LOCUS       NZ_CP060942            21115 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC11 plasmid pEC11-5, complete sequence.
ACCESSION   NZ_CP060942
VERSION     NZ_CP060942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904655
            Assembly: GCF_022558905.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21115)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21115)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558905.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:22:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,840
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 72 of 241
            Pseudo Genes (incomplete)         :: 159 of 241
            Pseudo Genes (internal stop)      :: 52 of 241
            Pseudo Genes (multiple problems)  :: 39 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060942.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC11"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC11-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-07-24"
                     /collected_by="Long Sun"
     gene            join(21058..21115,1..941)
                     /gene="fecC"
                     /locus_tag="H9198_RS24540"
                     /old_locus_tag="H9198_24305"
     CDS             join(21058..21115,1..941)
                     /gene="fecC"
                     /locus_tag="H9198_RS24540"
                     /old_locus_tag="H9198_24305"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="WP_000125175.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALLP
                     GHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAALA
                     MALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALSAFC
                     MGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQLNLLN
                     LSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAGFDQRN
                     VLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
                     /name="fecC"
     gene            938..1894
                     /gene="fecD"
                     /locus_tag="H9198_RS24545"
                     /old_locus_tag="H9198_24310"
     CDS             938..1894
                     /gene="fecD"
                     /locus_tag="H9198_RS24545"
                     /old_locus_tag="H9198_24310"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease subunit
                     FecD"
                     /protein_id="WP_000684856.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHYY
                     VLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLLMP
                     SLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSRPQD
                     VNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLGVSVP
                     HTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTGALLLV
                     VADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
                     /name="fecD"
     gene            1895..2662
                     /gene="fecE"
                     /locus_tag="H9198_RS24550"
                     /old_locus_tag="H9198_24315"
     CDS             1895..2662
                     /gene="fecE"
                     /locus_tag="H9198_RS24550"
                     /old_locus_tag="H9198_24315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="WP_000175457.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTLL
                     NCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRNPW
                     LSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVVLLD
                     EPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMAQGTP
                     EEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
                     /name="fecE"
     gene            complement(3220..3477)
                     /locus_tag="H9198_RS24555"
                     /old_locus_tag="H9198_24320"
     CDS             complement(3220..3477)
                     /locus_tag="H9198_RS24555"
                     /old_locus_tag="H9198_24320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000177057.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFPL
                     AKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
                     /name="hypothetical protein"
     gene            complement(3560..4756)
                     /locus_tag="H9198_RS24560"
                     /old_locus_tag="H9198_24325"
                     /pseudo=""
     CDS             complement(3560..4756)
                     /locus_tag="H9198_RS24560"
                     /old_locus_tag="H9198_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            4942..5784
                     /locus_tag="H9198_RS24565"
                     /old_locus_tag="H9198_24330"
     CDS             4942..5784
                     /locus_tag="H9198_RS24565"
                     /old_locus_tag="H9198_24330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_160371899.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLAE
                     NNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLITSF
                     IGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTHTRW
                     DKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELGFHYL
                     YTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPRLPDD"
                     /name="polysaccharide deacetylase family protein"
     gene            5787..6875
