LOCUS       NZ_CP060937             3374 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC12 plasmid pEC12-5, complete sequence.
ACCESSION   NZ_CP060937
VERSION     NZ_CP060937.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904656
            Assembly: GCF_022558865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558865.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:23:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,209
            CDSs (total)                      :: 5,085
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 74 of 257
            Pseudo Genes (incomplete)         :: 187 of 257
            Pseudo Genes (internal stop)      :: 43 of 257
            Pseudo Genes (multiple problems)  :: 43 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060937.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC12"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC12-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-08-09"
                     /collected_by="Long Sun"
     gene            complement(507..698)
                     /locus_tag="H9199_RS25925"
                     /old_locus_tag="H9199_25645"
     CDS             complement(507..698)
                     /locus_tag="H9199_RS25925"
                     /old_locus_tag="H9199_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            762..1085
                     /locus_tag="H9199_RS25930"
                     /old_locus_tag="H9199_25650"
     CDS             762..1085
                     /locus_tag="H9199_RS25930"
                     /old_locus_tag="H9199_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1294..>1731
                     /locus_tag="H9199_RS25935"
                     /pseudo=""
     CDS             1294..>1731
                     /locus_tag="H9199_RS25935"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1763..2323
                     /locus_tag="H9199_RS25940"
                     /old_locus_tag="H9199_25660"
     CDS             1763..2323
                     /locus_tag="H9199_RS25940"
                     /old_locus_tag="H9199_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2330..2563
                     /locus_tag="H9199_RS25945"
                     /old_locus_tag="H9199_25665"
     CDS             2330..2563
                     /locus_tag="H9199_RS25945"
                     /old_locus_tag="H9199_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          343..426
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="95.0"
                     /evalue="4.11e-16"
     Typing          1076..2646
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.628"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actacccgtc ccttcactgg gaggggtgag ttgttgccgt tccctccgac cttccaccat
       61 ccagcatatc gcacgatccc atgtgcaccg ctactcctga taactccagt gcaaaaaaaa
      121 aaaacggatc tgcatcacaa cgagagtgat cccaccgctc gccgcagccc cagcgaccga
      181 acgtagtgag cgagtgggcg aggaagcgga agagtcacag aggcaagcat tgtgcactta
      241 cgcgcctgtg ccagtgtccg cgtgtgtgtt gtttatcggg cgcgctgttt ttacgcatct
      301 gtgcggcttt ttactcctga catggtgcac ttcccatcca ctctgcaccg atgcaaaatg
      361 aggaaagcca gtatacacat cgctatcgct ctgtgaactg ggtcagggct gcgccccgac
      421 acccgcactg acgcgccctg gcgggcttgt ctgctcccgg catccgctca caggcaagct
      481 gtgaacgtct ccgggagctg catgtttcag ttgctttcac cgtcatcgcc gaagcgtgcg
      541 aggcaactgc ggtagagttc gtcggcgtgg tcgtgaagtg attcgcagat gtctgcctgt
