LOCUS       NZ_CP060978             6647 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC4 plasmid pEC4-5, complete sequence.
ACCESSION   NZ_CP060978
VERSION     NZ_CP060978.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904649
            Assembly: GCF_022559305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559305.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:04:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,976
            CDSs (total)                      :: 4,856
            Genes (coding)                    :: 4,607
            CDSs (with protein)               :: 4,607
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 93 of 249
            Pseudo Genes (incomplete)         :: 154 of 249
            Pseudo Genes (internal stop)      :: 45 of 249
            Pseudo Genes (multiple problems)  :: 40 of 249
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060978.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC4"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC4-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-02-26"
                     /collected_by="Long Sun"
     gene            complement(<70..528)
                     /locus_tag="H9192_RS25010"
                     /old_locus_tag="H9192_24435"
                     /pseudo=""
     CDS             complement(<70..528)
                     /locus_tag="H9192_RS25010"
                     /old_locus_tag="H9192_24435"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(470..793)
                     /locus_tag="H9192_RS24725"
                     /old_locus_tag="H9192_24440"
     CDS             complement(470..793)
                     /locus_tag="H9192_RS24725"
                     /old_locus_tag="H9192_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            857..1048
                     /locus_tag="H9192_RS24730"
                     /old_locus_tag="H9192_24445"
     CDS             857..1048
                     /locus_tag="H9192_RS24730"
                     /old_locus_tag="H9192_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1718..1876
                     /locus_tag="H9192_RS25015"
     CDS             1718..1876
                     /locus_tag="H9192_RS25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            2374..2601
                     /locus_tag="H9192_RS24735"
                     /old_locus_tag="H9192_24455"
     CDS             2374..2601
                     /locus_tag="H9192_RS24735"
                     /old_locus_tag="H9192_24455"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(2598..4166)
                     /gene="cea"
                     /locus_tag="H9192_RS24740"
                     /old_locus_tag="H9192_24460"
     CDS             complement(2598..4166)
                     /gene="cea"
                     /locus_tag="H9192_RS24740"
                     /old_locus_tag="H9192_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            4426..4863
                     /locus_tag="H9192_RS24745"
                     /old_locus_tag="H9192_24465"
     CDS             4426..4863
                     /locus_tag="H9192_RS24745"
                     /old_locus_tag="H9192_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4944..5363
                     /locus_tag="H9192_RS24750"
                     /old_locus_tag="H9192_24470"
     CDS             4944..5363
                     /locus_tag="H9192_RS24750"
                     /old_locus_tag="H9192_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5681..5914)
                     /locus_tag="H9192_RS24755"
                     /old_locus_tag="H9192_24475"
     CDS             complement(5681..5914)
                     /locus_tag="H9192_RS24755"
                     /old_locus_tag="H9192_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5921..6439)
                     /locus_tag="H9192_RS24760"
                     /old_locus_tag="H9192_24480"
     CDS             complement(5921..6439)
                     /locus_tag="H9192_RS24760"
                     /old_locus_tag="H9192_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1134..1221)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1232..1790