                     /gene="capU"
                     /locus_tag="H9198_RS24570"
                     /old_locus_tag="H9198_24335"
     ARG             5788..6872
                     /name="capU"
                     /gene="capU"
                     /gene_name="putative hexosyltransferase CapU"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="99.72"
                     /coverage="100.0"
     gene            6880..7830
                     /gene="virK"
                     /locus_tag="H9198_RS24575"
                     /old_locus_tag="H9198_24340"
     CDS             6880..7830
                     /gene="virK"
                     /locus_tag="H9198_RS24575"
                     /old_locus_tag="H9198_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="WP_001333383.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINPV
                     VSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNLPE
                     KYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTFSVI
                     LTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECLA
                     VSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKK
                     RSEYRKRYALLDYIHETTISSLDAYPVHSEHQNLN"
                     /name="virK"
     gene            7895..8839
                     /gene="lpxM"
                     /locus_tag="H9198_RS24580"
                     /old_locus_tag="H9198_24345"
                     /gene_synonym="msbB"
     CDS             7895..8839
                     /gene="lpxM"
                     /locus_tag="H9198_RS24580"
                     /old_locus_tag="H9198_24345"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species.; LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="WP_001333384.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPV
                     LAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFITALQSIVMMAELAIR
                     GPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQR
                     NPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFA
                     TYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMN
                     KTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
                     /name="lpxM"
     gene            9020..10159
                     /locus_tag="H9198_RS24585"
                     /old_locus_tag="H9198_24350"
     CDS             9020..10159
                     /locus_tag="H9198_RS24585"
                     /old_locus_tag="H9198_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_000088357.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQWV
                     TAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILNCTGVAEKYALIEQWRQQFP
                     IEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAE
                     NGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVA
                     DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHH
                     SDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDE
                     AISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
                     /name="IS3 family transposase"
     gene            10313..12310
                     /gene="betT"
                     /locus_tag="H9198_RS24590"
                     /old_locus_tag="H9198_24355"
     CDS             10313..12310
                     /gene="betT"
                     /locus_tag="H9198_RS24590"
                     /old_locus_tag="H9198_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="WP_032293733.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKNF
                     KLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAMLFS
                     AGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVALIL
                     AFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQVNAG
                     LNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLG
                     PTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWSPFVGMF
                     IARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLAKTVQQDVALA
                     LFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWASL
                     MGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKRESLSMATIA
                     PTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQGTISHISDAVD
                     DRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYRAEVYLKEGGQN
                     YDVMGWNQEQLINDILDQYEKHLHFLHLVR"
                     /name="betT"
     gene            12373..13650
                     /locus_tag="H9198_RS24595"
                     /old_locus_tag="H9198_24360"
     CDS             12373..13650
                     /locus_tag="H9198_RS24595"
                     /old_locus_tag="H9198_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="WP_000625669.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPESV
                     SVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQNV
                     LATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSLGRV
                     GETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDMVKLS
                     KLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFAQDPRF
                     CLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVHVAPLQN
                     NDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETLEHATRAD
                     HIDSDRYLIKSTYYNLFSGEYSNKKT"
