LOCUS       NZ_CP060968            12124 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC7 plasmid pEC7-6, complete sequence.
ACCESSION   NZ_CP060968
VERSION     NZ_CP060968.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904651
            Assembly: GCF_022559265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12124)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559265.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:19:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,758
            CDSs (with protein)               :: 4,758
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 86 of 271
            Pseudo Genes (incomplete)         :: 194 of 271
            Pseudo Genes (internal stop)      :: 50 of 271
            Pseudo Genes (multiple problems)  :: 53 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060968.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC7"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC7-6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-04-26"
                     /collected_by="Long Sun"
     gene            complement(join(<12124..<12124,1..797))
                     /locus_tag="H9194_RS25540"
                     /old_locus_tag="H9194_25340"
                     /pseudo=""
     CDS             complement(join(<12124..<12124,1..797))
                     /locus_tag="H9194_RS25540"
                     /old_locus_tag="H9194_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TraD N-terminal domain-containing protein"
                     /name="TraD N-terminal domain-containing protein"
     gene            complement(1050..1781)
                     /gene="traT"
                     /locus_tag="H9194_RS25545"
                     /old_locus_tag="H9194_25345"
     CDS             complement(1050..1781)
                     /gene="traT"
                     /locus_tag="H9194_RS25545"
                     /old_locus_tag="H9194_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="WP_023565183.1"
                     /translation="MKTKKLMMVTLVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIWL
                     EPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKADKM
                     DLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVN
                     YTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNL
                     KFEEAKPVLEDQLAKSIANIL"
                     /name="traT"
     gene            complement(1813..2310)
                     /locus_tag="H9194_RS25550"
                     /old_locus_tag="H9194_25350"
     CDS             complement(1813..2310)
                     /locus_tag="H9194_RS25550"
                     /old_locus_tag="H9194_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="WP_000605857.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLADWGNAETVFMLFFIVLWQGLFI
                     WLFSQIRKKRDVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRQVSGCISI
                     KKWGQNQLKDQYDGSEKLDFGGIEQTPTIYYNPSTGYPMHGGFDSAGNTFGTRWQDYYD
                     RQ"
                     /name="entry exclusion protein"
     gene            complement(2326..5148)
                     /gene="traG"
                     /locus_tag="H9194_RS25555"
                     /old_locus_tag="H9194_25355"
     CDS             complement(2326..5148)
                     /gene="traG"
                     /locus_tag="H9194_RS25555"
                     /old_locus_tag="H9194_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="WP_001007018.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAVA
                     VMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPLSL
