LOCUS       NZ_CP061121             2208 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium strain S520
            plasmid pS520_2, complete sequence.
ACCESSION   NZ_CP061121
VERSION     NZ_CP061121.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15962526
            Assembly: GCF_015732575.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Plasmid dynamics of mcr-1-positive Salmonella spp. in a general
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2020) Sir Run Run Shaw Hospital, Zhejiang
            University, Qingchundong 3 RD, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method                   :: other: Unicycler v. 0.4.8
            Assembly Name                     :: S520
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015732575.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 01:56:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,237
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,984
            CDSs (with protein)               :: 4,984
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 36 of 130
            Pseudo Genes (incomplete)         :: 105 of 130
            Pseudo Genes (internal stop)      :: 18 of 130
            Pseudo Genes (multiple problems)  :: 27 of 130
            Pseudo Genes (short protein)      :: 1 of 130
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061121.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /strain="S520"
                     /serovar="Typhimurium"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="pS520_2"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.2 N 120.2 E"
                     /collection_date="2017-09"
                     /collected_by="Xiaoting Hua"
     gene            join(1880..2208,1..688)
                     /gene="mobV"
                     /locus_tag="IBY99_RS26030"
                     /old_locus_tag="IBY99_25985"
     CDS             join(1880..2208,1..688)
                     /gene="mobV"
                     /locus_tag="IBY99_RS26030"
                     /old_locus_tag="IBY99_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_094316239.1"
                     /translation="MRSPDWPDGRRNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(750..1184)
                     /locus_tag="IBY99_RS26035"
                     /old_locus_tag="IBY99_25990"
     CDS             complement(750..1184)
                     /locus_tag="IBY99_RS26035"
                     /old_locus_tag="IBY99_25990"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          3..685
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="99.0"
                     /evalue="1.18e-132"
     Typing          1881..2206
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.33"
                     /coverage="99.0"
                     /evalue="1.13e-64"
ORIGIN
        1 gtttttcgcc cgctctgagc aatggctgga gaagaaatac gggaaggatc gtgttgtggc
       61 ggcggtggtt caccgggacg aggccacccc gcacctgtct gcgttcgtgg tgccattgac
      121 gcaggatgga cggctgtccg cgaaagagtt tattggcggg cggtcaaaga tgcgtgaaga
      181 tcagagcacc tacgctgaat cagtaaaaaa actgggtctt gagcgcggca tcgagggaag
      241 ccgcgccacg catcagacag ttcagcacta ctacgaatcg ataaaccgtg gcacccgtag
      301 ccaggtatcg atctcgccgg aggcgctgga gccgcgtgta ttgcggaagg gcattttcac
      361 caaagacgtg gaagatcagg cagccatagc caaacgtcta agccacgcag tgaacgatgg
      421 ctttgcagga accatcgcta tggcctctca gagcgcccag aacgcaaaaa gagcgcgtga
      481 gctacagaag actatggatg cgcagcagaa acgcctacag agcgttacag agccttttaa
      541 ggggctttcc agagagcaga tgaccgaaat cctgatgatg gctcagcggt ttaagcagca
      601 aaaccaggaa aaggaaaaac agcagcgtat tgaacgtgaa aaacagcgac agacgcgatc
      661 gcgtggcatg ggtggtatgg agcgttagtt ttgatgcgat gcaggatagg gctaacgccg
      721 caaagtgacg tcatttgcgg cattccagat taggttctgc cgccctgtat aacgctgagg
      781 ccgtttttta gtgctttctt gtggttgctg acagtacctt tccagccaaa ctgagggttc
      841 agacggtaac tgtaactgcg ccctactttt gggccttgaa gaatcacgga taggtcaatt
      901 agcgccttaa ccgaacggct tacattggtt ttctgcatac ctaaagcgtc ggcaatatcg
      961 gcttgggcaa tttggatgta attttcataa tcgagatcag ctaacagcat catcaagact
     1021 ttgaattgct cccctgtgag ggtcttagca aggatcttta aagcgtcttg gttcatagtg
     1081 aaatgcctct caaaagctga cttctgtttg ggtcggataa ctgcgacaaa actacctacc
     1141 tcttcccctg tagcagtgtc tatctgagta attcggttac tcatttaaga ggctctccca
     1201 aaaaaatagt tatcaataat gataacaacg ttatcaataa tgataactat cgtcaagcga
     1261 gagccgcgcc ccacaagggc tgagagaagg ctctcttctg tatcttatag cgagcgtagc
     1321 gagtgccata cctaaagtac gattagtact tgagatgagt acgattagta cttgaggtga
     1381 gtacgattag tacttgaggt gagtacgatt cttctctcta gcccttggtg tgcgcgggtt
     1441 cccgtatcta tatctatata ttaagggggg aaataaaggc tgaaaaacgc catttttaaa
     1501 catcgaaaaa cgagcttttt tgagtctgga gcgtacggat ttctatgtcc ggggagtgga
     1561 aattccgctt cctcgatcgg acagactcgc ggcgttgccg ctccctaatc cgatctgcgg
     1621 cgagcggttc ctgctctttc aaaagcgata tccagatcct gatttcgtca agggcgagcg
     1681 gagcgagtgc atttcaccct tgacgacgca caaataaaac aggcttggca gtggtgggag
     1741 gtgacgcgga gcgacgcctt ccacctgcca gaccgccgtt gattttgacc ttctggcagc
     1801 ttgctgcccg aacgattttg cgaagcaaag tctagtgagt acactatcat cctgatagtg
     1861 gcccgccgga ggcatcgcaa tgcgatcccc cgactggccg gatggacgcc ggaacgaggg
     1921 ggtaagcatg gcgaattacg cgataatgcg ctgcaaaaag ctgacgggaa tgggcagtgt
     1981 ggccagcgcg ttgcagcact gctacaggga gcgtgagacg ccgaatgctg acgctgaacg
     2041 gacaccagaa aactactgct ctgtgtctca gtcggcagat gaggcgatgg gaaagctgcg
     2101 ggagctgttg ccggaaaaac gccgtaaaga tgcggtactg gctgttgaat acgttatgac
     2161 ggcaagccct gagtggtgga acgaggccac accgcggcag caggcgga
//