                     /name="DUF2254 domain-containing protein"
     gene            complement(13930..15092)
                     /locus_tag="H9198_RS24600"
                     /old_locus_tag="H9198_24365"
     CDS             complement(join(13930..14831,14831..15092))
                     /locus_tag="H9198_RS24600"
                     /old_locus_tag="H9198_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="WP_085949416.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQ
                     LYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKYVF
                     IEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRY
                     GAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDF
                     YTSGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALW
                     RRTRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVEC
                     IHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
                     /name="IS3-like element IS3 family transposase"
     gene            15170..15232
                     /locus_tag="H9198_RS24605"
                     /old_locus_tag="H9198_24370"
     CDS             15170..15232
                     /locus_tag="H9198_RS24605"
                     /old_locus_tag="H9198_24370"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113705808.1"
                     /translation="MPYIGLTSNYLYDSFEFGRR"
                     /name="hypothetical protein"
     gene            complement(15243..15940)
                     /locus_tag="H9198_RS24610"
                     /old_locus_tag="H9198_24375"
                     /pseudo=""
     CDS             complement(15243..15940)
                     /locus_tag="H9198_RS24610"
                     /old_locus_tag="H9198_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(16027..16095)
                     /gene="ykiE"
                     /locus_tag="H9198_RS24615"
     CDS             complement(16027..16095)
                     /gene="ykiE"
                     /locus_tag="H9198_RS24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="WP_211180522.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
                     /name="ykiE"
     gene            16232..16753
                     /gene="fecI"
                     /locus_tag="H9198_RS24620"
                     /old_locus_tag="H9198_24380"
     CDS             16232..16753
                     /gene="fecI"
                     /locus_tag="H9198_RS24620"
                     /old_locus_tag="H9198_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="WP_001283626.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDT
                     FLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPEER
                     ESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKAVEH
                     CLLFRLEYGL"
                     /name="fecI"
     gene            16750..17703
                     /gene="fecR"
                     /locus_tag="H9198_RS24625"
                     /old_locus_tag="H9198_24385"
     CDS             16750..17703
                     /gene="fecR"
                     /locus_tag="H9198_RS24625"
                     /old_locus_tag="H9198_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="WP_001068915.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQD
                     NQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSETS
                     EGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTAKDA
                     LQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNAGESL
                     QFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVAGLRLS
                     GTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
                     /name="fecR"
     gene            17790..20114
                     /gene="fecA"
                     /locus_tag="H9198_RS24630"
                     /old_locus_tag="H9198_24390"
     CDS             17790..20114
                     /gene="fecA"
                     /locus_tag="H9198_RS24630"
                     /old_locus_tag="H9198_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="WP_000188246.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLDK
                     ALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSWTL
                     EPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRIPGV
                     SAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPISLGNM
                     DAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPKETHNL
                     MVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLLQYYDGE
                     ADMPGGLSRADYDADRWQSTRPYDHFWGRRKLASLGYQFQPDSQHKFNIQGFYTQTLRS
                     GYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHEMRYYTATS
                     SGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQNNAITGTHE
                     EVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVEPEKARTWELG
                     TRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQARYDLGTLTPTLD
                     NVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTFNLNSDFQSSQFA
                     DNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKNIFDQDYFIRSYDD
                     NNKGIYAGQPRTLYMQGSLKF"
                     /name="fecA"
     gene            20159..21061
                     /gene="fecB"
                     /locus_tag="H9198_RS24635"
                     /old_locus_tag="H9198_24395"
