LOCUS       NZ_CP061063             6103 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain HKP0018 plasmid pHKP0018.1, complete
            sequence.
ACCESSION   NZ_CP061063
VERSION     NZ_CP061063.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15946735
            Assembly: GCF_014495785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Xanthopoulou,K., Carattoli,A., Wille,J., Biehl,L., Rohde,H.,
            Farowski,F., Krut,O., Villa,L., Feudi,C., Seifert,H. and
            Higgins,P.G.
  TITLE     Antibiotic resistance and mobile genetic elements in extensively
            drug-resistant Klebsiella pneumoniae sequence type 147 recovered
            from Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Xanthopoulou,K., Carattoli,A., Wille,J., Biehl,L., Rohde,H.,
            Farowski,F., Krut,O., Villa,L., Feudi,C., Seifert,H. and
            Higgins,P.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) Institute of Medical Microbiology,
            Immunology and Hygiene, University of Cologne, Goldenfelsstrasse
            19-21, Cologne 50935, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:23:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,596
            CDSs (total)                      :: 5,471
            Genes (coding)                    :: 5,351
            CDSs (with protein)               :: 5,351
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 49 of 120
            Pseudo Genes (incomplete)         :: 66 of 120
            Pseudo Genes (internal stop)      :: 21 of 120
            Pseudo Genes (multiple problems)  :: 13 of 120
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061063.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HKP0018"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHKP0018.1"
                     /geo_loc_name="Germany"
                     /lat_lon="53.4608 N 9.9836 E"
                     /collection_date="2015-02-16"
                     /collected_by="university hospital in northern Germany"
     gene            1..948
                     /locus_tag="IBX29_RS26415"
     CDS             1..948
                     /locus_tag="IBX29_RS26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            1356..1748
                     /locus_tag="IBX29_RS26420"
     CDS             1356..1748
                     /locus_tag="IBX29_RS26420"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            1730..2068
                     /locus_tag="IBX29_RS26425"
     CDS             1730..2068
                     /locus_tag="IBX29_RS26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2049..3503
                     /locus_tag="IBX29_RS28055"
     CDS             2049..3503
                     /locus_tag="IBX29_RS28055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3785..4066)
                     /locus_tag="IBX29_RS26445"
     CDS             complement(3785..4066)
                     /locus_tag="IBX29_RS26445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            4372..5169
                     /locus_tag="IBX29_RS26450"
     CDS             4372..5169
                     /locus_tag="IBX29_RS26450"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="WP_015060052.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
     gene            complement(5576..>5728)
                     /locus_tag="IBX29_RS26455"
                     /pseudo=""
     CDS             complement(5576..>5728)
                     /locus_tag="IBX29_RS26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             4373..5166
                     /name="blaOXA-181"
                     /gene="blaOXA-181"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          14..935
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2050..3500
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagaaagt gcgcacagct tgagctgttt cgggagcgcc tgccccggaa accctactat
