LOCUS       NZ_CP061112             2101 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain 1EC187 plasmid pEC187_4, complete sequence.
ACCESSION   NZ_CP061112
VERSION     NZ_CP061112.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15945289
            Assembly: GCF_016432805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Prah,I. and Saito,R.
  TITLE     Whole genome sequene analysis of OXA-181 producing E. coli from
            Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Prah,I. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JUL-2019
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 485x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 17:06:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,804
            Genes (coding)                    :: 4,570
            CDSs (with protein)               :: 4,570
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 66 of 234
            Pseudo Genes (incomplete)         :: 154 of 234
            Pseudo Genes (internal stop)      :: 43 of 234
            Pseudo Genes (multiple problems)  :: 30 of 234
            Pseudo Genes (short protein)      :: 4 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061112.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="1EC187"
                     /serotype="O8:H9"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC187_4"
                     /geo_loc_name="Ghana: Western region"
                     /collection_date="2018-02-16"
                     /genotype="ST410"
     gene            597..1562
                     /locus_tag="IBZ91_RS24355"
                     /old_locus_tag="IBZ91_24350"
     CDS             597..1562
                     /locus_tag="IBZ91_RS24355"
                     /old_locus_tag="IBZ91_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1566..1832
                     /locus_tag="IBZ91_RS24360"
                     /old_locus_tag="IBZ91_24355"
     CDS             1566..1832
                     /locus_tag="IBZ91_RS24360"
                     /old_locus_tag="IBZ91_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          991..1222
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gattaaaatc cgcatccgtt caccgttttt taaagtccgt taaaagcatg atgccatctc
       61 cgagagttaa tctcgtcaaa tgctaaatcg tgggggtccc ctttggggtt ccgatttagt
      121 gattgacgac accaccgatt aaaaaactta tgcggggtgg atggtttcac gaagtgaggc
      181 catccacctg taagacaggg ttttgttttt attccctgtt ttggtgatcg ggtgtgtgga
      241 aaaggttggg gtaagccgtt cgggggtgct tgttttgggg ggttaaaatt gtggttattt
      301 tttgcgcaat tctcgcgcgt gatccttgta tttatactta agggataaat ggcggatatg
      361 aaatagtggt ttagcccagt aatgacgagg ctttgagtgg gttttgacag gtcaaagaaa
      421 atggagcaga attgaggcgt ttttaatcgg cgttggggag tgcgtcaaca ctccccaaca
      481 tttcgaatgt gtcacctcag cggcaaactc tggtgacatg tactggctcg caatgcacag
      541 gtacgtgatg aatataccac atcaaatcac agcctgccca gatcggagca ggcttaatgt
      601 cagaagataa attcctttcg gactacagcc cccgtgatgc agtttgggat acccagcgca
      661 cgcttaccga ttctgtcggg ggtatctacc agactgctgc tgaattcgag cgctatgcac
      721 tccgtatggc ctcctgtagc ggtttgttac gttttggttg gtctaccatc atggaaaccg
      781 gagaaacgcg cctacggctt cgtagtgcgc aattttgccg tgtccgtcat tgccctgtct
      841 gccagtggag aagaaccctc atgtggcaag cccgttttta tcaggctcta ccgaaaatcg
      901 ttgtggatta cccgtcttcc cgatggttgt ttctgacgtt aactgtcagg aactgcgaga
      961 taggtgaact tggaacagtc cttacagcaa tgaatgcggc gtttaagcga atggaaaagc
     1021 gaaaggagct atcacctgtt caggggtgga tcagggctac ggaggtgacg cgaggtaagg
     1081 atggcagcgc acatccgcat tttcactgtc tgctgatggt gcaaccttct tggtttaaag
     1141 ggaagaacta cgttaagcac gaacgttggg tagaactctg gcgcgattgc ttgcgggtga
     1201 actatgagcc gaatatcgat attcgggcag taaaaactaa gacaggtgag gttgtggcca
     1261 acgttgccga gcaactgcaa agcgcggttg ctgaaacgct gaaatactcc gttaaaccgg
     1321 aagatatggc aaacgatcct gagtggtttc ttgagctgac gcggcagctt cacaagcgcc
     1381 gttttatctc gaccggtggg gcgctaaaaa acgtcctcca gttggatcga gaaaccaatg
     1441 aggatcttgt cattgccgac gatgtagggg atggcactga tgacgggaag cggacggcgt
     1501 ttgtctggga ttcaggtaaa cggcgttaca aacgcgcccc tgagaaggat aaatcggatt
     1561 aacgtatgaa tattaatatt gaatacctga atggaaataa gactattggt ttattttttt
     1621 taagaagtga agcggtgatt cctgacaggt ttaaaaacct tattttgctt attgatggat
     1681 taagttttgg cacatttggt tttcatccgc acgaaggttt tgaggatgaa ttaattttat
     1741 atattcagaa aacaaacgag agggtaaaaa ctctttttgt gaaaatagat ttaaacggta
     1801 tcaagtttgg atttttaaga acgcattctt agttctggaa aagagccagc ggcaggctga
     1861 ggtgataggt acgagattgc atgcaatctc tagtgctctg tctatcctgc attatcctca
     1921 gcattatcct cagcattatc ctcagccttg ccaactcgac accaatgcag gatagacaat
     1981 ccgatgtcaa atgttaacac tctgcgagtg gtacattttc cccggattat cgtcctgagc
     2041 ctgccgctgg ctctctttct accgcctcgc tttgctcgtt gctcaacgcc tcacagacac
     2101 g
//
