LOCUS       NZ_CP060993             3372 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC31 plasmid pEC31-7, complete sequence.
ACCESSION   NZ_CP060993
VERSION     NZ_CP060993.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904669
            Assembly: GCF_022558625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558625.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:05:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,238
            CDSs (total)                      :: 5,118
            Genes (coding)                    :: 4,841
            CDSs (with protein)               :: 4,841
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 90 of 277
            Pseudo Genes (incomplete)         :: 190 of 277
            Pseudo Genes (internal stop)      :: 59 of 277
            Pseudo Genes (multiple problems)  :: 56 of 277
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060993.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC31"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC31-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-12-06"
                     /collected_by="Long Sun"
     gene            complement(join(3260..3372,1..406))
                     /locus_tag="H9212_RS25995"
     CDS             complement(join(3260..3372,1..406))
                     /locus_tag="H9212_RS25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_052969336.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTALSVTGGIFLGCWGL
                     MEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKGENTWTVGD
                     KNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<294..1136)
                     /locus_tag="H9212_RS26000"
                     /old_locus_tag="H9212_25740"
                     /pseudo=""
     CDS             complement(<294..1136)
                     /locus_tag="H9212_RS26000"
                     /old_locus_tag="H9212_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1126..1449)
                     /locus_tag="H9212_RS26005"
                     /old_locus_tag="H9212_25745"
     CDS             complement(1126..1449)
                     /locus_tag="H9212_RS26005"
                     /old_locus_tag="H9212_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1513..1704
                     /locus_tag="H9212_RS26010"
                     /old_locus_tag="H9212_25750"
     CDS             1513..1704
                     /locus_tag="H9212_RS26010"
                     /old_locus_tag="H9212_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3020..3253)
                     /locus_tag="H9212_RS26015"
                     /old_locus_tag="H9212_25755"
     CDS             complement(3020..3253)
                     /locus_tag="H9212_RS26015"
                     /old_locus_tag="H9212_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1786..1866)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(4..1136)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.561"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2938..3371)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.333"
                     /coverage="99.0"
                     /evalue="2.34e-53"
ORIGIN
        1 ccccccaggt ttttgcctcg agtttcgcca gcgtgttttc ctgctctctg atagtcagga
       61 tatttttcgc aatcttgctt tcctgccact ccatcagccc ccagcacccc aggaaaatgc
      121 cgcccgtgac gctgagcgcg gtcagcgccg gaaacagtat ccgccctttg atcacccgcc
      181 agagcagttt tttctgctgc cgggacagtt cggcctctgt ggtgctgaac tgcatgctca
      241 cggtggtgct cagcgtatcc agttgctttt tcacggatgt tgtatgttcg ctgatagcgt
      301 ctctgatttt ctgcctgttt aagctcaatg ccttgtctac agactcttcg agcttcctga
      361 actcgctgtt cagcatgttc tcggtagaag cggctcgctc tttcagtttc ttctcgaagt
