtt
     8581 caccgcctcg tcagcagccg caaaatgacc gtcaagcagc aggaacgcca gcagccgcgc
     8641 caacacaatc ccgacagcca gcccgcccgc gcaccaggca atcccgaaca ggttagccgg
     8701 ctgcagcagg taataatctg cgtttgcgta gaggtccgga cgcaggacgg tttctcccca
     8761 gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg ccgcgccggt
     8821 cagccagtac gcgtagttcc agacatgtcg acgtgccctg accatccgcc cgggcgaatc
     8881 cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta tcgcgcttat agcgatcggc
     8941 atactcccgt cgctccttat gactcagccc cacaaaacgc atccgtccgg cccaaagttc
     9001 agccagaccg aaaaacaggc cgataactag gaaagccccg gccagaccgt aatactcctg
     9061 gtacagccag ctgacccacg gataaatctg ccgggccagc tccggaatgt gccacttcca
     9121 ggcaagcgaa tccgtattaa acatgttctc tctccttcag acgatggttt gccggcgtga
     9181 tgtgcagccc ccgctgcaca agccgcggac atgccccaag gcatggcggc ggcggtcacg
     9241 tccgtggtga ttgcgttcag ctttcaccac ggaggccagc ctctttccgc ccca
//