      601 tcgtccgcgt ccagatcgtt gagtttttcc agcagggtta gcgtctggtt tctgataaag
      661 cgagccatgt taagggctgt tttttcctgt ttggtcatgg tcttaaggct ccttgtatgg
      721 gggatttgtt agggggaatg ataccgatga aacgcgagaa gatgctcacg atacgggtta
      781 ccgatgagga gcacgcccgg ttactggaac gctgcgaggg taaacgactg gcggaatgga
      841 tgcggcgggt ctgtctgggg gagccggttg cacgcacagg aaaactgccg acactggcac
      901 cgccattgct gcggcatctg gctgccatcg ggaacaacct gaaccagaca gcccggaagg
      961 tgaacagcgg ccagtggtct tcgattgacc gggttcatgt ggtggccgca ctgatggcca
     1021 tcgagggtga attgcgccag ttgcggcagg ctgtcaggga acagggggta cgggatgata
     1081 gttaaatttc atgccagggg gaaaggcggt ggaagtggcc cggtcgatta cctgctgggg
     1141 cgggagcgta accgcgaagg cgcaacggtg ctccggggta acccggaaga agtgcgggaa
     1201 ctcattgatt ccacgccatt ttcgaagaaa tacacgtcag gcgttctgtc gttcgcggag
     1261 aaggagctac caccgggtga acgcgaaaga gtgatgacga gctttgagcg tgttcttatg
     1321 cccggtctcg aaaaagacca gtacagcatt ctctgggtgg aacaccagga taaggggcgg
     1381 ctcgaactga attttgtcat cccgaacatg gagctacaga gcggtaagcg cctccagccg
     1441 tactacgacc gtgcggacag gccgaggata aatgcctggc agacgctggt taatcatcat
     1501 tacgggctgc gtgacccgaa cgcaccggag aacagacgga cgcttgtcac gccaaataat
     1561 ctgccaaaag cgaaacagga ggccgcagaa gcgattacac ggggtcttga ggctctttac
     1621 catgccggag caataaaaac gcgtcaggat gtcacagagg cgcttacagc ggcaggtttt
     1681 gaggtggtga ggacaaccaa aagcagcatc agtattgccg acccggaggg ggggcggaat
     1741 ctcaggctaa agggggcgct gtatgagcaa tcttttacaa atgggaacag acttcgagaa
     1801 gaaactgaaa gagcgagccg cttctaccga gaacatgctg aacagcgagt tcaggaagct
     1861 cgaagagtct gtagacaagg cattgagctt aaacaggcag aaaatcagag acgctatcag
     1921 cgaacataca acatccgtga aaaagcaact ggatacgctg agcaccaccg tgagcatgca
     1981 gttcagcacc acagaggccg aactgtcccg gcagcagaaa aaactgctct ggcgggtgat
     2041 caaagggcgg atactgtttc cggcgctgac cgcgctcagc gtcacgggcg ggattttcct
     2101 ggggtgctgg gggctgatgg agtggcagga aagcaagatt gcgaaaaata tcctgactat
     2161 cagagagcag gaaaacacgc tggcgaaact cgaggcaaaa acctgggggg tgacgttcgt
     2221 caatggcgag aacgggaaat ttctggtgtt gccggacggg gtgaaagggg aaaacacctg
     2281 gacggtgggc gacaagaacg ccgtgaggct ggtcagggag taaacgagta tgacggaact
     2341 ggaagggcat ttgctgaacg ccttagagca tttgcaacag gactatatgc ggcggctgaa
     2401 cgagtgggag agcgccttcg cggaattgca gaagatgcac gaggttacgc aacggaacaa
     2461 cgcgatactg aacgagcgcg tcgtgatatt gagtcagcag gtgcagagat tagccgggca
     2521 agtggatcgc ttgagtcggt tatttatcgc gaacaacagc taagggatga acaggagcgt
     2581 gcccggtaca gacatgaggt acaggaacgc gcggcacggg ttcggagtgg gccatcaatg
     2641 gagatgtgac cggagaagtg acgaaatctt gccattttga cccgttcgac ggctggggcg
     2701 agcgaaacaa atcgtgtgcc gtatttgtgc atagctatgc aacatcctga aaacgcattt
     2761 cacgcgtttt tggggattca gccaggggaa aacatggttt ttttactttt tgagcgtaaa
     2821 gacgtgaaaa atctcgttat tgcacttgcg atctttcgtc ggtgggcgta gaacgaaaac
     2881 agctcgccag gaaggcgagc tgtttcgggt tcattgaggt ctctgacctc ctcaatgttc
     2941 ctgtggaaac tgttgagatc cacaacagaa accagttcat tcagtttgca tgagccttct
     3001 gtattcttca agagtttttc aacagcacag tctcccagga accccgaaaa aaacctgttt
     3061 ttgcagatac ttgcgtattt gcgagaatcg tttgcggttg ggggagctgt gacgacggcg
     3121 accgcacgcg tgagagttcc gctaccggcg cagagagttt catcgacagc ggacacctga
     3181 tgagttgcca caaaaaccgt atgtagcgcg gctagattgg ttgagttaga ccttggcggc
     3241 agttcccggt atgtggtggc gtgacgggtg agatggtgcg gcgaagacgc atacgtcacg
     3301 caggcgcggg ttcttccact gacgcgcccc gtgatcgatg gccttggctc cgtccggcat
     3361 atcgagagcg gcaa
//