     CDS             20159..21061
                     /gene="fecB"
                     /locus_tag="H9198_RS24635"
                     /old_locus_tag="H9198_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="WP_000879153.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLEL
                     SFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDLII
                     ADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQHKER
                     MAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPSIGLE
                     QLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRGIFAAE
                     RIAADTVKIFHHQPLTVVK"
                     /name="fecB"
ORIGIN
        1 ttattttctg gctgagtctg ttttgctact cggccattcc tgtttccgga gcagatgcaa
       61 cccgcgccct gctgcctgga cacacgccaa cgctaccaga agcgctggtg caaaaccttc
      121 gtttgccacg aagcctggtc gccgttctga tcggcgcaag cctggcgctc gcgggcacgc
      181 tgctgcaaac cctgacccac aacccaatgg cctctccttc actgctcggc attaacagcg
      241 gcgcggcgct ggctatggcg cttaccagcg cgctgagtcc gacgccgatt gcaggctatt
      301 ctctgtcgtt catcgcggca tgcgggggcg gcgtgagctg gctgctggtc atgaccgcag
      361 gaggcggatt tcgtcatacc catgacagaa acaaactgat cctcgcgggt atcgcgctgt
      421 cggccttttg tatgggcctt acccgcatca ccctgctgct ggccgaagat catgcttacg
      481 gcatctttta ctggctggca ggcggagtgt cccacgcccg ctggcaggat gtctggcagc
      541 tcttgccggt ggtggtcact gcagtccctg tcgtgttact gctggcgaat caactgaacc
      601 tgctcaacct cagcgacagc accgcccata cgctgggagt gaacctgacg aggctacgtt
      661 tggtcatcaa tatgttagtg ctgcttctgg ttggtgcgtg cgtcagcgtg gcaggtccgg
      721 tggcgtttat cggtctgctg gtgccacatc tggcgcgctt ctgggcaggc ttcgatcagc
      781 gcaacgtact gccagtgagc atgctgctgg gggccacgct gatgttgctg gcagatgtac
      841 tcgcacgcgc gctggccttc cccggagatc tgcccgcagg cgcagtgctg gcgctgattg
      901 gcagcccttg ctttgtctgg cttgtgagga ggcgaggatg aaaattgcgc tggttatttt
      961 catcaccctt gccctggcgg gctgtgcgct gttatcactc catatgggag tgatccccgt
     1021 gccgtggcgc gcgctgctga ccgactggca ggccggacgc gagcattatt atgtattgat
     1081 ggagtaccga ctgccgcgct tgctgctggc actgtttgtc ggtgcagccc tcgccgtggc
     1141 gggcgtgctg atacagggga ttgtgcgcaa ccctctggca tcaccggata ttctcggcgt
     1201 taaccatgcc gccagcctgg cctctgtggg ggctctactt cttatgccgt cactgcccgt
     1261 gatggtgctg ccgctgctgg cctttgcggg cggcatggcg gggttgatat tactgaagat
     1321 gctggcaaag acccaccagc cgatgaagct ggcgctcacc ggcgtggcgc tttctgcatg
     1381 ctgggccagc ctgacggatt atctgatgct ctcgcgcccg caggatgtga acaacgccct
     1441 gctgtggctg accggcagct tatggggccg tgactggagc tttgtgaaga ttgccatccc
     1501 gctgatgatt ttatttctgc cgctgagcct gagtttttgc cgcgatctcg acctccttgc
     1561 actcggcgat gcgcgcgcca ccacgctcgg tgtgtcggtg ccccataccc gattctgggc
     1621 tttgttacta gctgtcgcca tgacatctac cggcgtggcc gcctgcggcc cgattagctt
     1681 tattggtctc gtggtgccgc atatgatgcg tagcatcacc ggtggacgtc accgcagact
     1741 gctgcctgtt tcagccctga caggtgcgtt gctgttggtg gttgccgatc tgctggcgag
     1801 aattattcat cccccactgg agctcccggt tggcgtgctg accgccatta tcggtgcgcc
     1861 gtggtttgtc tggttgcttg tgagaatgcg ataaatgact ttacgaactg aaaatctgac
     1921 ggtcagttac gggacagaca aggtacttaa cgacgtttca ctctcactgc caacggggaa
     1981 gatcaccgcc ctgatcggcc ctaacggttg cgggaaatcg acgctgttaa actgtttttc
     2041 gcggctttta atgccgcagt ctggcaccgt atttctcggc gataatccca taaatatgct
     2101 ctcatcacgc cagttggccc gcaggctttc gctgctgcct cagcaccatt taacgccaga
     2161 ggggatcaca gtccaggagc tggtttcgta tggtcgtaat ccctggctgt cactctgggg
     2221 gcgtctctcc gctgaagaca atgcacgagt taatgtcgcc atgaaccaga cccggatcaa
     2281 tcatcttgcc gttcgtcggt taaccgagct ttccggcggt cagcgccagc gcgcatttct
     2341 ggcgatggtc ctggcccaga atacgcccgt tgtattactt gatgagccaa ccacctatct
     2401 tgatatcaat caccaggtgg acctgatgcg gttgatgggc gaactccgga ctcaggggaa
     2461 aacggtggtc gctgtgctgc acgaccttaa tcaggctagc cggtactgcg atcaactggt
     2521 ggtaatggca aacggacatg ttatggcgca aggcacacca gaagaggtga tgaccccagg
     2581 attgctgaga acagtattca gcgtggaagc ggaaatacac cccgagccgg tatctggcag
     2641 gccgatgtgc ctaatgaggt agattgcaca ggccgtaaga accaaaccac gactgaatga
     2701 aactggactg gcgccagcaa gcctgttcag actggggctg aacttttccg gactctgaaa
     2761 gattaccaat actcatcgtc catccgcttg ctttaggctg acaggttcat aatcaacgca
     2821 aaccagagct gtacaggctt ggacgcggct ttcaaaccag tcgtgatcac ggcaatcaat
     2881 tttgaactct gcttaacgga catttctgta taacccttac ggcaacgaaa aacgcgaagt
     2941 taaaatttta gaaacccaaa aacgtgacat gactaagttt agatttcagg gggggagatc
     3001 aaaaaatttc gctctgtgcc agagcggaca ttcacggagc tggttcatta ccaatgaggt
     3061 tgggcttttg aggataaatc aatgatcaga cgccaacgta aatcaaaagc aaccctggaa
     3121 cggaattgct aatccagttt ctgaccatcg atttttctaa aaagtgtgcg tttgctacta
     3181 cttaggtagg tgcagctttc ttaatcaccg gcagccacgt tatacaggcc agttgatgga
     3241 tcgattgtta tcaatgatat cttcatgagt cggtgtctca cccagcttac cgaagctggc
     3301 ataaagtgaa ggcagacggg cccgtccttc tccctttttc gccagaggga aagcgcgaag
     3361 catggtggca gcctcccagt cacaacaata ggatggtgtg cacggctgct gacgccatga
     3421 ttcagcgata gagccggaaa atacggggtc aaagccggta tcattaacca gagtcatcgt
     3481 gacctgtact gcggctggat gatctcctgc tactgcaact gctaggcgac cgcggctccc
     3541 ttcaggtagt ctgttgtggt caactaaaac tggcctccgc gttagagttt ttccagtatc
     3601 ggttttctga ttcgtttggt ggtaacccac cattatattc gtgcggtctt agtgcgctgt
     3661 aatatccaac gatatagtcc gttattgcgt gagctgcatc gctgaagctt acatagcccg
     3721 tcgctggcac ccattagttc ttcagactcc tgaagaagcg ctccattggg ctgttatccc