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.365"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..480)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3e-100"
ORIGIN
        1 agggagtgtc agcgtcctgc ggttctccgg ggcgttcggg tcatgcagcc cgtaatggtg
       61 attcaccagc gtctgccagg catcaattct aggcctgtct gcgcggtcgt agtacggctg
      121 gaggcgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct caagccgtcc
      181 cttgtcctgg tgctccaccc acaggatgct gtactgattt ttttcgagac cgggcatcag
      241 tacacgctca aagctcgcca tcactttttc acgtcctccc ggcggcagct ccttctccgc
      301 gaacgacaga acaccggacg tgtatttctt cgcaaatggc gtggcatcga tgagttcccg
      361 gacttcttcc ggattaccct gaagcaccgt tgcgccttcg cggttacgct ccctccccag
      421 caggtaatca accggaccac tgccaccacc ttttcccctg gcatgaaatt taactatcat
      481 cccgcgcccc ctgttccctg acggccagac gcagccggcg cagctcatcc ccgatggcca
      541 tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg ttcaccttac
      601 gggctgtctg attcaggtta tttccgatgg cggccagctg acgcagtaac ggcggtgcca
      661 gtgtcggcag ttttccggaa cgggcaaccg gctcccccag gcagacccgc cgcatccata
      721 ccgccagttg tttaccctca cagcgttcaa gtaaccgggc atgttcatca tcagtaaccc
      781 gtattgtgag catcctctcg cgtttcatcg gtatcattac cccatgaaca gaaatccccc
      841 ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg cccgctttat
      901 cagaagccag acattaacgc tgctggagaa gctcaacgaa ctggacgcag atgaacaggc
      961 cgatatttgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct gcctcgcacg
     1021 tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1081 tctgtgagcg gatgccggga gctgacaagc ccgtcagggc gcgtcagcag gttttagcgg
     1141 gtgtcggggc gcagccctga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     1201 catgcggcat cagtgcggat tgtatgaaaa gtacgccatg ccgggtgtga aatgccgcac
     1261 agatgcgtaa ggagaaaatg cacgtctagg cgcttttccg cttcctcgct cactgactcg
     1321 ctacgctcgg tcgttcgact gcggcgagcg gtactgactc acacaaaaac ggtaacacag
     1381 ttatccacag aatcagggga taaggccgga aagaacatgt gagcaaaaga ccaggaacag
     1441 aaagaaggcc acgtagcagg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct
     1681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa ggcacgcctt
     1801 aacgccactg gcagcagcca ctggtaaccg gattagcaga gcgatgatgg cacaaacggt
     1861 gctacagagt tcttgaagta gtggcccgac tacggctaca ctagaaggac agtatttggt
     1921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2041 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt
     2101 tagaagagga ggatggtgcg atggtccctc cctgaacatc acgtatatag ttagcctgac
     2161 atccaacaag gaggtttatc gcgaatattc ccacaaaaaa tcttttcctc ataactcgat
     2221 ccttataaaa tgaaaagaat atatggcgag gtttaattta tgagcttaag atactacata
     2281 aaaaatattt tatttggcct gtactgcaca cttatatata tataccttat aacaaaaaac
     2341 agcgaagggt attatttcct tgtgtcagat aagatgctat atgcaatagt gataagcact
     2401 attctatgtc catattcaaa atatgctatt gaacacatag cttttaactt cataaagaaa
     2461 gattttttcg aaagaagaaa aaacctaaat aacgcccccg tagcaaaatt aaacctattt
     2521 atgctatata atctactttg tttggtccta gcaatcccat ttggattgct aggacttttt
     2581 atatcaataa agaataatta aatccctaac acctcattta tagtattaag tttattctta
     2641 tcaatatagg agcatagaat tcctgtaaca atagcaatac cccaaatacc taatgtagtt
     2701 ccagcaagca agctaaaaag taaagcaaca acataactca cccctgcatc tgcagctttc
     2761 ttctctaatg taagaaatag tggcttccag tcacctgtat ccttaatttt taggatatca
     2821 gataccacat catatccaaa agaaacatgc cccgtaatct tcaagtactt ggcaaactga
     2881 tctaaatgtt tagcccagtc atcatacttc accgatgcca acgcattaaa aatagcatca
     2941 cgatcggctt tgctgaattt cttatttaaa acatccttgt atttttcaaa agcagcgaga
     3001 gcttcattca cattgccgat tttcttacct ttagacttat cagcaagttc ctgtgccatt
     3061 ttcgaatatt tttcaccata tttttcagtc agcgtttgat aaaagctaac tgttgcatca
     3121 acagcatcct taatctgtga attaaggaga ttattctgtg cttttttcaa attttcttca
     3181 gcttcatgaa cacgagcgat accggcatta cgattattac tgacctgaga aatagccttc
     3241 tggatctgag ttatatcagc atttatccgg ttaatacgtg tttctgatgc tgttacctgt
     3301 ttttgttttt cttctctaat cttaccggcc ccaacccgtc gtctggttgc ttcaaaaaaa
