LOCUS       NZ_CP060958             4087 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC8 plasmid pEC8-6, complete sequence.
ACCESSION   NZ_CP060958
VERSION     NZ_CP060958.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904652
            Assembly: GCF_022559245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559245.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 89 of 238
            Pseudo Genes (incomplete)         :: 170 of 238
            Pseudo Genes (internal stop)      :: 36 of 238
            Pseudo Genes (multiple problems)  :: 51 of 238
            Pseudo Genes (short protein)      :: 1 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060958.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC8"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC8-6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-18"
                     /collected_by="Long Sun"
     gene            complement(join(2924..4087,1..996))
                     /gene="mobQ"
                     /locus_tag="H9195_RS26385"
                     /old_locus_tag="H9195_26130"
     CDS             complement(join(2924..4087,1..996))
                     /gene="mobQ"
                     /locus_tag="H9195_RS26385"
                     /old_locus_tag="H9195_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            1175..1429
                     /gene="mobC"
                     /locus_tag="H9195_RS26390"
                     /old_locus_tag="H9195_26135"
     CDS             1175..1429
                     /gene="mobC"
                     /locus_tag="H9195_RS26390"
                     /old_locus_tag="H9195_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1961..2668
                     /locus_tag="H9195_RS26395"
                     /old_locus_tag="H9195_26140"
     CDS             1961..2668
                     /locus_tag="H9195_RS26395"
                     /old_locus_tag="H9195_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(639..997)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          998..1174
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1962..2667
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..1083)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.892"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3387..4087)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="83.0"
                     /evalue="3.09e-141"
ORIGIN
        1 ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc
       61 ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat
      121 cgacatggca ttactggtga gtttccatgc cgcattactg tccttcggca acgcttcgat
      181 gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc acgatattca gaaggtcagc
      241 cacacggcgg gcaggctctg tcagcccgct gttcctgagt tcagtgcggg aaatctgtat
      301 ttccaggtta agcctgttaa tcagacgatt ctgttcgccg cgttctgttt cgataccgcg
      361 tgtttccatc gcacaggcag actttccgag gtgaatacca ggcgttttct caagcccctg
      421 tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gtgctctgtt
      481 tgcatggtca gcccatgatg cacgccacgt ctcggcatgt gtccggtcgt tccagtccct
      541 gacctttccg ccgaacccag caggtccgac agccctcgtg gtcagcatga tgtgcgcatg
      601 gggatttgtt ttatccatgt gatggaacgc cacatcagca atcatgccct gactgacaaa
      661 gttttctctg acaaaggcga gaacggtttc ccgtgcggca tcctgcggca gttcacgggg
      721 gatagcgagt tcaatttccc gcgcaagttg tgagttcttc cggcattcgg ccttctcaac
      781 ggcattccag agttcggcac ggttcgtaca ccaggcaggg gctgtcgcgg gggtcagaat
      841 cacagcttca gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc
      901 aagcatcagt gaaccggaac gataggcagc ggaagctacg gcgctgtagc cgctgcttct
      961 ggagataatt ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgtag
     1021 ttttctttca gccccgcagg gcgcacacaa tcgaaacgga gtggagattg tataagtgcg
     1081 cactttgtga tttgtcgcgc attatgccag ctcagttttt aaactaccag tgacgcgcta