     3781 agcagtttcc acgccggctc atactctgtc tgatctggta tcgccacagt aactgccgga
     3841 actgcctgct cgtataatga ctgccttgat cgcttggaac atcaccccga cgggcttacc
     3901 acgggtttcc catgccattt ccagtgcttt catggtaagc ctgctgtccg gcgagaacga
     3961 gatggcccag cccactggtt ttcttgcgaa caggtcgaga acaacggcga ggtacgccca
     4021 gcgcttaccc gtccagatat aggtcacatc accgcaccac acctgatttg gttccgttac
     4081 ggcgaactgt cgctcaagat gattcgggat agcaacgtgc tcatgaccgc cacgcttata
     4141 ccggtgagtc ggctgctggc aactgaccag ccccagctct ttcatgagtc tgccagcaag
     4201 ccagcgcccc atctggtagc ctctctgggt tgccattgtg gcgatgcttc ttgctccggc
     4261 agagccgtgg ctgatgccat gcagttcaag tacctggctg cgtaatacag cccgtctgcc
     4321 gtctggtttt tcaggacggt ttttccagta tttgtagctg ctgcgatgga ccccgaacac
     4381 atggcagaga gtggccacag gataacgcgc cctgagtttc ccgattatcg aaaactgttc
     4441 agggagtctg acatcaagag cgcggtagcc tttttaatat ttcattttcc atttcaatac
     4501 gttgtagctt tttcctgagc tcacggattt caatttgttc cggggtaatg ggggaggctt
     4561 ttggtgtttt tccctgccgc tcatcacgta attgtttcac ccatcgcgtc attgtggaaa
     4621 ggccgacatc catagcgctg gctgcatctg ccacggtgta tttctggtca acgaccagtt
     4681 gagcggattc gcgtttaaac tctgcgctga aatttctttt ttcattatgg cacctgtgtt
     4741 gttctgaggt gagcatatca cctctgttca ggtagctaaa tttagtgtgc cacctcaagg
     4801 ttcttagtga gccgttttta aaagatatga taatatatta ttgtttataa attgttgatg
     4861 tttgatttag tagtatataa agttcaccac ctttattagg ctttcctttt atctctactt
     4921 actgggtgcc agaaacttta aattttaaac ggagggggca ttttgtttaa agcaaatcat
     4981 gttccggtat taatgtatca tcatgtcagc cattgcccag gactggttac actttctccc
     5041 gagacattcc ggaaacaaat gaaatggttg gctgaaaaca actggaaaac actgtcatca
     5101 gatgaactgg agttttttta tcggggcgga aaacttcccc gtaaaagcgt tatgttgaca
     5161 tttgatgatg gataccttga caactggttt caggtatatc ctttattaaa cgagtttaat
     5221 ctcaaggcac atattttttt aattaccagt tttattggaa atggtccggt tcgtcactct
     5281 cctggaaagg aatattctca tcgcgattgc gaacatcaaa tagcaaccgg gaatgctgat
     5341 aatgttatgc ttcgctggtc tgaagtgaac gaaatgttgc aatctggtct ggtcgaattt
     5401 catgttcata cacatactca tacacgttgg gataaaaagt tcacttcacg agaagaacag
     5461 tgtaaacatc ttcggcagga cttattgagc gggagagaat atttaaaaaa aatgacagga
     5521 aaatgcagta aacatttgtg ttggccggaa ggttattata acaaggatta tattcagatc
     5581 gctgaagaac ttggattcca ttatttatat acaacagaaa gaagaatgaa tgctcctgct
     5641 aaaggaacta cacggatagg aagaataagc accaaggaac gggaaaattg cgtatggctg
     5701 aaaagacgac ttttttatta tacaacaccg tttttttcat cgttgctggc ccttcataag
     5761 gggccaagat taccggatga ctaataatga atatactatt tacggaatca tcaccgaata
     5821 taggtggtca ggaattacag gctgttgctc aaatgaaggc cctgaagaaa atggggcatt
     5881 cagttctgct tgtctgcagg gagaacagca aaattgcttt tgaagccagt aagtttggaa
     5941 ttgatatcac attcgcgtta tttcgaaaca gtcttcacat tcctactgta tggagattac
     6001 tcggaatagt tcatagtttt cagcccgatg caatcgtttg tcacagtgga catgatagta
     6061 atattgttgg tttggtacgg ttttttacct ggaaacatcc attcagaatt atcaggcaaa
     6121 agacatattt gacacgaaaa acaaaatttt tttcaataaa tcatttttgc gatgaggtga
     6181 ttgttcccgg aacaaatatg aagacacatc tggagcagga aggatgtcga actcgggtta
     6241 ctgttgtgcc tcctggcttt gatttccagg aattatacgt tgattcccga aacagtttgc
     6301 ctccgagtgt tctttcctgg ctggcgtccc gaagaggatg ccccgtaatt gctcaggtag
     6361 gaatgttgcg cccggaaaaa gggcacgaat ttatgttgaa tttactgttc catttaaaaa
     6421 tgaatggacg acagttctgt tggttgattg tggggtctgg ttcacctgaa ctgcgggaac
     6481 atttacagta tcagattgac agtatgggca tgcatgatga tgtttttatt gctgacaatg
     6541 tttttcctgc cgcccccgta tatcgggttg ccagtctggt ggttctgcct tcagaaaacg
     6601 aatcttttgg tatggtgctg gcagaagcat cggcattttc tgtgcctgta gtggccactc
     6661 agattggtgg aatccctgag gttattcaga acaaccagac cgggacattg ttaccagcaa
     6721 gtaataagca cgcatggatg tgcgccctga atgatttttt taatgaccct gggcgttttt
     6781 atcagatggc tcgcctggca aaacaggata tagaagagcg gtttgatatt aataaaactg
     6841 cgttaaaaat actcacatta gcgaagcaaa agtaacaata tgttttctat aagtaactta
     6901 tcatttatcg gtttccttaa aaggattatt ttttcctcag attcactacc ggggaagtgg
     6961 gaacacagaa aatttcggtt catgtacatt ttgcgatgtt ctataaatcc ggttgtcagt
     7021 attcgatatt attatgaact gcgttccttg ccgtgcattg aggatattct ggcaatacac
     7081 cccacgttgc cagcaagaat tcatcgccct tatttgcata aaggggggcg cgcctggacc
     7141 cgtggacaat atattcttga gcattatcgc tttgtgcaaa atttgccaga aaaatattct
     7201 aaatttttgt ttccacaaaa atcggtatca ttagtccaat tcatcgggaa agatggagag
     7261 aattttgata tccagtgttc tcccagtggt tttgaccggg aaggagagtt gatgttgtca
     7321 ttgttttaca ataaaacagt tattgcccgg ttaacatttt cagtcattct gactcaaaat
     7381 ggacacattg cttttattgg cggattacag ggagccccta aaaataccgg acctgatgtt
     7441 attcggtgcg caacccgggc ctgttatggg attttcccaa aacggattat ttttgaagca
     7501 ttttgtgcgt tgatgaaagc ttgcaatatc tctgagtgtc tggcagtcag tgagcacagt
     7561 catgttttcc ggcaattgag atactggtat cagaaacgca aaacctttgt tgcggtctat
     7621 agtgatttct gggagtctgt agcaggaaaa acctgtggtg actggtacag attaccaacc
     7681 caggtgattc gaaagccgct aagcgatata gccagtaaaa aacgctctga gtaccgaaaa
     7741 cgatacgctt tgctggatta tatccatgaa actactatca gctctttgga tgcatatcct
     7801 gtacactcag aacatcagaa tttaaattaa ggtttgatgt attctctgaa taaaacagta
     7861 atgataattt tggtagaggc attcgcacta aataatgaaa aaatataaat ctgagtttat
     7921 tcctgaattt aagaaaaatt acctttcccc tgtttactgg tctacatggt tccttttggg
     7981 aatgattgca ggtatttcaa tgtttccccc ttcattcaga gatcctgtct tggcaaaaat
     8041 agggcgttgg gcgggcagat tgagcaaaaa agctcgtcgc agggcgacga ttaatttatc
