LOCUS       NZ_CP060962             1458 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC8 plasmid pEC8-10, complete sequence.
ACCESSION   NZ_CP060962
VERSION     NZ_CP060962.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904652
            Assembly: GCF_022559245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559245.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 89 of 238
            Pseudo Genes (incomplete)         :: 170 of 238
            Pseudo Genes (internal stop)      :: 36 of 238
            Pseudo Genes (multiple problems)  :: 51 of 238
            Pseudo Genes (short protein)      :: 1 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060962.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC8"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC8-10"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-18"
                     /collected_by="Long Sun"
     gene            1..585
                     /locus_tag="H9195_RS26435"
                     /old_locus_tag="H9195_26180"
     CDS             1..585
                     /locus_tag="H9195_RS26435"
                     /old_locus_tag="H9195_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="WP_241466774.1"
                     /translation="MCKCNTVTRDDIPFAIWSELRPDTPYQCVRAQCGFLFSSASSPTR
                     SLTFGRWGCGERCHLSHAVGDSAVVLSGMSRQIALQTRSEALRRTRRGLNARQGMEGAK
                     NAYRAFQEVRRADTPSQGLSGRQEPRQPPAKPICTVQAVTRLVGGRRPSVLQRWGSGVL
                     PRRFPVALKALPEPWRLGRSGDRGAKTRKGF"
                     /name="rep protein"
     gene            complement(670..1110)
                     /locus_tag="H9195_RS26440"
                     /old_locus_tag="H9195_26185"
     CDS             complement(670..1110)
                     /locus_tag="H9195_RS26440"
                     /old_locus_tag="H9195_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagagctt
       61 cgccccgaca ccccgtatca gtgcgtacgt gctcaatgtg gtttcctgtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgctg tcggtgattc cgctgttgtt ctttcgggga tgagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aggcggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccccctg caaagcccat ttgcacggta
      421 caggcagtca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 ggtcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaatccac tagaaattgc atgcaatgtt ctgtggattg tccgcctgcc
      661 gctggctctt taaatccggc cgccctgaat gacactaaag ccgtttttga gtgccttttt
      721 gtggttggtt acggtgcctt tccaaccaaa ttgttcattc agccggtatg tttttgatct
      781 gccgacttta ggtccctcaa agatcacccc aacatccagt agcgcgcgaa cggcgcgact
      841 aacattcgtt ttctgcatcc ctaaagtttc agcaatatct gcttgggcga tctgaatgaa
      901 gttttcgtaa tccagatctg caagcatcag cattaagacc ttgaattgct ccccggttaa
      961 ttccttagcc aagatccgta atgcgtcttg attcatggtg aaatgcctct gaaatgctga
     1021 tttttgttta gggcggataa cggcaacgaa gccgccgata atctcaccct ggtcatttat
     1081 ctgagtgatt ctctcgacat ccttactcat ctttgaaccc tatttgagtt atcaataatg
     1141 ataacagtgt tatcattatt gataactatg tcaactgtaa cccttagatt ttattaatgt
     1201 ttttattctc ctttctgtat ctttatatta ggcgtctaac gcattgattc cattagatat
     1261 ttatcctagc tgctctttgg ggtgtcgggg cgaagccctg acccagtcac gtagcgatag
     1321 cggagtgtat actggcttac tcctctgcaa gcctcgtcag ccatgatttt tgtccgattt
     1381 ctcgctctcc acgttcaaca gctccatcat gtttttgccg tccgttcacc gttaaaacgc
     1441 ctcagaaatc aggacagg
//
