LOCUS       NZ_CP060956            11534 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC8 plasmid pEC8-4, complete sequence.
ACCESSION   NZ_CP060956
VERSION     NZ_CP060956.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904652
            Assembly: GCF_022559245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559245.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 89 of 238
            Pseudo Genes (incomplete)         :: 170 of 238
            Pseudo Genes (internal stop)      :: 36 of 238
            Pseudo Genes (multiple problems)  :: 51 of 238
            Pseudo Genes (short protein)      :: 1 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060956.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC8"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC8-4"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-18"
                     /collected_by="Long Sun"
     gene            join(10732..11534,1..487)
                     /locus_tag="H9195_RS26285"
                     /old_locus_tag="H9195_26030"
     CDS             join(10732..11534,1..487)
                     /locus_tag="H9195_RS26285"
                     /old_locus_tag="H9195_26030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241466771.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLSLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            484..1125
                     /locus_tag="H9195_RS26290"
                     /old_locus_tag="H9195_26035"
     CDS             484..1125
                     /locus_tag="H9195_RS26290"
                     /old_locus_tag="H9195_26035"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_234278848.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGTELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1145..1330)
                     /locus_tag="H9195_RS26295"
                     /old_locus_tag="H9195_26040"
     CDS             complement(1145..1330)
                     /locus_tag="H9195_RS26295"
                     /old_locus_tag="H9195_26040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162780107.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRREQPRSTGR"
                     /name="hypothetical protein"
     gene            complement(1323..1685)
                     /locus_tag="H9195_RS26300"
                     /old_locus_tag="H9195_26045"
     CDS             complement(1323..1685)
                     /locus_tag="H9195_RS26300"
                     /old_locus_tag="H9195_26045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186829.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            complement(1678..2250)
                     /locus_tag="H9195_RS26305"
                     /old_locus_tag="H9195_26050"
     CDS             complement(1678..2250)
                     /locus_tag="H9195_RS26305"
                     /old_locus_tag="H9195_26050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241466772.1"
                     /translation="MSAFKLLCDRLRNEATLAKSDGAGAFKKQSLDPDLKGGKKGKKPA
                     GELEEDEAIASLHRKRMNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAP
                     IGAPVASPTEAVHRACAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAP
                     AFAEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            complement(2250..2909)