                     TTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDYVQN
                     CVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLKDKLN
                     LDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNVTINAL
                     KEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTGIAIGIF
                     PLLVLAAVFNKLTLSVLKGYVFALMWLQSWPLLYAILNSAMTFYAKQNGAPVVLSEISQ
                     IQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTASAAGSVVDG
                     NYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDASGAMSRLPV
                     GINATRQIAVAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRGSSDSVTSGAD
                     STMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMYGDAHAEWGVKP
                     KILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASYDARHDIDAKASKDFKEASDYFT
                     SRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQ
                     MSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQIQPGVDNAWRE
                     SRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRNDVKHQVDNMVTEYKGN
                     IGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSAQERMPGADSPQELMKR
                     AKEYQDKYKQ"
                     /name="traG"
     gene            complement(5145..6518)
                     /gene="traH"
                     /locus_tag="H9194_RS25560"
                     /old_locus_tag="H9194_25360"
     CDS             complement(5145..6518)
                     /gene="traH"
                     /locus_tag="H9194_RS25560"
                     /old_locus_tag="H9194_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="WP_001517790.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQP
                     GVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQLQR
                     FVKQIMSNAAGYFFDLALQTTVPEIKTAKDFVQKMASDINSMNLSSCQAAQGIIGGLFP
                     RTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNINIMW
                     NALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGGTAKIY
                     HCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGFISSTTI
                     PVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYDEAVIGHI
                     NDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNYHFGGSTR"
                     /name="traH"
     gene            complement(6518..6898)
                     /gene="trbF"
                     /locus_tag="H9194_RS25565"
                     /old_locus_tag="H9194_25365"
     CDS             complement(6518..6898)
                     /gene="trbF"
                     /locus_tag="H9194_RS25565"
                     /old_locus_tag="H9194_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="WP_001389370.1"
                     /translation="MRENKSNPELKIRSTERDYKYISRITGRYAGLSLVFLTAGIVLWT
                     VMDIIFDACIDSWKADPELNNSSYMWNILIYAIPYALYALAAGFLVTFFSVPNVRINIR
                     KYRDIPAEMSYAPGEHIKGGQE"
                     /name="trbF"
     gene            complement(6885..7226)
                     /gene="trbJ"
                     /locus_tag="H9194_RS25570"
                     /old_locus_tag="H9194_25370"
     CDS             complement(6885..7226)
                     /gene="trbJ"
                     /locus_tag="H9194_RS25570"
                     /old_locus_tag="H9194_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="WP_001443031.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIVTGIIVSLN
                     IPFIRQGLFYPASPVEIVVSLSLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESSEI
