LOCUS       NZ_CP060959             4072 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC8 plasmid pEC8-7, complete sequence.
ACCESSION   NZ_CP060959
VERSION     NZ_CP060959.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904652
            Assembly: GCF_022559245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559245.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 89 of 238
            Pseudo Genes (incomplete)         :: 170 of 238
            Pseudo Genes (internal stop)      :: 36 of 238
            Pseudo Genes (multiple problems)  :: 51 of 238
            Pseudo Genes (short protein)      :: 1 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060959.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC8"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC8-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-18"
                     /collected_by="Long Sun"
     gene            complement(join(2899..4072,1..986))
                     /gene="mobQ"
                     /locus_tag="H9195_RS26400"
                     /old_locus_tag="H9195_26145"
     CDS             complement(join(2899..4072,1..986))
                     /gene="mobQ"
                     /locus_tag="H9195_RS26400"
                     /old_locus_tag="H9195_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_241466773.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            1165..1419
                     /gene="mobC"
                     /locus_tag="H9195_RS26405"
                     /old_locus_tag="H9195_26150"
     CDS             1165..1419
                     /gene="mobC"
                     /locus_tag="H9195_RS26405"
                     /old_locus_tag="H9195_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1951..2643
                     /locus_tag="H9195_RS26410"
                     /old_locus_tag="H9195_26155"
     CDS             1951..2643
                     /locus_tag="H9195_RS26410"
                     /old_locus_tag="H9195_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(629..987)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.611"
                     /coverage="100.0"
                     /evalue="1.82e-180"
     Typing          988..1164
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1952..2643
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1073)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.44"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2903..4071)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.744"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc ctttcgttcg
       61 gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat cgacatggca
      121 ttactggtga gtttccatgc cgcattactg tccttcggca acgcttcgat gatgtcacgt
      181 agtgtgtacg aagttgcgtt gtcaggaagc acgatattca gaaggtcagc cacacggcgg
      241 gcaggctctg tcagcccgct gttcctgagt tcagtgcggg aaatctgtat ttccaggtta
      301 agcctgttaa tcagacgatt ctgttcgccg cgttctgttt cgataccgcg tgtttccatc
      361 gcacaggcag actttccgag gtgaatacca ggcgttttct caagcccctg tcgctcgtat
      421 gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gtgctctgtt tgcatggtca
      481 gcccatgatg cacgccacgt ctcggcatgt gtccggtcgt tccagtccct gacctttccg
      541 ccgaacccag caggtccgac agccctcgtg gtcagcatga tgtgcgcatg gggatttgtt
      601 ttatccatgt gatggaacgc cacatcagca atcatgccct gactgacaaa gttttctctg
      661 acaaaggcga gaacggtttc ccgtgcggca tcctgcggca gttcacgggg gatagcgagt
      721 tcaatttccc gcgcaagttg tgagttcttc cggcattcgg ccttctcaac ggcattccag
      781 agttcggcac ggttcgtaca ccaggcaggg gctgtcgcgg gggtcagaat cacagcttca
      841 gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc aagcatcagt
      901 gaaccggaac gataggcagc agaagcaacg gcgctgtagc cgctgtttct ggagatgatt
      961 ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgtgg ttttctttca