                     /locus_tag="H9195_RS26310"
                     /old_locus_tag="H9195_26055"
     CDS             complement(2250..2909)
                     /locus_tag="H9195_RS26310"
                     /old_locus_tag="H9195_26055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599772.1"
                     /translation="MTEIKKESADDAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            3169..3756
                     /locus_tag="H9195_RS26315"
                     /old_locus_tag="H9195_26060"
     CDS             3169..3756
                     /locus_tag="H9195_RS26315"
                     /old_locus_tag="H9195_26060"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_064186842.1"
                     /translation="MNKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            complement(3837..4091)
                     /locus_tag="H9195_RS26320"
                     /old_locus_tag="H9195_26065"
     CDS             complement(3837..4091)
                     /locus_tag="H9195_RS26320"
                     /old_locus_tag="H9195_26065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_187361968.1"
                     /translation="MLLTPVRLSLWSRLPCVKADDADGAVSPCLVAEGSLVLASAVGGA
                     MTRLAKLADLAVFDAQARVAGRGGLLYALHVIKPLTGGW"
                     /name="hypothetical protein"
     gene            4177..4422
                     /locus_tag="H9195_RS26325"
                     /old_locus_tag="H9195_26070"
     CDS             4177..4422
                     /locus_tag="H9195_RS26325"
                     /old_locus_tag="H9195_26070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239568.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTLANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            4620..5087
                     /locus_tag="H9195_RS26330"
                     /old_locus_tag="H9195_26075"
     CDS             4620..5087
                     /locus_tag="H9195_RS26330"
                     /old_locus_tag="H9195_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5779..7302)
                     /locus_tag="H9195_RS26335"
                     /old_locus_tag="H9195_26080"
     CDS             complement(5779..7302)
                     /locus_tag="H9195_RS26335"
                     /old_locus_tag="H9195_26080"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_234278846.1"
                     /translation="MSNHEENSGAAVATVTQIAAAKAKAAAKTERPAFMGGSLPPVPFN
                     EWDGLTGNPTRDGFRIQAAEKRSINFGNEYPLRYVPPLISDAGTGRWDVLNHADNLRWL
                     LYRSGARMELNLMTFKNELSWADSGSLVEPDEHGARAWILNKSAEVGLKRQAVDDFMFY
                     VARSAPYHPVARLLGSREWDGSPRVDAVLDCVPVAPGKEALRSALLRGLLIAAITAVEE
                     GAVSNKYVPCLYSEANNWFKTAFFRALFDLLPGAFGQGVSINPSSKDSVRPAVMRWFNE
                     LGEVSGMMKKNDPDEIKAFVERNVDEWRPENEKAVIAKKRQMVMGATVNKRDYLKDETA
                     ASRFPTITLVAPIDIARVNEVLGWEWDNVKGCAVRADEDQLIQFWLEVRHLYRSGVGFN
                     LDDETFAAAKAETAQFVDRGSYYEPVRDILLLPGNASTGQYPWDKPGYDGRFTATDVTA
                     ALRAPSSHSGRVGRALKRLADEGIVDRRNHRGTFVYWKLPDEPTGELPL"
                     /name="VapE domain-containing protein"
     gene            7813..8301
                     /locus_tag="H9195_RS26340"