                     SEGTYNERK"
                     /name="trbJ"
     gene            complement(7156..7701)
                     /gene="trbB"
                     /locus_tag="H9194_RS25575"
                     /old_locus_tag="H9194_25375"
     CDS             complement(7156..7701)
                     /gene="trbB"
                     /locus_tag="H9194_RS25575"
                     /old_locus_tag="H9194_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     thiol-disulfide isomerase TrbB"
                     /protein_id="WP_000059851.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAAG
                     TSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSVFS
                     YTLDGQGDTAFPEALPVPPEVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDAAGF
                     MARVDTVLQMYGGKKGAK"
                     /name="trbB"
     gene            complement(7688..7972)
                     /gene="traQ"
                     /locus_tag="H9194_RS25580"
                     /old_locus_tag="H9194_25380"
     CDS             complement(7688..7972)
                     /gene="traQ"
                     /locus_tag="H9194_RS25580"
                     /old_locus_tag="H9194_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="WP_000624109.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLAE
                     LIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
                     /name="traQ"
     gene            8053..8367
                     /locus_tag="H9194_RS25585"
                     /old_locus_tag="H9194_25385"
     CDS             8053..8367
                     /locus_tag="H9194_RS25585"
                     /old_locus_tag="H9194_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001324103.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLPGNAAIIALIYAASHSLPVNAFPD
                     YLVISLLSIAAGIVVLWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI
                     "
                     /name="hypothetical protein"
     gene            complement(8369..8716)
                     /gene="trbA"
                     /locus_tag="H9194_RS25590"
                     /old_locus_tag="H9194_25390"
     CDS             complement(8369..8716)
                     /gene="trbA"
                     /locus_tag="H9194_RS25590"
                     /old_locus_tag="H9194_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="WP_001287910.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVIN
                     IACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWSGV
                     MWFFIRGLMTG"
                     /name="trbA"
     gene            complement(8732..9475)
                     /gene="traF"
                     /locus_tag="H9194_RS25595"
                     /old_locus_tag="H9194_25395"
     CDS             complement(8732..9475)
                     /gene="traF"
                     /locus_tag="H9194_RS25595"
                     /old_locus_tag="H9194_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TraF"
                     /protein_id="WP_001030393.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVSAAP
                     RQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKAML
                     AHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQLAQV
                     INGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSIRPL
                     SYGFISQDDLAKQFLNVSEDFKPNF"
                     /name="traF"
     gene            complement(9468..9725)
                     /locus_tag="H9194_RS25600"
                     /old_locus_tag="H9194_25400"
     CDS             complement(9468..9725)
