LOCUS       NZ_CP060984             8776 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC3 plasmid pEC3-6, complete sequence.
ACCESSION   NZ_CP060984
VERSION     NZ_CP060984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904648
            Assembly: GCF_022559325.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559325.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:19:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,006
            CDSs (total)                      :: 4,884
            Genes (coding)                    :: 4,640
            CDSs (with protein)               :: 4,640
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 77 of 244
            Pseudo Genes (incomplete)         :: 172 of 244
            Pseudo Genes (internal stop)      :: 49 of 244
            Pseudo Genes (multiple problems)  :: 48 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060984.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC3-6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-01-22"
                     /collected_by="Long Sun"
     gene            complement(278..601)
                     /gene="mobA"
                     /locus_tag="H9191_RS24810"
                     /old_locus_tag="H9191_24590"
     CDS             complement(278..601)
                     /gene="mobA"
                     /locus_tag="H9191_RS24810"
                     /old_locus_tag="H9191_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            873..1067
                     /locus_tag="H9191_RS24815"
                     /old_locus_tag="H9191_24595"
     CDS             873..1067
                     /locus_tag="H9191_RS24815"
                     /old_locus_tag="H9191_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_022631127.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1554..1715
                     /locus_tag="H9191_RS25125"
     CDS             1554..1715
                     /locus_tag="H9191_RS25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071784682.1"
                     /translation="MHEPPVQPDHCALSGNYRLESNPVRHAKAPLAAAIGNRVSRARFV
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            1916..2593
                     /locus_tag="H9191_RS24820"
                     /old_locus_tag="H9191_24600"
     CDS             1916..2593
                     /locus_tag="H9191_RS24820"
                     /old_locus_tag="H9191_24600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_022631128.1"
                     /translation="MENKAVTISTQLAIFIDSTIARPDLLFNSLNSEIGEVIDAMPQTL
                     PLPADAPADIPRVIGTSSFGKFNLNISLNRIDFVENYTPTEEIEKSVTEFKLKCNTLIA
                     TIFQKYGVVRIGLVGNYYIPHKNPSQLIADLYLNENKRNLDEITIRINNRDTFKNIQIN
                     NVINLSQGQVTAQNYNGNAVIVQLDYNSTDNNTPLEEGLTLALFKEKSIAYNSKHVEDV
                     SGL"
                     /name="hypothetical protein"
     gene            2590..2973
                     /locus_tag="H9191_RS24825"
                     /old_locus_tag="H9191_24605"
     CDS             2590..2973
                     /locus_tag="H9191_RS24825"
                     /old_locus_tag="H9191_24605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_022631129.1"
                     /translation="MKFFKPKTDAIEYIRVEPSFSKTSSNINNAKSGSDTNSQPGFILR
                     SQIEEMIKNHRPKTEKEIKAESEGLIRLCSMAFIFLIVLSIFMLIVYFGIKDRELPDFA
                     NNLMALMTTLVGAIGGFIYGTKK"
                     /name="hypothetical protein"
     gene            complement(3375..3608)
                     /locus_tag="H9191_RS24830"
                     /old_locus_tag="H9191_24610"
     CDS             complement(3375..3608)
                     /locus_tag="H9191_RS24830"
                     /old_locus_tag="H9191_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3615..4133)