                     /old_locus_tag="H9195_26085"
     CDS             7813..8301
                     /locus_tag="H9195_RS26340"
                     /old_locus_tag="H9195_26085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186838.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQTARQSAPQNARQIVPRGADPRFQ
                     AVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERGLI"
                     /name="hypothetical protein"
     gene            complement(8319..8777)
                     /locus_tag="H9195_RS26345"
                     /old_locus_tag="H9195_26090"
     CDS             complement(8319..8777)
                     /locus_tag="H9195_RS26345"
                     /old_locus_tag="H9195_26090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599770.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     IVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(9008..10180)
                     /locus_tag="H9195_RS26350"
                     /old_locus_tag="H9195_26095"
     CDS             complement(9008..10180)
                     /locus_tag="H9195_RS26350"
                     /old_locus_tag="H9195_26095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241466770.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDTARQLYVDGLEAICGRCS
                     AEGFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPVPTI
                     PGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            complement(10167..10376)
                     /locus_tag="H9195_RS26355"
                     /old_locus_tag="H9195_26100"
     CDS             complement(10167..10376)
                     /locus_tag="H9195_RS26355"
                     /old_locus_tag="H9195_26100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
ORIGIN
        1 ctggacagcg tcggtctgct ccgattgcaa agccgcgccc ctggtgatca gcaaggcacc
       61 ggtcaagggc aagccgcgac gggagatcgt cgtctcctgc ccgtcctgcg actccagcgc
      121 atcagccagg cccggaggca ggacatggac agacgagcac atcgcggtgc ccgagttcac
      181 gcagaacgag acgcgcagcc tggcgaagat ccccgttatt ttgccgcaga gggagcagcc
      241 gcagctgctc aaaagaccag ggacagggcg cggtccagga cgcccccgca agcgcatccc
      301 cctgaccctc gaccgggtac cagagagtcg gttcaaccgc ctctcccttt ccgtccttgc
      361 cgccgtcggg cgctgggact acatcaacga catcatccgg cgcatcgatc gggaccgcgg
      421 cgacgccgcc accctgatcg cccttgccga ccacatccag accaacagac ctgagagaga
      481 tttatgaccg aacccacccg cccgatcctg accatcaagc gacgcaaccg cacggtctac
      541 gtcccgccgg aaaaggccgc gaaatctgag ccacagccag ccgaacggac taccagcaag
      601 gcgaaggtac agccaaaagc gaaagccccg cagaagcccg cacagaagcc cacagcggca
      661 aagccgaaaa agacgcccga gcaggtcgcc gaggggatcc gcagacagga ggagaacgac
      721 cgccgccagc aggacatcgc gatcgccaac aagtccaaaa aggcgctggc caaccgggtg
      781 accaaagcat tcctgaaaga gcactggcct gacttactga cccggggagt tgaccgtcgc
      841 cccatggcaa agggcatcat cgagcagatc ctgacacgag gcacagagct ggacatgccg
      901 ccagaggcag accgcaaagc cgtccgtcgc atcctgggca ttgtcacggg taccagctcg
      961 taccagcaga gcctcatcga ccagtgcatg cgctacgacc tggacggcaa ccccaccgaa
     1021 ccagcaaccc cggaggaggc cgaaaacgcc aaagcccgcc tgacagagat caaggcattc
     1081 cggcgcaagg cacgccaggc gaagggcgaa aaaaagggcg cataacgcgc cccgtctcac
     1141 cgtttcaccg ccctgtcgac cggggctgtt cccgtctcac ccagcgccac gtaggcgcag
