LOCUS       NZ_CP060985             3373 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC3 plasmid pEC3-7, complete sequence.
ACCESSION   NZ_CP060985
VERSION     NZ_CP060985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904648
            Assembly: GCF_022559325.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559325.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:19:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,006
            CDSs (total)                      :: 4,884
            Genes (coding)                    :: 4,640
            CDSs (with protein)               :: 4,640
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 77 of 244
            Pseudo Genes (incomplete)         :: 172 of 244
            Pseudo Genes (internal stop)      :: 49 of 244
            Pseudo Genes (multiple problems)  :: 48 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060985.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC3-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-01-22"
                     /collected_by="Long Sun"
     gene            join(3316..3373,1..134)
                     /locus_tag="H9191_RS24870"
                     /old_locus_tag="H9191_24650"
     CDS             join(3316..3373,1..134)
                     /locus_tag="H9191_RS24870"
                     /old_locus_tag="H9191_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1451..1684)
                     /locus_tag="H9191_RS24875"
                     /old_locus_tag="H9191_24655"
     CDS             complement(1451..1684)
                     /locus_tag="H9191_RS24875"
                     /old_locus_tag="H9191_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1691..2251)
                     /locus_tag="H9191_RS24880"
                     /old_locus_tag="H9191_24660"
     CDS             complement(1691..2251)
                     /locus_tag="H9191_RS24880"
                     /old_locus_tag="H9191_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2283..2939)
                     /locus_tag="H9191_RS24885"
                     /old_locus_tag="H9191_24665"
                     /pseudo=""
     CDS             complement(<2283..2939)
                     /locus_tag="H9191_RS24885"
                     /old_locus_tag="H9191_24665"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2929..3252)
                     /locus_tag="H9191_RS24890"
                     /old_locus_tag="H9191_24670"
     CDS             complement(2929..3252)
                     /locus_tag="H9191_RS24890"
                     /old_locus_tag="H9191_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(216..296)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.366"
                     /coverage="93.0"
                     /evalue="1.88e-14"
     Typing          complement(1369..2939)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.817"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaccctgct ggaaaaactc aacgatctgg acgcggacga acaggcagac atctgcgaat
       61 cacttcacga ccacgccgac gaactctacc gcagttgcct cgcacgcttc ggcgatgacg
      121 gtgaaagcaa ctgaaacatg cagctcccgg agacgttcac agcttgcctg tgagcggatg
      181 ccgggagcag acaagcccgc cagggcgcgt cagtgcgggt gtcggggcgc agccctgacc
      241 cagttcacag agcgatagcg atttgtatac tggctttcct cattttgcat cggtgcagag
      301 tggatgggaa gtgcaccatg tcaggagtaa aaagccgcac agatgcgtaa aaacagcgcg
      361 cccgataaac aacacacacg cggacactgg cacaggcgcg taagtgcaca atgcttgcct
      421 ctgtgactct tccgcttcct cgcccactcg ctcactacgt tcggtcgctg gggctgcggc
      481 gagcggcggg atcactctcg ttgtgatgca gatccgtttt tttttttgca ctggagttat