     1141 aaatgcgatt tttcatcgcg cacggagtta catcatgagc agaacgctcg aacagaagat
     1201 tgctgatgct gaggcgagat tacagcgcct gaaggcgaag agccggagcc tggacacagc
     1261 gcagaaggtt gttgtgggtg cagcattgct ggcaaaggtc agaaagccgg aggaggtgca
     1321 gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg acacgacagg ctgatgtgac
     1381 gcgcatactg ccgctgatta acgagctgga agcgctgcca ggacagtgat tgtatcgtcc
     1441 gctcgccgca gtcccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga
     1501 atccgtcgcc tgattgagca catacgcacg gtttttcggg aggtgatatg cgctgaaccc
     1561 gcatgaatac tggatttcca cagggttgga gggggtgtat tcggaacttg caattctgtt
     1621 tttttgcaac gcattgtttt tattggtttt ttgttttcag cactccaagt tatccacagg
     1681 ctcgtcgtta agtttttaga tcttgttttt aagatcttgt ttttaatact tgttttagcg
     1741 ccaaaattcc ttatagaaaa caaaatgtta caacgctata ggtaatatga tttaaggtaa
     1801 aggtgatata atttaaggtg cttgggtgag gtaatttaag gtaaaggtga ggagatttaa
     1861 ggtaatattc acaggaaaat ggggggtgaa aatataattg ctcaccaatg ctaaaggtga
     1921 taggttttta tttcacctca tttttggcct ggtggttaaa atgacatcaa accctctgat
     1981 agcctataag tccaatgcgc tggttgaggc cagttacaag ctaactttac aagaacagcg
     2041 tttcttattg ctctgcataa gccgacttaa ctccggtact gacgtagctt cacctgaatt
     2101 gcaaaagaca atgaccatta cagctgcgga atattttgat tcattccctg atatggggcg
     2161 gaaaaatgcc gaggttcagc tacaggaagc gatcgatcgt ttgtgggaca ggtcgatcat
     2221 tctcaaggac gacgagaagc gcgaggagtt ccgctggatt cagtatcggg cacagtacgc
     2281 gaggggagag gggaaagcac aaatcacttt ttctgacgcg gtaatgcctt atctgacaca
     2341 gttgaagggg caattcacca gagtcgtcat taaaaatatt tctaatttat caagaagtta
     2401 cagcataaga atatacgaaa ttctccagca attccgcagt actggggaac gtattatcgc
     2461 cctcgatgat tttaaatcat cactgatgct tgatggtaaa tataaagatt tcaaaacgtt
     2521 aaacagggat ctaataaaac cctgtgttga cgaactgaac aaaaaaagcg acctggctgt
     2581 aacggttgag acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc gcttcaagga
     2641 agacaaacag atcaagatga cgatctaaaa tcgattttaa ggcacctaaa acgctccctg
     2701 ctgcgttttt tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa
     2761 gcgctctaat gcagcgaaaa aagaaccccc aatttttggg ggcggaaaaa caaaactatg
     2821 cttgcattat gtgtgtgctt cttgcattca tccctgtcga tgaacataga agaacatcga
     2881 cgaacataac acgatgttaa acaaaacaaa atattgttca cattcactgt gaccaaccgc
     2941 gatcatgtct cctctcggtg gtgtgtgttc ttgcatcttc ctcgcgccgc cattcatccc
     3001 aggtacgatt gaaacccaga gagcggaaca gatgatccgg ctgtttttct ggtgtgttga
     3061 cggcagcaaa aagcggtgcc tcacgggttt tcagatgaaa ctccataagt tccggcgcag
     3121 gtgatatgtg gctgtctgca tcgtgattta cggcaacacc ataccctgca taccgtaacc
     3181 ccttatcggt cagtgcactg atgaccagct ggctgttatc ggctgaggga acggcaaacg
     3241 cgggatacag cgtaccatta agccggacaa caccgcccag agagtcatcc acgcccatga
     3301 ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga actcattacc ggcatcagcg
     3361 tcggtagcag acgtccctgc gtaaaatcca ccttccttgc tgccagtgat ggcatggctg
     3421 gctggcggtc ttctccaaga cagaaagact ccggccatgt cttcggctgg cggagattgc
     3481 gggtatattg cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag gtcgcagagg
     3541 aataaacacg ttgcagtggt ggatgttgac cgatttcgct tcggtcacag atataaccac
     3601 tcctgtcacg cattaaatca acacgctcaa agattctggt gcggtatttc tccagctcgt
     3661 tcccccagag cgtgtattct ccgttccggt tgtgaagcag aaggtaacag gtcatttccc
     3721 cgttaccagg catgggggcc tcgccatacc ccgtaacgac tgcgctcagt acccctgaat
     3781 ccataacggc attgtcattt ttcctgagct gttccgctcg tcgctccgcg cgactacgtt
     3841 ctgtatcctc ctgctccttc cggcgcaatt cctcttcacg tttaagcaac tggactctga
     3901 gagctgcaag gccggatgcc ccccactgct gccactgttt ttcaaatgcc gtctgactcg
     3961 cgggaatgtc ctgtttctcc accttccggt aatgctgtcg caatttttcc atttgctggc
     4021 tcagcgactg gatttttccc tgttcgctac ggtattccgg tgccgcccat tgcatcaggg
     4081 gaatacg
//