                     /locus_tag="H9194_RS25600"
                     /old_locus_tag="H9194_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="WP_000864318.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGMLA
                     AAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
                     /name="conjugal transfer protein TrbE"
     gene            complement(9752..11560)
                     /gene="traN"
                     /locus_tag="H9194_RS25605"
                     /old_locus_tag="H9194_25405"
     CDS             complement(9752..11560)
                     /gene="traN"
                     /locus_tag="H9194_RS25605"
                     /old_locus_tag="H9194_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="WP_000821841.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQGF
                     KPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDIL
                     SPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRTARM
                     ELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQKSVPM
                     TITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMKLRLVV
                     RVKKASYTPYVVWSESCPFSKEQGKLTKTECTEAGGNRTLVKDGQSYSMYQSCWAYRDT
                     YVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGKVMVCGGD
                     VFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGGDVRAFTGQAKFCKKAAA
                     GYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLEKKRSYC
                     QFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDFTNFYEDLMNN
                     QKIPDSGVLTQKVKEQIADQLKQAGQ"
                     /name="traN"
     gene            complement(11557..12123)
                     /gene="trbC"
                     /locus_tag="H9194_RS25610"
     CDS             complement(11557..12123)
                     /gene="trbC"
                     /locus_tag="H9194_RS25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TrbC"
                     /protein_id="WP_241462864.1"
                     /translation="MNTPENRQFLKQQENLSRQLREKPDHQLKAWAEKQVLETPLQRSD
                     NHFLDELVRKQQASQDGKPRQGALYFVSFSIPEEGLKRMLGETRHYGIPATLRGMVNND
                     LKTTAEAVLSLVKDGATDGVQIDPTLFSQYGIRTVPALVVFCSQGYDIIRGNLRVGQAL
                     EKVAATGDCRQVAHDLLAGKGDSGK"
                     /name="trbC"
     Typing          complement(2351..5304)
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="94.124"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5305..6518)
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.765"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(9756..11560)
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.003"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11561..12123)
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.872"
                     /coverage="89.0"
                     /evalue="3.65e-120"
ORIGIN
        1 gtatttgcgg tattatcaaa atctggctgt gtcaggcact ccttccacaa atcccaggca
       61 gcacagcgtg catccagcgg attcaggatt ttatcgatgg agggatcata gtaactttta
      121 acaaattccc ccgaacggtc ataaatcacc accatatcac cacgctgacg ggcgtagttg
      181 gccagacgac ggataacctc cgacttaccg gccccgacag tgccgtgcag acagaagttc
      241 tggatttcag aatcccggat aatcggcaga tcgccaatcc ggatatcgga atccttgcca
      301 tcttttttca gcatcctggc cacctctttc ggattgtcag tcagctgacg accacctgtg
      361 atttcatttt cgctctgttg tttaccctga cgccccagaa tccaggagac aacaaagaag
      421 gtgatcaggc agataaccag ggccacgaca gacgccagaa cgaatgccga ccataactgc
      481 tcaccgcacc agatcatata tttatcatgc agtacctgag ccgcgttcat ccggaaggtt
      541 ttgccgtaat actggatctc atagaccggc tgggacctga ttaaatcccg catgccttcc