     1201 cacgacgctc tgcagcacgg cgagcagctg cgacacgttg agcctgtccc cttgcccgtc
     1261 gggcagcaat gaccccgtcg gggatcacca cctcaggaat gaccaggcgc gagacagcaa
     1321 attcactcat cgccaccccc gaacgtctcg gacaggttca cgccgtttgc acgctgctcc
     1381 agcgcctcgc cagccgcagc aacatcggga tccgaagcaa ccagctcccg ggtgcgcttg
     1441 cgctggaacc gcagtcccag cgcgtacaga gacgcatccg aacggacgaa agcatccgtc
     1501 tggccacccg cagcgagcga tccccggaac ctgccaagcg cccgccgctc agtgaccgat
     1561 cgatcgcccg ggtggagccg cagaacgggc aacagccaga tcagctccag ccgttgcgag
     1621 cgcgtgatcc tgctggacag cggcatcaac acctcgccct gcaggtgacg cggcagctta
     1681 cgcattccag tcaataccac gcgcagccgg tgacagcgtc atgccacccg cctgaacctg
     1741 acgacgcacc tcggcaaacg ccggagcacg cgccgcgtct ggcatcccac gagaggacgt
     1801 caacacgccg tcaagtaaca ccaggtcagc cggtgccaca cccgccgctt tcatctcgtt
     1861 caggaacgcc gcgtcttgga tggactgcgc acacgcacgg tgaaccgctt cggtcggaga
     1921 cgccaccgga gccccgatcg gcgcgaacgt ctgcgctgcg ccgcctgtct ccaccactgt
     1981 ctcgaccacc gacgacagcg gagggaggcc aaagtcatca gcgccggtgg atttggtcag
     2041 cgtcttgcaa cctttaacgt cgttcatgcg cttacggtgg agagacgcga tcgcctcgtc
     2101 ctcctccagc tccccagcgg gctttttgcc cttcttgccg cccttcaggt cgggatccag
     2161 agattgcttt ttgaaagcgc cggccccgtc agattttgcc agcgtcgcct cattgcgaag
     2221 ccggtcacac aggagtttga aagcagacat tacacaccct ccatgccacg gttacggcgc
     2281 aggatttcaa tagcgccctg cgtggcgaaa atttggttaa gacggcggtc aggcgcgatc
     2341 acggtacggt acaggccgcg cccgccgtcg gtttcagcga gcgtcgtcgc ctcgtccatc
     2401 gccagcttga gaagcagctc gatacgctgc acgcgaacgg agtgcgccca gttgaacagc
     2461 gccagcgcag ccgccgcgtc acggcgatcg gcatcctgca gcagggtgtc acgctcccgg
     2521 gccacgtcgg cctcagcagc ctcagcaaca accagctcgg cctcgatgtc cggcagcagg
     2581 aacgacgcaa cacccgcgtc ggggctcacc ggctcgccag cacgcagcgc agccttgccg
     2641 tcggcgatca agccccgcag ccgttcggcc tcggtcttga gcccttccac cactcgagcg
     2701 gcgtccagcc gtcgcgcttc cacgtcggaa gcggcctgtc gcatttcacg cgcagcctga
     2761 cgatcggcga gcgccgagag ctgaatggag gagttcgtgt ctacctgcag ggacgggagg
     2821 tcatacacag ggaacgcatc acccgccaat tcggcaaggg tagagcgtag ccaagcgtcg
     2881 tcggcgctct ctttcttaat ttcggtcatg gtttggttct cagcaagtta tcagcaaagc
     2941 ggcaagaaac gccgctcaga cagcatgccg tcacgacgca gcgcagacat caacgcagag
     3001 ggcgcagggc tctcgcccag cgcggggagc cgcctggagg ccgtcgctaa tcccctacat
     3061 ttcaggacac ccaacagaac gacgaaaaag acactttagc gatagacttt cgatacagtt
     3121 gtggcatgtt gtgtcgaaag agacgtatcg gtcagaggag agaaaaaagt gaacaaaaca
     3181 tacggttacg ttcgcgtatc aaccgacgaa caaaccagca acagccagcg agacgccatc
     3241 atcgcccggt tcccaaccgc ctccatcctt gccgacgacg ggatcagcgg gacggtacca
     3301 ccacgcgagc gccctgccct cggcatcctc ttgaaccagt tgctgcagga gggcgacacc
     3361 ctggtcattt acgcgatcga tcgcatcggc aggaacgcca gggacaccct cgagctggtc
     3421 gaggagctgg agaagcggaa gatcaccctg gtcagcatcc gcgagggcgt cgacctgagc
     3481 agcgccgtcg gacaggcggt actcggcgta atggccagct tcgcccagat ggaacgcaag
     3541 ctcatcctcg aacgccaggc agcaggtatc gaggcagcaa agagccgagg ccagaagttc
     3601 ggacgcccga ccaagatcgg agcgaaggaa gccggggaga ttgtcatgcg ccgcgccatg
     3661 ggagtgccga tcgccaccat agccaaacag ctaaacctaa gccggagcgc gatttaccgc
     3721 catcttgaga aggtcgacgc ggtcgcgccg gagtaagaga gagatcgcgg cggtggtact
