LOCUS       NZ_CP060944             2261 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC11 plasmid pEC11-7, complete sequence.
ACCESSION   NZ_CP060944
VERSION     NZ_CP060944.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904655
            Assembly: GCF_022558905.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558905.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:22:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,840
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 72 of 241
            Pseudo Genes (incomplete)         :: 159 of 241
            Pseudo Genes (internal stop)      :: 52 of 241
            Pseudo Genes (multiple problems)  :: 39 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060944.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC11"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC11-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-24"
                     /collected_by="Long Sun"
     gene            complement(join(2120..2261,1..854))
                     /locus_tag="H9198_RS24675"
                     /old_locus_tag="H9198_24435"
     CDS             complement(join(2120..2261,1..854))
                     /locus_tag="H9198_RS24675"
                     /old_locus_tag="H9198_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_070749935.1"
                     /translation="MQNPKLKNLTDYSPDDKPWDAHKSQADDVGGIYLRAAEFESYAAR
                     MRDCGGILRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDGRWMFLTLTVRNCAIEDLGETLTAMNAAFQRMKVRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPSMLSGRDYVKQYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     gene            complement(1696..1881)
                     /locus_tag="H9198_RS24680"
                     /old_locus_tag="H9198_24440"
     CDS             complement(1696..1881)
                     /locus_tag="H9198_RS24680"
                     /old_locus_tag="H9198_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_000166333.1"
                     /translation="MTKQQRIDGFRKAEASLKLEGMDPSGTPFYESVKARIISGEITYE
                     QGKAEILSHYKARAEG"
                     /name="antitoxin VbhA family protein"
ORIGIN
        1 tcctgctcca gtttcagaac gtccttaagc gcgccacctg tcgcaacaaa gcggcgttta
       61 tgcgtctgcc gggtcagctc taagaaccag tccgggtcag caaccatatc agccggcttt
      121 gtcgaatatt tcagggtttc agcaacggca ccgcgcacaa gttcggcagt tgcacatgcc
      181 agcgtctggc catctttagg cttgcggggt ttcactgccc gaacatcaac attcggatcg
      241 taatcaacac gcaggcagtc gcgccagagt tcaacccagc gatattgttt tacgtaatca
      301 cgaccggaaa gcatcgaggg aggaaccatc atcagggtgt gaaagtgggg gtgtgcagag
      361 ccatcacgac cgcgggtaac ttctgtggtg cgaatccatc cctgtacgga tttaaactcc
      421 ttgcgaacct tcatgcgctg gaatgcggca ttcatcgccg tgagggtttc tccgagatct
      481 tcaatggcgc agttacgaac ggtgagcgtc agaaacatcc atcggccatc agggtaatca
      541 gcgacgattt tcggcagaga ctgataaaaa cgggcttgcc acatcaggga gcgcctccac
      601 tggcagacag ggcagtgacg aacgcggcag aaatgcgcct cacggagccg taaacgcgtt
      661 tctccggtgt ctttaagggt agaccagcca aaacgcagga taccgccaca atcgcgcata
      721 cgggctgcat acgactcaaa ttcagcggca cgcagataga taccgccaac atcatcagct
      781 tgtgacttgt gagcatccca cggcttatcg tcggggctgt aatcagtaag gttctttagc
      841 ttaggattct gcatggcacc tgctccggta tgggcaggca cagaaagggt gtgttatcat
      901 ttccgtatca cctgtagaga gagtcttaaa ccgaatttgt ccaggtgatc gcgcccctgg
      961 agtgctggta acactcgcag gggttattca tatcatcgtg tcagtaagta aggtcttggg
     1021 aaccacccca agtaatcaaa aattagttcg cggacacgat cacctgtcag aaatcatact
     1081 gccaaaacct tttccgagca aattggctct caggccttgc ggcacaaggg ctggaagcga
     1141 atttaaatcc tagagttatc ccttaagtat aaatacaagg attccgaccc tttcagggcc
     1201 gaaaaaccaa ccccaaaaag tgaattcaaa cactcaaaca tgaaagcact caaacactcg
     1261 caactgagca tcagaatgag acaaacgtgc agtattacat tcactgcacg cggctgtttc
     1321 cgttgcaaat cgcaacaaga gtccactatt aaacagagtg gaccccgctt ccgaacggcg
     1381 tatgcctcct cgcagagctg cgggggcaat ccccgttcct catcgcggcc gcacgacatg
     1441 acccctcaca ctcttcatgg cagatttaaa cgaacaaggc acaccgagca aaacgaggat
     1501 acccccacgg catcacctgt agggcagggc ttgcgcacct accccacagg caatgccttg
     1561 aagggggtaa aagcggcttt acagggcgat ggctcactac gtgaaccatc acccatcaca
     1621 agggtatgag gggtgcagtc gtacaaccac taaatcggaa ccctaaaggg atcccccgat
     1681 ttagaagttg acgattcaac cttctgcgcg ggctttgtag tgggaaagaa tctcagcttt
     1741 gccctgttcg taggtgattt caccggagat aattcgggct tttacagact cataaaaggg
     1801 agttccagaa ggatccatcc cctccagctt tagcgatgct tccgccttac gaaacccgtc
     1861 gatcctttgc tgttttgtca ttgattttcc tttgagcagg atgcgaataa ggcaggcgta
     1921 aagtgggccc actttacata tcctgcccga attctgccgt tggttatgca tgtatgcgtg
     1981 tatgcatgca gagtggaaat ctcagggaaa cccgaaggaa tgccaaagga aatctgtgta
     2041 ccggacaaga tgtgtttttg gagtaccgcc acacgcagcg gcatccaaaa acaaccaggt
     2101 cagggcaagc cccgacaccc taatcaggat ctgatttaaa cttatcgcgc tcaggcaatc
     2161 ggacgtattt tcttggcgtc gaatcccagc caaaggcgag gcgtgaacca tcatcttcgc
     2221 catcggaaat atcatccccg atcaccatgt cctgatccgt t
//