      601 agcgtggtgc accaccagta aatgcagcca ttcacaaacg tctgccagct tatttttacc
      661 cataaaacca gaccaactag gatccagaaa aaaataaaca ggcagtaaag tatgatattg
      721 gcgatctgac tgaacatgcg gatacgcatg gacgcaatct gaccgccctg ggtcatatcc
      781 tttgcgttaa aactcatgat gatattctga agaaagaaag tcagtcggac tgattattaa
      841 tgatgaaaag attaataacc gttaaattcc ggcttcgccg gcaacacttc acggtcgcac
      901 aaagaatttg cacaaccgct cagtgttttc atatttattt cggaaacgcc ggggagaaat
      961 aaaatttatt ttatttctcc ccggggttgg acaacgagca aagcgaggcg tcagtcagga
     1021 ggccggtcag accagcctcc ggaagataat cagagaatat ttgcgattga tttggccagc
     1081 tggtcttcga gaacaggctt cgcctcttca aatttcaggt taaccttgtt cgcatttgag
     1141 accacacggg tctggtattt atgctggtta cctgtttcag tactggtctg aattttcgca
     1201 cctgatgtgc cctgacgcag ggcggcaaca ttatccgttg tcaccgttgc cttagtacgc
     1261 tctgcaatct gcacatccgt gatcatggta tagttcacat cttccaccat cgcatctgca
     1321 gccataccca ccagaccggc agcaaggccc acaccgagtg tggcaccggc agaattggaa
     1381 ttatatccgg tgataccggc acctaacgct gcaccaactg ctgcgccttc ataaccacgg
     1441 ttcagccatc cctgagactc ccgcagatcc atcttatcgg ccttcagcac attcgcctga
     1501 atccagtagt aggctttatc cggagaagtc accacctgat acccttttgc tttcacagca
     1561 tcagcaattt tgccctgcag cccactcatg tctttatcag acgtgttttt gatctgcaga
     1621 aataccgtgc gttcgctggc gggttcaagc cagatggtct cactcatctg agtcttcacc
     1681 tcaaggttac gcttcttgat tgctgtgctc atcgcaccac accctgaaag ggccagagtg
     1741 gaactgacca gtgtaaccat catcaatttt tttgttttca tcaaattacc ttttttacaa
     1801 actacagttt tcttattgcc tgtcataata gtcctgccaa cgagtcccaa aagtatttcc
     1861 cgctgaatca aagcctccat gcataggata tccggttgaa gggttatagt agatcgttgg
     1921 ggtctgttca atgcccccga agtctaactt ttcactccca tcatattggt ctttgagttg
     1981 attttgcccc cattttttta tgcttatgca accactaact tgccttaatt cgtatagtgt
     2041 accaatgata aaaaccatca ggcttaataa cggagagagt aaggaagata ctggcatcgt
     2101 tatataccaa acgcctttac taaacttaaa ctcatccgac acatcccgtt tttttcgtat
     2161 ttgagagaac aaccagataa acagtccctg ccatagcact atgaagaaca gcataaaaac
     2221 tgtttcggca ttcccccaat cagccagata acaggcgata aactgtatca ctgttaccag
     2281 agcaatatgg gctaaacatc tcattatcat ctcccaaaca cagtattatt gcttatactt
     2341 atcctgatac tcctttgccc ttttcatcag ttcctgtgga ctgtcagccc cgggcattct
     2401 ttcctgagcc gacttttctt cattatattt attattctga gataatgcct ctgttttatg
     2461 gtgattctgc agttcagaat actgtcctct tacaatattt tcctcgccac gtatgctgtt
     2521 ctgagtatca cctatattcc ctttatattc tgtgaccata ttatcaacct gatgttttac
     2581 atcattacgg atattactgt cctgcgttct ttgctcaata atggcctgat gtccctgatg
     2641 gtcagcaata atatcctgac tgcctccacc cgaaggtacg ctctccattc ctttaccaat
     2701 atcgccacgg gattcccgcc aggcattatc aacaccaggc tgtatctgtt cctgcacaaa
     2761 agaccaggcc attgcccgac ggcgttctgc aatctccggc gaactggtat ttgtcagaat
     2821 agcttcggca tcctgcggtg cgtgtttcat cacatactgt gcaaactgtt gcgacaaatc
     2881 ttcgctcatc tgcccactca tgctttcagt acgggatgcc atttcagcat attcatggct
     2941 gcgggtcata ttcgtcgtgt actggtcgta actctgtttc gctgaattca gggctgcaga
     3001 cagttgatcc acccgggaat cagcattatt gtcagtatgg ctgccggatt cactgacttt
     3061 gcgactggta aagtaatcgc tggcctcttt aaagtcctta ctggctttag cgtcaatatc
     3121 atgacgagca tcatagctgg cccgtgaacc actgctggcc tgatgcgcat catcatcact
     3181 ccagtcaatg ccagcccgac caccacctct aacgcctaat cctccaccaa caccaagaat
     3241 tttaggttta accccccatt cagcatgagc atctccatac atcccggcag aagctctcgt
     3301 acttctggat gccagttcct gagtcgcctg ctcattactg atattatgcg ccttcgcata
     3361 gctttccact gcactgcgca tacggctggc catcatggag tcctgtgcac tcatcgtact
     3421 gtcagcacca ctggtgacag agtcactgct cccccggtta gaaccaaact gactgagcgt