                     /locus_tag="H9191_RS24835"
                     /old_locus_tag="H9191_24615"
     CDS             complement(3615..4133)
                     /locus_tag="H9191_RS24835"
                     /old_locus_tag="H9191_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_072665564.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTTSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4171..4869)
                     /locus_tag="H9191_RS25130"
                     /old_locus_tag="H9191_24620"
                     /pseudo=""
     CDS             complement(<4171..4869)
                     /locus_tag="H9191_RS25130"
                     /old_locus_tag="H9191_24620"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4811..5134)
                     /locus_tag="H9191_RS24845"
                     /old_locus_tag="H9191_24625"
     CDS             complement(4811..5134)
                     /locus_tag="H9191_RS24845"
                     /old_locus_tag="H9191_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5198..5389
                     /locus_tag="H9191_RS24850"
                     /old_locus_tag="H9191_24630"
     CDS             5198..5389
                     /locus_tag="H9191_RS24850"
                     /old_locus_tag="H9191_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_100783622.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6059..6217
                     /locus_tag="H9191_RS25135"
     CDS             6059..6217
                     /locus_tag="H9191_RS25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072672540.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(6454..6542)
                     /locus_tag="H9191_RS24855"
                     /old_locus_tag="H9191_24635"
     tRNA            complement(6454..6542)
                     /locus_tag="H9191_RS24855"
                     /old_locus_tag="H9191_24635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6506..6508),aa:Lys,seq:ctt)
                     /name="tRNA-Lys"
     gene            6719..7609
                     /locus_tag="H9191_RS24860"
                     /old_locus_tag="H9191_24640"
     CDS             6719..7609
                     /locus_tag="H9191_RS24860"
                     /old_locus_tag="H9191_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241468327.1"
                     /translation="MPDLEIMPLQSPDFYKKNKRAIYEGYKCNCTKDWKKEDRFVVYKA
                     DCTGIDEIINTEISDDNIDTVIKLAEKYTSDKIIISGGHTVVNLNDRFSVSNEVEKSAK
                     FCIDYIIKSTHELNIKPDFLMEINDFYMEKSNGEDIDGGNIYRKLATSPYIIPEVINNY
                     IIEKQNQHNIKINCFYVSEKNMADRFKRHIKRKEKEKPFFKENNSVFMNVDGSSFEVIK
                     NNKPTCAAGNAATFRSIRYKISSNKIFDNYTSHIGVFPLCSMANVINGYKAPASFYSNF
                     NLPCLLIFFGTSCFK"
                     /name="hypothetical protein"
     gene            7621..8754
                     /locus_tag="H9191_RS24865"
                     /old_locus_tag="H9191_24645"
     CDS             7621..8754
                     /locus_tag="H9191_RS24865"
                     /old_locus_tag="H9191_24645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123863911.1"
                     /translation="MKINSIQWPVTFFATSFGAGIFFLPQTVGPKVIGTHGTITILILS
                     SLASLLGQFTFYRFILITDKKEYVSSAILFIGKKAANAISIAFLLSMLLIAIINITTMI
                     NIICFSLSLNGSERIFVSLIISATLGSSFIFFNSKIEHLASGIAFPSLLIVLFLSIYFY
                     SHPAHPHSTEIQNISTSNLLTLFPVVLFSFNFSPCIQRFAQNKKDTAGDLRFFMFGILL
                     ILIFISFIVFSLSNIFSPSDIKNMREINSDALSYSANVTKNSFVAYISILTMILITSGA
                     YTGTLTGVVDTIVSLGFKNKTLIVFVVSVITFIAGEANFEIIKVIAALSTPIIAITVFI
                     IPSAFFLKTNCNSIFRKIMMTLNLLIGIGICFLAIIL"
                     /name="hypothetical protein"
