LOCUS       NZ_CP060923             3374 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC15 plasmid pEC15-5, complete sequence.
ACCESSION   NZ_CP060923
VERSION     NZ_CP060923.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904659
            Assembly: GCF_022558845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558845.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:04:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,173
            CDSs (total)                      :: 5,048
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 74 of 259
            Pseudo Genes (incomplete)         :: 188 of 259
            Pseudo Genes (internal stop)      :: 44 of 259
            Pseudo Genes (multiple problems)  :: 43 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060923.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC15"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC15-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-12-01"
                     /collected_by="Long Sun"
     gene            complement(<91..558)
                     /locus_tag="H9202_RS25980"
                     /old_locus_tag="H9202_25480"
                     /pseudo=""
     CDS             complement(<91..558)
                     /locus_tag="H9202_RS25980"
                     /old_locus_tag="H9202_25480"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(548..871)
                     /locus_tag="H9202_RS25750"
                     /old_locus_tag="H9202_25485"
     CDS             complement(548..871)
                     /locus_tag="H9202_RS25750"
                     /old_locus_tag="H9202_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            935..1126
                     /locus_tag="H9202_RS25755"
                     /old_locus_tag="H9202_25490"
     CDS             935..1126
                     /locus_tag="H9202_RS25755"
                     /old_locus_tag="H9202_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2444..2677)
                     /locus_tag="H9202_RS25760"
                     /old_locus_tag="H9202_25495"
     CDS             complement(2444..2677)
                     /locus_tag="H9202_RS25760"
                     /old_locus_tag="H9202_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2684..3244)
                     /locus_tag="H9202_RS25765"
                     /old_locus_tag="H9202_25500"
     CDS             complement(2684..3244)
                     /locus_tag="H9202_RS25765"
                     /old_locus_tag="H9202_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1208..1288)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(2..558)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.387"
                     /coverage="100.0"
                     /evalue="1.05e-119"
ORIGIN
        1 tgctccggca tggtaaagag cctcaagacc ccgtgtaatc gcttctgcgg cctcctgttt
       61 cgcttttggc agattatttg gcgtgacaag cgtccgtctg ttctccggtg cgttcgggtc
      121 acgcagcccg taatgatgat taaccagcgt ctgccaggca tttatcctcg gcctgtccgc
      181 acggtcgtag tacggctgga ggcgcttacc gctctgtagc tccatgttcg ggatgacaaa
      241 attcagttcg agccgcccct tatcctggtg ttccacccag agaatgctgt actggtcttt
      301 ttcgagaccg ggcataagaa cacgctcaaa gctcgtcatc actctttcgc gttcacccgg
      361 tggtagctcc ttctccgcga acgacagaac gcctgacgtg tatttcttcg aaaatggcgt
      421 ggaatcaatg agttcccgca cttcttccgg gttaccccgg agcaccgttg cgccttcgcg
      481 gttacgctcc cgccccagca ggtaatcgac cgggccactt ccaccgcctt tccccctggc
      541 atgaaattta actatcatcc cgtaccccct gttccctgac agcctgccgc aactggcgca