     1021 gccccgcagg gcgcacacaa tcgaaacgga gtggagattg tataagtgcg cacttcgtga
     1081 ttttccgtgc attatgccag ctcagttttt aaactaccag tgacgcgcta aaatgcgatt
     1141 tttcatcgcg cacggagtta catcatgagc agaacactcg aacagaagat tgctgatgct
     1201 gaggccagat tacagcgcct gaaggcgaag agcaggagtc tggacacagc gcagaaggtc
     1261 attgtgggtg cagcattgct ggcaaaggtc agaaagccgg aggaggtgca gttgcgtgcc
     1321 tggttactcc agttcctgaa ggcagaggtg acacgacagg ctgatgtgac gcgcatactg
     1381 ccgctgatta acgagctgga ggcgctgcca ggacagtgat tgtatcgtcc gctcgccgca
     1441 gccccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga atccgtcgcc
     1501 aggttgagca catacgcacg gtttttcggt aggtgatatg cgctgaaccc gcatgaatac
     1561 tggatttcca cagggttgga aggggagtat tcggaacttg caattctgct ttcttgcaac
     1621 acattgtttt tattggtttt ttgttttcag cgccccaagt tatccacagg ctcgccgtta
     1681 agtttttaga tcttgttttt aagatcttgt ttttaatact tgttttaggg tcaaaattca
     1741 ttaataaaat caatatgtta tagaggtata tgtggaggaa tttaaggtaa aggtggagga
     1801 atttaaggtg tttaggtgga ggaatttaag gtaaaggtgg aggaatttaa ggtgttattc
     1861 acagaaatgt gaggatataa aaaaaaatat tccacatttc ttatttgtgg ggcacattaa
     1921 agcatcacca catttgaacg ggatctttgt atgtcggact tgattgccta caagtcgaat
     1981 gctttggttg aggccagcta caagttgacc ctacaggaac aacgttttct gttgctgtgt
     2041 atcagtcgat tgaaatctgg tgctgatgct gaattgcaaa agacaatgac cattaccgct
     2101 gcggaatatt ttgattcatt ccctgacatg gggcggaaaa atgccgaggt tcagttacag
     2161 gaagcgatcg atcgtttatg ggacaggtcg atcattctca agaatgacga gaagcgtgag
     2221 gagttccgct ggatccagta tcgggcacag tacgctaaag gtgaggccaa ggctcgaata
     2281 accttttctg acgcggtaat gccttatctg acgcaactga aggggcaatt cacgcgcgtt
     2341 gttataaaaa acatatcagg tttatcaagt agttacagta taagaatata cgagttcctc
     2401 cagcaattcc gcagtacggg agagagaatt attgctctta atgactttcg ttcaatgtta
     2461 gggattgaaa ataaatacaa acaattcaga gacttaaata aaatactaat aaaaccctgt
     2521 gttgacgaac tgaacaaaaa aagcgacctg gctgtaacgg ttgagacgat caaaaagggg
     2581 cgcacggttg tagccctgca ttttcgcttc aaggaagaca aacagatcaa gatgacgatc
     2641 taaaatcgat tttaaggcac ctaaaacgct ccctgctgcg ttttttttgt gttagtagtg
     2701 cgaaggtact attgaagtga aaaattgcgt caaaagcgct ctaatgcagc gaaaaaagaa
     2761 cccccaattt ttgggggcgg aaaaacaaaa ctatgcttgc attatgtgtg tgcttcttgc
     2821 attcatccct gtcgatgaac atagaagaac atcgacgaac ataacacgat gttaaacaaa
     2881 acaaaatatt gttcacattc actgtgacca accgcgatca tgtctcctct cggtggtgtg
     2941 tgttcttgca tcttcctcgc gccgccattc atcccaggta cgattgaaac ccagagagcg
     3001 gaacagatga tccggctgtt tttctggtgt gttgacggca gcaaaaagcg gtgcctcacg
     3061 ggttttcaga tgaaactcca taagttccgg cgcaggtgat atgtggctgt ctgcatcgtg
     3121 atttacggca acaccatacc ctgcataccg taacccctta tcggtcagtg cactgatgac
     3181 cagctggctg ttatcggctg agggaacggc aaacgcggga tacagcgtac cattaagccg
     3241 gacaacaccg cccagagagt catccacgcc catgaccagc aaaagctgaa gctggcgggt
     3301 ttcccggtcg acagaactca ttaccggcat cagcgtcggt agcagacgtc cctgcgtaaa
     3361 atccaccttc cttgctgcca gtgatggcat ggctggctgg cggtcttctc caagacagaa
     3421 agactccggc catgtcttcg gctggcggag attgcgggta tattgcggcc atgtctggca
     3481 aacctgcgtc agaagttgct caaaggtcgc agaggaataa acacgttgca gtggtggatg
     3541 ttgaccgatt tcgcttcggt cacagatata accactcctg tcacgcatta aatcaacacg
     3601 ctcaaagatt ctggtgcggt atttctccag ctcgttcccc cagagcgtgt attctccgtt
     3661 ccggttgtga agcagaaggt aacaggtcat ttccccgtta ccaggcatgg gggcctcgcc
     3721 ataccccgta acgactgcgc tcagtacccc tgaatccata acggcattgt catttttcct
     3781 gagctgttcc gctcgtcgct ccgcgcgact acgttctgta tcctcctgct ccttccggcg
     3841 caattcctct tcacgtttaa gcaactggac tctgagagct gcaaggccgg atgcccccca
     3901 ctgctgccac tgtttttcaa atgccgtctg actcgcggga atgtcctgtt tctccacctt
     3961 ccggtaatgc tgtcgcaatt tttccatttg ctggctcagc gactggattt ttccctgttc
     4021 gctacggtat tccggtgccg cccattgcat caggggaata cgggaaaaac cg
//