     Typing          complement(611..836)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="99.0"
                     /evalue="4.88e-75"
     Typing          complement(5475..5560)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          1097..1626
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.227"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5573..6131
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.929"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(282..601)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.39e-62"
     Typing          complement(3272..4821)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacgagttta tcttaatcgt cacaaccaaa tgtggaaaat ggggggatca tccaaaccat
       61 ttaacataac aacaatatta cgcgcaccgc caaaacgcgc tgaacgcgat tctgacgcag
      121 acaccgcaaa aagagatgtt ttccccggga taaaccggaa aaatgcgtca ggatcgcttt
      181 cagctcactg catagctatg catgaaagtg aatggcgatc ggtttggggg cttacggcgt
      241 tcataccgtc tgttttcgac agtttctctc cgggaagcta atctgccata agcctggata
      301 acagggcacg gtgatactcc gtaatagcga tcagcacctc cgcatactcc ctgtccccga
      361 cacgcttgcc gtcgataaag agttttttct gcaacgcccc cagtcgcata acttctttca
      421 gcacccggtc atcagtcagt gagttaactt tcttaccgag agctgccgcc cgtaaaaaac
      481 cagaaacggt cttgccgcat tcagcagccc tttttctgat ttcctggtct tcttctgccg
      541 tcagacgaac agggcgactg atagcccgcc tgcgcgtatt acttccactt cgttttgtca
      601 taaaacatgg ctccgtatct gacatggggt gtcggggcga agccctgacc aaggtaattg
      661 taatagcgtg catgtatgcg cggtataaca attacacatc ctgtcctgtc tgcaagttcg
      721 aattaccaac aaagcactgc tgttttttgg cagtgacagt gttgtctgac tgaaatgtcc
      781 gtcggcagaa taccggcggt aagacggtcg aaaagtggtg cgcgatataa tcacacgacg
      841 cagatgacag acagaacgga gaaagcagaa agatgaacaa gcagcagcaa accgcactca
      901 acatggcaaa attcataaaa agccagagcc tgacgctgct cgaaaaactg gacgcactcg
      961 atgctgacga gcaggccacc atgtgtgaga agctgcacga actcgcagaa gaactccaga
     1021 acagcataca gacacgcttt gaagtagaaa atagcacaga gatataacgc tcagggcagg
     1081 atgccgaacg gcacaacaga gcgtaaggag aaaatgtcac ataaggcgct cttccgcttc
     1141 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtgt cagctcactc
     1201 aaaggcggta atacgattat ccacagaatc aggggataaa gccggaaaga acatgtgagc
     1261 aaaaagcaaa gcaccggaag aagccagcac cgcaggcgtt tttccatagg ctccgccccc
     1321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1501 cacgctgtag gtatctcggc ccggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1561 aaccccccgt tcagcccgac cactgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1621 cggtaagaca tgcaaaagca ccactggcag cagccattgg taaccgagtt agcagagcga
     1681 gatttgtcca gatgcttaca gagttcttga agtgatggcc tgactacggc tacactagaa
     1741 ggacagtatt tggtatctgc gctctgctga agccagttac ccggttagac aatctgccag
     1801 gattagctaa ccttcgaaaa accacctgcc agggtggttt tttcgtttaa gatactcaca
     1861 acacgttaat ttcgcaatac ttcaattaat accaatgcaa aataggaaag aaaaaatgga
     1921 aaacaaggcc gtcacaatat caacacagct agcaatattt attgacagca ccatagctag
     1981 accagatctt ctctttaata gcctaaactc agaaattggc gaagttatag acgcaatgcc
     2041 acagacgctt cccctgcctg cagatgcacc cgcagatatc cctcgcgtta ttggcacatc
     2101 gtcctttggc aagttcaact taaacatatc actaaacaga atagattttg tagaaaacta
     2161 cacccctact gaggagatcg aaaaatcagt aacggaattc aaattaaagt gcaatacgct
     2221 aatagcaaca atattccaaa aatatggagt cgtaagaatc ggcctagttg gtaactatta
     2281 catcccacat aaaaatccat ctcaactaat agctgatctg tatttaaacg aaaacaaaag
     2341 aaatttagat gaaataacaa taagaataaa taaccgcgat acattcaaaa atatacaaat
     2401 aaacaatgtc atcaatttaa gccaaggaca agtaaccgca cagaactaca atggaaatgc
     2461 ggtgatagtt caacttgact acaactcaac cgataataac acaccacttg aagaagggtt
     2521 aaccttagcc ctatttaaag aaaaatccat tgcttataac tccaagcatg ttgaggatgt
     2581 gagtggatta tgaaattttt caaaccaaaa acagatgcga ttgaatacat tcgtgtagaa