      601 attcaccctc gatggccatc agtgcggcca ccacatgaac ccggtcaatc gaagaccact
      661 ggccgctgtt caccttccgg gctgtctggt tcaggttgtt cccgatggca gccagatgcc
      721 gcagcaatgg cggtgccagt gtcggcagtt ttcctgtgcg tgcaaccggc tcccccagac
      781 agacccgccg catccattcc gccagtcgtt taccctcgca gcgttccagt aaccgggcgt
      841 gctcctcatc ggtaacccgt atcgtgagca tcttctcgcg tttcatcggt atcattcccc
      901 ctaacaaatc ccccatacaa ggagccttaa gaccatgacc aaacaggaaa aaacagccct
      961 taacatggct cgctttatca gaaaccagac gctaaccctg ctggaaaaac tcaacgatct
     1021 ggacgcggac gaacaggcag acatctgcga atcacttcac gaccacgccg acgaactcta
     1081 ccgcagttgc ctcgcacgct tcggcgatga cggtgaaagc aactgaaaca tgcagctccc
     1141 ggagacgttc acagcttgcc tgtgagcgga tgccgggagc agacaagccc gccagggcgc
     1201 gtcagtgcgg gtgtcggggc gcagccctga cccagttcac agagcgatag cgatgtgtat
     1261 actggctttc ctcattttgc atcggtgcag agtggatggg aagtgcacca tgtcaggagt
     1321 aaaaagccgc acagatgcgt aaaaacagcg cgcccgataa acaacacaca cgcggacact
     1381 ggcacaggcg cgtaagtgca caatgcttgc ctctgtgact cttccgcttc ctcgcccact
     1441 cgctcactac gttcggtcgc tggggctgcg gcgagcggtg ggatcactct cgttgtgatg
     1501 cagatccgtt tttttttttt gcactggagt tatcaggagt agcggtgcac atgggatcgt
     1561 gcgatatgct ggatggtgga aggtcggagg gaacggcaac aactcacccc tcccagtgaa
     1621 gggacgggta gtttgccgct ctcgatatgc cggacggagc caaggccatc gatcacgggg
     1681 cgcgtcagtg gaagaacccg cgcctgcgtg acgtatgcgt cttcgccgca ccatctcacc
     1741 cgtcacgcca ccacataccg ggaactgccg ccaaggtcta actcaaccaa tctagccgcg
     1801 ctacatacgg tttttgtggc aactcatcag gtgtccgctg tcgatgaaac tctctgcgcc
     1861 ggtagcggaa ctctcacgcg tgcggtcgcc gtcgtcacag ctcccccaac cgcaaacgat
     1921 tctcgcaaat acgcaagtat ctgcaaaaac aggttttttt cggggttcct gggagactgt
     1981 gctgttgaaa aactcttgaa gaatacagaa ggctcatgca aactgaatga actggtttct
     2041 gttgtggatc tcaacagttt ccacaggaac attgaggagg tcagagacct caatgaaccc
     2101 gaaacagctc gccttcctgg cgagctgttt tcgttctacg cccaccgacg aaagatcgca
     2161 agtgcaataa cgagattttt cacgtcttta cgctcaaaaa gtaaaaaaac catgttttcc
     2221 cctggctgaa tccccaaaaa cgcgtgaaat gcgttttcag gatgttgcat agctatgcac
     2281 aaatacggca cacgatttgt ttcgctcgcc ccagccgtcg aacgggtcaa aatggcaaga
     2341 tttcgtcact tctccggtca catctccatt gatggcccac tccgaacccg tgccgcgcgt
     2401 tcctgtacct catgtctgta ccgggcacgc tcctgttcat cccttagctg ttgttcgcga
     2461 taaataaccg actcaagcga tccacttgcc cggctaatct ctgcacctgc tgactcaata
     2521 tcacgacgcg ctcgttcagt atcgcgttgt tccgttgcgt aacctcgtgc atcttctgca
     2581 attccgcgaa ggcgctctcc cactcgttca gccgccgcat atagtcctgt tgcaaatgct
     2641 ctaaggcgtt cagcaaatgc ccttccagtt ccgtcatact cgtttactcc ctgaccagcc
     2701 tcacggcgtt cttgtcgccc accgtccagg tgttttcccc tttcaccccg tccggcaaca
     2761 ccagaaattt cccgttctcg ccattgacga acgtcacccc ccaggttttt gcctcgagtt
     2821 tcgccagcgt gttttcctgc tctctgatag tcaggatatt tttcgcaatc ttgctttcct
     2881 gccactccat cagcccccag caccccagga aaatcccgcc cgtgacgctg agcgcggtca
     2941 gcgccggaaa cagtatccgc cctttgatca cccgccagag cagttttttc tgctgccggg
     3001 acagttcggc ctctgtggtg ctgaactgca tgctcacggt ggtgctcagc gtatccagtt
     3061 gctttttcac ggatgttgta tgttcgctga tagcgtctct gattttctgc ctgtttaagc
     3121 tcaatgcctt gtctacagac tcttcgagct tcctgaactc gctgttcagc atgttctcgg
     3181 tagaagcggc tcgctctttc agtttcttct cgaagtctgt tcccatttgt aaaagattgc
     3241 tcatacagcg ccccctttag cctgagattc cgccccccct ccgggtcggc aatactgatg
     3301 ctgcttttgg ttgtcctcac cacctcaaaa cctgccgctg taagcgcctc tgtgacatcc
     3361 tgacgcgttt ttat
//
