LOCUS       NZ_CP060945             2101 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC11 plasmid pEC11-8, complete sequence.
ACCESSION   NZ_CP060945
VERSION     NZ_CP060945.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904655
            Assembly: GCF_022558905.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558905.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:22:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,840
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 72 of 241
            Pseudo Genes (incomplete)         :: 159 of 241
            Pseudo Genes (internal stop)      :: 52 of 241
            Pseudo Genes (multiple problems)  :: 39 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060945.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC11"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC11-8"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-24"
                     /collected_by="Long Sun"
     gene            join(1819..2101,1..683)
                     /locus_tag="H9198_RS24685"
                     /old_locus_tag="H9198_24445"
     CDS             join(1819..2101,1..683)
                     /locus_tag="H9198_RS24685"
                     /old_locus_tag="H9198_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            687..953
                     /locus_tag="H9198_RS24690"
                     /old_locus_tag="H9198_24450"
     CDS             687..953
                     /locus_tag="H9198_RS24690"
                     /old_locus_tag="H9198_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          112..343
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atcaggctct accgaaaatc gttgtggatt acccgtcttc ccgatggttg tttctgacgt
       61 taactgtcag gaactgcgag ataggtgaac ttggaacagt ccttacagca atgaatgcgg
      121 cgtttaagcg aatggaaaag cgaaaggagc tatcacctgt tcaggggtgg atcagggcta
      181 cggaggtgac gcgaggtaag gatggcagcg cacatccgca ttttcactgt ctgctgatgg
      241 tgcaaccttc ttggtttaaa gggaagaact acgttaagca cgaacgttgg gtagaactct
      301 ggcgcgattg cttgcgggtg aactatgagc cgaatatcga tattcgggca gtaaaaacta
      361 agacaggtga ggttgtggcc aacgttgccg agcaactgca aagcgcggtt gctgaaacgc
      421 tgaaatactc cgttaaaccg gaagatatgg caaacgatcc tgagtggttt cttgagctga
      481 cgcggcagct tcacaagcgc cgttttatct cgaccggtgg ggcgctaaaa aacgtcctcc
      541 agttggatcg agaaaccaat gaggatcttg tcattgccga cgatgtaggg gatggcactg
      601 atgacgggaa gcggacggcg tttgtctggg attcaggtaa acggcgttac aaacgcgccc
      661 ctgagaagga taaatcggat taacgtatga atattaatat tgaatacctg aatggaaata
      721 agactattgg tttatttttt ttaagaagtg aagcggtgat tcctgacagg tttaaaaacc
      781 ttattttgct tattgatgga ttaagttttg gcacatttgg ttttcatccg cacgaaggtt
      841 ttgaggatga attaatttta tatattcaga aaacaaacga gagggtaaaa actctttttg
      901 tgaaaataga tttaaacggt atcaagtttg gatttttaag aacgcattct tagttctgga
      961 aaagagccag cggcagactg aggtgatagg tacgagattg catgcaatct ctagtgctct
     1021 gtctatcctg cattatcctc agcattatcc tcagcattat cctcagcctt gccaactcga
     1081 caccaatgca ggatagacaa tccgatgtca aatgttaaca ctctgcgagt ggtacatttt
     1141 ccccggatta tcgtcctgag cctgccgctg gctctctttc taccgcctcg ctttgctcgt
     1201 tgctcaacgc ctcacagaca cggattaaaa tccgcatccg ttcaccgttt tttaaagtcc
     1261 gttaaaagca tgatgccatc tccgagagtt aatctcgtca aatgctaaat cgtgggggtc
     1321 ccctttgggg ttccgattta gtgattgacg acaccaccga ttaaaaaact tatgcggggt
     1381 ggatggtttc acgaagtgag gccatccacc tgtaagacag ggttttgttt ttattccctg
     1441 ttttggtgat cgggtgtgtg gaaaaggttg gggtaagccg ttcgggggtg cttgttttgg
     1501 ggggttaaaa ttgtggttat tttttgcgca attctcgcgc gtgatccttg tatttatact
     1561 taagggataa atggcggata tgaaatagtg gtttagccca gtaatgacga ggctttgagt
     1621 gggttttgac aggtcaaaga aaatggagca gaattgaggc gtttttaatc ggcgttgggg
     1681 agtgcgtcaa cactccccaa catttcgaat gtgtcacctc agcggcaaac tctggtgaca
     1741 tgtactggct cgcaatgcac aggtacgtga tgaatatacc acatcaaatc acagcctgcc
     1801 cagatcggag caggcttaat gtcagaagat aaattccttt cggactacag cccccgtgat
     1861 gcagtttggg atacccagcg cacgcttacc gattctgtcg ggggtatcta ccagactgct
     1921 gctgaattcg agcgctatgc actccgtatg gcctcctgta gcggtttgtt acgttttggt
     1981 tggtctacca tcatggaaac cggagaaacg cgcctacggc ttcgtagtgc gcaattttgc
     2041 cgtgtccgtc attgccctgt ctgccagtgg agaagaaccc tcatgtggca agcccgtttt
     2101 t
//