     2641 ccatcattct caaaaacaag tagcaatata aataatgcga aatcaggaag tgacacaaac
     2701 tcacagcctg gattcatttt aagatctcaa atagaggaaa tgataaaaaa ccacagaccg
     2761 aaaacagaaa aagaaataaa ggctgaaagc gaaggtctta taaggctttg ctccatggcc
     2821 tttatatttt taatagtttt atcaattttt atgttaatcg tttatttcgg aataaaagat
     2881 agagaactac cagattttgc aaataacctg atggcactaa tgacaacatt agttggagcg
     2941 ataggtggtt ttatatatgg aacaaaaaaa taacacagac aaaaacacaa ataaaaaaaa
     3001 caaataacac acaagcacac cagccccata cttcggggct tttacaaccg ttacctgaca
     3061 ccatttacca taacgtctca ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca
     3121 gcagacaggt aaaaatggca acaaaccacc cgaaaaaatg ccgcgatcgc gcctgataaa
     3181 ttttaaccgt atgaatacct atgcaaccag atggtacagg ccacattccc cccacttaat
     3241 ccactgaagc tgccattttt catggtttca ccatcccagc gaagggccat ccagcgtgcg
     3301 ttcctgtatt tccggctgac gctcccgttc cagggataac acatgttcgc gctcctgtat
     3361 caaccgttcc tctcttatct ccagttctcg ctgtataact ggctcaagcg ttctgtctgc
     3421 tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc gctcgttcag catcgcgttg
     3481 tcccgttgcg taagcgaaaa catcttctgc aattccacga aggcgctctc ccattcgctc
     3541 agccgctgca tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg tatttccagc
     3601 tctgtcactc tgtgtcactc cttcagatgt acccactctt tcccctgaaa gggaatcacc
     3661 tccgctgatt tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg cacaaactcc
     3721 acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct gatttcttcc
     3781 aggttcgtct gtatccgtcc gccaagatac cagagcgtcc cgccactcgt ggtaaacagg
     3841 agaaagacta tccccagtaa catcatgccc gtattccctg ccagctttaa cacgtccttc
     3901 ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt tttccagcga tttctctgtc
     3961 gaggctgtga acagggctat agcgtctctg attttcgtct cgtttgaggt cacagcctcg
     4021 ctgacagact cgccgagctt cctgaactcg tcgttcagca ttttctctgt agattcggct
     4081 ctctctttca gctttttctc gaactccgcg cccgtctgta aaagattgct cataaaatgc
     4141 tcctttcagc ctgatattcc tcccgccgtt cgggtctgca atgctgatac tgcttcgcgt
     4201 caccctgacc acttcaagcc ccgcctctgt gagcgcctga atcacatcct gacggccttt
     4261 tatctctccg gcatggtaaa gtgcgtctat accgcgtgta acgctctcag caagtgcctg
     4321 tttcgtttcc ggcaggttat caggaagggt cagcgtccgg cggttctccg gggcgttggg
     4381 gtcatgcagt ccgtaatggt gattcaccag cgtctgccag gcatcaattc tcggcctgtc
     4441 tgcgcggtcg tagtacggct ggaggcgttt tccggtctgt agctccatgt tcggaatgac
     4501 aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc tgtactgatt
     4561 tttttcgaga ccgggcatca gtacacgctc aaagctcgcc atcacttttt cacgtcctcc
     4621 cggcggcagt tccttctccg cgaacgacag aacaccggac gtgtatttct tcgcaaatgg
     4681 cgtggcatcg atgagttccc ggacttcttc cggattaccc tgaagcaccg ttgcgccttc
     4741 gcgattacgc tccctcccca gcaggtaatc aaccggacca ctgccaccac cttttcccct
     4801 ggcatgaaat ttaactatca tcccgcgccc cctgttccct gacggccaga cgcagccggc
     4861 gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca ccggaagacc
     4921 actgcccgct gttcacctta cgggctgtct gattcaggtt atttccgatg gcggccagct
     4981 gacgcagtaa cggcggtgcc agtgtcggca gcctcccgga acgggcaacc ggctccccca
     5041 ggcagacccg ccgcatccat accgccagtt gtttaccctc acagcgttca agtaaccggg
     5101 catgttcatc atcagtaacc cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta
     5161 ccccatgaac agaaatcccc cttacacgga ggcatcagtg actaaacagg aaaaaaccgc
     5221 ccttaacatg gcgcgcttca tcagaggcca gacattaacg ctgctggaga agctcaacga
     5281 actggacgcg gatgaacagg cagatatctg tgaatcgctt cacgaccacg ccgatgagct
     5341 ttaccgcagc tgccttgcac gcttcgggga tgacggtgaa accctctgac acaagcagct
     5401 tccggagacg ctcacagctt gcctgtgagt ggatgccggg agcagacaag ccctccaggg
     5461 cgcgtcagca ggttttagcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     5521 cggagtgtat actggcttaa ctatgcggca tcagtgcaga ttgtatgaaa agtgcctcat