       61 tccgacgagc ttacaacggg cctacggatt gcggatgttg cccgcgccct tggtgcgcgt
      121 tacatccagc cgaacgggcc aacgcaccgc cactggatcg tttttgacgt agaccatgca
      181 gccgctacgt tatcttggga tgacgtaggc gctccagccc ctaacattac cgtcacgaac
      241 aaggccaacg gccacgcgca tctgatctac ggactggata cgccgatccg cactgccccg
      301 gatggaaatg cagcccctct gcggtatgcc gccgccatcg aggccgcgct ccgggagaaa
      361 ttaggggcgg atatgggcta ctccgggctg atctgcaaga accccctgca tgagcactgg
      421 ctggtgcagg tatgggaacc ccggctctac gatttagcct ggctatccga ttacctcgat
      481 ctgagctcct acaatggccg caaatcgctt cctgagtacg ggcttgggcg aaactgtacc
      541 ctgttcgaga aaacgcgcct gtgggcctat aaagcgatcc ggcagggctg gccggattac
      601 cccgaatggt tggccgcttg tgttgaccgg gcatcaggat ataacgccca gttcgagcag
      661 ccgctccccg caaacgaagt ccggcacacg gccaagtcca ttgccaaatg gacgcaccag
      721 cacctaagcc ccgcagggtt cagagaggag caggcccgga gaggtgcgaa gggcggcaag
      781 gtatccaagg gagggggaag gccgagcaat agcggtaagg acaagtccga tctattgccg
      841 gaggttctgc ggcttaaggc tcaggggtac accaaccgcg atatagcgga cgatcttcaa
      901 atctccccca gtacggtgag cgtttaccta aagcgtgatc gtccgtgatc gaaacaagcc
      961 ttatcagata atagccccct ttaggggggt tcctgcgctc ttgtctttta gccttaaaaa
     1021 atccttttag cgcggatctt tggcgaggtg gacaactgcg cctgtccgtc catcgccccc
     1081 ctgttttttc ggggggtgtg ggcggcagca gttatccacg gagccgctct ttcccttgat
     1141 cttgctctag ggcttgcagt atcccgccca tggttgaata atgtactaag caaggctaca
     1201 cagaggcccc tctacctgct tcgcggtctc gggcctctgt ggccttgttc aggggtgcta
     1261 cgcccccccg aaaccccctg tggcccccgc tcgatccgcc ccttcgggac ggtctgcgcg
     1321 gtggcttctt ggctctggag aatggacgag gccctatgtc aacaacacag atagagaaag
     1381 attcgagaag gaaggcagtc aatggcgtta tttctagttt ccaactagag ggggaggcca
     1441 taaccgatga attcaaaatt ctacttgaag attatatcaa tggaaaaatt tccaccgatg
     1501 aggtgttagc tatggtaagt gacgaaccca caacaacgga atacgactac gaaatcaccc
     1561 cccgcactct cggtcttggc gggggctgga atctgcgttt gctggagaac ggcgaagagg
     1621 tcggcggggg tgttttcccc ttgccggagc actgcgattt tcgggatgaa aaggcgctcc
     1681 agacgctact cgatagcctc tatgaggacg ccttagcgga ggcctctgca tggctggcca
     1741 gccgttaaag cgcctccgct ctatcaagat ccgggtctca gatgcggagc ttgagcgcct
     1801 gcgggagctc tgccccaagg cccaactggc ggagtggatg cgggagcaat gtctcggtgt
     1861 tgcgcaaccc cagcgccgaa cgccggcgcc taccgtagat ccggcgctct tgcggcagtt
     1921 ggccggaatg ggcaacaacc tgaaccagat cgcccggcgc gttaattccg gggagtgggg
     1981 gccgttggat cggctgcgca tcatagcgga gctgtccgct ataggccgcg agctggagga
     2041 actgcaccat gatcatcaaa ttccataacc gtggggtagg ccggggctcc gggcctgttg
     2101 gctacttgtt gggccgtgac gggaaccggg aaggcgcaac gctcgaccgg ggcaaccctg
     2161 cggtgattga agccctgatt gattccagtc cctacgccaa aaaatacacc tctggcgttc
     2221 tgtcgtttgc cgaggctgat ttacccaggg cgaccaagga caagctgatg agcgactttg
     2281 aaaaggcgct cctgcccgga ctggagggca accagtacgc tgttttgtgg gtagagcacc
     2341 gcgacaaggg gcggctggag ctgaattttg tagtcccgaa catcgaattg cagagcggga
     2401 agcggctgca accctacttc cacaaggccg acaatccgcg cctcgatgcg tggcgaaccg
     2461 tggtaaacgg ggagctgaaa ttgcacgacc cggacgatcc gttaaaccgt caggccgtta
     2521 tcacgccaaa ggatttgccg agggggcaca aggaggccgc tcaggccatt acagacgggc
     2581 ttttacgcat ggccggagca ggggagatcc agagccgcga tgatgtgcgc aaagccttgc
     2641 agggtgcggg tttcagcgtg gtacgcgaaa ccaaaaacag cctgagcatt gccgacccgg
     2701 acggggggcg gaacatccga ttaaaggggg tgctgtatga gcaagatttt agacatggcg
     2761 aaggacttcg agcagagctc gaagcagcag gccaacgcta tcgagaagcg tctgaccgaa
     2821 gaattcacga ggcacgagaa agctatctcc ggggcgttga aatcaagcga ggagaaaatg
     2881 agcgccgcta ttgccgagca cagccagaaa atgggctggc tgttgctcaa aacgtggatc
     2941 tggatgctgg tcagcatcgt cctgttcttg agcgcaacga gcgggatcct ctggtatcag
     3001 ggggaattga tagcggagcg gcaagccact ctccaagcct tggcagagaa gggcggcaag