      421 ctgttcccat ttgtaaaaga ttgctcatac agcgccccct ttagcctgag attccgcccc
      481 ccctccgggt cggcaatact gatgctgctt ttggttgtcc tcaccacctc aaaacctgcc
      541 gctgtaagcg cctctgtgac atcctgacgc gtttttattg ctccggcatg gtaaagagcc
      601 tcaagacccc gtgtaatcgc ttctgcggcc tcctgtttcg cttttggcag attatttggc
      661 gtgacaagcg tccgtctgtt ctccggtgcg ttcgggtcac gcagcccgta atgatgatta
      721 accagcgtct gccaggcatt tatcctcggc ctgtccgcgc ggtcgtagta cggctggagg
      781 cgcttaccgc tctgtagctc catgttcggg atgacaaaat tcagttcgag ccgcccctta
      841 tcctggtgtt ccacccagag aatgctgtac tggtcttttt cgagaccggg cataagaaca
      901 cgctcaaagc tcgtcatcac tctttcgcgt tcacccggcg gtaactcctt ctccgcgaac
      961 gacagaacgc ctgaagtgta tttcttcgag aatggcgtgg aatcaatgag ttcccgcact
     1021 tcttccgggt taccccggag caccgttgcg ccttcgcggt tacgctcccg ccccagcagg
     1081 taatcgaccg ggccacttcc accgcctttc cccctggcat gaaatttaac tatcatcccg
     1141 taccccctgt tccctgacag cctgccgcaa ctggcgcaat tcaccctcga tggccatcag
     1201 tgcggccacc acatgaaccc ggtcaatcga agaccactgg ccgctgttca ccttccgggc
     1261 tgtctggttc aggttgttcc cgatggcagc cagatgccgc agcaatggcg gcgatagtgt
     1321 cggcagtttt cctgtgcgtg caaccggctc ccccagacag acccgccgca tccattccgc
     1381 cagtcgttta ccctcgcagc gttccagtaa ccgggcgtgc tcctcatcgg taacccgtat
     1441 cgtgagcatc ttctcgcgtt tcatcggtat cattccccct aacaaatccc ccatacaagg
     1501 agccttaaga ccatgaccaa acaggaaaaa acagccctta acatggctcg ctttatcaga
     1561 agccagacgc taaccctgct ggaaaaactc aacgatctgg acgcggacga acaggcagac
     1621 atctgcgaat cacttcacga ccacgccgac gaactctacc gcagttgcct cgcacgcttc
     1681 ggcgatgacg gtgaaagcaa ctgaaacatg cagctcccgg agacgttcac agcttgcctg
     1741 tgagcggatg ccgggagcag acaagcccgc cagggcgcgt cagtgcgggt gtcggggcgc
     1801 agccctgacc cagttcacag agcgatagcg atgtgtatac tggcttatct cattttgcat
     1861 cggtgcagac atggatggga agtgcaccat gtcaggagta aaaagccgca cagatgtaaa
     1921 aacagcgcac ccgataaaca gcacacacgc ggacactggc acaggcgcgt aagtgcacaa
     1981 tgcttgcatc tgtgactctt ccgcttcctc gcccactcgc tcactacgtt cggtcgctgg
     2041 ggctgcggcg agcggtggga tcactctcgt tgtgatgcag atccgttttt ttttttgcac
     2101 tggagttatc aggagttgcg gtgcacatgg gatcgtgcga tatgccggat ggtgaaaggt
     2161 tggagggaac ggcaacaact cacccctccc agtgaaggga cgggtagttt gccgctctcg
     2221 atatgccgga cggagccaag gccatcgatc acggggcgcg tcagtggaag aacccgcgcc
     2281 tgcgtgacgt atgcgtcttc gccgcaccat ctcacccgtc acgccaccac ataccgggaa
     2341 ctgccgccaa ggtctaactc aaccaatcta gccgcgctac atacggtttt tgtggcaact
     2401 catcaggtgt ccgctgtcga tgaaactctc tgcgccggta gcggaactct cacgcgtgcg
     2461 gtcgccgtcg tcacagctcc cccaaccgca aacgattctc gcaaatacgc aagtatctgc
     2521 aaaaacaggt ttttttcggg gttcctggga gactgtgctg ttgaaaaatt cttgaagaat
     2581 acagaaggct catgcaaact gaatgaactg gtttctgttg tggatctcaa cagtttccac
     2641 aggaacattg aggaggtcag agacctcaat gaacccgaaa cagctcgcct tcctggcgag
     2701 ctgttttcgt tctacgcccg ccgacgaaag atcgcaagtg caataacgag atttttcacg
     2761 tctttacgct caaaaagtaa aaaaaccatg ttttcccctg gctgaatccc caaaaacgcg
     2821 tgaaatgcgt tttcaggatg ttgcatagct atgcacaaat acggcacacg atttgtttcg
     2881 ctcgccccag ccgtcgaact ggtcaaaatg gcaagatttc gtcacttccc cggtcacatc
     2941 tccattgatg gcccactccg aacccgtgcc gcgcgttcct gtacctcatg tctgtaccgg
     3001 gcacgctcct gttcatccct tagctgttgt tcgcgataaa taaccgactc aagcgatcca
     3061 cttgcccggc taatctctgc acctgctgac tcaatatcac gacgcgctcg ttcagtatcg
     3121 cgttgttccg ttgcgtaacc tcgtgcatct tctgcaattc cgcgaaggcg ctctcccact
     3181 cgttcagccg ccgcatatag tcctgttgca aatgctctaa ggcgttcagc aaatgccctt
     3241 ccagttccgt catactcgtt tactccctga ccagcctcac ggcgttcttg tcgcccaccg
     3301 tccaggtgtt ttcccctttc accccgtccg gcaacaccag aaatttcccg ttctcgccat
     3361 tgacgaacgt ca
//