     3781 cgctaaagtc accgccgcaa cccaggcccg gggaagtgtg agagccaacc cgggcatcac
     3841 caaccacccg ttagaggttt aatgacatgc agagcataca acagaccacc cctgcccgct
     3901 acccgggcct gagcatcgaa cacggcgaga tccgccagtt tggcaagccg cgtcattgcg
     3961 ccgccaaccg ccgaggccag taccagacta ccctccgcga ccaggcaggg cgataccgca
     4021 ccgtcagcat catcagcctt tacgcatggg aggcgtgacc ataacgaaag ccttacaggt
     4081 gtaagcaaca agcaatagca aaagccttac accattacga accacttaca ggggcgcgaa
     4141 agcgccccca gaaaccagaa tgacaaggag agaacaatgg catttgaaaa cattccccgg
     4201 aggttccaga gatggaaggt aatcatggtt cctggagcga agtcatacga gtcatgggat
     4261 gagatggtta caggcgagaa cttctccgaa gaaaccagga tgaaaacgta caaaaagcac
     4321 cgcaccaagg ctatcgaccg caccttagca aaccaggagt acatccatac catgacggag
     4381 gatgggatca ccgtcaggta ccgcctcgaa cgctggcgct aaccccaacc aggagcgcag
     4441 cgacggctgc gccctccccc ttcaccagaa cgcaccagag ccattctaag cgcctcagcc
     4501 acaaaaacac ccaaccgcac gaccacgcac gcacaaacgc cgtgtacccc ccaaaaaccc
     4561 ccgccatttt cagcgcgtga cgtttcaata cgggagagaa accaaccgga gcaggaacca
     4621 tgaagaaata caccaccgaa cagaaagccc aggcgctcag gctactggag caggacggcg
     4681 caaccagtgc caccgtcgca cgcaccatgg ggatcccacc ccgcaccgtt cgccgatggg
     4741 cgagcgagaa ggccgcagca cccagcaacg tcctcagcat cgaggagatg cggaaacgcg
     4801 ccgccgccgc cgtcgaggcc acaccacagg ccgcgatccg acgccttaaa aaccactttg
     4861 tacagcagca atttgacctg ctacagcgcc acgcaaagga cttgcaagcc ctccgcagcg
     4921 catcgttaca ggccatgctg gagaaggacg ccaccatggt caaagcgatc agcggcctca
     4981 tgaccgccct gctcaaagcg caggaacgcg aacgcctcat ctacgagatc aagccgggca
     5041 ccgaggccga catcatgcgc gagggcatga accgcaaaca acaatgaccg ccatgaccga
     5101 aatgaccgcc atgtgaccgt ttttgtgacc gccaaaaccc gggagcccac ggaatacgcg
     5161 gatccgcacg ttacgcaatt gttaccgctc gcgtgcgcga gaagccccgt tttgatgccc
     5221 ggacgaacca aacgcgcagc tgcagcaacg cattccacac aagccgcaga gggcgcgagg
     5281 gcacgccctc acgctgaacc accaagcgac cacccagcgc gaggcgaaag cctcgtgccc
     5341 tcagcgccac tcaaaccacc gatcgaccaa gacaagcaaa gtgcatcccc gaaggggtgc
     5401 agctgcggcg cgtcaactgc gcaatttttg agcgtgtggg ggtatgaggg tgtaggggta
     5461 acccatgggc atacaggggg tgtgggtgct acttattacc ccttttttta catagtagat
     5521 aaataaaaaa atgtacccca acatacccca acactaaatc cacaagcacc gccattaaag
     5581 ggatttagag cgtgtggggg tagattttct ttgcgatttt acccccacat tttaccccca
     5641 cacgaccaat tacccccaca cgttaccccc acaccgaacc cccacacgat caccccatac
     5701 ccccacacaa aacggaaccc ccacacgggt accccaacat ggcagaggca taaaaaaacc
     5761 cgccgaagcg ggtcattttc agagcgggag ttccccggtc ggttcgtctg gcaatttcca
     5821 gtaaacaaaa gtaccccggt ggttcctgcg atcaacgatc ccctcatcag cgaggcgctt
     5881 caacgcccta ccgacgcgac cagaatggct tgatggggca cgcagcgccg ccgtcacgtc
     5941 cgtcgcggta aacctgccgt catagccagg cttgtcccac gggtactggc cagtagacgc
     6001 attgcccggc aataggagga tgtcgcgcac cggttcatag tacgagccgc gatcgacgaa
     6061 ttgagccgtc tcagctttcg ccgcagcaaa ggtctcgtcg tccaggttga aaccgacgcc
     6121 agagcggtac aggtgacgaa cctcgagcca gaactggatc agctgatcct catccgcccg
     6181 aacagcgcag cctttgacgt tatcccactc ccacccgagc acctcgttaa cgcgagcaat
     6241 gtcgataggg gcgacgaggg taatggtcgg gaagcgggac gccgccgtct cgtccttcag
     6301 gtagtcgcgc ttgttcaccg tggcacccat caccatctgg cgctttttgg cgatcacagc