     3061 ctaaagctga cccagtgcgg gaagaaattg gagcaccttt gcgtgcagat ctccgacccc
     3121 aatccaaccg tttgggggaa ggatacctat tggatcgtgc cgaaggggta ctaaatgacg
     3181 ggattggagc gggaattgct ctgcgcgttc gaggctttgc agagcgcata cgagcagcag
     3241 cacaaggcat ggcaggaaag ctacgcgaac ttggcgagca tgtacgagag caccaaacaa
     3301 gaggccgaga tcagcagggc agcagcagag cgattgacga ggcaggtgaa cggcttaacg
     3361 cagcaagtgg acggcttgag caagcagctg gagggcttga ccgggctagt cagcaagttg
     3421 caaaagaaag ggaacacagg ctcctgaagg cactggatcg aggctgggag cgtggagaca
     3481 gatcgcgagg ctttgagcgt tgacggaaca aaacggccgt cagttataaa aaaaggaaag
     3541 tgctatcact ttccttttat cggcgggaat tatcgttgag ttcttctatg ccatttttca
     3601 tagtagtaac tgtaatcttc atctctacca ccagattcaa tccagtcatc atactcaggg
     3661 ctatttttga aatctgaatc agacatgcta actccttgtt attagttggc ttctatggaa
     3721 gctagcgcac cgtgaagtta tgatcaacga tgtagtccac atcattgtcg aaatgtgcgt
     3781 tcagctagtc ttcggtgttt tctgctttat ttctagcggc gattctcgcg tctgcccatg
     3841 cggcagactt tttagcctgt tctcgttgtt cttcggttga agactcgctg ttgagaactt
     3901 tgttgctcgt ttgtttgacc accgaatcca aacctgttgt tgctgcaaaa tgagctaggt
     3961 taacaactcc cttacctaac aacttggcac tagcgattgc ggtttcttta gccccaaaag
     4021 catcagacac ctcctcaagc gctttagata ggttaaaaaa tcccatactc cccgataact
     4081 tgcagagttt attaagtatc attgcagcaa agatgaaatc aatgatttat caaaaatgat
     4141 tgaaaggtgg ttgtaaataa tgttacaatg tgtgagaagc agtctaaatt cttcgtgaaa
     4201 tagtgatttt tgaagctaat aaaaaacaca cgtggaattt aggtaatagt taaagcttgt
     4261 gcctgattgt tgccacaatt ggtcagcagt ttgctaggga atgagagggc agagaatgat
     4321 aagcttagca cttaatttgt atggatatat tgcattaagc aaggggacgt tatgcgtgta
     4381 ttagccttat cggctgtgtt tttggtggca tcgattatcg gaatgccagc ggtagcaaag
     4441 gaatggcaag aaaacaaaag ttggaatgct cactttactg aacataaatc acagggcgta
     4501 gttgtgctct ggaatgagaa taagcagcaa ggatttacca ataatcttaa acgggcgaac
     4561 caagcatttt tacccgcatc tacctttaaa attcccaata gcttgatcgc cctcgatttg
     4621 ggcgtggtta aggatgaaca ccaagtcttt aagtgggatg gacagacgcg tgatatcgcc
     4681 gcttggaatc gtgaccatga cttaattacc gcgatgaagt actcagttgt gcctgtttat
     4741 caagaatttg cccgccaaat tggtgaggca cgtatgagta aaatgctgca cgccttcgat
     4801 tatggcaatg aggatatctc gggcaatgta gacagttttt ggctcgatgg tggtattcgc
     4861 atttcggcta cccagcaaat cgctttttta cgcaagctgt atcacaacaa gctgcacgtt
     4921 tctgagcgta gtcagcgcat cgtgaaacaa gccatgctga ccgaagccaa tggcgactat
     4981 attattcggg ctaaaacggg atactcgact agaatcgaac ctaagattgg ctggtgggtt
     5041 ggttgggttg aacttgatga taatgtgtgg ttttttgcga tgaatatgga tatgcccaca
     5101 tcggatggtt tagggctgcg ccaagccatc acaaaagaag tgctcaaaca ggagaaaatt
     5161 attccctaga agtggttagc gcgtatttgt gtgaaatagc cgtcatataa gctgtaaaga
     5221 tatatggaca aaatgctgat gctcgatgcg cttgtagtta atggctcata cttaatcggt
     5281 caggcctaat cgacttaagc tccgcgcgtt aactttcagc ctgagcatta gcatcacatt
     5341 cctaaacgta aaaagcctag ccaatttgct aggctttttt gttatctcaa tggccatggt
     5401 cactcaacat gggggtgtga ataaaggagt ggcgatgtca gtaaaggtga atgccgctgc
     5461 gataaagtgc ctagcccatc aaggattgta ggctttcttg gctgctaaag cgtttgacga
     5521 aaaagtccaa gaaggcgctg atattggttc tccatcattt tcatctaccg gtttactata
     5581 ggtcaaccag gccgtccttg ccggcgcttg ggacgcagac gatggcatca aatgcctcgc
     5641 tcaaattcgt ttctacagag gcgctttggg accgcattcg ctttgctttc atgggggcat
     5701 ctgtcaatgt cagacatgaa tcctccgata taggttgatt tttgatctct tcaacacttg
     5761 gtgttgtccg actagagaga cttatatgga tcagtattct tgcactgaca atggtgccac
     5821 tcaatcccaa ggatcgacct agttagaggc gtagatcaag caattcaaaa cagcgcagaa
     5881 gcagaaggag gtggaaatat gttccagggt gaaaggttac atgcaactaa cgttgctctc
     5941 taggatgtgc ctgctgtaac gtattgtcca gaaaagacaa acccccgtta tcgcttgcat
     6001 cttggcggag ctgcgaaaaa cgggggccgt gacgaatcac aagagaagtc tatcacatgg
     6061 atcatgccaa acaaccccac gccgccaata agcgcggtgg tgc
//
