LOCUS       NZ_CP060960             2113 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC8 plasmid pEC8-8, complete sequence.
ACCESSION   NZ_CP060960
VERSION     NZ_CP060960.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904652
            Assembly: GCF_022559245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559245.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 89 of 238
            Pseudo Genes (incomplete)         :: 170 of 238
            Pseudo Genes (internal stop)      :: 36 of 238
            Pseudo Genes (multiple problems)  :: 51 of 238
            Pseudo Genes (short protein)      :: 1 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060960.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC8"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC8-8"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-18"
                     /collected_by="Long Sun"
     gene            join(2036..2113,1..888)
                     /locus_tag="H9195_RS26415"
                     /old_locus_tag="H9195_26160"
     CDS             join(2036..2113,1..888)
                     /locus_tag="H9195_RS26415"
                     /old_locus_tag="H9195_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            892..1158
                     /locus_tag="H9195_RS26420"
                     /old_locus_tag="H9195_26165"
     CDS             892..1158
                     /locus_tag="H9195_RS26420"
                     /old_locus_tag="H9195_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          317..548
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gtcgggggta tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc
       61 tgtagcggtt tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta
      121 cggcttcgta gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga
      181 accctcatgt ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg
      241 tcttcccgat ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga
      301 acagtcctta cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca
      361 cctgttcagg ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat
      421 ccgcattttc actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt
      481 aagcacgaac gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat
      541 atcgatattc gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa
      601 ctgcaaagcg cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac
      661 gatcctgagt ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgacc
      721 ggtggggcgc taaaaaacgt cctccagttg gatcgagaaa ccaatgagga tcttgtcatt
      781 gccgacgatg taggggatgg cactgatgac gggaagcgga cggcgtttgt ctgggattca
      841 ggtaaacggc gttacaaacg cgcccctgag aaggataaat cggattaacg tatgaatatt
      901 aatattgaat acctgaatgg aaataagact attggtttat tttttttaag aagtgaagcg
      961 gtgattcctg acaggtttaa aaaccttatt ttgcttattg atggattaag ttttggcaca
     1021 tttggttttc atccgcacga aggttttgag gatgaattaa ttttatatat tcagaaaaca
     1081 aacgagaggg taaaaactct ttttgtgaaa atagatttaa acggtatcaa gtttggattt
     1141 ttaagaacgc attcttagtt ctggaaaaga gccagcggca ggctgaggtg ataggtacga
     1201 gattgcatgc aatctctagt gctctgtcta tcctgcatta tcctcagcat tatcctcagc
     1261 attatcctca gcattatcct cagccttgcc aactcgacac caatgcagga tagacaatcc
     1321 gatgtcaaat gttaacactc tgcgagtggt acattttccc cggattatcg tcctgagcct
     1381 gccgctggct ctctttctac cgcctcgctt tgctcgttgc tcaacgcctc acagacacgg
     1441 attaaaatcc gcatccgttc accgtttttt aaagtccgtt aaaagcatga tgccatctcc
     1501 gagagttaat ctcgtcaaat gctaaatcgt gggggtcccc tttggggttc cgatttagtg
     1561 attgacgaca ccaccgatta aaaaacttat gcggggtgga tggtttcacg aagtgaggcc
     1621 atccacctgt aagacagggt tttgttttta ttccctgttt tggtgatcgg gtgtgtggaa
     1681 aaggttgggg taagccgttc gggggtgctt gttttggggg gttaaaattg tggttatttt
     1741 ttgcgcaatt ctcgcgcgtg atccttgtat ttatacttaa gggataaatg gcggatatga
     1801 aatagtggtt tagcccagta atgacgaggc tttgagtggg ttttgacagg tcaaagaaaa
     1861 tggagcagaa ttgaggcgtt tttaatcggc gttggggagt gcgtcaacac tccccaacat
     1921 ttcgaatgtg tcacctcagc ggcaaactct ggtgacatgt actggctcgc aatgcacagg
     1981 tacgtgatga atataccaca tcaaatcaca gcctgcccag atcggagcag gcttaatgtc
     2041 agaagataaa ttcctttcgg actacagccc ccgtgatgca gtttgggata cccagcgcac
     2101 gcttaccgat tct
//