     6361 cttctcgttt tcaggacgcc actcatccac gttgcgctcc acgaacgcct tgatttcatc
     6421 ggggtcgttt ttcttcatca tgcccgacac ttcacccagc tcgttaaacc agcgcatgac
     6481 cgcagggcgc acgctgtcct tactcgaagg gttgatagac acaccctgcc cgaacgcacc
     6541 aggcagcagg tcgaacagag cgcgaaagaa cgccgtctta aaccagttgt tagcctcgga
     6601 gtagaggcaa ggaacgtact tgttcgacac cgccccctcc tcaacagcgg tgatcgccgc
     6661 aatcagcagt ccacgcagca gagccgagcg cagcgcctcc ttaccaggtg cgacaggcac
     6721 gcagtccagc acggcatcga cgcgaggaga accgtcccac tcgcgggaac cgaggaggcg
     6781 ggcaacaggg tggtaaggag cggaacgggc aacgtagaac atgaaatcgt caacagcctg
     6841 acgtttcagg ccaacctcgg cgcttttgtt cagtatccag gcgcgagcgc catgctcgtc
     6901 cggttcgacc agagagccgc tatcagccca tgagagctca ttcttgaagg tcatgaggtt
     6961 aagctccatc cgggcaccac tgcggtagag caaccagcgg agattgtcgg cgtgattgag
     7021 aacgtcccag cgcccagtgc cagcatcaga gatcagcggc gggacgtacc gcagcgggta
     7081 ctcattgccg aaatttatcg atcgcttttc agccgcctga atgcggaaac cgtcgcgggt
     7141 cgggttaccg gttagcccat cccactcatt gaaagggaca ggaggcagag aaccacccat
     7201 gaacgcaggg cgctcggtct tggcggcagc tttggccttg gcggcggcga tctgcgttac
     7261 ggtggcgaca gcagcgccag aattttcttc atggtttgac aattttacgt cctcaaaatt
     7321 gaacagccaa tctcgaggag ccttacaacg gcgggggata acggggaaaa caccctttgc
     7381 acgcgtcatt atgatcgcgt ataattcatg ctggagtcac acccaacatg agttattatg
     7441 gcgtcttcca cggtgaccgc cgcgaaagct ccaaactcaa aacagcttta agaaaacgac
     7501 ccgtctgccc ggacgggttt tctttttcag ccgcgccatc ataccgttac atgcccccct
     7561 gcaccagtac acaacgatcc aaccacgcga aacgcgatcc cgcaccgatc ttagagccgc
     7621 gcagcctccg cgatttatcc acgggccgga acgttcgaaa ttttgttatc cacagaagtt
     7681 atccacgggc cgagccgcta cccctccctg cacgacaatt gcactacgtc aaattcgacg
     7741 cgcacgacaa aacgcattac actccgcaag acaaacagca cgacaaaacg cacgacacgg
     7801 agaggccagc ccatgcgaga gaaaaccaga accctaaccg ttcgactgcc cgtcagcctg
     7861 atcgagcgca tcgagcagcg agcggagaaa accaaagtaa cccgcacaga tgccgtcata
     7921 gaggcgcttg tcgccggact cggcacgaag ccggaaggga tggacaccaa acttttgacc
     7981 gccatcgagg cgctgcggtc agaggtcagg gcgctggccg gaggcgggaa agatgcacga
     8041 cagtctgcac cacaaacagc acgacaaagc gcaccacaaa acgcacgaca gatagtcccc
     8101 cggggagccg atccccgctt ccaggccgtc agcgatgagg ccgaccagct catcgtcagc
     8161 atgaccgggg agaacgccac cctgcccgcg atcgcccagg cgctgaacgc agccgggcac
     8221 accaccgcca ccgggcagga gtggaatacc agccgcgtcc gggactaccg ccacaagctg
     8281 cgcgagcgcg gcctgatttg acgcagaggg gcgacggctc acgccgccgc cgcatccagc
     8341 cacggcagca tcgccgccct gatcagctcc acgtcggcct cgtccatggt cagcagcacc
     8401 atgccatcca caggaggcaa cccggcgaac gccgcgcagc cggtcacgtt gaacgactcc
     8461 actatcagcg cggtggccac agcctccctt tcgaccgcgc acagccagat gtagttacgc
     8521 tcacgcgacg ccggtagctt cacctgcagc gcacggtttg cacgatcggt aaacgcagcg
     8581 gtacccgccc aggtgtagtc gaacggcggg cgttcgagca ccacagagcc ctcctgcgtc
     8641 tgctggcgcg gcagcgggga gaagttcggc agcgagccgt gagcaaaccc gtcgagcgac
     8701 caacccccca tctcagaagc gatcacctca agggcgcgag ccgtcgcggc agcgcgtaat
     8761 tcagtctttg atttcatgtg ccctccttac gggcgaaaaa aagcccgaca ccaaaacggt
     8821 gccgggcttg agtgtgctgc tggaaaccaa accagctgca ggatagcgtc acgaccgcag
     8881 cggcggtgcg tcgctttaat ccgtctatgc aaaaaagcgt cgatagtgtc tcgaaagtcg