     3361 ggacggttct gaagcggatc attggctctt ggtgatagtt ttttgaccag ctcatccagt
     3421 tctttatatt tagcggatgc ctgagccagt tcatttcgtt ttccagcgag cgttttcatt
     3481 tctgcatcac gggcatggat actggagctt aaacgagaat tgagagtctt aatctctcca
     3541 tccattttca ccacttcaga ttgtgcagca gaaagttttt tttgggcgat ctcaacagct
     3601 ttagcttctt cactcaatgc agccagtcgt ttctcttcag cttcagccag tttcaactgg
     3661 cgttctgttt cagccttctc ccgttcaatc tctttacgtc gttgttctgc ttcctgaaaa
     3721 gccttttctg ctgcttccgc ttctttacgg gctttttctt ctgctttcgc aaggcgcaaa
     3781 cgctctgctt ccgcctgcat agctgcatta ttagcatgag caagctctgt tgctgaaggc
     3841 gtacgtgagg cattgtgacg aagagcctca ttcacgatat ccttcaggcg ctgagtcagc
     3901 gcatccctgt ttgcctttgc tttcgcctgt gcttccgctg cagcttttgc ccgggcagcc
     3961 tgctctgcct gtgttttctt taattgagca gtagaccatt tagcagttgc atgaatagct
     4021 gcagaacttt cacttttact gcctcctttt ccacctccgc cgccagagcc actcccgtca
     4081 ggagtaccat tcaaaagagt aataattacc tgtcccttat catcataagg aacaccatct
     4141 ttatagtacg ctaccgcggt ttccattata aaatcctctt tgacttttaa aacaataagt
     4201 taaaaataaa tactgtacat ataaccactg gttttatata cagcataaaa gctacgccgc
     4261 tgcattttcc ctgtcaagac tgtggacttc catttttgtg aaaacgatca aaaaaacagt
     4321 ctttcacacc acgcgctatt ctcgcccgat gccacaaaaa ccagcacaaa cattaccgtt
     4381 ctcagacctc attatgtttt actgaaacta tgagatgaga catctatggg acactgtcac
     4441 tttatggcat ggcacacact ccgggacgca ctaaaaatga caggcagatc gcgttcacag
     4501 ttttaccgtg atatgcgcgc aggccttgtc agttaccgta ccggcaggga cggacgacgg
     4561 gagtttgaaa ccagtgaact gatccgggca tacggcgaat taaagcagaa tgagacacca
     4621 gaaaggcaca gtgagggaca tgcagaaaat ccacatgatc agcagacaga acgcattctc
     4681 cgggagctga atgagctgaa acaatgcctg acgctgatgc ttgaggataa acaggcacag
     4741 gatatggatc gcagacgcca ggaagcagaa cgggaacagc tacaaaatga gatagcccag
     4801 ctcaggcagg cactggaact ggaaaagaaa cggggattct ggtccaggtt gttcggtcgc
     4861 tgaacgctgt cagagactga tgataaaata gtcttcggat aataactcac cgagaataaa
     4921 tactttaagg tagggagaca ctcatgagac gtaccggaaa caaactttgt cttatcgcca
     4981 tgataacagc aacagtagct ctcacagcct gtaccccaaa gggcagcgtg gaacaacata
     5041 cccggcatta cgtatatgct tctgatgacg gttttgatcc caacttttcc acccaaaaag
     5101 ccgacacaac acgaatgatg gtgccttttt ttcggcagtt ctgggatatg ggagctaaag
     5161 ataaagcgac aggaaaatca cggagtgatg tgcaacaacg cattcagcag tttcacagcc
     5221 aggaattttt aaactcactc cggggcacaa ctcaatttgc gggtactgat taccgcagca
     5281 aagaccttac cccgaaaaaa tccaggctgc tggctgacac gatttctgcg gtttatctcg
     5341 atggctacga gggcagacag taagtggatt taccataatc ccttaattgt acgcactgct
     5401 aaaacgcgtt cagcgcgatc acggcagcag acaggtaaaa atggcaacaa accacccgaa
     5461 aaactgccgc gatcgcgcct gataaatttt aaccgtatga atacctatgc aaccagaggg
     5521 tacaggccac attaccccca cttaatccac tgaagctgcc atttttcatg gtttcaccat
     5581 cccagcgaag ggccatccag cgtgcgttcc tgtatttccg gctgacgctc ccgttccagg
     5641 gataacacat gttcgcgctc ctgtatcagc cgttcctctc ttatctccag ttctcgctgt
     5701 ataactggct caagcgttct gtctgctcgc tcaagtgctg cacctgctga ctcaactgca
     5761 tgacccgctc gttcagcatc gcgttgtccc gttgcgtaag cgaaaacatc ttctgcaatt
     5821 ccacgaaggc gctctcccat tcgctcagcc gctgcatata gtcctgttgc agctgctcta
     5881 aggcgttcag caaatgtgtt tccagctctg tcactctgtg tcactccttc agatgtaccc
     5941 actctttccc ctgaaaggga atcacctccg ctgatttccc gtacggaagg acaaggaatt
     6001 tcctgttccc gtcctgcaca aactccacgc cccatgtctt cgcgttcagt ttctgcaatg
     6061 tctcttcctg cttcctgatt tcttccaggt tcgcctgtat cctccctcca agataccaga
     6121 gcgtcccgcc actcgcggta aacaggagaa agactatccc cagtaacatc atgcccgtat
     6181 tccctgccag ctttaacacg tccctcctgt gctgcatcat cgcctctttc accccttccc
     6241 ggtgtttttc cagcgattcc tctgtcgagg ctgtgaacag ggctatagcg tctctgattt
     6301 tcgtctcgtt tgatgtcaca gcctcgctta cagattcgcc gagcctcctg aactcgttgt
     6361 tcagcatttt ctctgtagat tcggctctct ctttcagctt tttctcgaac tccgcgcccg
     6421 tctgcaaaag attgctcata aaatgctcct ttcagcctga tattcttccc gccgttcgga
     6481 tctgcaatgc tgatactgct tcgcgtcacc ctgaccactt ccagccccgc ctcagtgagc
     6541 gcctgaatca catcctgacg gccttttatc tctccggcat ggtaaagtgc atctatacct
     6601 cgcgtgacgc cctcagcaag cgcctgtttc gtttcaggca ggttatc
//