     3481 gttccaggca ctggcaatac tgctgctgaa accatgcagg gcactttctg ctctgttcga
     3541 cgcctcccgg gccatttcct gttgtgccac tgcaatctga cgcgttgcat tgataccgac
     3601 cggtaaacgg gacatcgctc cgcttgcatc catcaccata ttaccgtcac gggtctgtgt
     3661 ggcggttgcg ccactgccgg tctggtacat catctgacca aacgacgtgg tgctgttggt
     3721 gctccagctg aagccgttca cgttttccgt ctgcatgttg ccgtaggagt aattaccgtc
     3781 aaccacacta cctgccgcac tggcagtcgg gctgatagca gaggaagcga aatggctgta
     3841 cacactggaa aatccggccc caagccccct gaccatcatc caggataatg gcgggatcat
     3901 catggaaaga tacccggcag tggaagccag atcggagtat ttcagttgta tctgtgaaat
     3961 ttcagagagc accaccggcg caccattctg tttcgcataa aatgtcatgg cactgttcag
     4021 aatggcgtac aagagtggcc atgactgcag ccacatcagg gcaaacacat aacctttcag
     4081 gacagacagc gtcagtttgt tgaatacagc cgccagtacc aggagcggga aaataccgat
     4141 ggcgatcccc gtcaggatgg tctgcgtcat gggcagtgtc cgcatcgcca catgaccgat
     4201 ggagacatgt gccagacgtt gtttctccat cgatgacgtg gtggccagat tcaccaggct
     4261 ggccgaatca ccattacgtg cggcataact ggtgatcccc tccttcagcg cattgatggt
     4321 gacattctgg cggatgatct ggctggctga tttacttgag ccatagaagt aactgtaact
     4381 gtcgccaatc agcgtactga acagcaggtt aggatcaggg cgtccaccaa atagctgctg
     4441 ggcataataa tgccaggttt ttccgccact ctgtgtgtcc agattcagct tgtctttcag
     4501 cgaaaccgac gcgtccttac aggttacgaa attattattg ctgtcataaa cgccccgcag
     4561 cgggctgggg cgggaaaaaa tcagcgtgta gggatcagcg gaggccatca gctcctccag
     4621 cgtgtatttg tggttcaggt aaatatcccc cataacacag ttctgcacat aatcctggaa
     4681 aagattggcg atttccggat tgcgggacaa aaagtcggtg ctttttgata ccagttctgc
     4741 gccaaacagc atccccgttt tgctgtaggt gacactgtcc ggttgcgtga agatcatctc
     4801 gtaactggcc accattgcat gcccgatacg ggtcgtcagt gaaagtggca tcgccagtcc
     4861 gaccggcaca ttatccaccc ggtgaacttt gaccaggtca ctgttatcaa taatctgcac
     4921 cgatgtgcgg acattaacca gcaggctgat aagcacaaat acggccaccc agcccagcaa
     4981 atccatcacg ttgtgccgct gtacccacat tacggccacc gcgagaacag acaatgtgag
     5041 cgcaattttt tcaatggaat cccacgtcct ggttcccata aaggcggcaa tggcgttcag
     5101 gttattccgc aaccactcac caccggcaat cacataaact tcattcaccg cgtgctccct
     5161 ccgaagtgat agttgttttg gtaacgactg agcatgcggg cggaaagctg ctgacgcatg
     5221 tagctcatct gacgatcgac aaccagcagc gcatcctgct gtacctgcac ctgtgactga
     5281 aacgccgcaa tctgccgggt ggcatcattc atgttgtcgt taatatgtcc gataaccgct
     5341 tcgtcataat ttcccgtggc caccatcgcc cgggcctgct gtatcagctc ctgaatgtac
     5401 tgcagcagga tgtcgtaacc gatatagtcc gtcagctggt aaatcatgct gttggaaact
     5461 ccgagcatct gcgggtcaac caggtatttg aagacgggga tggtggtact ggaaataaag
     5521 cctttttcct tgtcatccag aggcgtgtca ctgaccgcct tgttctgaat gctggccaga
     5581 agtttagtaa tctgagactt cagtgcatta tcccggctga tagtcaccgg tgtgtctgcc
     5641 accactttca ggcatttatc agaatcgttg cagtgataaa tttttgctgt gccgccttcc
     5701 atcatggccc ggataattga gcggtcagtg gttctggctg acagcggtgt gatttcgccg
     5761 ttgggaccaa acaccagtga gccggtcagc gtcatcacaa attctttcag ctctttgttg
     5821 ccgtcaaaca ttctgttttt ggaaagcgca ttccacatga tgttgatgtt tttggtcacc
     5881 cgctccttat ccttgtcgct ggctttatcc ctgacactgt cagatttccc gccaacggta
     5941 catccctgcc gggaagccgc ccagtcagca aaaatattgc tctcaccggc aatgtcctga
     6001 cagactttct gctgggacac ctgcgtccgg gggaaaagcc cgccgataat cccctgtgca
     6061 gcctggcagg aactgaggtt catactgtta atgtcgcttg ccattttctg cacgaagtct
     6121 tttgcggttt ttatttccgg caccgttgtc tgcagggcaa ggtcaaaaaa gtaaccggca
     6181 gcattgctca taatctgctt aacaaaacgc tgcaactgct caccattaat aaaactgaac
     6241 gagccgagat aggcgtcgat gccgccacat ccagcattga tatccggcag cgtcatggaa
     6301 attagctgaa catttttgac ctgagtacgg gcatacaggg agccaccgta agcatagccg