     5581 gccgggtgtg aaattccgca cagatgcgta aggagaaaat gcgcgtccag gcgcttttcc
     5641 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggtgtcagct
     5701 cactcaaagg cgataatacg attatccaca gaatcagggg ataacgccgg aaagaacatg
     5761 tgagcaaaaa gcaaagcacc ggaacaagcc agcgccgatg ccgtttttcc ataggctccg
     5821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6001 tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6121 caacccggta agacacgcct taacgccact ggcagcagcc attggtaact ggacaaggtg
     6181 gattgagata ttaagagttc ttgaagtggt ggcctaactg aggctacact agaaggacag
     6241 tatttggtat ctgcgctcca ccaagccagt taccttcggg aaaaagagtt ggtagctctt
     6301 gattccggca aacgaaccac cgttggtagc ggtggttttt ttgtttacag gcagcagatt
     6361 acgcgcagaa aaaaaggatc tcaagaggat cctttgatct tttttcaaaa cccctctcaa
     6421 ttcaacaaat ggtgggggaa acgatcaaca aaatggcgga gagggtagga ttcgaaccta
     6481 cgatacgctt gtcgcatatg gcctcaagag agccctgact tagaccactc gcccacctct
     6541 ccacaaaaaa caatacatat ttaacaaata acatccaaga cactataaga gaaaaaatag
     6601 agacgcatca cataaataaa ggagacatca atgatcaaac actaacatga tctctatcat
     6661 tgatatgtat aagtcataat tagcaaaata ttttctttgt gacaaaggaa gcataactat
     6721 gccagaccta gaaataatgc ccttacaaag tccagatttt tataaaaaaa acaaaagagc
     6781 aatatacgag ggctataaat gtaattgtac aaaagactgg aaaaaagaag acagatttgt
     6841 tgtatacaaa gcagattgca caggaatcga tgagataata aacacagaaa tatcagatga
     6901 caacatagat actgtaataa aactagccga aaaatacaca tcagacaaga tcattatatc
     6961 tggtggacat actgttgtga atttgaatga ccgtttttca gttagtaatg aagttgaaaa
     7021 atcggctaaa ttttgcattg actacataat aaaatcaaca catgagctaa atataaaacc
     7081 tgatttcctc atggaaataa atgatttcta catggaaaaa tcaaatggcg aagatatcga
     7141 tggtggtaat atttatagaa aactagccac atcaccctat ataataccag aagtaataaa
     7201 taactatatt atagagaaac agaatcagca taatataaaa ataaattgtt tctatgttag
     7261 tgaaaaaaac atggcagatc gttttaaacg acacattaaa agaaaagaaa aagaaaagcc
     7321 attttttaaa gaaaacaact ctgtttttat gaatgtcgat ggttcatcat ttgaagtgat
     7381 aaaaaataac aaaccgactt gtgcagcagg taacgcagct acatttcgct cgattcgata
     7441 taaaatttca tcgaacaaga tatttgacaa ttacacatca cacataggtg tattcccatt
     7501 gtgctcaatg gctaatgtaa ttaatggcta taaagcacca gcatcttttt atagtaattt
     7561 taatcttcca tgtttactta ttttctttgg aacatcatgc ttcaaatgaa aggttcaaaa
     7621 atgaagataa atagcatcca atggccagta acattctttg caacatcatt tggggcaggg
     7681 atttttttcc taccccagac tgtaggcccc aaagtcatag gaactcacgg aacaataaca
     7741 atactgattt tatcatcttt ggcatcttta ttagggcaat ttacatttta tagattcatt
     7801 ttaattacag acaaaaaaga atacgttagt tctgccatat tatttatcgg aaaaaaagcc
     7861 gcaaatgcta tcagtattgc attcctatta tcaatgctgt taatagcaat tataaacata
     7921 acaacaatga taaatattat ttgctttagc ctaagtctaa atggttcaga gcgaatattt
     7981 gtgtcactga ttatttctgc aacccttggt tcttctttta tcttttttaa ttcaaagata
     8041 gagcatctcg catcaggaat agcatttcca tctttgctta ttgtactgtt cttatctata
     8101 tatttctatt cacatccagc acatccacat tctacagaaa tacaaaatat tagtacaagt
     8161 aatttactta ctctatttcc agttgtttta tttagtttta atttttcacc atgtatacaa
     8221 cgctttgctc aaaacaaaaa agatacagcc ggtgatttac gttttttcat gtttgggata
     8281 ctattaattt taatatttat ctcattcatc gtgttctcat taagcaatat attctcacct
     8341 tcagacataa aaaacatgcg cgagattaac agcgatgcat tatcatatag cgccaacgta
     8401 acaaaaaata gtttcgttgc ttacatttcc atattaacca tgattttaat taccagcggt
     8461 gcatatacag gtacactaac aggcgttgtt gatacaatag tatcccttgg atttaaaaac
     8521 aaaacattaa tcgtttttgt cgtatctgtt atcacattta tagcaggcga agccaatttc
     8581 gaaattataa aagttattgc tgcattatca accccaatta tagcaataac agtattcatt
     8641 atcccatcag cattcttttt aaaaacaaat tgtaacagta tttttagaaa aataatgatg
     8701 acgttaaacc tgctcatagg tattggaata tgcttcctag ccatcatatt atgattattc
     8761 ctcacccaaa tattct
//