     8101 gctttgtttc ccagaaaaga gtgatacaga acgggaaata attgtcgaca aaatgtttat
     8161 cacagcattg caatccatag tgatgatggc agaactggca attcgtggtc cggaaaagtt
     8221 ccagaaacga gtgttctgga aaggcctcga aattcttgag gaaattcgac acaataatag
     8281 aaatgtgatt tttctggttc cccatggctg gagcgtggat attcctgcaa tgttgctggc
     8341 agctcaggga aaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta
     8401 tgtctggaat tcagtacggc gtaaattcgg ggggcgctta catgcccggg aggatgggat
     8461 aaaaccattt attcagtcag tacgccaggg atactgggga tattacctcc cagatcagga
     8521 tcatggtcct gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc
     8581 aatcattgga cgtctgatga acatcagtca ggctatgatt ataccacttt tcccggttta
     8641 tgatgaaaaa aaacatctac tgactattga aattcggcca ccaatggatg cttgcattgc
     8701 cagcgcggac aataaaacga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg
     8761 gccacatccg gaacagtatg tctgggtttt aaaattgtta aaaacacgca aatcaaacga
     8821 agcggacccg tacccttgaa attataaagt acaagtattt ccactagttg tttgtaagcg
     8881 tacagcctga accgtctggt cagaatctga cgaattagac aaagtggtgt ccaccaaata
     8941 agtagtggga accaatgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg
     9001 gcatcagaag gagctaataa tgagcagaaa aacccaacgt tactctaaag agttcaaagc
     9061 cgaagctgtc agaacggttc ttgaaaatca actttcgatc agtgaaggcg cttcccgatt
     9121 atctcttcct gaaggcactt taggacaatg ggttaccgcc gccagaaaag ggctcggtac
     9181 tcctggttcc cgcacggtgg ctgaactgga atctgaaatt ctgcaactgc gtaaggcgtt
     9241 aaatgaagct cgccttgagc gagatatatt aaattgcaca ggagtcgctg aaaaatacgc
     9301 gttaatcgaa caatggcgac aacaatttcc cattgaagcg atgtgtcagg tatttggtgt
     9361 atccaggagc ggttattaca actgggtaca gcatgaaccc tcagacagaa aacaaagtga
     9421 tgagcggcta aaactggaga ttaaggtggc acatatccgc actcgcgaaa catatggaac
     9481 ccggcggctc cagacggagc tggcagagaa tggcatcatc gttggtcgtg accgactggc
     9541 acgtcttcgt aaggagctaa ggctacgctg taagcagaaa cgcaagttca gagcgactac
     9601 gaactcgaac cacaatctgc cagttgcgcc aaatctgctg aaccagacgt tcgctcctac
     9661 agcaccaaat caggtctggg tggcggacct gacgtatgtt gccacacagg agggatggtt
     9721 gtacctcgct ggcatcaaag atgtttatac gtgcgaaatt gtcggctacg ccatgggaga
     9781 gcgcatgaca aaagagctga caggtaaagc cctgtttatg gcgctcagga gccagcgccc
     9841 acctgccggg ctaatccacc actctgatcg aggttcacag tactgcgcat acgattaccg
     9901 ggtcatacag gagcagtttg gtctgaaaac atcaatgtcg cgtaaaggta actgttacga
     9961 caacgctccg atggaaagct tctggggaac gctgaaaaat gagagcctga gccactatcg
    10021 ttttaataac cgggatgaag ccatctcagt aatacgggaa tacattgaga ttttctacaa
    10081 tcgtcagcgt cgtcactctc gtctggggaa tatctccccg gcagccttca gggaaaaata
    10141 tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct
    10201 caacattcca ccatgcatta cgattaacgc cgcatagcca gttgaacttt gctactttgt
    10261 gagaggtagt accttctatc cagtgcgaat ttaattaatg gaataaatga ttatgagtga
    10321 aaatgataca atcccaaaga agtctacaag tcagattaac aaagcggtat tctttacatc
    10381 tgctttgcta attttccttc ttgtcgcctt tgccgccgta ttcccggatg tcgccgacaa
    10441 aaattttaaa ctacttcagc aacaaatctt cacgaatgcc agctggttct acatccttgc
    10501 tgtggccctg attttactga gtgtcacgtt ccttggactc tcacgctacg gtgatatcaa
    10561 gctgggcccg gaccatgcgc agcctgattt cagctaccac tcctggtttg cgatgctttt
    10621 ttcggcaggg atggggatcg gcctgatgtt ctttggcgtt gccgaacctg taatgcatta
    10681 tctttcgcca cccgttggca ctccagaaac cgttgcggca gctaaggaag caatgcgtct
    10741 gacctttttc cactggggac tgcacgcatg ggcaatttat gccattgtgg cgctgattct
    10801 ggcgttcttc agttaccgtc acggtctgcc tttaactctg cgctccgcac tctatcccat
    10861 tattggcgat cgcatatacg gacctgtagg acatgcggtt gatattttcg ctgttatagg
    10921 cacggtcttt ggcgttgcga catcactggg ttacggtgtt ttgcaggtga atgccggttt
    10981 gaaccatctt ttcggggtgc ccatcaatga aacggtgcag gtaattctga tcgtggtcat
    11041 cacggggtta gcgacgattt cagtggtgtc cggtctggat aagggaatac gtatcctgtc
    11101 tgaactcaat ctgggtctgg ctttgttgct cctggcgctg gtcctgtgtc tgggaccaac
    11161 cgtgcttctg ctgaagtcat ttgtggaaaa tacgggcggt tatctttcgg aactggtgag
    11221 taaaacgttc aacctttacg cgtatgagcc caagtcgagc aactggctgg ggggctggac
    11281 attactgtac tggggatggt ggctttcatg gtcgccgttt gtggggatgt tcatcgcacg
    11341 ggtctcccgc gggcgaacca ttcgcgagtt tgtcaccggc gtgctgtttg ttcccgcggg
    11401 ttttacgcta atgtggatga cggtgtttgg taacagcgcg atctatctca ttatgaacca
    11461 gggggccaca gacctcgcca agactgttca gcaggatgtg gcgctggccc tgtttaattt
    11521 cctggagcat ttcccgttct cttctgtgct gtcattcatt gcaatggcga tggtcatcgt
    11581 cttctttgta acgtctgctg attcgggggc aatggttgtg gatactctgg catcaggtgg
    11641 agtggcaaac acacccgtct ggcagcgaat attctgggcc tcgctcatgg gcattgttgc
    11701 aattgcgctt ctccttgccg gagggctaag tgcgctgcaa acggtgacaa tagcgagtgc
    11761 attgcccttc tcagtgatct tactaatatc tatatacgga cttttaaaag ctttgcgccg
    11821 ggatttgacc aagcgtgaaa gcctgagcat ggcgacaatt gctcctacgg ctgcacgtaa
    11881 cccaattcct tggcagagaa ggttacgcaa tatcgcgtat ctgccgaagc gatctcttgt
    11941 gaaacgtttt atggacgacg ttatccagcc tgccatgacg ctggttcagg aggaactgaa
    12001 caagcagggg acgataagcc acattagtga tgcagtcgac gatcgtattc gtcttgaagt
    12061 cgatttgggc aacgagctga attttatata tgaagtgagg cttcgcgggt atatctcacc
    12121 gaccttcgcg ctcgccgcaa tggataatga tgagcagcag actgaacaac atcgatatta
    12181 tcgcgctgag gtttatctca aagaaggcgg tcaaaattat gatgtgatgg gctggaacca
    12241 ggaacagctg attaatgaca tactggacca gtacgaaaaa cacctgcact tcctgcacct
    12301 ggttcgttaa tagcaacatg ccgtcctggg ggcggcaatt attatcccgg ccgcaatatg
    12361 agggaatgca gaatgatttc acgctggaaa tggatgctga agcagacaat taaaaaacta
    12421 tggttcaggg caacgttatt cgcaattgtc gcgataataa cggccctttt atcaattctt