     6361 gcggcctgcc cctgccagac gcccggctgt gtggtattgg aagcaaatcc cagcttatta
     6421 aagaactgat tcatgtcgct gttcacatcc gctgacgctg caggcgtcac cgtcagtaag
     6481 gcggcacaaa gaaccagaag aggtttaatg cgtggcatca ttcttgccct cccttaatgt
     6541 gttctcccgg tgcatacgac atttcagcag gaatatctcg atatttccgt atattaattc
     6601 gtacattggg gacagaaaaa aatgtaacaa gaaacccggc tgcaagcgca tataacgcat
     6661 acggaatggc atatatcagg atattccaca tataactcga attatttaat tccgggtccg
     6721 ctttccacga atcaatacag gcatcaaaaa taatatccat aactgtccat aaaacaattc
     6781 cggcagtcag gaataccaga gacaacccag catacctgcc agtaattctg gaaatgtatt
     6841 tataatctcg ttctgtactt ctgattttga gctcaggatt acttttattt tctctcatta
     6901 taagttccct ctgatatttc cgatgattca ttcagaccgg tcatgcggct gtaatatatt
     6961 tcagacaccg aaatccatcc cacaattgcc cccacaaaaa acacaacaga aacagaaaag
     7021 gtaagactca gcgaaacgac aatttctaca gggctggcgg gataaaaaag cccctgacgg
     7081 ataaagggga tattcaggct gacgattatt cccgtcacga tgccggatat tccggcaatg
     7141 agtaacacca cctgcttatt tcgcaccttt ttttcctccg tacatctgca aaacggtatc
     7201 cacccgcgcc ataaaaccgg cggcatccgt tgccccctgt aaaagcggta atgcctcaag
     7261 cgtgttgacg ttgaccagga aggtggtcgg tgtggccacc gggatattcg ggaagaaggt
     7321 ctgcatcacc tccggtggca ccggtaatgc ttcaggaaag gccgtatccc cctgaccatc
     7381 cagggtgtag gaaaaaacgg aaaagccgta ctgctgtgcc agctgtttca gtaccgggtc
     7441 aaactggtga cagtaagggc aatgcccctg cataaacagc accaccttcc agtcggccag
     7501 gttaacctgc ctgccattac tgaggcggaa ccagcgggga gccggctttg ctgtcctggc
     7561 tgacgtgcct gctgccggtt gtgcaggctg cgtagccata ccctgcggat tccagagtcg
     7621 ctctatttca tcccgggtgg aggcctgcac agcagcggcg ctcagcaaca gggtgaacag
     7681 cagtgattta gtgagagaca tgtccgccct cctgctgccc ttccatcatg gcctgttgcc
     7741 tggcttccag tgcctcacgc tcttcccggc tgacacgggc aatccatgca tccagcacct
     7801 ggtatgcgcc gaacagtacg agaatggccg caatcagctc cgccagaaaa aaagccatcc
     7861 agggtttgct gtaaacaaga cgggcgacga tatgaaacca gacacccagg gaaaataccc
     7921 acattcccgt aatatcaagc cggggtaaag agaatctgcg tttacttatc atgttaaact
     7981 ccggttatta tttacatgag agtattcatg tctgtaatat tcgcgtttgt gtgatattaa
     8041 tcagaggtac aaatggaaaa gcgatctttt aaagaaaaac tggaaataat cagaaacata
     8101 ataagagagt cgttaccggg aaatgcggca attattgcgc tgatatatgc agcatcacat
     8161 tcattaccgg tcaatgcctt ccctgactat cttgtaataa gtttattaag tatcgcagca
     8221 ggcatcgttg tattatggtt attttcaatt atctatattt atttttgtga gctattcaga
     8281 agccactgga ttgcggtatg gtttatcata tggtcttcag taatcaacct cattattttg
     8341 tatggttttt atgaccgttt tatatgactt agcctgtcat aagccccctt ataaaaaacc
     8401 acatcactcc ggaccatacc gatgacaaca caatacagat aaatattctt ctgaacctgt
     8461 cgaagctcgt aacgtccggt aatgcggccg caactattga aacaaccagt gctgcgccct
     8521 gactcataat tgcaccacac agaaaccaga gactggcggt aagcacatat aaacacgcga
     8581 tattaataac aaggaaaacc agtgagctca ggagaaacat tttcctgtca ttacgctcag
     8641 aaaagaaata accggcaata atgacaaata ttaacagaac aacacctgta aacattttca
     8701 aataatcttc actcataaat caaatccgcg attaaaaatt gggtttaaaa tcttcagaaa
     8761 cgttcaggaa ctgttttgcc aggtcgtcct gcgaaataaa gccgtatgat aacgggcgaa
     8821 tactgccctg tttcgggtca accagcatca tggccgggaa atatttcacg ccgaggcgct
     8881 gcgcctgccc ctggtcagtc cgggaatccg gcaacagcgg attaatcacg ccatccacgg
     8941 aaacgggaat aacactcaga ccatacgtat cccggaagcc attaatgacc tgcgccagtt
     9001 gcccgtcgat ggggtcctgc ccccggtaaa aaaacatgat gccgtagtgt tcagccagtt
     9061 tcgcaatggc ctgtcgctgc tgcgcctggt ctgctgccag ctggttccgt accgtgccgt
     9121 tgtaatggct gtactgcagg ttatagtcca gttcaggatg tgccagcatg gcctttctgg
     9181 cgctcatggt gaaaagcccg gcctgctgag tccagtaatt ttgcagccgg aaatatttca