     8941 gtcgcaaaat ccggggcagc ggcagcagga ggtgtgtccc gaaaggcgca ccattcggac
     9001 acgtcaatca acgccgctca agccgggcat gacgaaccgc ataccggagc agctcacgtg
     9061 cccgatcttc cccttcctca accagcacgt cgaaccagtc gaggttatcc cggggagtga
     9121 accgatcgag gccgtcgaac gacacaaccg ccgcgcctgg aattgtcggg accggctctc
     9181 gctcatcgcc ataggccgct ttatgcgcca ggcggtacgc ttcgccgtga tccggtacca
     9241 gcatcacatc aacatctggc cagaaccgac gcagcgacgc cacgcagctg ccgaacccgg
     9301 cgatcgagaa cgcagcgcag accgccacct tgccacggct caggtggtgc agggcgaacg
     9361 ccgtagcgaa cccctcacag acgaacagag gcgagtaggg cgcgtgcggg gagccgtcga
     9421 gcacgtccat gaaccccggc atcgggtaga agccgccctg cttgcgggta cccgccggag
     9481 agaacttcga gccagtgagc tggaaaccct cagccgagca acgcccgcag atggcctcca
     9541 ggccgtcaac atacagctga cgggcggtgt cgaacttgta aaccggaatc aggaggcaat
     9601 cagaccagta agcacgcgga ttgaacgatc gaaacatccc gccaacacca agatctgtgc
     9661 tgagggcggg cagctggacg ccacccgcag ccgccagagc ggtctttttg cgccagtatg
     9721 gggtagacgc aggatcccct cccttcgcca gcagcttgac cgcagcacgc gcagcagcat
     9781 cacccttctt gtcgcgttca gcctttgcct tcgctgtggc ctcacgggac aaccgcagcc
     9841 gttcagcgcg atcgacgtcg gactggacag aagcgcgacc gttgcgccat tcgacgccgg
     9901 agaaattgag gaaagcctga gcggcgaatg tatccaggtc gccttttaaa aacgacctgt
     9961 actgctggcg atggcgattc tcgcctcgcc catgtcgagt ggaccaggcc tcgatagctg
    10021 ccagcccgtc ctcgccgcag agagacgccc agcaaccaag agccaggcag agacgcaccc
    10081 attcgtctcg atcagccgga gcacccgcag ccgatacggc acgccagaac aggcccggat
    10141 cctctttcag atgggtagtg tcgaaatcaa cgaacgccat cagcagccct ccccggacga
    10201 cgctggtcgg caacgaagcg tttgatctcg ggattgccag ccagccagta atgcccgcca
    10261 cggagggagc gccctgccgg agtcggcacg atcccccgcg aacttagccg cgagagcgtg
    10321 cgcagcgaca cgttcagcag cgcagcgagg tcagcggtat agattcgttt tttcattggg
    10381 agtaccagat aggtgccccc ctcagcttaa cgggcatcgg ggggcgggag tgacgattat
    10441 cgataaagcc gcccttgagg acttaccgca tagcggcagg acagcagccc ttctcgctta
    10501 ctcgcacctg ccaaggacga agcgcgcatc ctcggttaca accccgcctc gttggggagg
    10561 agggaaagcg gcaccgggcg atccggtttc atgtttccca actgctcgtc aggaatcagt
    10621 aagacaagcg taacacaatt aacgccaaag tggcgttttt tgtgagatac taaaaaagac
    10681 gcacagccag tgctggcgct gtctggcgac catttcgacg cagggggagc agtgaacgac
    10741 gataacgaca cgaacgaatc gacgcagccg aacccggcag cacttgaacg cgtaagcgcg
    10801 actgaaaaat tccccgagca gctccccggc gttttgttcg tcacctgccc cggcgggtgc
    10861 tccctccggg tcgaggcatg ccgacagatt tatgagcaga gccacctcga tcgcacgatt
    10921 gaaaaccccc acctcgagtg ccaccggtgc cccactggcc tcgcgctttg ggtcggtacc
    10981 gaacagcccc ctgacgcacc actgaccacc ccggcagaca cccgtaagct ctgcccccgt
    11041 tgcagccgga aagacaaccg cctgatcgcc cccaataagc ccggttcgag cctgtgcgtc
    11101 tcctgctgga accgcgaacg cgaagccacc atcggagcca acgcacgcgg gagagtcaac
    11161 cgcaccccac cgttcgtctc gccgttcctg atcggcatcg gcgatggctg gcaggtctgg
    11221 tacggcatcc accagggcga ggcgctcgtc cgcgccatgc gagccaaccc gggcgcacgg
    11281 ttccacgatc gccgaccagg cccggtcggg caaacacgcc gcgacggttt cgtgtactac
    11341 tgccacgaac accgcgagcc cctgcgctgg aacggtggcg acgggcacgc cggagaggtg
    11401 gagcttgctt gtcccaggtg ccagccatgg gcggcacccc tcagcgtcgc ccccacgttt
    11461 cagacggtca gcttccagac accgcatgac gccgcgcacc agtaccggaa cgccaccctg
    11521 ccagacaacg cggt
//