    12481 tttaaatcaa tgatacctga gtcggtttcc gtgaaggttg gtgcggaagc agtcgataac
    12541 attctgaaca tactggcatc gagtatgctg gcagtgacca cattttcgct gagtatcatg
    12601 gtcacagcct acggttcagc cactactaat gtgactccca gagctacgcg tttagttgtt
    12661 gaagacgtca ccacacaaaa tgtactggcc accttcatcg gttcttttct cttcagtctg
    12721 gtagggatta ttgccctcaa tatgggagct tatggagaaa gggggagagt cattttattc
    12781 attgtcacac tggttgtcat tgccttaatc ctcatcacat tgcttcgctg gatacagcat
    12841 ttgacctctc tggggagggt tggtgagaca acggcacaag tagaacaggc ggccatcgaa
    12901 acatttattg cgagagcaag aaatccctgt ctcggtggat atccatggct tgagaacaat
    12961 gaacagccga aaggaacggt tgcagtttat ccgaagaaga ttggctatgt tgaatatatt
    13021 gatatggtga aacttagcaa gctgctgacc aatgatcccc gtcatgtata cctcgtggcg
    13081 cagccaggca gtttcataca tccgtccatg ccagttttgt acctgagtca gggccaggag
    13141 tcgtcaatca acaccgattt acttgagacg attattgtct cggatgcacg ttcatttgct
    13201 caggatcctc gattttgtct tagcgtcatg gccgaaatag cctgccgggc cctttcccct
    13261 gcagtgaacg atcctggaac cgccattgat gtcattggca gaggtgttcg tatactttcc
    13321 acttacgcgc agaataaatc tgatgaaata gaagtgaaat atccttcagt acatgttgca
    13381 ccacttcaga ataacgatct actggaagac tttttctcac ctgtcgcgcg cgatggtgcc
    13441 agtatgaggg agattcagat aagagtcctt aaaggactgt cgatgctgag taaaggttgg
    13501 cctgagatat ttgctgaagc cgcacatacc ctggcatttg aaacattaga gcatgcaact
    13561 cgtgctgacc atatagattc tgatagatat ctaataaaat caacttatta taatttattt
    13621 agtggcgaat attctaataa aaaaacatag ctgcagagca agcaattcgg ttgttggcaa
    13681 gtcaagggca aagcattcgc gtgagaatgg gcgtatgctt tgtgccaaaa gcaaacgttg
    13741 ctaattctgc actgttcagc gatggcgcag tagagacagg cactgccact gtccagtcgg
    13801 caaaagcgaa cactttgcca ctgcggggat aaactgattg aagctctcgc gcaaaatcac
    13861 ttattctttt gcgttaataa aatgtaggag atgggtcatt gatcctaccc acgtaatatg
    13921 gacacaggcc taagcgaggt tcttgttttc aaattgttcc ggactgaggc cgccacacca
    13981 actgtgccgc cgccaccgat tgtaatcaca ttcgatataa ttaaacaccg ttgcccgcat
    14041 tatttcccgg ctgataaagt gttctccatg gatacattcc actttcagcg aatgaaagaa
    14101 gctttccacg caggcattat cgtagcagca accttttgcg ctcatacttc cacgcagatt
    14161 atgccgcttc agttgcgcct gataatctgc tgaacagtac tggcctccac ggtccgtgtg
    14221 aacgataacg ttccggggcc tcgtacgccg ccatagcgcc atctgcaggg catcgcaggc
    14281 cagttgcgcc gtcatgcgtg gcgacattga ccagccaata acggcacgtg accacaggtc
    14341 aatgaccact gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcctgc
    14401 ccacttctgg ttcgggccac tggtgtaaaa atcctgctcc aacagatttt ctgatacagg
    14461 caggccgtgt gcgcggtagc tgaccgggct gaacttccgg gaggcctttg ccctcagtcc
    14521 ctgacggcgc aggcttgccg ccacggtttt tacgttaaag gggtaaccct gagcacgcag
    14581 ttcatccgtc aggcgtgggg caccgtaacg ctgttttgac cgggtaaaag ccgcgaggac
    14641 aacgctgtcg cagtgttggc ggaactgctg acgcgtgctt atccttgtcc gccgctgaca
    14701 ccacgtatac cagccgctgc gggccacccg gagcacgcgg cacattgctt tgatgctgaa
    14761 ctcagcctga tgtttttcaa taaagacata cttcatttca ggcgcttcgc gaagtatgtc
    14821 gcggcctttt ggaggatagc cagctcttca tcccgttctg ccagctggcg tttgagacgt
    14881 gcaatctcgg tagacatctc cagttcacgt tcagaagacg tctgctgatt ttgctgttta
    14941 ctgcgccagt tgtagagctg tgattcatac aggctgagtt cacgggctgc ggcagtaaca
    15001 ccgatgcgtt cagcaagctt cagggcttca ctgcgaaatt caggcgaatg ctgtttacgg
    15061 ggttttttac tggttgatac tgtttttgtc atgtgagtca cctctgactg agagtttact
    15121 cacttagccg cgtgtccact attgctgggt aagatcacaa cactttaaaa tgccctatat
    15181 agggttaacc agtaattatc tgtatgatag ctttgagttc gggaggaggt aatgactcca
    15241 acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg cagctccacc
    15301 gattttgaga acgacagtga cttccgtccc agccttgcca gatgttgtct cagattcaga
    15361 ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa cgtgcagctt tcccttcagg
    15421 cgtgattcat acagcggcca gccatccgtc atctatacca cgacctcaaa ggccgacagc
    15481 aggctcagaa gacgctccag tgtggccaga gtgcgttcac cgaagacgtg cgccacaacc
    15541 gtcctccgta tcctgtcata cgcgtaaaac agccagcgct gacgtgattt agcaccgacg
    15601 tagccccact gttcgtccat ttcagcgcag acaatcacat cactgcccgg ttgtatgcgc
    15661 gaggttaccg actgcgacct gagtttttta agtgacgtaa aaccgtgttg aggccaacgc
    15721 ccataatgcg tgcactggcg cgacatccga cgccattcat ggccatatca atgattttct
    15781 ggtgcgtacc gggctgagag gcggtgtaag tgaactgtag ttgccatgtt ttacggcaat
    15841 gagagcagag atagcgctga tgtccggcag tgcttttgcc gttacgcacc acgccttcag
    15901 tagcggagca ggaaggacat ctgatggaaa tggaagccac gcaagcacct taaaatcacc
    15961 atcatacact aaatcagtaa gttggcagca ttaccgaaat ccccatcctt tagggcagtg
    16021 aggatgtcaa taatagcaga ccacatcatg agtcattctg accgacacat gccagaagca
    16081 gaggctccat cccattttat acctacctta taacacttag aaaaacaaca tgttaaaaat
    16141 gtctattgga aacaatttta tttccaattg taatgataac cattctcata ttaatatgac
    16201 tacgtgataa ttaacttttg atgcactccg catgtctgac cgcgccacta ccacagcttc
    16261 cttaacgttc gagtcgcttt atggcacaca tcacggctgg ttgaaaagct ggctgacgcg
    16321 caaactccag tctgcttttg atgcagatga cattgcccag gacacttttt tgcgggtaat
    16381 ggtcagcgaa acgctctcga cgatccgcga tcctcgctcc ttcctctgca ctatcgccaa
    16441 acgcgtgatg gtggacctgt ttcgccgaaa cgcgctggaa aaagcgtatc tggagatgct
    16501 ggcgcttatg ccggaggggg gagcgccttc acctgaggaa cgcgaaagcc aactcgagac
    16561 cctacaactc ctcgacagca tgctggacgg gctaaacggc aaaacacgtg aagcgtttct
    16621 gctttcgcaa ctggatggtc tgacatacag cgagattgcg cacaaactcg gtgtttccat
    16681 cagctccgtg aaaaaatacg tggcgaaagc cgtcgagcac tgcctgctgt tccgtctgga
    16741 gtatgggtta tgaatccttt gttaaccgat tcccgccgtc aggcgctgcg ttcagcttcc