     9241 caaaattatc cacgccggga tacagaatgg cttcgtacag cgcccgcttt gttgccgtct
     9301 gcagtgcggc cagtttctgc ataatatccg gttcctgacg gggggctgca gatacaggtt
     9361 tattttcttt ttccttcgga tttattttct cgttatacca ctgccagcct gcatcttttc
     9421 ctgacgccgg aaaaataaag cagcagagta acagtggcag taatgcttta ttcatcttca
     9481 ttcctttcat cactttttct ttcatcaggt ttctgcaggt cagtcagggc agaaaaacaa
     9541 aaatacagaa taaacaggag cacaccggag taaaggataa cgaaagcagc ggcaagcatc
     9601 ccgtctgccg tggtttcttt taccgtatcc cacgaccaga taattaccgg agaaatgaca
     9661 atcgctgtaa tcaaaagccg gataagaaaa tcaataaaac ggttcgacgt gaatatgact
     9721 ttcatcattt cccccggtta tttcccatac cttactgtcc tgcctgtttc agctggtcag
     9781 caatctgctc tttcactttc tgcgtcagaa cgccactgtc cgggattttc tggttattca
     9841 tcaggtcttc gtagaagtta gtgaaatcca gtctgttgaa ctgaattttc tgcagctcat
     9901 caaccgtaat cccccgacag tcaggatgct tcgcactacc aaaactgata cgcaactgac
     9961 cgttgcgccc ctgctgctgg actatctgcg ccagcttcga atcaaactga cagtagctgc
    10021 gttttttctc cagacagaca cccagcactt tcttcgaaca gaactccccg acgctgacgg
    10081 tcagtttgtt tgattttgct ttagccaggg ccttttcatc actgctgcat ttggccagcc
    10141 cgatatcctg tccccagccg ctgtccttac agcagttgct gtatccggct gcagccttct
    10201 tacaaaattt cgcctgaccg gtgaaagcac ggacatcccc cccgttcagc gccgcaacat
    10261 ccttacctgc tgcggccagt gccgccagct gagaaacggc ttcggcaaaa tcattactct
    10321 gcccgctctg agccttatcg cattcgccat cgagacagaa tacgtctccc ccacagacca
    10381 tcactttccc ggatgttttt gactcacagg agtacgtggc atattcatgc agacaggcac
    10441 cttcttcgga gtaaaaggca cactggtgtg acaccagggt acaggcagga ttgtcggtgt
    10501 aggtctgaca ggttcccttg tctgctgact gcgtgacata cgtgtcccgg tatgcccagc
    10561 agctctggta catgctgtat gactgaccgt ctttcaccag cgtcctgttc ccgccagcct
    10621 ccgtacattc tgtttttgtc agtttcccct gctccttgct gaacgggcag ctttcagacc
    10681 agacaacata cggggtgtac gaggcttttt ttaccctgac aaccagacgc agcttcattg
    10741 ccgtatgacg tttgaatttt gtattcccga cccggacggg gtgagatgac gtaaaagcca
    10801 caccggcagt cagtgttttc tgtactggtg taaatgaggc atctgctgag tatttggcat
    10861 tccagctcag tggggttccc agtactgtaa ttgtcatagg tacactcttc tgcaagtaag
    10921 tacgactcca gctgtaatgc gcatcgacaa tttcaccggt aacgggagag gtaatggaga
    10981 caacatactg accattcact tcacgtgcag gtagctgact catttcatac tccagcgtcc
    11041 gggtttccca tgtggtactc ccctgcagct ccatacgggc agttcgtgtg cagtactgct
    11101 ccacctgtaa atcccgctca caggtgtaat tcgtgtattc actcctgcta atctcctgcg
    11161 cactgcactg ctgcccggtg tttcccacaa tgctgtcagc ccggttcacc acatccctgc
    11221 cggtctggat aaacggtgca tccggtgaaa gaatgtcttt cggcttgttc atgaatgact
    11281 ctgtaatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    11341 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    11401 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    11461 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagcc tgtgccatgc
    11521 cggcaaccag agccagtatc agaggtaaaa tacgtttcat ttcccggaat ctcctttccc
    11581 tgccagtaaa tcatgagcca cctgccggca gtcacccgtg gcggccactt tctccagcgc
    11641 ctggccgacc cgcaggtttc cccggatgat gtcgtatccc tgactgcaga acaccaccag
    11701 tgccggtaca gtgcgaatgc cgtactgtga aaacagcgtc gggtcgatct gaacaccatc
    11761 agtcgctccg tctttcacca gggacaacac agcttcagcg gtggtcttca ggtcattgtt
    11821 caccatgccc cgcagtgtgg ccggaatacc gtagtgccgg gtttcgccca gcattcgttt
    11881 cagcccctct tcgggaatgg aaaacgacac aaaatacaga gcaccctgcc ggggtttccc
    11941 gtcctgcgaa gcctgctgtt tacgaaccag ctcatccagg aaatggttat ctgaacgctg
    12001 aaggggggtt tccagcacct gtttctccgc ccaggctttc agctgatggt caggtttttc
    12061 acgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt caggagtgtt
    12121 cacg
//
