LOCUS       NZ_CP060932             2045 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC13 plasmid pEC13-5, complete sequence.
ACCESSION   NZ_CP060932
VERSION     NZ_CP060932.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904657
            Assembly: GCF_022558885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558885.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:20:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,951
            CDSs (total)                      :: 4,827
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 73 of 251
            Pseudo Genes (incomplete)         :: 184 of 251
            Pseudo Genes (internal stop)      :: 43 of 251
            Pseudo Genes (multiple problems)  :: 44 of 251
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060932.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC13"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC13-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-09-28"
                     /collected_by="Long Sun"
     gene            complement(870..1211)
                     /locus_tag="H9200_RS24645"
                     /old_locus_tag="H9200_24410"
     CDS             complement(870..1211)
                     /locus_tag="H9200_RS24645"
                     /old_locus_tag="H9200_24410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039819495.1"
                     /translation="MPLKGNGNKFINNGIGVHNEAQVSVELNDSTFENNKIGYLEGKLD
                     RIDELFKNETPELAKKELIEELSKAIKRGESEKAESIFEKFDFHKWIASGANIAKIVES
                     LASAMNIHS"
                     /name="hypothetical protein"
ORIGIN
        1 ggttgaaaaa taatcaacca tggtgggtga tttacgcagg ttaaggggag atcgtgacaa
       61 aaaaagcgtt cctcactgta tcgttcatgc aggcatgaac gggttatcct ccccgactac
      121 agatagacta caaaagctaa gatgagacta cagataaact acagaagctg atgctggaca
      181 ggcattcacc tggtataaga ctacagaatt acatctctcc ttcagcttat acgtcacttt
      241 tgcgtcattt ttacatcact ccttaagttc atacgtcact tttacgtcag ttccatcctc
      301 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
      361 cggcaaggtc tcacagttac gcaataaatt gcccgcccgt tcaccgtttt aggcaaggcg
      421 aaagatactc ttttttgggg ttgggtttga attcaggccg aaacctgatg ccaggcagcc
      481 ggctgcataa cgattcacag cctcagaaat catcagcgcc attctgagcc gttttcaggg
      541 ggtaagacat gcggttttga ggttcaggcg ggaaaatcgc gcacagcccg tctgaggggt
      601 gcgcgcaatg agacgttatg gtaaattcag aaagacaatg tggggggggt atgatttcaa
      661 taattaattg cacagctgaa agctgcggca ttgggtacag tatagattcg tcagctgatg
      721 tttatatgtc tggtaatgag gccattaatt gtggagtggg cctgcatatc aacgatcgtg
      781 aaatccgtca ggctcagaaa gcaaagttac ctgacgggca tttcaatatg agtattgtgg
      841 cgtatttggt tcgagtatat ttacaaaggt tacgaatgaa tgttcatagc agacgctagc
      901 gactcaacga tctttgcaat gttagcgcca gaggctatcc acttgtgaaa gtcgaatttt
      961 tcgaaaatag actctgcttt ttctgactcc ccacgcttta tagccttgct aagctcttct
     1021 attaactctt ttttagcgag ttccggcgtt tcatttttga agagttcatc tatgcgatcg
     1081 agcttaccct ctaagtaccc tattttatta ttttcaaaag tagaatcatt aagctccacg
     1141 ctgacttggg cttcgttgtg aacgccgata ccgttgttaa tgaatttatt tccattacct
     1201 tttagcggca tagttcctcc agttttggtt aaagttagcc taatgccccc gtggtgggta
     1261 tataagacca cggggagcgt agcgaccttc gtcaagctct cttcttgtgt gtctcacctt
     1321 gcaagggctt gattgtgtgg gtgtcgtgtt cgactttctt gcaatcacac gccaccgctt
     1381 actactgcct tagtcgctca ttttatcagg tgcttgttat cgtacagcga gacggggtag
     1441 agcctaactc tttcgcggtt aagttacgtt cccaccaatt tgcctgttta tcctgccgga
     1501 gtccggggcg tagacctgag cgtctaaggt gcagatatga ttttaatgtc gttgaatggt
     1561 attaagctgg tagtgacatg gctagccagc cctgctaaga tttgattgct acgtctgatc
     1621 ttggcaggta tctgcaagaa ttagagtacc gaagccctgt gattctgtca cggggctttt
     1681 gtacatttaa agcatctaca tcaaaaccat cctttccaaa cctaagctga caacgctcgc
     1741 cgcagtactg actagcggag tctgtcagtg cgaggaagcg tcatatctgc cttgctgaga
     1801 taagcactgt cacacagagt ctgaacgtga acaaacaaca aaagtcagcc ctgaacatgc
     1861 cgaaatttat ccgtggccag ccagtaatac cccggtatta ctctttattt ctttattttc
     1921 atgtaattac agtggatttt taatacctgg gtattactcg tgaaaacgaa atgttccttt
     1981 tttttctttt aattcatgca gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat
     2041 tttga
//