    16801 cactggtatg ccgtgctaag cggcgagcgc gtcagcccac aacaggaagc gcgctggcaa
    16861 cagtggtatg aacaggatca ggataaccag tgggcctggc agcaggttga aaacctgcgc
    16921 aaccagcttg gcggtgtgcc tggcgacgtt gccagccggg cgttgcacga tacccgcctc
    16981 acccgccgtc acgtgatgaa aggattactg ctgttgctcg gcgctggcgg aggctggcag
    17041 ctctggcagt cggaaaccag cgaaggtctg cgggcagatt accgcaccgc caaaggcaca
    17101 gtcagccgcc agcaactgga agatggctcc ctgctcacgc tgaataccca aagcgcggcg
    17161 gatgtgcgtt ttgatgcgca tcagcgcacc gtccggctct ggtacggtga aatcgccatt
    17221 actaccgcga aagatgccct gcaacgcccc ttccgtgtcc tgacccgtca gggccagctc
    17281 actgctttag ggacagaatt taccgtccgc cagcaggata atttcacgca gcttgacgtg
    17341 cagcagcacg ctgtggaagt gcttctcgcc agtgcccccg cgcaaaaacg catcgtgaac
    17401 gctggtgaaa gcctgcagtt cagcgcctct gagtttggcg cagtgaaacc gctggatgac
    17461 gagagtacaa gctggacgaa ggacatcctg agcttcagcg ataaaccgct gggtgaggtg
    17521 atagccacgc taacccgtta ccgcaacgga gtgctgcgct gcgatcccgc cgttgccggg
    17581 ctgcgcctga gcgggacgtt cccgctgaaa aataccgatg cgatcctgaa cgttatcgcg
    17641 caaacgcttc ccgttaaaat tcagtctatt acgcggtact ggataaacat ttcaccactg
    17701 taaggaaaat aattcttatt tcgattgtcc tttttaccct tctcgttcga ctcatagctg
    17761 aacacaacaa aaatgatgat ggggaaggta tgacgccgtt acgcgttttt cgtaaaacaa
    17821 cacctttggt taacgccatt cgcctgagcc tgctgccgct ggccggtctc tcgttttccg
    17881 cttttgctgc acaggttaat atcgcaccgg gatcgctcga taaagcgctc aatcagtatg
    17941 ccgcacacag cggatttacc ctctcggttg acgccagcct gacgcgcggc aagcagagca
    18001 acggcctgca cggcgattac gacgtcgaga gcggcctgca acaactgctg gacggcagcg
    18061 gactgcaggt aaaaccgctg ggaaataaca gctggacgct ggagcccgcg cccgcaccaa
    18121 aagaagatgc cctgaccgtg gtcggcgact ggctgggtga tgcgcgtgaa aacgacgtat
    18181 ttgaacatgc tggcgcgcgt gacgtgatcc gccgtgagga tttcgccaaa accggcgcaa
    18241 ccaccatgcg tgaggtactt aaccgcatcc ctggcgtcag cgcgccggaa aacaacggca
    18301 ccggcagcca cgacctggcg atgaactttg gcatccgggg cctgaaccca cgcctcgcca
    18361 gccgctcgac cgtcctgatg gacggcatcc ccgtcccctt cgccccttac ggtcagccgc
    18421 agctttcact ggctcccatt tcgctcggca acatggatgc cattgacgtg gtacgcggtg
    18481 gtggtgcggt gcgttacgga ccgcagagcg tgggcggcgt ggtgaacttt gttacccgtg
    18541 ccattccgca ggactttggt atcgaggcgg gcgtggaagg tcagctcagc ccaacctctt
    18601 cacaaaacaa cccgaaagag acgcacaacc tgatggtggg cggcacagcg gacaacggtt
    18661 ttggcaccgc gctgctctac tccggcacgc gcggcagtga ctggcgcgag cacagcgcca
    18721 cccgcatcga cgacctgatg ctgaaaagca aatatgcgcc ggatgaggtg cacaccttca
    18781 acagcctgct gcaatattac gacggtgaag ccgacatgcc cggtggcctg tctcgcgcgg
    18841 attacgacgc cgatcgctgg caatccaccc gcccgtatga ccacttctgg ggtcgtcgca
    18901 agctggcgag cctgggctac cagttccagc cagacagcca gcataaattc aacattcagg
    18961 ggttctacac ccaaaccctg cgcagcggct acctggagca aggcaaacgc atcaccctct
    19021 cgccgcgtaa ctactgggtg cgcggtattg agccacgcta cagccagatc tttatgatcg
    19081 gcccttccgc gcacgaagtg ggcgtgggct atcgctatgt gaatgaatca acgcatgaaa
    19141 tgcgttacta caccgccacc agcagcgggc agttgccgtc cggctcaagc ccttacgacc
    19201 gcgatacgcg ttccggcacc gaggcgcacg cctggtatct ggatgacaaa atcgacatcg
    19261 gcaactggac catcacgccg ggtatgcgtt tcgaacatat cgagtcatac cagaacaacg
    19321 ccatcacagg cacgcacgaa gaagtgagct ataacgcacc gcttccggcg ttgaacgtgc
    19381 tctatcacct gactgacagc tggaatcttt atgcaaacac tgaaggctcg ttcggcaccg
    19441 tacagtacag ccagattggc aaggctgtgc aaagcggcaa tgtggaaccg gaaaaagcgc
    19501 gaacctggga actcggtacc cgctacgacg acggcgcgct gacggcggaa atggggctgt
    19561 tcctgattaa ctttaacaat cagtacgact ccaaccagac caacgacacc gtcactgcac
    19621 gtggcaaaac gcgccatacc gggctggaaa cgcaggcacg ttacgatctg ggtacgctaa
    19681 cgccaacgct tgataacgtt tccatctacg ccagctatgc gtatgtgaac gcggaaatcc
    19741 gcgagaaagg cgacacctac ggcaatctgg taccattctc cccgaaacat aaaggcacgc
    19801 tgggcgtgga ctacaagcca ggaaactgga cgttcaatct gaacagcgat ttccagtcca
    19861 gccagtttgc ggataacgcc aatacggtga aagagagcgc cgacggcagt accggccgca
    19921 ttcccggctt catgctctgg ggcgcacgcg tggcgtatga ctttggcccg cagatggcag
    19981 atctgaacct ggcattcggt gtgaaaaaca tcttcgacca ggactacttc atccgctctt
    20041 atgacgacaa caacaaaggc atctatgcag gccagccgcg cacgctgtat atgcaggggt
    20101 cgttgaagtt ctgatgtgtt aacgcccggc ttgccgggct tttagctgga atgtgattat
    20161 gttggcattt atccgttttc tttttgcagg cctgctgctg gtgatcagcc acgcctttgc
    20221 cgccatggtt caggacgaac acggcacgtt tacactcgaa aaaacgccac aacggattgt
    20281 ggtgctggaa ctctcgttcg ccgatgcgct ggccgccgtg gacgtcagcc cgatcggtat
    20341 tgccgacgat aacgatgcaa aacgcatcct gcccgaagtg cgtgcgcacc tgaaaccgtg
    20401 gcagtccgtc ggaacgcgcg cgcagccgag cctggaagcc attgccgctc tgaaaccaga
    20461 cctgatcatt gccgacagca gtcgccatgc gggggtttac atcgccttgc agcaaatcgc
    20521 gccggtactg ctgcttaagt cccgcaacga aacctacgct gaaaatttgc aatctgcggc
    20581 tatcatcggc gaaatggtgg gtaaaaagcg agagatgcag gcacgtctgg aacaacataa
    20641 agagaggatg gcgcagtggg ccagccagct tcccaaaggg acacgcgtgg cctttggcac
    20701 atcacgcgaa cagcaattca acctgcatac tcaggagacc tggaccggca gcgtgctggc
    20761 ctctctgggg ctgaacgttc ccgctgcgat ggcgggttcg tccatgccgt ccatcggcct
    20821 ggagcaactg ctggcggtca atcctgcctg gctgctggtt gcccactatc gcgaagagag
    20881 cattgttaaa cgctggcaac aagatccgct ctggcagatg ttaaccgccg cgcagaagca
    20941 gcaggttgct tcggtcgaca gtaacacctg ggcgcggatg cgcggtattt ttgctgcaga
    21001 gcgtattgcc gctgacacgg taaaaatctt ccaccatcag ccgcttaccg ttgtgaaatg
    21061 accgcgataa aacacccgat gctgctgtgg gggcttcccg ttgcagcact tatta
//