      541 caggagtagc ggtgcacatg ggatcgtgcg atatgctgga tggtggaagg tcggagggaa
      601 cggcaacaac tcacccctcc cagtgaaggg acgggtagtt tgccgctctc gatatgccgg
      661 acggagccaa ggccatcgat cacggggcgc gtcagtggaa gaacccgcgc ctgcgtgacg
      721 tatgcgtctt cgccgcacca tctcacccgt cacgccacca cataccggga actgccgcca
      781 aggtctaact caaccaatct agccgcgcta catacggttt ttgtggcaac tcatcaggtg
      841 tccgctgtcg atgaaactct ctgcgccggt agcggaactc tcacgcgtgc ggtcgccgtc
      901 gtcacagctc ccccaaccgc aaacgattct cgcaaatacg caagtatctg caaaaacagg
      961 tttttttcgg ggttcctggg agactgtgct gttgaaaaat tcttgaagaa tacagaaggt
     1021 tcatgcaaac tgaatgaact ggtttctgtt gtggatctca acagtttcca caggaacatt
     1081 gaggaggtca gagacctcaa tgaacccgaa acagctcgcc ttcctggcga gctgttttcg
     1141 ttctactccc gccgacgaaa gatcgcaagt gcaataacga gatttttcac gtctttacgc
     1201 tcaaaaagta aaaaaaccat gttttcccct ggctgaatcc ccaaaaacgc gtgaaatgcg
     1261 ttttcaggat gttgcatagc tatgcacaaa tacggcacac gatttgtttc gctcgcccca
     1321 gccgtcgaac gggtcaaaat ggcaagattt cgtcacttcc ccggtcacat ctccattgat
     1381 ggcccactcc gaacccgtgc cgcgcgttcc tgtacctcat gtctgtaccg ggcacgctcc
     1441 tgttcatccc ttagctgttg ttcgcgataa ataaccgact caagcgatcc acttgcccgg
     1501 ctaatctctg cacctgctga ctcaatatca cgacgcgctc gttcagtatc gcgttgttcc
     1561 gttgcgtaac ctcgtgcatc ttctgcaatt ccgcgaaggc gctctcccac tcgttcagcc
     1621 gccgcatata gtcctgttgc aaatgctcta aggcgttcag caaatgccct tccagttccg
     1681 tcatactcgt ttactccctg accagcctca cggcgttctt gtcgcccacc gtccaggtgt
     1741 tttccccttt caccccgtcc ggcaacacca gaaatttccc gttctcgcca ttgacgaacg
     1801 tcacccccca ggtttttgcc tcgagtttcg ccagcgtgtt ttcctgctct ctgatagtca
     1861 ggatattttt cgcaatcttg ctttcctgcc actccatcag cccccagcac cccaggaaaa
     1921 tcccgcccgt gacgctgagc gcggtcagcg ccggaaacag tatccgccct ttgatcaccc
     1981 gccagagcag ttttttctgc tgccgggaca gttcggcctc tgtggtgctg aactgcatgc
     2041 tcacggtggt gctcagcgta tccagttgct ttttcacgga tgttgtatgt tcgctgatag
     2101 cgtctctgat tttctgcctg tttaagctca gtgccttgtc tacagactct tcgagcttcc
     2161 tgaactcgct gttcagcatg ttctcggtag aagcggctcg ctctttcagt ttcttctcga
     2221 agtctgttcc catttgtaaa agattgctca tacagcgccc cctttagcct gagattccgc
     2281 cccccctccg ggtcggcaat actgatgctg cttttggttg tcctcaccac ctcaaaacct
     2341 gccgctgtaa gcgcctctgt gacatcctga cgcgttttta ttgctccggc atggtaaaga
     2401 gcctcaagac cccgtgtaat cgcttctgcg gcctcctgtt tcgcttttgg cagattattt
     2461 ggcgtgacaa gcgtccgtct gttctccggt gcgttcgggt cacgcagccc gtaatgatga
     2521 ttaaccagcg tctgccaggc atttatcctc ggcctgtccg cacggtcgta gtacggctgg
     2581 aggcgcttac cgctctgtag ctccatgttc gggatgacaa aattcagttc gagccgcccc
     2641 ttatcctggt gttccaccca gagaatgctg tactggtctt tttcgagacc gggcataaga
     2701 acacgctcaa agctcgtcat cactctttcg cgttcacccg gtggtagctc cttctccgcg
     2761 aacgacagaa cgcctgacgt gtatttcttc gaaaatggcg tggaatcaat gagttcccgc
     2821 acttcttccg ggttaccccg gagcaccgtt gcgccttcgc ggttacgctc ccgccccagc
     2881 aggtaatcga ccgggccact tccaccgcct ttccccctgg catgaaattt aactatcatc
     2941 ccgtaccccc tgttccctga cagcctgccg caactggcgc aattcaccct cgatggccat
     3001 cagtgcggcc accacatgaa cccggtcaat cgaagaccac tggccgctgt tcaccttccg
     3061 ggctgtctgg ttcaggttgt tcccgatggc agccagatgc cgcagcaatg gcggtgccag
     3121 tgtcggcagt tttcctgtgc gtgcaaccgg ctcccccaga cagacccgcc gcatccattc
     3181 cgccagtcgt ttaccctcgc agcgttccag taaccgggcg tgctcctcat cggtaacccg
     3241 tatcgtgagc atcttctcgc gtttcatcgg tatcattccc cctaacaaat cccccataca
     3301 aggagcctta agaccatgac caaacaggaa aaaacagccc ttaacatggc tcgctttatc
     3361 agaagccaga cgc
//
